期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 410, 期 14, 页码 3239-3252出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-018-0943-8
关键词
RNA; Sample preparation; In vitro transcription; Chemical synthesis; Preparative high-performance liquid chromatography; Structural biology
资金
- Karolinska Institutet [DNR 2-3707/2013]
- Vetenskapsradet [2014-4303]
- Stiftelsen for Strategisk Forskning [ICA14-0023]
- Jeansson Stiftelsen [JS2015-0126]
- Ragnar Soderbergs Stiftelse [M91-14]
- Swedish Foundation for Strategic Research (SSF) [ICA14-0023] Funding Source: Swedish Foundation for Strategic Research (SSF)
RNA is becoming more important as an increasing number of functions, both regulatory and enzymatic, are being discovered on a daily basis. As the RNA boom has just begun, most techniques are still in development and changes occur frequently. To understand RNA functions, revealing the structure of RNA is of utmost importance, which requires sample preparation. We review the latest methods to produce and purify a variation of RNA molecules for different purposes with the main focus on structural biology and biophysics. We present a guide aimed at identifying the most suitable method for your RNA and your biological question and highlighting the advantages of different methods.
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