Article
Multidisciplinary Sciences
Sarah J. Brown, Ibrahim Boussaad, Javier Jarazo, Julia C. Fitzgerald, Paul Antony, Marcus Keatinge, Janna Blechman, Jens C. Schwamborn, Rejko Krueger, Marysia Placzek, Oliver Bandmann
Summary: Recent evidence suggests that neurogenesis continues throughout life but the impact on neurodegenerative disorders like Parkinson's disease is still unclear. Studies in zebrafish and human organoids show that PINK1 deficiency impairs adult dopaminergic neurogenesis, potentially affecting future therapeutic approaches for PD patients with biallelic PINK1 mutations.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Sara Zocher, Rupert W. Overall, Gabriel Berdugo-Vega, Nicole Rund, Anne Karasinsky, Vijay S. Adusumilli, Christina Steinhauer, Sina Scheibenstock, Kristian Handler, Joachim L. Schultze, Federico Calegari, Gerd Kempermann
Summary: Newborn DNA methylation during adult neurogenesis is crucial for the maturation and integration of functional neurons in the hippocampus, facilitating transcriptional up-regulation of neuronal genes and affecting activation patterns in the hippocampal circuitry.
Article
Neurosciences
Li He, Huihui Zheng, Jilong Qiu, Hong Chen, Huan Li, Yuejiao Ma, Yingying Wang, Qianjin Wang, Yuzhu Hao, Yueheng Liu, Qian Yang, Xin Wang, Manyun Li, Huixue Xu, Pu Peng, Zejun Li, Yanan Zhou, Qiuxia Wu, Shubao Chen, Xiaojie Zhang, Tieqiao Liu
Summary: This study identified significant effects of methamphetamine on enteric dopaminergic neurons, with variations depending on the location. These findings may be relevant to the potential effects of methamphetamine on intestinal functions, such as motility.
NEUROTOXICITY RESEARCH
(2023)
Review
Cell Biology
Werend Boesmans, Amelia Nash, Kinga R. Tasnady, Wendy Yang, Lincon A. Stamp, Marlene M. Hao
Summary: Enteric glia are support cells of the enteric nervous system that play a vital role in regulating gut functions and maintaining homeostasis. Their plasticity and heterogeneity make them neural precursors with the potential for neurogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rhian Stavely, Leah C. Ott, Niloufar Rashidi, Samy Sakkal, Kulmira Nurgali
Summary: Oxidative stress plays a critical role in gastrointestinal disorders, causing injury and dysfunction in the enteric nervous system, and offers potential opportunities for targeted therapeutics.
Article
Multidisciplinary Sciences
Thomas Parmentier, Fiona M. K. James, Elizabeth Hewitson, Craig Bailey, Nicholas Werry, Steven D. Sheridan, Roy H. Perlis, Melissa L. Perreault, Luis Gaitero, Jasmin Lalonde, Jonathan LaMarre
Summary: Activity-induced neurogenesis has been widely studied in rodents but less in humans. In this study, neuronal activity and associated neurogenesis were investigated in cerebral spheroids derived from human induced pluripotent stem cells (iPSCs). The results show that 4AP increases neuronal activity, promotes the generation of new neurons, and reduces the number of new glial cells. Furthermore, the expression of miR-135a, a key regulator of exercise-induced neurogenesis, was significantly decreased, suggesting its involvement in the process. These findings highlight the potential of iPSC-derived cerebral spheroids as a model for studying activity-induced neurogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Feng Zhang, Kijun Yoon, Nam-Shik Kim, Guo-li Ming, Hongjun Song
Summary: Quiescence is important for adult neural stem cells (NSCs) in the mammalian brain, but how NSCs in the hippocampus acquire and maintain quiescence is not well understood. This study showed that deleting Nkcc1, a chloride importer, in NSCs impaired their acquisition of quiescence during early postnatal stages and their maintenance of quiescence in adulthood. Additionally, deleting Nkcc1 in interneurons led to activation of quiescent NSCs and an expanded NSC pool. Inhibition of NKCC1 also promoted NSC proliferation. These findings highlight the roles of NKCC1 in regulating NSC quiescence in the mammalian hippocampus.
Article
Biochemistry & Molecular Biology
Adrien de Guilhem de Lataillade, Martial Caillaud, Thibauld Oullier, Philippe Naveilhan, Carolina Pellegrini, Eduardo Tolosa, Michel Neunlist, Malvyne Rolli-Derkinderen, Ellen Gelpi, Pascal Derkinderen
Summary: The LRRK2 gene is associated with both Parkinson's disease (PD) and Crohn's disease, suggesting its role in gastrointestinal inflammation. However, there is limited research on LRRK2 in the enteric nervous system (ENS). This study characterizes LRRK2 expression in the ENS, revealing its presence in enteric glial cells and identifying the cAMP pathway as a key regulator of LRRK2 expression in these cells. The findings provide a basis for further understanding the functions of LRRK2 in the ENS and investigating pathological changes in enteric synucleinopathies.
JOURNAL OF NEUROCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Richard A. Guyer, Jessica L. Mueller, Allan M. Goldstein
Summary: With the advancement of technology, single-cell sequencing has become a common tool to analyze cellular diversity and reveal cellular diversity within organs. The application of these methods in the study of the enteric nervous system has provided abundant data and biological insights. As technology continues to evolve, these methods may become commonplace in enteric nervous system research and diagnostic testing.
Review
Nutrition & Dietetics
Patricia Pereira Almeida, Ana Lucia Tavares-Gomes, Milena Barcza Stockler-Pinto
Summary: The enteric nervous system (ENS) regulates functions and immune processes in the gastrointestinal tract. Diseases can disrupt ENS functionality by increasing reactive oxygen species accumulation, impacting enteric neurons and glial cells. Nutritional strategies aimed at reducing reactive oxygen species production can modulate the ENS, and studies have shown that nutrients with antioxidant roles have potential in this regard.
Article
Anatomy & Morphology
Mireia Cara-Esteban, Maria Pilar Marin, Emma Martinez-Alonso, Sergio Martinez-Bellver, Vicent Teruel-Marti, Jose A. Martinez-Menarguez, Monica Tomas
Summary: Gastrointestinal disorders are early consequences of Parkinson's disease and significantly affect the enteric nervous system. The Golgi complexes of enteric dopaminergic neurons appear fragmented in a rat model of Parkinson's disease, suggesting their potential as early indicators of the disease.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Cell Biology
Miguel Gonzalez Acera, Marvin Bubeck, Fabrizio Mascia, Leonard Diemand, Gregor Sturm, Anja A. Kuehl, Raja Atreya, Dieter Chichung Lie, Markus F. Neurath, Michael Schumann, Christoph S. N. Klose, Zlatko Trajanoski, Christoph Becker, Jay Patankar
Summary: Inflammatory bowel diseases cause an increase in the transcript levels of enteric neuronal and glial genes, with elevated neuronal innervation of inflamed regions of the gut mucosa. However, this induction is transient and levels return to normal upon remission induction, highlighting the dynamic and robust nature of the enteric nervous system in colitis.
Article
Neurosciences
Aleksandar Rakovic, Dorothea Voss, Franca Vulinovic, Britta Meier, Ann-Katrin Hellberg, Carla Nau, Christine Klein, Enrico Leipold
Summary: Induced pluripotent stem cells can be used to create patient-specific in vitro models for studying Parkinson's disease and drug screening. However, current differentiation methods result in heterogeneous cell populations with low fractions of relevant dopaminergic neurons. This study utilized CRISPR/Cas9 technology to generate a reporter iPSC line and compared different differentiation protocols. The floor plate protocol was found to be the most efficient in generating electrophysiologically mature dopaminergic neurons.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Gastroenterology & Hepatology
Hiroyuki Nakamori, Kenta Noda, Retsu Mitsui, Hikaru Hashitani
Summary: The roles of enteric dopaminergic neurons in the generation of colonic peristalsis were investigated by studying the effects of various neurotransmitter blockers on peristaltic waves in rat colonic segments. Dopaminergic neurons appeared to facilitate nitrergic neurons via D-1-like receptors to stabilize asynchronous contractile activity resulting in the generation of colonic peristalsis.
NEUROGASTROENTEROLOGY AND MOTILITY
(2021)
Article
Neurosciences
Saak Ovsepian, Valerie B. O'Leary
Summary: Autoantibodies to neuronal antigens are potential biomarkers for neurodegenerative diseases. Recent evidence suggests a dissociation between the neurodegenerative process in the central nervous system and the dynamics of neuronal proteins in the peripheral circulation. The release of neuronal proteins from the enteric nervous system may explain the presence of immunoglobulins reactive to neuronal antigens in the blood of healthy subjects.
Review
Cell Biology
Karla Mendez-Maldonado, Guillermo A. Vega-Lopez, Manuel J. Aybar, Ivan Velasco
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Multidisciplinary Sciences
Maria Jose Castellanos-Montiel, Ivan Velasco, Itzel Escobedo-Avila
Summary: The neuromuscular junction (NMJ) is a specialized structure that translates the action potential of the presynaptic motor neuron (MN) into the contraction of the postsynaptic myofiber. Different experimental models have been developed to study NMJ function and diseases, with current approaches including 3D structures and biomaterial-incorporated tissues.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Neurosciences
Jose Romo-Yanez, Griselda Rodriguez-Martinez, Jorge Aragon, Lourdes Siqueiros-Marquez, Alma Herrera-Salazar, Ivan Velasco, Cecilia Montanez
NEUROSCIENCE LETTERS
(2020)
Article
Cell & Tissue Engineering
Andrea Tenorio-Mina, Daniel Cortes, Joel Esquivel-Estudillo, Adolfo Lopez-Ornelas, Alejandro Cabrera-Wrooman, Rolando Lara-Rodarte, Itzel Escobedo-Avila, Fernanda Vargas-Romero, Diana Toledo-Hernandez, Enrique Estudillo, Juan Jose Acevedo-Fernandez, Jesus Santa-Olalla Tapia, Ivan Velasco
Summary: Studies have shown that human keratinocytes can express neural cell characteristic genes after culturing in neural medium, but in vitro differentiation did not yield neuronal or astrocytic markers. However, when these cells were grafted into the developing CNS of rats, both control and neural-induced cells expressed neuronal proteins, suggesting the in vivo environment provides appropriate signals for evaluating the neuronal differentiation potential of keratinocytes or other non-neural cell populations.
CELL TRANSPLANTATION
(2021)
Review
Cell & Tissue Engineering
Daniel Cortes, Martin F. Pera
Summary: Traumatic brain injury (TBI) is a major cause of death among young people and is increasingly common in the elderly population. The outcomes for TBI survivors vary widely, leading to a new emphasis on developing personalized medicine approaches in TBI treatment and a better understanding of how genetics impact long-term outcomes. Studies in animal models and human candidate gene approaches have identified factors influencing recovery, pointing towards the development of new therapeutic approaches.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Alain Aguirre-Vazquez, Luis A. Salazar-Olivo, Xochitl Flores-Ponce, Ana L. Arriaga-Guerrero, Dariela Garza-Rodriguez, Maria E. Camacho-Moll, Ivan Velasco, Fabiola Castorena-Torres, Nidheesh Dadheech, Mario Bermudez de Leon
Summary: By utilizing epigenetic molecules and small molecules, the expression of pluripotency genes can be induced and methylation in the OCT4 promoter can be reduced in human somatic cells, enhancing reprogramming efficiency. Further studies are needed to elucidate the mechanisms by which these compounds exert their effects on reprogramming.
Article
Multidisciplinary Sciences
Cesar Melendez-Ramirez, Raquel Cuevas-Diaz Duran, Tonatiuh Barrios-Garcia, Mayela Giacoman-Lozano, Adolfo Lopez-Ornelas, Jessica Herrera-Gamboa, Enrique Estudillo, Ernesto Soto-Reyes, Ivan Velasco, Victor Trevino
Summary: Chromatin architecture plays a key role in influencing transcription during dopaminergic neuron differentiation, with a high abundance of long non-coding RNAs in the most downregulated transcripts. Open chromatin regions decrease during differentiation and are associated with specific functional pathways and gene-sets. The study also identifies potential transcription factors and structural nuclear proteins involved in regulating dopaminergic differentiation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Rolando Lara-Rodarte, Daniel Cortes, Karla Soriano, Francia Carmona, Luisa Rocha, Enrique Estudillo, Adolfo Lopez-Ornelas, Ivan Velasco
Summary: This study engineered mouse ESCs to produce human GDNF, showing that overexpression of GDNF contributes to enhanced midbrain differentiation and increased survival of DaNs after grafting into the striatum.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Enrique Estudillo, Adriana Jimenez, Pablo Edson Bustamante-Nieves, Carmen Palacios-Reyes, Ivan Velasco, Adolfo Lopez-Ornelas
Summary: The process of cryopreservation involves freezing cells or tissues in liquid nitrogen, affecting cell viability, proteomic profile changes, and crucial cellular functions. While widely used since the 1980s, there is still a lack of knowledge about molecular changes associated with cryopreservation, impacting metabolism, cytoarchitecture, calcium homeostasis, epigenetic state, and compromising fertilization in assisted reproductive technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Adolfo Lopez-Ornelas, Adriana Jimenez, Gilberto Perez-Sanchez, Citlali Ekaterina Rodriguez-Perez, Alejandro Corzo-Cruz, Ivan Velasco, Enrique Estudillo
Summary: Alzheimer's disease is a common neurodegenerative disorder with increasing prevalence. Understanding its pathophysiology is crucial for developing effective treatment. Despite recent efforts in drug development, the neuronal damage caused by Amyloid beta peptide and Tau protein abnormalities remains a challenge. Peripheral inflammation is closely associated with the onset and progression of AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Karla Sanchez-Huerta, Rosaura Debbie Saldana-Salinas, Pablo Edson Bustamante-Nieves, Adriana Jimenez, Alejandro Corzo-Cruz, Marina Martinez-Vargas, Rosalinda Guevara-Guzman, Ivan Velasco, Enrique Estudillo
Summary: This study found that sucrose intake during late adolescence does not induce anxiety, but does increase cell proliferation in the ventral dentate gyrus. Although there were no differences in the number of immature granular neurons in the hippocampus of rats exposed to sucrose, more immature neurons with impaired dendritic orientation were observed in both groups exposed to sucrose.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Adolfo Lopez-Ornelas, Itzel Escobedo-Avila, Gabriel Ramirez-Garcia, Rolando Lara-Rodarte, Cesar Melendez-Ramirez, Beetsi Urrieta-Chavez, Tonatiuh Barrios-Garcia, Veronica A. Caceres-Chavez, Xochitl Flores-Ponce, Francia Carmona, Carlos Alberto Reynoso, Carlos Aguilar, Nora E. Kerik, Luisa Rocha, Leticia Verdugo-Diaz, Victor Trevino, Jose Bargas, Veronica Ramos-Mejia, Juan Fernandez-Ruiz, Aurelio Campos-Romo, Ivan Velasco
Summary: Research on transplanting DANs obtained from human embryonic stem cells into parkinsonian monkeys showed that these neurons can survive in the brain, improve some motor behaviors in animals, and exhibit a negative correlation with FA.
Review
Medicine, Research & Experimental
Santiago Cerrizuela, Guillermo A. Vega-Lopez, Karla Mendez-Maldonado, Ivan Velasco, Manuel J. Aybar
Summary: Animal models are valuable in studying normal and pathological development at the molecular, cellular, and morphogenetic levels. Cell-based models offer an alternative approach to understanding human embryonic development and diseases. Neural crest (NC) cells are transient, multipotent embryonic cells that play a crucial role in generating various cell types during vertebrate development, and abnormalities in NC development can lead to neurocristopathies with diverse functional and morphological alterations.
WIRES MECHANISMS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Jose Romo-Yanez, Griselda Rodriguez-Martinez, Jorge Aragon, Lourdes Siqueiros-Marquez, Alma Herrera-Salazar, Ivan Velasco, Cecilia Montanez
Summary: This study utilized neural stem/progenitor cells to investigate the differentiation of neurons and astrocytes, as well as evaluate the expression of dystrophins. The findings are expected to enhance our understanding of the expression and function of dystrophins during neuronal and astrocytic differentiation.
