Article
Chemistry, Analytical
Hongyu Zhou, Tiantian Liu, Jing Wang, Mingliang Liu, Yaoyao Lei, Xiaohua Tan, Ruhua Wang, Xijin Luo, Yeqing Chen
Summary: We propose a novel fluorescent approach for sensitive and label-free miRNA detection by integrating catalytic hairpin assembly and primer exchange reaction (PER) in a detection scaffold. This method shows high sensitivity and reliability, making it suitable for cancer diagnosis, prognosis, and treatment. The detection scaffold, constructed by hybridizing two hairpin structure probes (H1 and H2), can specifically recognize target miRNA and activate signal amplification. The PER process transcribes G-rich sequences to induce ThT-based label-free signal generation. The method exhibits a wide detection range (7 orders of magnitude) and high repeatability (coefficient of variation, 2.76%), making it a robust tool for miRNA quantification and early disease diagnosis.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Hongli Yan, Xiaohui Fan, Changmin Bai, Li Shan, Jing Chen, Ying Kang
Summary: MicroRNA (miRNA), a small non-coding RNA, is a potential biomarker for EC diagnosis. However, reliable detection of miRNA remains challenging, especially with methods that require multiple probes. In this study, a novel approach using a ternary hairpin probe (TH probe) was developed for miRNA-205 identification and quantification. The approach showed high efficiency signal amplification and specific target recognition, with a low limit of detection and a wide detection range.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuancong Xu, Nan Cheng, Yunbo Luo, Kunlun Huang, Qiaoying Chang, Guofang Pang, Wentao Xu
Summary: This study presents a self-fluorescence aptasensor for pesticide detection based on Exo III-assisted catalytic hairpin assembly. The sensor has high sensitivity and low background noise. By modifying the spatial configuration, the aptamer can specifically recognize the target and drive the cyclic amplification process, increasing the detection efficiency.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xuefeng Wang, Fengyi Wang, Jianrong Wang, Yunqing Liu, Chaomin Gao, Shenguang Ge, Jinghua Yu
Summary: Herein, a highly sensitive and specific photoelectrochemical biosensor is constructed using CRISPR/Cas12a and catalytic hairpin assembly (CHA) non-enzyme amplification technology. The sensor, using Bi/g-C3N4 nanohybrids as a composite electrode, demonstrates accurate detection of miRNA-122 with the help of multiple amplification mechanisms. The universal CRISPR/Cas12a cutting characteristics further expand its analysis potential and simplify the operation difficulty, showing great potential in biomedical detection and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Xia Li, Fang Yang, Chunfang Gan, Ruo Yuan, Yun Xiang
Summary: The researchers have developed a novel DNA hairpin assembly method by introducing palindrome sequences, which can image miRNA-155 in living tumor cells with high sensitivity, providing a new approach for early disease diagnosis and biological studies.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yan Wu, Cuicui Fu, Wenbing Shi, Jinyang Chen
Summary: Abnormal expression of miRNAs is closely associated with health disorders, making them potential biomarkers for early clinical diagnosis. The enzyme-free CHA signal amplification method shows significant potential in improving the sensitivity of miRNA detection strategies, with various sensing strategies based on this approach being developed.
Article
Chemistry, Analytical
Furui Jin, Danke Xu
Summary: The mi-CHA method detects miRNAs by changing the conformation of hairpin structured DNA probes on the chip surface, showing high sensitivity and specificity for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Yaxing Liu, Wei Guo, Yunzhe Zhang, Xin Lu, Qian Yang, Wei Zhang
Summary: An accurate and ultrasensitive ratiometric electrochemical aptasensor based on catalytic hairpin assembly (CHA) was developed for the detection of Ochratoxin A (OTA) pollution in agricultural products. This aptasensor achieved trace-level detection of OTA with a LOD of 81 fg/mL and a linear range of lower concentration (100 fg/mL-50 ng/mL). The strategy was successfully applied to OTA detection in cereals and provided comparable results to HPLC-MS. This aptasensor offers a viable platform for accurate, ultrasensitive, and one-step detection of OTA in food.
Article
Spectroscopy
Ying Li, Xinyue Ma, Kai Liu, Zheng Liu, Ruiqi Zou, Junyang Wang, Chuanyu Yang, Hongru Zheng, Chunyan Sun
Summary: Heavy metal pollution poses a threat to food safety and human health, making accurate quantification of heavy metal ions essential. DNA nanostructures-based biosensors offer attractive tools for sensitive or rapid analysis of heavy metal ions. However, their complex design, severe reaction conditions, and unreliable performance hinder their advancement and application.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Ya-Xin Wang, Dong-Xia Wang, Jing Wang, Bo Liu, An-Na Tang, De-Ming Kong
Summary: The study developed a highly integrated DNA nanoamplifier by anchoring hairpin probes on the surface of DNA nanolantern, enabling high sensitivity detection of miRNAs. The nanoamplifier showed enhanced signal amplification capability, accelerated reaction kinetics, and enabled miRNA imaging and analysis in living cells and mice.
Article
Biophysics
Pinru Chen, Luying Wang, Peipei Qin, Bin-Cheng Yin, Bang-Ce Ye
Summary: In this study, a one-step detection method for microRNAs based on the RNA-based catalytic hairpin assembly (CHA) circuit coupled with CRISPR-Cas12a was proposed. The target miRNA can specifically trigger RNA-based CHA and induce a configurational change of the blocked crRNAs into precursor crRNAs (pre-crRNAs), which can be processed into mature crRNAs to function by leveraging the inherent RNase activities of Cas12a. The developed circuit achieves a femtomolar detection limit and shows accurate detection of miRNA levels in different cell lines.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: In this study, an enzyme-free signal amplification method for miRNA detection was developed using target-triggered catalytic hairpin assembly (CHA). The method showed high sensitivity and fast detection speed, with good recoveries in serum samples.
Article
Chemistry, Analytical
Liuting Mo, Mingxiu Mo, Danlian Liang, Chan Yang, Weiying Lin
Summary: Improving the accuracy, sensitivity, and speed of intracellular miRNA imaging is crucial for early cancer diagnosis. This study proposes a strategy for imaging two distinct miRNAs using DNA tetrahedron-based catalytic hairpin assembly (DCHA). Two nanoprobes, DTH-13 and DTH-24, were prepared and their structures were functionalized DNA tetrahedrons with CHA hairpins responsive to miR-21 and miR-155. These DNA nanoparticles were used as carriers to easily enter living cells. The presence of miR-21 or miR-155 triggered CHA between DTH-13 and DTH-24, resulting in independent fluorescence signals. The sensitivity and kinetics of this system were significantly enhanced due to the DCHA strategy. The performance of this method was thoroughly investigated in various samples, and the results confirmed the potential of DTH nanoprobes as a diagnostic tool for early-stage cancer.
Article
Chemistry, Analytical
Xiaojia Zhao, Zicheng Zhu, Rong Zou, Lingyun Wang, Hang Gong, Changqun Cai
Summary: A 3D DNA walker was developed for the reliable and quantitative detection of H5N1 DNA, demonstrating excellent analytical sensitivity and specificity with a detection limit of 60 pM. The method showed potential for clinical applications by selectively detecting H5N1 DNA in clinical blood serum samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Su Jiang, Qian Liu, Juan Hu, Dong Yuan, Yan Zhang, Chun-yang Zhang
Summary: In this study, a novel method was developed for sensitive detection of miRNA in lung tissues by target-triggered assembly of G-quadruplex DNAzyme nanowires. This method exhibited high sensitivity, a large dynamic range, and the ability to distinguish single-base mutations, holding promising applications in biomedical analysis and cancer prognosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
