Review
Biochemistry & Molecular Biology
Ladie Kimberly de la Cruz, Xingyue Ji, Xiaoxiao Yang, Binghe Wang
Summary: The article discusses recent advancements in utilizing prodrug approaches for the generation of carbon monoxide, including methods for CO release and formation of fluorescent reporters, and the construction of CO delivery platforms with additional functionalities. These approaches provide new insights for precise CO delivery and assessment of therapeutic efficacy.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Katharina Foremny, Wiebke S. Konerding, Ailke Behrens, Peter Baumhoff, Ulrich P. Froriep, Andrej Kral, Theodor Doll
Summary: The study explored the use of CNT-coated electrodes for neural interfacing, showing promising results but no improvements during acute recordings.
Article
Chemistry, Physical
Wen-Jie Shu, Zhe Ma, Xiaojie Tian, Fu Wang
Summary: In this study, a genetic switch system based on OATP1B3 is designed and optimized to achieve near-infrared fluorescent imaging of miRNA by the uptake of ICG dye. The system is shown to be sensitive for imaging miRNA in mammalian cells and functional for real-time imaging in living mice. This study establishes a novel genetic encoded reporter for near-infrared fluorescence imaging of miRNA.
Article
Engineering, Biomedical
Chuangui Sheng, Jian Zhao, Zhenghan Di, Yuanyu Huang, Yuliang Zhao, Lele Li
Summary: The in vivo optical imaging of inflammation-associated messenger RNAs (mRNAs) is challenging due to the non-specific signal amplification in healthy tissues. In this study, researchers designed a molecular beacon with apurinic/apyrimidinic sites, which could be activated and amplified by human apurinic/apyrimidinic endonuclease 1 specifically in inflammatory cells translocating from the nucleus to the cytoplasm. The sensitivity and tissue specificity of this engineered molecular beacon targeting interleukin-6 (IL-6) mRNA were evaluated in live mice by detecting acute inflammation in paws and drug-induced inflammation in livers. This enzymatic-amplification strategy has the potential for specific and sensitive imaging of other disease-relevant RNAs in vivo.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Federica Pisaneschi, Seth T. Gammon, Vincenzo Paolillo, Sarah A. Qureshy, David Piwnica-Worms
Summary: This study introduces a novel redox-tuned radiopharmaceutical called [F-18]4FN, which selectively binds proteins and cells oxidized by products of human MPO and H2O2, and can be detected using PET/CT technology. It shows promise as a robust reporter of activated innate immunity and has been successfully used to detect inflammation in mouse models.
NATURE BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lijuan Ye, Weibin Chen, Yuan Chen, Yuqin Qiu, Jun Yi, Xiaofang Li, Qiuxiao Lin, Bohong Guo
Summary: A hybrid nanocarrier was developed to facilitate transdermal delivery of drugs. The f-MWCNTs-KP-ES preparation showed sustained release effect and faster skin penetration rate compared to commercial gel. Fluorescence microscopy and in vivo pharmacokinetic experiments confirmed the faster and deeper penetration of f-MWCNTs-RB-ES. The overall data demonstrate the promising transdermal drug delivery system of f-MWCNTs composite ethosomes formulation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Shiyi Wang, Ziwen Zong, Jianguo Xu, Bangben Yao, Zhou Xu, Li Yao, Wei Chen
Summary: In this study, a visual strategy for rapid and ultrasensitive detection of OTA was proposed by integrating primer-mediated exponential rolling circle amplification (P-ERCA) with a designed nucleic acid lateral flow strip (LFS). This method demonstrates highly sensitive detection of OTA and could be utilized as a universal method for detection of other chemical analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Adi Hendler-Neumark, Verena Wulf, Gili Bisker
Summary: MicroRNAs (miRNAs) play important roles in cellular and biological processes. This study demonstrates a selection process for miRNA biomarkers using fluorescent single-walled carbon nanotube (SWCNT) sensors. One selected sensor showed high specificity and selectivity towards the target sequence in serum.
Article
Chemistry, Medicinal
Zhen Song, Qiang-Yan Zhang, Jia-Jing Li, Jing-Lian Su, Yuan-Hua Liu, Gong-Jun Yang, Huai-Song Wang
Summary: Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was used to initiate the detection of let-7a, followed by analysis using lateral flow assay (LFA) and electrochemical impedance spectroscopy.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Elisabeth Grimm, Franciscus van der Hoeven, Donato Sardella, Katrin Willig, Ulrike Engel, Nisha Veits, Robert Engel, Elisabetta Ada Cavalcanti-Adam, Felix Bestvater, Luca Bordoni, Richard Jennemann, Kai Schoenig, Ina Maria Schiessl, Roger Sandhoff
Summary: Clathrin-mediated endocytosis is a well-studied cellular uptake pathway, but the dynamic cooperation of its components in vivo is still not well understood. This study generated a reporter mouse model to track endocytosis by fusing eGFP to clathrin light chain a. The fusion protein was expressed in all tissues and could be visualized using fluorescence microscopy. The functionality of the reporter was confirmed by recruitment to nanobeads recorded by TIRF microscopy.
Article
Virology
Enyue Fang, Xiaohui Liu, Miao Li, Jingjing Liu, Zelun Zhang, Xinyu Liu, Xingxing Li, Wenjuan Li, Qinhua Peng, Yongxin Yu, Yuhua Li
Summary: Since its first isolation in 1943, the dengue virus (DENV) has spread globally, but effective antiviral drugs or vaccines are still lacking. Researchers constructed a reporter DENV containing the NanoLuc reporter gene, providing a stable tool for vaccine development and antiviral drug screening. Through a live imaging mouse model, the neurovirulence of the reporter virus was confirmed, and the importance of intestinal lymphoid tissues in DENV pathogenesis was revealed.
Article
Chemistry, Multidisciplinary
Yi Han, Shaoqiang Dong, Jiawei Shao, Wei Fan, Chunyan Chi
Summary: The successful synthesis of a carbon nanobelt (CNB) and the characterization of its unique structure and properties were reported in this study. The findings provide a new strategy for the synthesis of highly strained CNBs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Jongwan Choi, Jiwon Min, Jason Sahngwook Kim, Jung Hyun Park, Suwyoung Ly
Summary: This study compared the performance of a bismuth immobilized carbon nanotube paste electrode (BCE) with a carbon nanotube paste electrode (CE) for detecting epinephrine (EP). The BCE was found to be more efficient. The optimized conditions, including 0.3 V square-wave stripping voltammetry, 400 Hz frequency, -0.8 V initial potential, and 0.015 V increment potential, were applied to analyze a carp's front fin. The results showed that the BCE had a miniscule working electrode and a very low detection limit, and successfully analyzed the in vivo muscle's chronoamperometric nerve currents. These findings have potential applications in medical diagnostics, pharmaceuticals, interface controllers, and other fields.
Review
Biotechnology & Applied Microbiology
Tarana Siddika, Ilka U. Heinemann
Summary: MiRNAs are small non-coding RNAs that regulate posttranscriptional gene expression by interacting with target mRNAs. The finely controlled biogenesis, target recognition, and degradation of miRNAs play crucial roles in maintaining cell proliferation, growth, differentiation, and apoptosis. MiRNAs have emerged as potential biomarkers for disease diagnosis, with their expression and activity varying in different contexts such as developmental stages, tissues, and responses to external stimuli.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biophysics
Shah Zada, Huiting Lu, Wenhao Dai, Songsong Tang, Sikandar Khan, Fan Yang, Yuchun Qiao, Pengcheng Fu, Haifeng Dong, Xueji Zhang
Summary: The study developed an amplified imaging platform for intracellular miRNAs based on Mo2B nanosheets, which combined fluorescence quenching and hybridization chain reaction technologies for sensitive detection and imaging of miRNAs. This platform demonstrated excellent performance in distinguishing cancer cells from normal cells and monitoring miRNA expression changes in cancer cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Prakrit Jena, Mitchell Gravely, Christian Cupo, Mohammad Moein Safaee, Daniel Roxbury, Daniel A. Heller
Summary: This study proposes a method to improve quantification of nanomaterials at the single-cell and organelle level using the distinct optical properties of a heterogeneous nanomaterial preparation. The method, called hyperspectral counting, utilizes diffraction-limited imaging via hyperspectral microscopy and mathematical modeling to accurately estimate particle number counts in live cells and subcellular structures. The researchers applied this method to study the uptake of single-walled carbon nanotubes into cells and identified a higher rate of uptake compared to conventional counting methods. The study reveals significant heterogeneity and non-Gaussian distribution of nanotube uptake within cells, providing insights into cellular and subcellular nanoparticle concentrations.
Review
Pharmacology & Pharmacy
Chen Chen, Zvi Yaari, Elana Apfelbaum, Piotr Grodzinski, Yosi Shamay, Daniel A. Heller
Summary: Nanomedicine design is a trial-and-error process, and data science is becoming increasingly important in the field. Advanced data analytics, including machine learning, can predict nanomaterial synthesis and biological behaviors. Currently, data curation and data analytics in nanomedicine are proceeding independently, with potential opportunities for coordination.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Urology & Nephrology
Xiaojia Guo, Leyuan Xu, Heino Velazquez, Tian-Min Chen, Ryan M. Williams, Daniel A. Heller, Barbara Burtness, Robert Safirstein, Gary Desir
Summary: Kidney-targeted delivery of RNLS can prevent cisplatin-induced CKD by decreasing cell death and improving the viability of the renal proximal tubule.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Pharmacology & Pharmacy
Ryan M. Williams, Janki Shah, Elizabeth Mercer, Helen S. Tian, Vanessa Thompson, Justin M. Cheung, Madeline Dorso, Jaclyn M. Kubala, Lorraine J. Gudas, Elisa de Stanchina, Edgar A. Jaimes, Daniel A. Heller
Summary: The use of kidney-selective mesoscale nanoparticles (MNP) to deliver the reactive oxygen species scavenger edaravone improved renal function and reduced tubular epithelial cell damage and death in a mouse model of cisplatin-induced acute kidney injury (CI-AKI).
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Mitchell Gravely, Aidan Kindopp, Lauren Hubert, Matthew Card, Aceer Nadeem, Christopher Miller, Daniel Roxbury
Summary: The non-covalent biomolecular functionalization of fluorescent single-walled carbon nanotubes (SWCNTs) has led to their extensive use in in vitro and in vivo sensing and imaging applications. However, it has been observed that these SWCNTs tend to aggregate in protein-containing environments before interacting with cells at the nanobiointerface. This study shows that initially aggregated SWCNTs do not further accumulate within subcellular locations and that SWCNTs with a low degree of aggregation can induce significant deleterious effects on cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Merav Antman-Passig, Eitan Wong, Georgia R. Frost, Christian Cupo, Janki Shah, Albert Agustinus, Ziyu Chen, Chiara Mancinelli, Maikel Kamel, Thomas Li, Lauren A. Jonas, Yue-Ming Li, Daniel A. Heller
Summary: The researchers developed a near-infrared optical nanosensor that can detect the presence of A beta in live cells and mice. The sensor selectively responds to A beta through solvatochromic modulation, allowing for tracking of A beta accumulation. This technology provides insight into the molecular mechanisms underlying A beta neurotoxicity in the development of Alzheimer's disease.
Article
Cardiac & Cardiovascular Systems
Lance N. Benson, Yunmeng Liu, Xiangting Wang, Yunzhao Xiong, Sung W. Rhee, Yunping Guo, Katherine S. Deck, Christoph J. Mora, Lin-Xi Li, Lu Huang, J. Tucker Andrews, Zhiqiang Qin, Robert S. Hoover, Benjamin Ko, Ryan M. Williams, Daniel A. Heller, Edgar A. Jaimes, Shengyu Mu
Summary: This study investigated the interaction between CD8T cells and DCTs and its impact on hypertension. The results showed that activated CD8T cells enhance the interaction with DCTs through the upregulation of MHC-I and PDL1 on DCTs via IFN-gamma, leading to increased NCC expression in DCTs, excessive salt retention, and persistent elevation of blood pressure. Eliminating IFN-gamma or specific knockdown of PDL1 on DCTs prevented T cell homing into the kidney, thereby attenuating hypertension.
CIRCULATION RESEARCH
(2022)
Article
Engineering, Biomedical
Mijin Kim, Chen Chen, Peng Wang, Joseph J. Mulvey, Yoona Yang, Christopher Wun, Merav Antman-Passig, Hong-Bin Luo, Sun Cho, Kara Long-Roche, Lakshmi Ramanathan, Anand Jagota, Ming Zheng, YuHuang Wang, Daniel A. Heller
Summary: Ovarian cancer can be accurately predicted using a disease fingerprint obtained through machine learning from the spectra of near-infrared fluorescence emissions of a carbon nanotube sensor array.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Ron Feiner, Eleanor Johns, Merav Antman-Passig, Takeshi Irie, Naxhije Berisha, Hadas Oved, Doha Khan, Lukasz Witek, Richard M. White, Daniel A. Heller
Summary: Rubber band ligation is a commonly used method for treating internal hemorrhoids. However, it often causes pain and discomfort. To alleviate these side effects, researchers developed drug-eluting rubber bands that both maintain mechanical properties and release drugs effectively.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Oncology
Jaclyn M. Kubala, Kristian B. Laursen, Ryan Schreiner, Ryan M. Williams, Johannes C. van der Mijn, Michael J. Crowley, Nigel P. Mongan, David M. Nanus, Daniel A. Heller, Lorraine J. Gudas
Summary: In clear cell renal cell carcinoma (ccRCC), overexpression of NDUFA4L2 contributes to ccRCC proliferation and survival. NDUFA4L2 enhances glycolysis and regulates mitochondrial-lysosomal associations in ccRCC. Knocking out NDUFA4L2 decreases lysosomal size and abundance in ccRCC cells.
CANCER BIOLOGY & THERAPY
(2023)
Article
Chemistry, Physical
Daniel. E. Tylawsky, Hiroto Kiguchi, Jake Vaynshteyn, Jeffrey Gerwin, Janki Shah, Taseen Islam, Jacob. A. Boyer, Daniel. R. Boue, Matija Snuderl, Matthew. B. Greenblatt, Yosi Shamay, G. Praveen Raju, Daniel. A. Heller
Summary: This study demonstrates a novel nanotherapeutic approach using fucoidan-based nanocarriers to target the endothelial tumour vasculature and enhance blood-brain barrier crossing, achieving effective treatment of paediatric malignant brain tumours. The researchers also found that radiation treatment can increase the efficiency of this nanocarrier in penetrating the tumour microenvironment while minimizing toxicity to healthy brain tissue.
Meeting Abstract
Oncology
Magdalini Panagiotakopoulou, Emma Grabarnik, Edgar A. Jaimes, Marcel R. van den Brink, Daniel A. Heller
Meeting Abstract
Oncology
Arianna R. Izawa-Ishiguro, Ramya Sridharan, Christopher Wun, Tabassum J. Sami, Daniel A. Heller
Meeting Abstract
Obstetrics & Gynecology
Pietro Bortoletto, Mijin Kim, Declan Gwynne, Ewelina A. Randall, Daylon James, Daniel A. Heller
FERTILITY AND STERILITY
(2022)