Review
Cell Biology
Florian Hermans, Lara Hemeryck, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Tooth development relies on interactions between dental epithelium and mesenchyme, regulated by molecular pathways including Wnt, BMP, FGF, and Shh. These signaling modules intertwine during tooth development, outlining specific cell populations' development pathways.Recent studies have uncovered crucial Wnt-Shh interactions during odontogenesis, showing conservation in developing ectodermal appendages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Isabel Mogollon, Jacqueline E. Moustakas-Verho, Minna Niittykoski, Laura Ahtiainen
Summary: Signaling centers, such as the molar initiation knot (IK) and enamel knots (EKs), play crucial roles in regulating tooth development by driving cell dynamics and differentiation processes. The juxtaposition of cells with specific signaling activities is key to the initiation and maturation of these centers, ultimately leading to the formation of tooth structures.
Review
Cell Biology
Ian James Bell, Matthew Sheldon Horn, Terence John Van Raay
Summary: The non-canonical Wnt signaling pathways have different roles in cancer and development, involving calcium and Vangl2. Cells tend to choose non-canonical Wnt signaling over canonical Wnt signaling when presented with a choice, but this occurs rarely in vivo.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hyun-Taek Kim, Paolo Panza, Khrievono Kikhi, Yuko Nakamichi, Ann Atzberger, Stefan Guenther, Clemens Ruppert, Andreas Guenther, Didier Y. R. Stainier
Summary: This study reveals that WNT/RYK signaling plays a regulatory role in lung diseases and highlights the anti-inflammatory modulation of RYK signaling in lung development and homeostasis. These findings provide an animal model for further investigation of the etiology and therapeutic approaches to inflammatory lung diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Max D. Knickmeyer, Juan L. Mateo, Stephan Heermann
Summary: The study demonstrates that BMP plays a significant role in optic chiasm formation and RGC axon pathfinding in zebrafish, potentially through an interaction with midline guidance cues and optic stalk morphogenesis. Induction of BMP4 at different time points results in distinct neurodevelopmental abnormalities, severely affecting RGC axon projections in different ways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Lucie Horakova, Linda Dalecka, Oldrich Zahradnicek, Katerina Lochovska, Herve Lesot, Renata Peterkova, Abigail S. S. Tucker, Maria Hovorakova
Summary: This study investigates the formation of incisor germs in Eda-/- mice through 3D reconstruction, expression analysis, cell lineage tracing experiments, and western blot analysis. The results show that the absence of functional Eda protein has minimal impact on the early expression of Shh in the incisor, but affects the later expression of Shh in the primary enamel knot of the functional tooth. Eda deficiency leads to a smaller region of Shh expression and reduced contribution from Shh descendant cells, resulting in the formation of an abnormal enamel organ and a hypoplastic functional incisor. Eda appears to influence the spatial formation of successional signalling centers during odontogenesis.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Physiology
Xiaoxiao Hu, Chensheng Lin, Ningsheng Ruan, Zhen Huang, Yanding Zhang, Xuefeng Hu
Summary: This study investigates the conservation of the atypical BMP canonical signaling pathway in human odontogenesis and reveals the functional operation of a pathway dependent on Smad1/5/8 but independent of Smad4 in human dental mesenchyme during early tooth development.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Derek Avery, Lais Morandini, Luke S. Sheakley, Arth H. Shah, Loc Bui, Jefferson O. Abaricia, Rene Olivares-Navarrete
Summary: This study investigates the role of canonical Wnt signaling in the response of macrophages to titanium surface properties. The results show that activating canonical Wnt signaling promotes a pro-inflammatory phenotype, while inhibiting it enhances an anti-inflammatory response.
Article
Multidisciplinary Sciences
A. Murashima-Suginami, H. Kiso, Y. Tokita, E. Mihara, Y. Nambu, R. Uozumi, Y. Tabata, K. Bessho, J. Takagi, M. Sugai, K. Takahashi
Summary: Deficiency in USAG-1 leads to supernumerary teeth formation, while antibodies against USAG-1 can accelerate tooth development. Blocking USAG-1 function can alleviate congenital tooth agenesis caused by various genetic abnormalities.
Review
Cell & Tissue Engineering
Xi-Min Hu, Zhi-Xin Li, Dan-Yi Zhang, Yi-Chao Yang, Shen-ao Fu, Zai-Qiu Zhang, Rong-Hua Yang, Kun Xiong
Summary: Hair follicle stem cells (HFSCs) are valuable resources for studying adult stem cells and hair loss therapy, with potential therapeutic targets for skin and hair disorders. Multiple signaling pathways are involved in regulating the fate of HFSCs, providing a better understanding of pathophysiological changes in the HFSC niche.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Ana R. Moshkovsky, Marc W. Kirschner
Summary: Axin is a crucial protein in the canonical Wnt pathway, and its differential regulation allows for tissue-specific functions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Silvia Muccioli, Valentina Brillo, Leonardo Chieregato, Luigi Leanza, Vanessa Checchetto, Roberto Costa
Summary: The Wnt signaling pathway is crucial in embryonic development for controlling cell proliferation, but aberrant activation in human diseases has been observed. Ion channels, known as modulators of important cellular functions, have been widely researched for their influence on Wnt signaling regulation. This review focuses on the links between ion channels and canonical Wnt signaling, discussing their potential roles in human metabolic diseases, neurological disorders, and cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jenaid M. Rees, Victoria A. Sleight, Stephen J. Clark, Tetsuya Nakamura, J. Andrew Gillis, Megan G. Davey
Summary: The anterior-posterior polarity in the gill skeleton of cartilaginous fishes is regulated by the interaction between Wnt and Shh signaling. Wnt signaling restricts the formation of posterior branchial ray cartilages by inhibiting Shh signal transduction, determining the distribution of gill arches. This study highlights the importance of signaling interactions at embryonic tissue boundaries for cell fate determination in vertebrate pharyngeal arches.
Article
Dentistry, Oral Surgery & Medicine
M. Seppala, B. Thivichon-Prince, G. M. Xavier, N. Shaffie, I Sangani, A. A. Birjandi, J. Rooney, J. N. S. Lau, R. Dhaliwal, O. Rossi, M. A. Riaz, D. Stonehouse-Smith, Y. Wang, S. N. Papageorgiou, L. Viriot, M. T. Cobourne
Summary: The mammalian dentition shows wide numerical and morphological variation among different species, with complex molecular signaling interactions controlling odontogenesis. The signaling molecule Sonic hedgehog (Shh) and its coreceptor growth arrest-specific 1 (Gas1) play key roles in this process. Gas1 loss-of-function mutation in mice leads to variation in number, morphology, and size of teeth, with supernumerary teeth arising through vestigial tooth germ proliferation and survival, and Gas1 function essential for regulating tooth number by facilitating Shh signal transduction.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chomchanok Panyarat, Siriruk Nakornchai, Kanoknart Chintakanon, Niramol Leelaadisorn, Worrachet Intachai, Bjorn Olsen, Sissades Tongsima, Ploy Adisornkanj, Chumpol Ngamphiw, Timothy C. Cox, Piranit Kantaputra
Summary: The activation of Wnt/beta-catenin signalling is essential for tooth development. APC, a component of the AXIN-CK1-GSK3 beta-APC beta-catenin destruction complex, regulates Wnt/beta-catenin signalling to establish normal tooth number and position. Loss-of-function mutations in APC result in over-activation of WNT/beta-catenin signalling and can cause familial adenomatous polyposis (FAP) and supernumerary teeth. In this study, rare genetic variants in APC were identified in patients with supernumerary teeth phenotypes, suggesting a contribution of APC to isolated supernumerary dental abnormalities such as mesiodens and isolated supernumerary premolar.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ines Desanlis, Yacine Kherdjemil, Alexandre Mayran, Yasser Bouklouch, Claudia Gentile, Rushikesh Sheth, Rolf Zeller, Jacques Drouin, Marie Kmita
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Virginie Tissieres, Florian Geier, Barbara Kessler, Eckhard Wolf, Rolf Zeller, Javier Lopez-Rios
Article
Cell Biology
Kazuhiko Matsuoka, Latifa Bakiri, Lena Wolff, Markus Linder, Amanda Mikels-Vigdal, Ana Patino-Garcia, Fernando Lecanda, Christine Hartmann, Maria Sibilia, Erwin F. Wagner
Article
Endocrinology & Metabolism
Zhijia Tan, Mingpeng Kong, Songjia Wen, Kwok Yeung Tsang, Ben Niu, Christine Hartmann, Danny Chan, Chi-Chung Hui, Kathryn S. E. Cheah
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Article
Multidisciplinary Sciences
Meng Zhang, Yiwei Lai, Vladislav Krupalnik, Pengcheng Guo, Xiangpeng Guo, Jianguo Zhou, Yan Xu, Zhijun Yu, Longqi Liu, Ao Jiang, Wenjuan Li, Gang Ma, Na Li, Xiuling Fu, Yuan Lv, Mengling Jiang, Muqddas Tariq, Shahzina Kanwal, Hao Liu, Xueting Xu, Hui Zhang, Yinghua Huang, Lulu Wang, Shuhan Chen, Isaac A. Babarinde, Zhiwei Luo, Dongye Wang, Tiantian Zhou, Carl Ward, Minghui He, David P. Ibanez, Yunpan Li, Jiajian Zhou, Jie Yuan, Yayan Feng, Karthik Arumugam, Umberto Di Vicino, Xichen Bao, Guangming Wu, Axel Schambach, Huating Wang, Hao Sun, Fei Gao, Baoming Qin, Andrew P. Hutchins, Bradley W. Doble, Christine Hartmann, Maria Pia Cosma, Yan Qin, Guo-Liang Xu, Runsheng Chen, Giacomo Volpe, Liang Chen, Jacob H. Hanna, Miguel A. Esteban
Article
Biology
Kelsey H. Elliott, Xiaoting Chen, Joseph Salomone, Praneet Chaturvedi, Preston A. Schultz, Sai K. Balchand, Jeffrey D. Servetas, Aimee Zuniga, Rolf Zeller, Brian Gebelein, Matthew T. Weirauch, Kevin A. Peterson, Samantha A. Brugmann
Article
Cell Biology
Stefan Teufel, Petra Koeckemann, Christine Fabritius, Lena Wolff, Jessica Bertrand, Thomas Pap, Christine Hartmann
Summary: Agonists and antagonists of the canonical Wnt signaling pathway have an impact on rheumatoid arthritis, primarily modifying bone loss and soft-tissue inflammation. WNT9a levels are elevated in the pannus tissue of RA patients, suggesting a specific role in TNF-triggered RA.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Jonas Malkmus, Laurene Ramos Martins, Shalu Jhanwar, Bonnie Kircher, Victorio Palacio, Rushikesh Sheth, Francisca Leal, Amandine Duchesne, Javier Lopez-Rios, Kevin A. Peterson, Robert Reinhardt, Koh Onimaru, Martin J. Cohn, Aimee Zuniga, Rolf Zeller
Summary: The study on Grem1 enhancers in the mouse genome revealed the cis-regulatory logic behind the spatio-temporal control of Grem1 during limb bud development, showing that transcript levels are controlled in an additive manner and spatial regulation requires synergistic interactions among multiple enhancers. The research also discovered substantial spatial plasticity in Grem1 regulation, providing insights into the evolution process from fishes to tetrapods.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Julie Gamart, Iros Barozzi, Frederic Laurent, Robert Reinhardt, Laurene Ramos Martins, Thomas Oberholzer, Axel Visel, Rolf Zeller, Aimee Zuniga
Summary: SMAD4 regulates gene expression in early limb buds, primarily mediating BMP signal transduction and playing a crucial role in upregulating target genes in anterior limb bud mesenchyme, while also repressing posterior gene expression. This study reveals opposing trans-regulatory inputs from SHH- and SMAD4-mediated BMP signal transduction during digit patterning and outgrowth in early limb buds.
Article
Multidisciplinary Sciences
Shalu Jhanwar, Jonas Malkmus, Jens Stolte, Olga Romashkina, Aimee Zuniga, Rolf Zeller
Summary: This study compared mouse and chicken limb bud development to investigate the impact of genome evolution on gene regulatory interactions, revealing the importance of spatio-temporal regulation of gene expression in embryonic development. The authors identified shared and species-specific epigenomic and genomic variations, and observed synchrony in chromatin accessibility and gene expression dynamics in mouse forelimb buds, while uncovering species-specific regulatory heterochrony in chicken wing buds. Mapping of transcription factor binding sites and construction of target gene networks revealed both conserved and species-specific interactions, providing a molecular framework for comparative Evo-Devo studies.
NATURE COMMUNICATIONS
(2021)
Article
Endocrinology & Metabolism
Stefan Teufel, Lena Wolff, Ulrich Koenig, Akio Kobayashi, Richard Behringer, Christine Hartmann
Summary: Osteoarthritis (OA) is a common degenerative joint disease with a complex and not fully understood etiology. Recent research has shown the involvement of the Wnt signaling pathway in OA. A study identified the chromosomal location on chromosome 1 linked to the Wnt3a-Wnt9a gene locus as the most significant locus associated with thumb osteoarthritis. The conditional loss of Wnt9a and Wnt4 in mice was found to predispose them to develop spontaneous OA-like changes and increased disease severity.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Benjamin Tosun, Lena Ingeborg Wolff, Astrid Houben, Stephen Nutt, Christine Hartmann
Summary: Osteoclasts play an important role in bone marrow cavity formation, while macrophages appear to have no major role. Studies have shown that mice lacking osteoclasts or macrophages display a delay in bone marrow cavity formation and a lengthening of the zone of hypertrophic chondrocytes during embryonic development.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Maria Jussila, Curtis W. Boswell, Nigel W. Griffiths, Patrick G. Pumputis, Brian Ciruna
Summary: This study utilizes CRISPR/Cas9 genome editing to introduce a fluorescent reporter onto the core PCP component, Vangl2, in zebrafish. Through live imaging, the researchers reveal the authentic regulation of vertebrate PCP during embryogenesis and link PCP defects with idiopathic scoliosis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Angela Morabito, Jonas Malkmus, Anna Pancho, Aimee Zuniga, Rolf Zeller, Rushikesh Sheth
Summary: The protocol introduces a method combining oxidation-mediated autofluorescence reduction and detergent-based tissue permeabilization for RNA-fluorescence in situ hybridization (FISH) on mouse embryonic limb buds.
Article
Cell Biology
Laura Lleras-Forero, Elis Newham, Stefan Teufel, Koichi Kawakami, Christine Hartmann, Chrissy L. Hammond, Robert D. Knight, Stefan Schulte-Merker
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)