Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond

Switching between nitrogen and glucose limitation: Unraveling transcriptional dynamics in Escherichia coli

(2017) Michael Löffler et al.   JOURNAL OF BIOTECHNOLOGY

Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions

(2017) Anja Lemoine et al.   JOURNAL OF BIOTECHNOLOGY

Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions

(2017) Annette Michalowski et al.   METABOLIC ENGINEERING

Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity

(2017) Dennis Binder et al.   METABOLIC ENGINEERING

Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce Long-Term Adaptation of E. coli Cultures in Large-Scale Bioreactors: Experimental Evidence and Mathematical Model

(2017) Alexander Nieß et al.   Frontiers in Microbiology

Transcriptional response of Escherichia coli to ammonia and glucose fluctuations

(2017) Joana Danica Simen et al.   Microbial Biotechnology

Metabolic profile of 1,5-diaminopentane producing Corynebacterium glutamicum under scale-down conditions: Blueprint for robustness to bioreactor inhomogeneities

(2016) Michael H. Limberg et al.   BIOTECHNOLOGY AND BIOENGINEERING

Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess

(2016) Yuanwei Gao et al.   Biotechnology Journal

Euler-Lagrange computational fluid dynamics for (bio)reactor scale down: An analysis of organism lifelines

(2016) Cees Haringa et al.   ENGINEERING IN LIFE SCIENCES

Engineering E. coli for large-scale production – Strategies considering ATP expenses and transcriptional responses

(2016) Michael Löffler et al.   METABOLIC ENGINEERING

Advancing microbial sciences by individual-based modelling

(2016) Ferdi L. Hellweger et al.   NATURE REVIEWS MICROBIOLOGY

Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess

(2016) Yuanwei Gao et al.   Biotechnology Journal

Response ofCorynebacterium glutamicumexposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor

(2015) Anja Lemoine et al.   BIOTECHNOLOGY AND BIOENGINEERING

Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law

(2015) Jonathan Baert et al.   Biotechnology Journal

Microbial individuality: how single-cell heterogeneity enables population level strategies

(2015) Bruno MC Martins et al.   CURRENT OPINION IN MICROBIOLOGY

Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law

(2015) Jonathan Baert et al.   Biotechnology Journal

CO2/HCO3 − perturbations of simulated large scale gradients in a scale-down device cause fast transcriptional responses in Corynebacterium glutamicum

(2014) Jens Buchholz et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Single-cell microfluidics: opportunity for bioprocess development

(2014) Alexander Grünberger et al.   CURRENT OPINION IN BIOTECHNOLOGY

Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process

(2014) Zhibiao Fu et al.   Microbial Cell Factories

Metabolic variability in bioprocessing: implications of microbial phenotypic heterogeneity

(2014) Frank Delvigne et al.   TRENDS IN BIOTECHNOLOGY

Assessment of robustness against dissolved oxygen/substrate oscillations for C. glutamicum DM1933 in two-compartment bioreactor

(2013) Friedrich Käß et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Flux response of glycolysis and storage metabolism during rapid feast/famine conditions inPenicillium chrysogenumusing dynamic13C labeling

(2013) Lodewijk de Jonge et al.   Biotechnology Journal

A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors

(2013) Alison Brognaux et al.   Microbial Cell Factories

Isolated Microbial Single Cells and Resulting Micropopulations Grow Faster in Controlled Environments

(2012) Christian Dusny et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level

(2012) Alexander Grünberger et al.   LAB ON A CHIP

A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: Impact of oscillations on Bacillus subtilis fed-batch cultivations

(2011) Stefan Junne et al.   Biotechnology Journal

Scale-down of penicillin production in Penicillium chrysogenum

(2011) Lodewijk P. de Jonge et al.   Biotechnology Journal

Real-time on-line flow cytometry for bioprocess monitoring

(2011) Tobias Broger et al.   JOURNAL OF BIOTECHNOLOGY

Separating intrinsic from extrinsic fluctuations in dynamic biological systems

(2011) A. Hilfinger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fast dynamic response of the fermentative metabolism ofEscherichia colito aerobic and anaerobic glucose pulses

(2009) Alvaro R. Lara et al.   BIOTECHNOLOGY AND BIOENGINEERING