Article
Obstetrics & Gynecology
Huijuan Ruan, Qingya Tang, Yajie Zhang, Xuelin Zhao, Yi Xiang, Yi Feng, Wei Cai
Summary: This study compared the results of a mid-infrared analyzer and an ultrasound-based milk analyzer for macronutrients in breast milk and unified the results using machine learning. The results showed significant differences between the two methods, but after data adjustments, they could be considered comparable.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Article
Agriculture, Dairy & Animal Science
M. Portnoy, C. Coon, D. M. Barbano
Summary: The study aimed to redesign milk calibration samples, determine within-and between laboratory variation, and optimize PLS models for predicting MUN concentration.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Nutrition & Dietetics
Manuela Cardoso, Daniel Virella, Ana Luisa Papoila, Marta Alves, Israel Macedo, Diana Silva, Luis Pereira-da-Silva
Summary: This study compares fortification of human milk based on measured macronutrient content versus assumed content, and finds that fortification based on measured content improves nutrition support, growth, and body composition in preterm infants.
Article
Nutrition & Dietetics
Morgan B. Pyles, Kristin Brock, Rachel R. Schendel, Laurie M. Lawrence
Summary: Accurately determining the macronutrient profile of mare milk is essential for studying foals' growth and development. This study optimized an extraction method for mare milk oligosaccharides and compared the use of FTIR spectroscopy to wet chemistry methods for analyzing protein, fat, and lactose. The optimized method achieved good recovery rates for milk oligosaccharides, while FTIR analysis showed equivalent protein contents to wet chemistry methods and underestimated fat content. FTIR also overestimated lactose content by including milk oligosaccharides.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
Octave S. Christophe, Clement Grelet, Carlo Bertozzi, Didier Veselko, Christophe Lecomte, Peter Hoeckels, Andreas Werner, Franz-Josef Auer, Nicolas Gengler, Frederic Dehareng, Helene Soyeurt
Summary: This study successfully developed and validated robust multi-breed and multi-country models predicting major minerals in milk through milk mid-infrared spectrometry, showing the importance of covering spectral variability of a country before making predictions.
Article
Agriculture, Dairy & Animal Science
M. Frizzarin, T. F. O'Callaghan, T. B. Murphy, D. Hennessy, A. Casa
Summary: The prevalence of grass-fed labeled food products has increased, making verification of these claims crucial for consumer confidence. Mid-infrared spectroscopy is effective for authenticating milk sources, with linear discriminant analysis and partial least squares discriminant analysis offering the highest accuracy in predicting cow diet based on spectra. Efficient strategies for selecting discriminating wavelengths in the spectra are also emphasized.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
M. Portnoy, C. Coon, D. M. Barbano
Summary: The enzymatic spectrophotometric method for milk urea nitrogen determination showed good within and between laboratory performance, outperforming other methods such as the differential pH MUN method. Collaborative studies are needed to achieve official method status from international organizations.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Food Science & Technology
Carlotta Ceniti, Anna Antonella Spina, Cristian Piras, Francesca Oppedisano, Bruno Tilocca, Paola Roncada, Domenico Britti, Valeria Maria Morittu
Summary: To ensure the safety and quality of milk, new tools have been developed to detect chemical contaminants, toxins, veterinary drugs, and adulteration of milk from different species. Mid-infrared spectroscopy and Fourier-transform infrared have been commonly used technologies for rapid screening of hazardous substances and confirmation of milk authenticity. These fingerprint methods can effectively determine the characteristics of raw materials without knowing the identity of each constituent, making them potential screening methods for adulteration detection. This paper reviews the latest advances in applying mid-infrared spectroscopy for the detection and quantification of adulterants, milk dilution, pathogenic bacteria, veterinary drugs, and hazardous substances in milk.
Article
Agriculture, Dairy & Animal Science
Kathryn M. Tiplady, Thomas J. Lopdell, Richard G. Sherlock, Thomas J. J. Johnson, Richard J. Spelman, Bevin L. Harris, Stephen R. Davis, Mathew D. Littlejohn, Dorian J. Garrick
Summary: This study compares the genetic characteristics of FT-MIR predicted fatty acids and individual milk proteins with those measured directly, and finds that genetic correlations are typically high, but phenotypic variations may be influenced by different genetic architectures.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Obstetrics & Gynecology
Chiara Tabasso, Pasqua Piemontese, Nicola Pesenti, Michela Perrone, Camilla Menis, Nadia Liotto, Orsola Amato, Anna Orsi, Domenica Mallardi, Fabio Mosca, Paola Roggero
Summary: This study compared the impact of random pooling and target pooling on the macronutrient content of donor human milk for preterm infants, and identified the best random pooling strategy to achieve a similar macronutrient composition as target pooling. The results showed that when target pooling is not feasible, a random pooling strategy with at least five donors should be used to obtain a better macronutrient content.
BREASTFEEDING MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Brenda Contla Hernandez, Nicolas Lopez-Villalobos, Matthieu Vignes
Summary: This study utilized various machine learning techniques to identify cow sickness and demonstrated that neural networks can identify health problems with a reasonable level of accuracy. Early detection of health problems in dairy cattle is crucial for reducing economic losses.
Article
Nursing
Rachael L. Lamb, Jillian J. Haszard, Helen M. J. Little, Anthea F. Franks, Maggie G. Meeks
Summary: This study analyzed the macronutrient characteristics and energy content of human milk donated to a milk bank in New Zealand, as well as the impact of Holder pasteurization on these nutrients. The results showed variations in energy, fat, protein, and carbohydrate content between preterm and term milk, with no significant effect of pasteurization on these values.
JOURNAL OF HUMAN LACTATION
(2021)
Article
Nutrition & Dietetics
Stephanie Fusch, Gerhard Fusch, Efrah Yousuf, Markus Rochow, Hon Yiu So, Christoph Fusch, Niels Rochow
Summary: This study analyzed the macronutrient composition of different commercially available fortifiers and the effect of different fortification strategies on nutritional intake of preterm infants. Standard fortification, breast milk batching, and partial lyophilization of human milk resulted in deficient and imbalanced enteral intakes in a significant proportion of infants. Target fortification reduced this variation in a higher percentage of samples.
FRONTIERS IN NUTRITION
(2021)
Article
Multidisciplinary Sciences
Gabriela E. Leghi, Ching T. Lai, Ardra Narayanan, Merryn J. Netting, Michael Dymock, Alethea Rea, Mary E. Wlodek, Donna T. Geddes, Beverly S. Muhlhausler
Summary: The study found that concentrations of fat, protein, and lactose in mature human milk did not significantly differ based on time of day, day of week, or which breast was used for collection, over a 3-week period.
SCIENTIFIC REPORTS
(2021)
Review
Food Science & Technology
Carlos Franco, Cristina Fente, Cristina Sanchez, Alexandre Lamas, Alberto Cepeda, Rosaura Leis, Patricia Regal
Summary: The functionality of breast milk in terms of immunity is well-known, however, many breastfed infants exhibit sensitization to potential allergens and clinical reactivity before the introduction of complementary feeding. The potential induction of early oral tolerance through allergen exposure in breast milk remains controversial. This scoping review critically appraised the content of cow's milk antigens in human milk to better understand their impact on allergic disease development.
Article
Agriculture, Dairy & Animal Science
C. Fuerer, R. Jenni, L. Cardinaux, F. Andetsion, S. Wagniere, J. Moulin, M. Affolter
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Nutrition & Dietetics
Magalie Sabatier, Clara L. Garcia-Rodenas, Carlos A. De Castro, Peter Kastenmayer, Mario Vigo, Stephane Dubascoux, Daniel Andrey, Marine Nicolas, Janique Richoz Payot, Valentine Bordier, Sagar K. Thakkar, Lydie Beauport, Jean-Francois Tolsa, Celine J. Fischer Fumeaux, Michael Affolter
Review
Biochemical Research Methods
Loic Dayon, Michael Affolter
EXPERT REVIEW OF PROTEOMICS
(2020)
Article
Nutrition & Dietetics
Tinu Mary Samuel, Carlos Antonio De Castro, Stephane Dubascoux, Michael Affolter, Francesca Giuffrida, Claude Billeaud, Jean-Charles Picaud, Massimo Agosti, Isam Al-Jashi, Almerinda Barroso Pereira, Maria Jose Costeira, Maria Gorett Silva, Giovanna Marchini, Thameur Rakza, Kirsti Haaland, Tom Stiris, Silvia-Maria Stoicescu, Cecilia Martinez-Costa, Mireilla Vanpee, Magnus Domellof, Euridice Castaneda-Gutierrez, Sagar Kiran Thakkar, Irma Silva-Zolezzi
Article
Chemistry, Analytical
Veronique Spichtig, Sean Austin, Kommer Brunt, Jeroen Van Soest, Peter Sanders
JOURNAL OF AOAC INTERNATIONAL
(2020)
Article
Nutrition & Dietetics
Peter Duncan, Olli Aitio, Annamari Heiskanen, Ritva Niemela, Juhani Saarinen, Jari Helin, Nadine Porta, Muriel Fiaux, Denis Moennoz, Mireille Golliard, Christine Cherbut, Rafael Berrocal, Sean Austin, Norbert Sprenger
Article
Nutrition & Dietetics
Veronica Caldeo, Eimear Downey, Carol-Anne O'shea, Michael Affolter, Sheri Volger, Marie-Claude Courtet-Compondu, Carlos Antonio De Castros, James A. O'Mahony, C. Anthony Ryan, Alan L. Kelly
Summary: This study evaluated the composition, protein profile, and plasmin activity of human milk from mothers who delivered infants at different gestational ages. Results showed that protein content and levels of some individual proteins were significantly affected by gestational age at birth and duration of lactation, with differences in proteolytic activity in milk samples. Compositional differences between term and pre-term infant milk were most significant in terms of protein profile.
CLINICAL NUTRITION
(2021)
Article
Biochemical Research Methods
Loic Dayon, Charlotte Macron, Sabine Lahrichi, Antonio Nunez Galindo, Michael Affolter
Summary: A highly automated proteomic workflow for human milk sample analysis was developed, utilizing liquid chromatography mass spectrometry. Different workflows were evaluated and optimized, with the aim of allowing analysis of large sample cohorts in clinical research. This efficient workflow provides a new approach to studying the proteome of human milk.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Nutrition & Dietetics
Tristan Bourdeau, Michael Affolter, Lenaick Dupuis, Alexandre Panchaud, Sabine Lahrichi, Loraine Merminod, Christine Martin-Paschoud, Rachel Adams, Sophie Nutten, Carine Blanchard
Summary: Human clinical trials have demonstrated that a specific partially hydrolyzed 100% whey-based infant formula can reduce atopic dermatitis risk in the first year of life. However, differences in peptide composition and functional properties were found among different formulas, indicating that not all formulas have the same ability to reduce allergenicity and induce oral tolerance.
Review
Nutrition & Dietetics
Norbert Sprenger, Hanne L. P. Tytgat, Aristea Binia, Sean Austin, Atul Singhal
Summary: Human milk oligosaccharides (HMOs) are important components of breast milk that have significant impact on the health and nutrition of infants. The research on HMOs has flourished in the past decade, thanks to advancements in biotechnology that allow for large-scale production of genuine HMOs. The clinical relevance of specific HMOs has been supported by clinical intervention trials in formula-fed infants, as well as clinical observations and animal models.
JOURNAL OF HUMAN NUTRITION AND DIETETICS
(2022)
Review
Biochemical Research Methods
Loic Dayon, Ornella Cominetti, Michael Affolter
Summary: Biological fluids, such as blood and cerebrospinal fluid, are important samples for diagnostic testing and monitoring. Proteomic technologies have been used to identify protein biomarkers in these fluids, but with limited success. Recent advancements in technology and methodologies have allowed for better analysis of human biological fluids, and this review focuses on recent highlights in the field of proteomics using mass spectrometry. Despite being overshadowed by genomics, proteomic tools and methodologies have matured and are now better suited for discovering innovative and robust biofluid biomarkers.
EXPERT REVIEW OF PROTEOMICS
(2022)
Article
Agriculture, Dairy & Animal Science
Holger A. Ebhardt, Pierre Ponchon, Konstantinos Theodosiadis, Christophe Fuerer, Marie -Claude Courtet-Compondu, Jonathan O'Regan, Michael Affolter, Yousef Joubran
Summary: High resolution mass spectrometry can identify hundreds to thousands of proteins per sample with label-free quantification, but the gold standard for quantitative proteomics is MRM. The authors demonstrate a reductionist approach using correlation analysis of dairy ingredients, successfully explaining variance in proteomics data with only 9 proteins. Their method, Trinity-MRM, shows reproducibility below 5% in independent experiments with double cream.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Nutrition & Dietetics
Jean-Christophe Roze, Mickael Hartweg, Laure Simon, Helene Billard, Yipu Chen, Sean Austin, Cecile Boscher, Thomas Moyon, Dominique Darmaun, Clara L. Garcia Rodenas, Clair-Yves Boquien
Summary: This study explored the impact of HMOs on growth and neurodevelopment in preterm infants. The findings showed that HMO concentrations decreased with advancing postnatal age. Total HMOs were positively correlated with neonatal length growth. Among Secretor(+) Lewis(+) mothers, LNFP-III was significantly associated with neurodevelopmental scores at 2 years.
CLINICAL NUTRITION
(2022)
Article
Chemistry, Analytical
Denis Cuany, Fikrey Andetsion, Xavier Fontannaz, Thierry Benet, Veronique Spichtig, Sean Austin
Summary: A method for determining GOS in infant formula and adult nutritionals was developed and validated through single laboratory validation. The method involves labeling reducing oligosaccharides with 2AB, separating them by hydrophilic interaction LC, and assuming equimolar response in the detector. The method proved to be suitable for accurate determination of GOS content in products with a high lactose background.
JOURNAL OF AOAC INTERNATIONAL
(2022)