Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Food Science & Technology
Cheng Li, Zhiqian Liu, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: Milk is a rich source of lipids, and LC-MS is the predominant technique for lipid identification and quantification. However, no standard methods are currently available for the quantification of milk phospholipids. This study aims to examine the factors influencing the quantification outcome of bovine milk phospholipids and discuss the pros and cons of current LC-MS methods.
Article
Chemistry, Analytical
Buyisile Mkhize, Tracy Kellermann, Jennifer Norman, Sandra Castel, Anton Joubert, Marthinus van der Merwe, Kelly E. Dooley, Jyoti S. Mathad, Lubbe Wiesner
Summary: A robust analytical method based on liquid chromatography coupled to tandem mass spectrometry was developed and validated for accurate quantification of rifapentine and 25-O-desacetyl rifapentine in human breast milk. The method showed reliable accuracy and precision, and demonstrated specificity for the targeted drugs.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Harald Schoeny, Evelyn Rampler, Dinh Binh Chu, Anna Schoeberl, Luis Galvez, Markus Blaukopf, Paul Kosma, Gunda Koellensperger
Summary: Standardization is crucial in lipidomics, with proposed traceable lipid class quantification for validation of workflows. Utilizing P-31 NMR and ICP-MS to validate LC-MS-based approaches, a novel quantification strategy based on lipid class separation and mass spectrometry is introduced. The concept proves interesting with low limits of detection and independent calibration strategies for quantitative assessment of lipid species.
ANALYTICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Jiahua Zhang, Ziman Fu, Weiwei Jiang, Yingying Zhang, Yiting Wang, Chunjian Shan
Summary: Breast milk jaundice (BMJ) is common in breastfed newborns, with researchers focusing on peptides in breast milk. By using LC-MS/MS analysis, this study identified 245 peptides in breast milk, with 30 differentially expressed peptides. GO analysis revealed that these peptides may play a role in BMJ pathogenesis.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2022)
Article
Chemistry, Analytical
C. Bottinelli, R. Nicoli, F. Bevalot, N. Cartiser, C. Roger, K. Chikh, T. Kuuranne, L. Fanton, J. Guitton
Summary: The study aimed to develop the first validated method for analyzing human insulin and its synthetic analogues, primarily for plasma and post-mortem sera. Different extraction processes were evaluated for the lowest limit of detection for target analogues, and the limits of quantification were validated in plasma and post-mortem sera. Results showed that at the LOQ level, accuracy was between 70 and 130%, with precision lower than 30%.
Article
Biochemistry & Molecular Biology
Zhiqian Liu, Simone Rochfort
Summary: Regioisomers of triacylglycerols in milk have differential effects on human absorption, but quantifying their ratios remains challenging due to the lack of easy chromatographic separation techniques. This study confirmed the feasibility of using fragment ion intensity ratio to determine regioisomer pairs and demonstrated the ability of C30 stationary phase in resolving interfering isomers. The research proposed a novel algorithm to consider double bond isomers and eliminate interference from isobaric ions, presenting a simple LC-MS method suitable for genetic analysis of milk samples.
Article
Biochemical Research Methods
Jernej Oberckal, Humna Liaqat, Bojana Bogovic Matijasic, Vita Rozman, Primoz Treven
Summary: This study aimed to validate an efficient method for the detection and quantification of human lactoferrin (hLF) in human milk. The results showed that cation-exchange chromatography on CIM (R) monolithic columns was accurate and efficient for this purpose. The concentration of hLF in human milk was not significantly affected by the sample collection method, but was negatively correlated with the stage of lactation.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Nutrition & Dietetics
Ikuya Shirai, Yu Iwasaki, Koji Karasawa, Yasutaka Shigemura, Shigeru Katayama
Summary: Agaro-oligosaccharides (AOSs) have biological activities in peripheral tissues, and AOSs with low-molecular weight can permeate the gastrointestinal tract. The systemic absorption of agarobiose is better than that of agarotetraose.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
Jensen Cherewyk, Taylor Grusie-Ogilvie, Barry Blakley, Ahmad Al-Dissi
Summary: This study validated a new method using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) to quantify 12 epimers of ergot alkaloids in wheat. The method showed low LOD and LOQ values, allowing for sensitive detection of ergot epimers. The study also found differences between the R and S-epimers, which has rarely been documented in previous research.
Article
Environmental Sciences
Dorothea F. K. Rawn, Guy Dufresne, Genevieve Clement, William D. Fraser, Tye E. Arbuckle
Summary: The MIREC study in Canada found that perfluorinated alkyl substances (PFAS) are commonly present in breast milk, and their concentrations are inversely correlated with age. Primiparous women had higher PFAS concentrations in milk, while the region of maternal birth did not affect PFAS concentrations. Additionally, PFAS with long carbon chains did not exceed detection limits in Canadian breast milk samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Applied
Rui Li, Yalin Zhou, Yajun Xu
Summary: In this study, UPLC-QE-HF-MS was used to detect human milk oligosaccharides, bovine milk oligosaccharides, goat milk oligosaccharides, and rat milk oligosaccharides. The four milk oligosaccharides showed significant differences in number and composition. Rat milk oligosaccharides were found to contain 14 neutral and 3 acidic oligosaccharides for the first time. The composition and abundances of rat milk oligosaccharides were more similar to human milk oligosaccharides, suggesting their potential use as models in biological/biomedical studies. Bovine milk oligosaccharides and goat milk oligosaccharides could have applications in medical and functional foods as promising bioactive molecules.
Article
Biochemical Research Methods
Anastasia Pafili, Thomas Meikopoulos, Eleni Kontogiannidou, Spyros Papageorgiou, Efterpi Demiri, Despoina Meimari, Dimitrios G. Fatouros, Helen Gika, Georgios Theodoridis
Summary: A novel HPLC-MS/MS method was developed for the simultaneous determination of four UV filters in the in vitro skin permeation process. The method was validated for detection limits, linearity range, and precision, providing satisfactory results. Analysis of target compounds in samples after permeability studies showed low concentrations in the acceptor phase, indicating product safety.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Jan Thomann, Laura Ley, Aaron Klaiber, Matthias E. Liechti, Urs Duthaler
Summary: In this study, a bioanalytical method was developed for the rapid quantification of mescaline and its metabolites in human plasma. The method showed reliability and ease of use, making it suitable for forensic applications as well as investigating the clinical pharmacokinetics of mescaline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Nutrition & Dietetics
Isabel Ten-Domenech, Mari Merce Cascant-Vilaplana, Victor Navarro-Esteve, Birgit Felderer, Alba Moreno-Gimenez, Ivan Rienda, Maria Gormaz, Marta Moreno-Torres, David Perez-Guaita, Guillermo Quintas, Julia Kuligowski
Summary: This preliminary study aimed to characterize the cellular metabolome of human milk over the course of lactation. Cells were isolated and characterized, and their metabolites were analyzed using mass spectrometry. The results showed significant variations in the cellular composition and phenotypic properties of human milk during lactation. This study provides a basis for future investigations on the metabolomic fraction of the cellular compartment of human milk.
Article
Nutrition & Dietetics
Sean Austin, Carlos A. De Castro, Thierry Benet, Yangfeng Hou, Henan Sun, Sagar K. Thakkar, Gerard Vinyes-Pares, Yumei Zhang, Peiyu Wang
Article
Chemistry, Applied
Sathaporn Srichuwong, Delphine Curti, Sean Austin, Roberto King, Lisa Lamothe, Hugo Gloria-Hernandez
Letter
Biochemical Research Methods
S. Austin, V. Spichtig
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2012)
Article
Biochemical Research Methods
Veronique Spichtig, Julien Michaud, Sean Austin
ANALYTICAL BIOCHEMISTRY
(2010)
Article
Biochemical Research Methods
Thierry Benet, Sean Austin
ANALYTICAL BIOCHEMISTRY
(2011)
Article
Multidisciplinary Sciences
Shirin Macias Rostami, Thierry Benet, Julie Spears, Arleigh Reynolds, Ebenezer Satyaraj, Norbert Sprenger, Sean Austin
Article
Chemistry, Analytical
Sean Austin, Thierry Benet, Julien Michaud, Denis Cuany, Philippe Rohfritsch
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
(2014)
Article
Obstetrics & Gynecology
Francesca Giuffrida, Sean Austin, Denis Cuany, Belen Sanchez-Bridge, Karin Longet, Emmanuelle Bertschy, Julien Sauser, Sagar K. Thakkar, Le Ye Lee, Michael Affolter
JOURNAL OF PERINATOLOGY
(2019)
Article
Nutrition & Dietetics
Sean Austin, Carlos A. De Castro, Norbert Sprenger, Aristea Binia, Michael Affolter, Clara L. Garcia-Rodenas, Lydie Beauport, Jean-Francois Tolsa, Celine J. Fischer Fumeaux
Article
Chemistry, Analytical
Veronique Spichtig, Sean Austin, Kommer Brunt, Jeroen Van Soest, Peter Sanders
JOURNAL OF AOAC INTERNATIONAL
(2020)
Article
Nutrition & Dietetics
Peter Duncan, Olli Aitio, Annamari Heiskanen, Ritva Niemela, Juhani Saarinen, Jari Helin, Nadine Porta, Muriel Fiaux, Denis Moennoz, Mireille Golliard, Christine Cherbut, Rafael Berrocal, Sean Austin, Norbert Sprenger
Review
Nutrition & Dietetics
Norbert Sprenger, Hanne L. P. Tytgat, Aristea Binia, Sean Austin, Atul Singhal
Summary: Human milk oligosaccharides (HMOs) are important components of breast milk that have significant impact on the health and nutrition of infants. The research on HMOs has flourished in the past decade, thanks to advancements in biotechnology that allow for large-scale production of genuine HMOs. The clinical relevance of specific HMOs has been supported by clinical intervention trials in formula-fed infants, as well as clinical observations and animal models.
JOURNAL OF HUMAN NUTRITION AND DIETETICS
(2022)
Article
Nutrition & Dietetics
Jean-Christophe Roze, Mickael Hartweg, Laure Simon, Helene Billard, Yipu Chen, Sean Austin, Cecile Boscher, Thomas Moyon, Dominique Darmaun, Clara L. Garcia Rodenas, Clair-Yves Boquien
Summary: This study explored the impact of HMOs on growth and neurodevelopment in preterm infants. The findings showed that HMO concentrations decreased with advancing postnatal age. Total HMOs were positively correlated with neonatal length growth. Among Secretor(+) Lewis(+) mothers, LNFP-III was significantly associated with neurodevelopmental scores at 2 years.
CLINICAL NUTRITION
(2022)
Article
Chemistry, Analytical
Denis Cuany, Fikrey Andetsion, Xavier Fontannaz, Thierry Benet, Veronique Spichtig, Sean Austin
Summary: A method for determining GOS in infant formula and adult nutritionals was developed and validated through single laboratory validation. The method involves labeling reducing oligosaccharides with 2AB, separating them by hydrophilic interaction LC, and assuming equimolar response in the detector. The method proved to be suitable for accurate determination of GOS content in products with a high lactose background.
JOURNAL OF AOAC INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)