Article
Microbiology
Melanie D. Balhuizen, Albert van Dijk, Jeroen W. A. Jansen, Chris H. A. van de Lest, Edwin J. A. Veldhuizen, Henk P. Haagsman
Summary: Host defense peptides (HDPs) are part of the innate immune system and possess antimicrobial activity. This study found that certain HDPs induce the release of outer membrane vesicles (OMVs) by bacteria. OMVs act as a means for bacteria to dispose of HDP-affected membrane and act as decoys to protect the bacterium from HDPs.
Article
Medicine, Research & Experimental
Megha Srinivas, Preeti Sharma, Siddharth Jhunjhunwala
Summary: Research findings suggest that immune cells can phagocytose particles under constant fluidic flow. Different cell types show similar or better uptake rates under flow conditions. Even in the presence of crowded red blood cells, neutrophils and monocytes are able to uptake particles while flowing.
MOLECULAR PHARMACEUTICS
(2021)
Article
Immunology
Wiriya Maisat, Xiaohui Han, Sophia Koutsogiannaki, Sulpicio G. Soriano, Koichi Yuki
Summary: This study tested the effects of dexmedetomidine on human monocytes and found that it improved phagocytosis and killing of Gram-positive bacteria, but had a weaker effect on Gram-negative bacteria. Dexmedetomidine may inhibit the TLR4-signaling pathway and mitigate the alternative phagocytosis pathway induced by TLR4 activation through LPS-mediated Gram-negative bacteria. This research provides a novel insight into the potential risks of dexmedetomidine use during Gram-negative infections.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Hongyan Yang, Lan Wang, Libo Yuan, Heng Du, Boyuan Pan, Kui Lu
Summary: In this study, a series of hybrid peptides were designed by connecting an antimicrobial peptide Ce(1-8) with a lipopolysaccharide-targeting peptide Lf(28-34) via different linkers. The results showed that the linkers played a crucial role in the antimicrobial activity of the hybrid peptides against Gram-negative bacteria. Among these hybrid peptides, peptide CL5 with a dipeptide rigid linker exhibited excellent activity and selectivity against Gram-negative bacteria. It was found that the interaction of the peptides with LPS was crucial for their antimicrobial activity. Mechanistic studies revealed that peptide CL5 disrupted the integrity of bacterial membranes, leading to the death of Gram-negative bacterial cells. This study highlights the importance of linker selection in the design of hybrid peptides and provides a foundation for the development of antimicrobial peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Frederic V. Schwabe, Lotta Happonen, Sofie Ekestubbe, Ariane Neumann
Summary: This study investigated the effects of LL-37 and LTF on blood-derived CD14(+) monocytes and found that these HDPs can induce the release of TNF alpha, nucleosomes, and monocyte ETs. The formation of ETs by LL-37 is dependent on SOCE, MAPK, and ERK1/2, while the release of ETs by LTF is not affected by any inhibitors used in this study.
Article
Biochemistry & Molecular Biology
Kunhong Zhong, Yuelong Wang, Zeng Wang, Zongliang Zhang, Shasha Zhao, Hexian Li, Jianhan Huang, Wenhao Guo, Xi Zheng, Gang Guo, Liangxue Zhou, Hui Yang, Aiping Tong
Summary: The study identified a human antimicrobial peptide called AP-64, which displayed significant antibacterial activity against Gram-negative bacteria, effectively combating Escherichia coli O157:H7 infections and exhibiting cytotoxic effects against human lymphoma cells. Additionally, a mouse homologous gene encoded peptide Gm94 showed similar antibacterial properties to AP-64.
Review
Infectious Diseases
Jawid Nazir Ahmad, Peter Sebo
Summary: RTX toxins play a crucial role in bacterial infections by inhibiting phagocytic cells, dampening inflammatory responses, compromising epithelial barrier, and reducing the phagocytic ability of macrophages, thus promoting bacterial survival and proliferation in the host.
CURRENT OPINION IN INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Anne E. Geller, Rejeena Shrestha, Matthew R. Woeste, Haixun Guo, Xiaoling Hu, Chuanlin Ding, Kalina Andreeva, Julia H. Chariker, Mingqian Zhou, David Tieri, Corey T. Watson, Robert A. Mitchell, Huang-ge Zhang, Yan Li, Robert C. G. Martin, Eric C. Rouchka, Jun Yan
Summary: The application of immunotherapy in pancreatic ductal adenocarcinoma has been limited, but researchers have found that fungal beta-glucan can induce trained immunity, resulting in reduced tumor burden and improved survival in mouse models. This study highlights the importance of innate immune cells in anti-tumor immunosurveillance and suggests a potential application of trained immunity in cancer therapy.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Sarah To, Thandiwe Chavula, Mesias Pedroza, Jennifer Smith, Sandeep K. Agarwal
Summary: CDH11 plays an important role in pulmonary fibrosis by regulating macrophage development, M2 polarization, and phagocytic function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Benjamin S. Prior, Miles D. Lange, Scott A. Salger, Benjamin J. Reading, Eric Peatman, Benjamin H. Beck
Summary: Fish-derived antimicrobial peptides, particularly piscidins, have demonstrated potent antimicrobial activity against Gram-negative and Gram-positive bacterial species, with class II piscidins showing greater inhibition against Escherichia coli and Flavobacterium columnare biofilms compared to class I piscidins. However, both classes of piscidins were less effective against inhibiting biofilm growth of Aeromonas hydrophila and A. veronii.
JOURNAL OF FISH DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Manoj Puthia, Jan K. Marzinek, Ganna Petruk, Gizem Erturk Bergdahl, Peter J. Bond, Jitka Petrlova
Summary: This study demonstrates that APOE has anti-bacterial activity against several Gram-negative bacterial strains and exhibits significant binding affinity for lipopolysaccharide and heparin. Molecular dynamics simulations reveal the binding region in helix 4 of APOE as a primary site for interaction with these molecules through electrostatic interactions.
Article
Biochemistry & Molecular Biology
Ariana Diaz, Magdalena Diab, Dulce Mata-Espinosa, Estela Bini, Luciano D'Attilio, Oscar Bottasso, Rogelio Hernandez-Pando, Maria Luisa Bay, Bettina Bongiovanni
Summary: This study investigates the reciprocal influence of cortisol and dehydroepiandrosterone (DHEA) on host defense peptides (HDPs) biosynthesis and the effect of LL-37 on adrenal steroidogenesis. The results show that cortisol reduces the production of HDPs in mycobacteria-stimulated macrophages, while DHEA increases reactive oxygen species (ROS) levels and reduces intracellular mycobacterial growth. LL-37 also affects the production of cortisol and DHEA in adrenal cells.
Review
Chemistry, Medicinal
Tamara Fedorovna Solov'eva, Svetlana Ivanovna Bakholdina, Gennadii Alexandrovich Naberezhnykh
Summary: This article summarizes the potential of LPS-binding proteins from marine invertebrates as drugs for sepsis treatment. The structure, properties, and activity of these proteins, as well as the potential problems in clinical trials are discussed in detail.
Review
Gastroenterology & Hepatology
Yingping Xiao, Huicong Zou, Jingjing Li, Tongxing Song, Wentao Lv, Wen Wang, Zhenyu Wang, Shiyu Tao
Summary: Quorum sensing is a vital molecular signaling mechanism in bacteria that regulates microbial community behavior and host cell function. Blocking quorum sensing signaling molecules may present opportunities for combating diseases.
Article
Multidisciplinary Sciences
Kumar Sachin Singh, Rishabh Sharma, Poli Adi Narayana Reddy, Prashanthi Vonteddu, Madeline Good, Anjana Sundarrajan, Hyeree Choi, Kar Muthumani, Andrew Kossenkov, Aaron R. Goldman, Hsin-Yao Tang, Maxim Totrov, Joel Cassel, Maureen E. Murphy, Rajasekharan Somasundaram, Meenhard Herlyn, Joseph M. Salvino, Farokh Dotiwala
Summary: Isoprenoids are essential for maintaining cellular functions and IspH is a crucial enzyme in their synthesis pathway. Through structure-guided design, a class of IspH inhibitors with nanomolar potency were identified, showing efficacy against multidrug-resistant bacteria while being relatively non-toxic to mammalian cells. These prodrugs also induce expansion and activation of human V gamma 9V delta 2 T cells, suggesting a potential strategy to limit antibiotic-resistant bacterial populations.
Article
Multidisciplinary Sciences
Mariena J. A. van der Plas, Ravi K. V. Bhongir, Sven Kjellstrom, Helena Siller, Gopinath Kasetty, Matthias Morgelin, Artur Schmidtchen
NATURE COMMUNICATIONS
(2016)
Article
Multidisciplinary Sciences
Jitka Petrlova, Finja C. Hansen, Mariena J. A. van der Plas, Roland G. Huber, Matthias Morgelin, Martin Malmsten, Peter J. Bond, Artur Schmidtchen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Rathi Saravanan, Sunil S. Adav, Yeu Khai Choong, Mariena J. A. van der Plas, Jitka Petrlova, Sven Kjellstrom, Siu Kwan Sze, Artur Schmidtchen
SCIENTIFIC REPORTS
(2017)
Article
Nanoscience & Nanotechnology
Sara Malekkhaiat Haffner, Lina Nystrom, Kathryn L. Browning, Hanne Morck Nielsen, Adam A. Stromstedt, Mariena J. A. van der Plas, Artur Schmidtchen, Martin Malmsten
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Biochemistry & Molecular Biology
Jitka Petrlova, Ganna Petruk, Roland G. Huber, Eilish W. McBurnie, Mariena J. A. van der Plas, Peter J. Bond, Manoj Puthia, Artur Schmidtchen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Chemistry, Physical
Sara Malekkhaiat Haffner, Lina Nystrom, Adam A. Stromstedt, Li Li, Mariena J. A. van der Plas, Martin Malmsten
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Immunology
Erik Hartman, Karl Wallblom, Mariena J. A. van der Plas, Jitka Petrlova, Jun Cai, Karim Saleh, Sven Kjellstrom, Artur Schmidtchen
Summary: The study investigates the peptidomes of wound fluids and reveals highly individualized profiles in patients, with differences based on wound type. Peptides from infected wounds may contribute to an antimicrobial environment. Validation of findings through literature compilation and cross checking against data confirms the presence of immunologically significant peptides in infected wounds. The use of sorting algorithms and open source software demonstrates the power to analyze and visualize peptidomic data.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Humaira Ilyas, Mariena J. A. van der Plas, Monica Agnoletti, Sourav Kumar, Atin Kumar Mandal, Hanudatta S. Atreya, Anirban Bhunia, Martin Malmsten
Summary: The study investigated how PEGylation affects the interaction of host defense peptides with bacterial lipopolysaccharide and the suppression of LPS-induced cell activation. The results showed that PEGylation did not compromise the anti-inflammatory effects of the peptides and even improved the suppression of LPS-induced cell activation.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Biology
Mariena J. A. van der Plas, Jun Cai, Jitka Petrlova, Karim Saleh, Sven Kjellstrom, Artur Schmidtchen
Summary: The study characterized the peptidome of human wound fluids using mass-spectrometry analyses for the first time, revealing a higher degree of peptides in sterile post-surgical wound fluids compared to human plasma. The unique peptide patterns identified in uninfected healing wounds and Staphylococcus aureus-infected wounds suggest the potential of using these peptides as biomarkers for wound healing and infection.
Article
Immunology
Finja C. Hansen, Aftab Nadeem, Kathryn L. Browning, Mario Campana, Artur Schmidtchen, Mariena J. A. van der Plas
Summary: The study found that the internalization pathways of C-terminal host defense peptides (TCPs) in monocytes and macrophages are complex, involving both clathrin-dependent and clathrin-independent pathways, and mainly dynamin-dependent pathway in monocytes. Furthermore, the internalization of TCPs is also influenced by factors such as peptide length and composition, the presence of lipopolysaccharides (LPS), and bacteria.-recognized TCPs.
JOURNAL OF INNATE IMMUNITY
(2021)
Article
Pharmacology & Pharmacy
Alma Martin, Jun Cai, Anna-Lena Schaedel, Mariena van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: This study aims to develop fibers with enhanced mechanical strength and water stability, as well as sustained release of tetracycline hydrochloride. By using zein as core material and polycaprolactone as shell material, the researchers achieved improved cell attachment and wound closure, while maintaining continuous drug release.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Alma Martin, Jenny Natalie Nyman, Rikke Reinholdt, Jun Cai, Anna-Lena Schaedel, Mariena J. A. van der Plas, Martin Malmsten, Thomas Rades, Andrea Heinz
Summary: Nanofiber-reinforced hydrogels have attracted attention in biomedical engineering due to their mechanical strength, water absorption properties, and potential for promoting cell migration. This study demonstrates a one-step solution for producing nanofiber-reinforced hydrogels using zein and gelatin proteins. The resulting nanofiber-hydrogel composite showed significant improvements in swelling and physical integrity compared to hydrogels without nanofibers. Additionally, the incorporation of antimicrobial agents into the fibers proved effective against Staphylococcus aureus and Escherichia coli.
Editorial Material
Pharmacology & Pharmacy
Artur Schmidtchen, Haris Mirza, Mariena J. A. van der Plas, Aftab Nadeem, Manoj Puthia
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Dermatology
S. Eriksson, M. J. A. van der Plas, M. Morgelin, A. Sonesson
BRITISH JOURNAL OF DERMATOLOGY
(2017)