Establishment of expanded and streamlined pipeline of PITCh knock-in – a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO

Cas9, Cpf1 and C2c1/2/3―What's next?

(2017) Shota Nakade et al.   Bioengineered

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

(2016) Tomomi Aida et al.   BMC GENOMICS

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

Analyzing CRISPR genome-editing experiments with CRISPResso

(2016) Luca Pinello et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

(2015) Tetsushi Sakuma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

(2015) Tetsushi Sakuma et al.   Nature Protocols

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

(2015) Yu Hisano et al.   Scientific Reports

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Nuclease-mediated genome editing: At the front-line of functional genomics technology

(2014) Tetsushi Sakuma et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

(2014) Shota Nakade et al.   Nature Communications

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY