Article
Biochemistry & Molecular Biology
Alice Vetrano, Francesco Gabriele, Nicoletta Spreti
Summary: Horse Liver Alcohol Dehydrogenase (HLADH) was immobilized on calcium-alginate beads for oxidation and reduction reactions. By adding calcium ions, the beads were able to maintain their structure and showed optimal performance at a concentration of 2 mM Ca2+. The immobilized enzyme demonstrated excellent reusability, storage stability, and thermal stability.
Article
Biochemistry & Molecular Biology
Mohamed A. A. Abdella, Samia A. Ahmed, Mohamed E. Hassan
Summary: Protease from Bacillus thuringiensis strain-MA8 was immobilized onto Alginate/dextrose beads with an immobilization yield of 77.6%. The immobilized protease showed higher thermal stability, lower activation energy, and a longer half-life compared to the free enzyme. The Alg/dex/protease also exhibited increased Km and Vmax values, indicating enhanced catalytic efficiency. Furthermore, it displayed good reusability after multiple hydrolysis cycles.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Karima Salem, Ylenia Jabalera, Jose David Puentes-Pardo, Jesus Vilchez-Garcia, Adel Sayari, Aida Hmida-Sayari, Concepcion Jimenez-Lopez, Massimiliano Perduca
Summary: Enzyme immobilization on magnetic nanoparticles offers reusability, stability, and robust activity under various conditions. In this study, microbial alpha-amylase and xylanase were successfully immobilized on different types of MNPs using optimized protocols, showing potential for bioindustry applications.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Applied
Luka Velickovic, Ana Simovic, Nikola Gligorijevic, Aurelien Thureau, Milica Obradovic, Tamara Vasovic, Georgios Sotiroudis, Maria Zoumpanioti, Annie Brulet, Tanja Cirkovic Velickovic, Sophie Combet, Milan Nikolic, Simeon Minic
Summary: This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). The purified R-PC showed high purity and maintained its native form and stability under specific conditions. The encapsulation of R-PC in calcium alginate beads demonstrated effective preservation of its color and antioxidant capacity.
Article
Biotechnology & Applied Microbiology
Sadia Noreen, Sofia Perveen, Nusrat Shafiq, Sadia Aslam, Hafiz M. N. Iqbal, Syed Salman Ashraf, Muhammad Bilal
Summary: Immobilization of laccase from Trametes versicolor onto functionalized graphene oxide resulted in FGO-TvL nanoassemblies, which exhibited improved enzyme stability and catalytic efficiency. The immobilized FGO-TvL showed great potential in decolorizing various textile industry effluents, highlighting its applicability in sustainable bioremediation applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biotechnology & Applied Microbiology
Shohreh Ariaeenejad, Elaheh Motamedi
Summary: This study successfully stabilized laccase by immobilizing it on sodium alginate-based hydrogels, allowing it to maintain high activity in acidic and alkaline conditions. The immobilized laccase also demonstrated high activity at high temperatures and could be recycled, showing good performance in enzymatic hydrolysis of rice straw with reduced phenol production.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Annemarie A. A. Lee, Esabelle D. D. Gervasio, Riley O. O. Hughes, Alexandra A. A. Maalouf, Samantha A. A. Musso, Alicia M. M. Crisalli, Elisa M. M. Woolridge
Summary: Xylanase can maintain high activity when encapsulated with alginate and exposed to the oxidative conditions. Cu2+-alginate provided better protection for xylanase than Ca2+-alginate. These findings are significant for biomass delignification.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Monica Jimenez-Carretero, Ylenia Jabalera, Alberto Sola-Leyva, Maria P. Carrasco-Jimenez, Concepcion Jimenez-Lopez
Summary: The immobilization of enzymes on magnetic nanoparticles has attracted interest in detecting contaminants in water samples due to their magnetic control, concentration, and reuse capabilities. In this study, nanoassemblies composed of inorganic or biomimetic magnetic nanoparticles were developed for the detection of trace amounts of organophosphate pesticides and antibiotics in water. Enzyme immobilization was achieved through electrostatic interaction or covalent bonds, with covalent bonding yielding better results. The optimized nanoassemblies demonstrated the ability to detect pollutants at low concentrations and provided enhanced stability and reuse of the enzymes.
Article
Chemistry, Physical
Mozhgan Razzaghi, Ahmad Homaei, Roohullah Hemmati, Dariush Saberi, Soudabeh Kavousipour
Summary: Cellulose nanocrystals/gold nanoparticle hybrids were synthesized using hydrothermal treatment without toxic chemicals. The immobilized enzyme showed increased optimal temperature and retained higher activity and efficiency under extreme conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Applied
Weerapha Panatdasirisuk, Kajornsak Faungnawakij, Verawat Champreda
Summary: The use of immobilized enzymes has gained attention in industrial bioprocesses due to their efficiency and environmental benefits. However, their practical applicability remains challenging due to factors such as material separation and resistance to stirring. In this study, a novel PAM bead was developed to support the enzyme, providing improved stability and reusability.
REACTIVE & FUNCTIONAL POLYMERS
(2023)
Article
Biotechnology & Applied Microbiology
Hanieh Mohammadi-Mahani, Arastoo Badoei-dalfard, Zahra Karami
Summary: In the study, a purified lipase from Enterobacter sp. MG10 was immobilized using different methods, with CGLNF showing broader activity, higher efficiency, significantly reduced K-m value towards pNPP substrate, and high efficiency in converting prosopis stephanina oil to biodiesel. The results indicate the high potential of CGLNF nanobiocatalyst in synthetic biotechnology, particularly in biodiesel production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Zainab Bibi, Hafsa Sattar, Muhammad Asif Nawaz, Asad Karim, Sidra Pervez, Shah Ali Ul Qader, Afsheen Aman
Summary: The current study successfully analyzed the biocompatibility and robust nature of solid macrogel beads composed of the polyacrylamide hydrogel matrix. The immobilized xylanase showed remarkable catalytic properties and stability compared to its soluble counterpart. The immobilized enzyme also exhibited good tolerance to high temperatures and recycling efficiency, making it a promising candidate for various industrial bioprocesses.
CURRENT RESEARCH IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shireen A. A. Saleh, Walaa A. Abdel Wahab, Faten A. Mostafa, Marwa I. Wahba
Summary: Amidated pectin-polyethylene imine-glutaraldehyde (AP-PEI-GA) immobilizer with high xylanase activity was prepared and the ideal protocol for its preparation was investigated. Covalent coupling to AP-PEI-GA improved the thermodynamic properties of Aspergillus niger xylanase.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Hao Zhu, Jinhang Chen, Yi Zhang, Kheng-Lim Goh, Chuyun Wan, Dongyun Zheng, Mingming Zheng
Summary: Novel organic-inorganic hybrid nanoflowers (AY-10@AXH-HNFs) were prepared by coordinating Cu2+ with both endopeptidase and exopeptidase. The AY-10@AXH-HNFs exhibited significantly higher catalytic activity and stability compared to free enzymes. This study introduces a promising approach for protein and food processing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Reda M. El-Shishtawy, Nahed S. E. Ahmed, Yaaser Q. Almulaiky
Summary: The composite material of chitosan/zinc oxide/iron oxide used for the immobilization of catalase exhibits good stability and high immobilization yield.
Article
Environmental Sciences
Tayyaba Asif, Urooj Javed, Syeda Bushra Zafar, Asma Ansari, Shah Ali Ul Qader, Afsheen Aman
WASTE AND BIOMASS VALORIZATION
(2020)
Article
Chemistry, Physical
Faiza Shahid, Asma Ansari, Afsheen Aman, Shah Ali Ul Qader
Article
Biochemistry & Molecular Biology
Fariha Ibrahim, Nadir Naveed Siddiqui, Afsheen Aman, Shah Ali Ul Qader, Asma Ansari
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Rabeeya Iftikhar, Asma Ansari, Nadir Naveed Siddiqui, Fayaz Hussain, Afsheen Aman
Summary: Fructan based biopolymers, specifically levan-type fructan, exhibit unconventional physical properties and biocompatibility, making them potential candidates for food and drug formulations.
CARBOHYDRATE RESEARCH
(2021)
Article
Biophysics
Asad Karim, Zainab Bibi, Haneef Ur Rehman, Afsheen Aman, Shah Ali Ul Qader, Muhammad Hamid Rashid
Summary: In this study, CMCase from Bacillus licheniformis KIBGE-IB2 was immobilized within agarose gel matrix, leading to improved catalytic properties with reduced maximum reaction rate and increased Michaelis-Menten constant. The immobilized enzyme showed enhanced stability and could be reused up to eight reaction cycles, indicating its potential application in various industrial processes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Environmental Sciences
Madiha Ansari, Urooj Zafar, Uroosa Ejaz, Muhammad Sohail, Aiman Pirzada, Afsheen Aman
Summary: The study focused on composting of chemically pretreated and fermented sugarcane bagasse, which showed significant degradation of cellulose and lignin. The compost resulted in high germination index and water holding capacity, with no phytotoxicity, indicating potential for establishing zero-waste sugarcane bagasse biorefinery.
JOURNAL OF MATERIAL CYCLES AND WASTE MANAGEMENT
(2021)
Article
Multidisciplinary Sciences
Sadaf Raza, Asma Ansari, Nadir Naveed Siddiqui, Fariha Ibrahim, Muhammad Ishaque Abro, Afsheen Aman
Summary: This study utilized a biologically mediated approach to synthesize silver nanoparticles and silver-chitosan nanocomposites, characterizing them through various spectroscopic analyses and demonstrating their promising antimicrobial potential. The silver-chitosan nanocomposites exhibited synergistic behavior against various bacterial species and showed no cytotoxic effects on NIH/3T3 cell lines.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Asad Karim, Zainab Bibi, Muhammad Asif Nawaz, Afsheen Aman, Shah Ali Ul Qader
Summary: The study analyzed the kinetic and thermodynamic parameters of free and immobilized CMCase, showing that immobilized enzyme had lower enthalpy of activation and irreversible inactivation energy compared to free enzyme. However, there were changes in Michaelis-Menten constant and maximum reaction rate after immobilization.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Afsheen Aman, Faiza Shahid, Sidra Pervez
Summary: The study successfully developed a chitosan-based hydrogel system crosslinked with glucosyltransferase, achieving functionalization of hydrogel beads and crosslinking with enzyme fragments. The improved catalytic performance of the modified crosslinked dextransucrase suggests its potential as a microreactor for catalysis of profitable macromolecules.
BIOTECHNOLOGY LETTERS
(2022)
Article
Food Science & Technology
Asma Ansari, Fariha Ibrahim, Muhammad Samee Haider, Afsheen Aman
Summary: The study demonstrates that Bac-IB45 produced by Lactiplantibacillus plantarum can be used as a biopreservative to effectively improve the shelf life of meat under refrigeration, with its unique N-terminal sequence aiding in inhibiting bacterial growth and preserving the texture and color of the meat.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Multidisciplinary Sciences
Syeda Bushra Zafar, Afsheen Aman
Summary: Statistical methods are important for optimizing fermentation practices and increasing productivity of biological metabolites. This study utilized a combinatorial approach of classical mutagenesis and statistical optimization to significantly improve the yield of dextransucrase, an important enzyme. The results demonstrate the potential for statistical approaches to enhance enzyme productivity and expand the applications of dextran.
JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE
(2022)
Article
Plant Sciences
Faisal Mehdi, Xinlong Liu, Zunaira Riaz, Urooj Javed, Afsheen Aman, Saddia Galani
Summary: This study investigated the impact of thermal stress on sucrose-metabolizing enzymes in sugarcane and found that sucrose content, enzyme expression, and sugar recovery rate varied at different developmental stages. Heat shock decreased the expression of sucrose metabolizing enzymes and sucrose content, while increasing the levels of antioxidant substances. One of the cultivars showed better adaptability under heat stress conditions. This research is important for understanding the acclimation of sugarcane to thermal stress and provides new directions for sugarcane molecular breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Zainab Bibi, Hafsa Sattar, Muhammad Asif Nawaz, Asad Karim, Sidra Pervez, Shah Ali Ul Qader, Afsheen Aman
Summary: The current study successfully analyzed the biocompatibility and robust nature of solid macrogel beads composed of the polyacrylamide hydrogel matrix. The immobilized xylanase showed remarkable catalytic properties and stability compared to its soluble counterpart. The immobilized enzyme also exhibited good tolerance to high temperatures and recycling efficiency, making it a promising candidate for various industrial bioprocesses.
CURRENT RESEARCH IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fatima Aslam, Asma Ansari, Afsheen Aman, Granaz Baloch, Gissed Nisar, Abdul Hameed Baloch, Haneef Ur Rehman
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Haneef Ur Rehman, Muhammad Asif Nawaz, Sidra Pervez, Muhsin Jamal, Mohammad Attaullah, Afsheen Aman, Shah Ali Ul Qader