Article
Developmental Biology
Daniel B. Dranow, Pierre Le Pabic, Thomas F. Schilling
Summary: Non-canonical/beta-catenin-independent Wnt signaling is crucial for tissue/cell polarity in epithelia, but its role in mesenchymal tissues like the skeleton is not well studied. Mutations in non-canonical Wnt signaling pathway genes cause skeletal diseases in humans, disrupting bone growth. Ror2 is a non-canonical Wnt receptor/co-receptor. Zebrafish with ror2-/- mutation show craniofacial skeletal defects and chondrocyte polarity disruptions. The proline-rich domain of Ror2 is required for its function in rescuing the defects.
Review
Cell Biology
Stepan Cada, Vitezslav Bryja
Summary: This article reviews the activity of Wnt signaling in migratory cells, with a focus on neuronal axon migration, mesenchymal migration models, and local Wnt signaling in amoeboid cells. The review identifies gaps in our current understanding and proposes testable hypotheses for future research based on synthesizing the current observations.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Sayuki Hirano, Yusuke Mii, Guillaume Charras, Tatsuo Michiue
Summary: This study reveals how mechanical signals regulate planar cell polarity (PCP) in the posterior neuroectoderm of Xenopus laevis. It shows that tension in the anterior-posterior direction plays a crucial role in maintaining PCP, and that cells sense tissue stretch indirectly through changes in their shape. Furthermore, the study suggests a cooperative relationship between tissue stretch direction and the diffusion of locally expressed Wnt ligands in establishing PCP.
Article
Cell Biology
Andre Landin Malt, Shaylyn Clancy, Diane Hwang, Alice Liu, Connor Smith, Margaret Smith, Maya Hatley, Christopher Clemens, Xiaowei Lu
Summary: A non-canonical Wnt signaling pathway has been found to promote cochlear outgrowth and coordinate planar polarization of hair cells. Gsk3 beta and Rac1 play crucial roles in this pathway, as shown in genetic rescue experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Anika Boettcher, Maren Buettner, Sophie Tritschler, Michael Sterr, Alexandra Aliluev, Lena Oppenlaender, Ingo Burtscher, Steffen Sass, Martin Irmler, Johannes Beckers, Christoph Ziegenhain, Wolfgang Enard, Andrea C. Schamberger, Fien M. Verhamme, Oliver Eickelberg, Fabian J. Theis, Heiko Lickert
Summary: This study reveals that polarity cues can regulate the fate of intestinal stem cells, challenging previous assumptions about cell lineage hierarchy. The activation of the Wnt/planar cell polarity pathway plays a crucial role in guiding stem cells towards specific lineages, highlighting the importance of niche signals in cell fate determination.
NATURE CELL BIOLOGY
(2021)
Correction
Cell Biology
Anika Bottcher, Maren Buttner, Sophie Tritschler, Michael Sterr, Alexandra Aliluev, Lena Oppenlander, Ingo Burtscher, Steffen Sass, Martin Irmler, Johannes Beckers, Christoph Ziegenhain, Wolfgang Enard, Andrea C. Schamberger, Fien M. Verhamme, Oliver Eickelberg, Fabian J. Theis, Heiko Lickert
Summary: A correction has been published for this paper, the details can be found at the provided link.
NATURE CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jasna Lojk, Janja Marc
Summary: The Wnt signalling pathway is crucial for bone development, homeostasis, and mineral density regulation. It includes both canonical/beta-catenin and non-canonical pathways, which play important roles in bone cell differentiation, maturation, and function, as well as in bone tissue structure regulation. Further research is needed to explore the significance of non-canonical Wnt signalling pathways in bone biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Claudiu V. Giuraniuc, Shabana Zain, Shahmama Ghafoor, Stefan Hoppler
Summary: Researchers used mathematical models to analyze Wnt signaling in early vertebrate embryogenesis, finding different classes of target genes for the zygotic Wnt signaling. A simplified model was able to largely describe the regulatory behavior observed experimentally, suggesting that one super-gene for each class is sufficient.
JOURNAL OF THEORETICAL BIOLOGY
(2022)
Review
Cell Biology
Anne Aalto, Adan Olguin-Olguin, Erez Raz
Summary: This article discusses the migration mechanism of zebrafish primordial germ cells during embryonic development, which involves a bleb-driven amoeboid migration mode controlled by chemical and physical cues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Eudald Pascual-Carreras, Marta Marin-Barba, Sergio Castillo-Lara, Pablo Coronel-Cordoba, Marta Silvia Magri, Grant N. Wheeler, Jose Luis Gomez-Skarmeta, Josep F. Abril, Emili Salo, Teresa Adell
Summary: Any planarian fragment can regenerate the missing head and tail properly by activating the Wnt/beta-catenin signaling pathway, which changes the chromatin accessibility of the wound cells. The determination of missing tissue identity relies on pre-existing tissue. Planarians are perfect organisms for studying this mechanism, as the same group of cells can regenerate a head or a tail depending on the injured body part. The differential activation of the Wnt/beta-catenin signal after amputation specifies anterior or posterior identity. The chromatin accessibility of wound cells shifts according to the polarity of pre-existing tissue in a Wnt/beta-catenin-dependent manner within 12 hours.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Katarzyna Szymanska, Karsten Boldt, Clare Logan, Matthew Adams, Philip A. Robinson, Marius Ueffing, Elton Zeqiraj, Gabrielle Wheway, Colin A. Johnson
Summary: This study provides mechanistic insight into ciliary ubiquitin processing and uncovers UBE2E1 and RNF34 as novel interactants of MKS1. UBE2E1 mediates both regulatory and degradative ubiquitination of MKS1, and phosphorylated beta-catenin processing occurs at the ciliary base.
Review
Biochemistry & Molecular Biology
Jiajia Wang, Xichen Wan, Qihua Le
Summary: SOX9, a member of the SRY-related HMG-box transcription factors, plays a critical role in fetal development and stem cell homeostasis. The interactions between SOX9 and the canonical Wnt signalling pathway are involved in various physiological and pathological processes of stem cells, including organ development, stem cell proliferation, differentiation, and tumorigenesis. This review summarizes the molecular mechanism of cross-interactions between SOX9 and the canonical Wnt signalling pathway, outlines its regulatory effects on homeostasis in different types of stem cells, and explores its potential in translational stem cell therapy.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Maria Jussila, Curtis W. Boswell, Nigel W. Griffiths, Patrick G. Pumputis, Brian Ciruna
Summary: This study utilizes CRISPR/Cas9 genome editing to introduce a fluorescent reporter onto the core PCP component, Vangl2, in zebrafish. Through live imaging, the researchers reveal the authentic regulation of vertebrate PCP during embryogenesis and link PCP defects with idiopathic scoliosis.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Koki Kimura, Shiori Yamamori, Masaharu Hazawa, Jingjing Kobayashi-Sun, Mao Kondo, Richard W. Wong, Isao Kobayashi
Summary: Using a stromal cell line from the zebrafish kidney, researchers found that an inhibitor of canonical Wnt signaling, IWR-1-endo, is a potent regulator of hematopoietic stem cells (HSCs). The stromal cells weakly expressed HSC maintenance factors but highly expressed canonical Wnt ligand genes. Adding IWR-1-endo or inhibiting canonical Wnt signaling increased the number of HSCs.
Review
Cell Biology
Lauren V. Albrecht, Nydia Tejeda-Munoz, Edward M. De Robertis
Summary: Recent investigations have revealed that Wnt signaling can regulate various cell physiological effects, promoting cell growth by mechanisms such as Wnt receptor endocytosis, Wnt-induced protein stabilization, and metabolic changes. A key element is the sequestration of glycogen synthase kinase 3 (GSK3) inside multivesicular bodies and lysosomes, which affects the phosphorylation motifs of GSK3 and protein degradation. These effects are independent of new protein synthesis and contribute to cell growth.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 37
(2021)
Article
Developmental Biology
Maria Jussila, Anne J. Aalto, Maria Sanz Navarro, Vera Shirokova, Anamaria Balic, Aki Kallonen, Takahiro Ohyama, Andrew K. Groves, Marja L. Mikkola, Irma Thesleff
Article
Cell & Tissue Engineering
Vera Shirokova, Leah C. Biggs, Maria Jussila, Takahiro Ohyama, Andrew K. Groves, Marja L. Mikkola
Article
Developmental Biology
Elina Jarvinen, Junko Shimomura-Kuroki, Anamaria Balic, Maria Jussila, Irma Thesleff
Article
Developmental Biology
Emma Juuri, Maria Jussila, Kerstin Seidel, Scott Holmes, Ping Wu, Joy Richman, Kristiina Heikinheimo, Cheng-Ming Chuong, Katrin Arnold, Konrad Hochedlinger, Ophir Klein, Frederic Michon, Irma Thesleff
Article
Developmental Biology
Sayumi Fujimori, Hermann Novak, Martina Weissenboeck, Maria Jussila, Alexandre Goncalves, Rolf Zeller, Jenna Galloway, Irma Thesleff, Christine Hartmann
DEVELOPMENTAL BIOLOGY
(2010)
Article
Anatomy & Morphology
Vera Shirokova, Maria Jussila, Marjo K. Hytonen, Nina Perala, Cord Droegemueller, Tosso Leeb, Hannes Lohi, Kirsi Sainio, Irma Thesleff, Marja L. Mikkola
DEVELOPMENTAL DYNAMICS
(2013)
Article
Cell Biology
Maria Jussila, Xenia Crespo Yanezl, Irma Thesleff
Article
Dentistry, Oral Surgery & Medicine
Emma Juuri, Sanna Isaksson, Maria Jussila, Kristiina Heikinheimo, Irma Thesleff
EUROPEAN JOURNAL OF ORAL SCIENCES
(2013)
Article
Evolutionary Biology
Pauliina M. Munne, Szabolcs Felszeghy, Maria Jussila, Marika Suomalainen, Irma Thesleff, Jukka Jernvalla
EVOLUTION & DEVELOPMENT
(2010)
Article
Cell Biology
Maria Jussila, Irma Thesleff
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2012)
Article
Multidisciplinary Sciences
Maria Jussila, Curtis W. Boswell, Nigel W. Griffiths, Patrick G. Pumputis, Brian Ciruna
Summary: This study utilizes CRISPR/Cas9 genome editing to introduce a fluorescent reporter onto the core PCP component, Vangl2, in zebrafish. Through live imaging, the researchers reveal the authentic regulation of vertebrate PCP during embryogenesis and link PCP defects with idiopathic scoliosis.
NATURE COMMUNICATIONS
(2022)