期刊
FRONTIERS IN PHYSIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2017.00839
关键词
LETM1; mitochondrial cation/proton exchange; mitochondrial volume homeostasis; potassium; sodium; calcium
类别
资金
- FWF Austrian Science Fund [P24271]
- DOC fellowship of the Austrian Academy of Sciences
- Italian Ministry of Education, University and Research (MIUR) (PRIN) [20107Z8XBW_004]
- Italian Ministry of Education, University and Research (MIUR) (PRONAT project)
- CNR (Project of Special Interest on Aging and InterOmics project)
- University of Padova
- MIUR
- Austrian Science Fund (FWF) [P24271] Funding Source: Austrian Science Fund (FWF)
Ca2+ transport across the inner membrane of mitochondria (IMM) is of major importance for their functions in bioenergetics, cell death and signaling. It is therefore tightly regulated. It has been recently proposed that LETM1-an IMM protein with a crucial role in mitochondrial K+/H+ exchange and volume homeostasis-also acts as a Ca2+/H+ exchanger. Here we show for the first time that lowering LETM1 gene expression by shRNA hampers mitochondrial K+/H+ and Na+/H+ exchange. Decreased exchange activity resulted in matrix K+ accumulation in these mitochondria. Furthermore, LETM1 depletion selectively decreased Na+/Ca2+ exchange mediated by NCLX, as observed in the presence of ruthenium red, a blocker of the Mitochondrial Ca2+ Uniporter (MCU). These data confirm a key role of LETM1 in monovalent cation homeostasis, and suggest that the effects of its modulation on mitochondrial transmembrane Ca2+ fluxes may reflect those on Na+/H+ exchange activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据