Article
Biochemical Research Methods
Jirayu Boonyakida, Doddy Irawan Setyo Utomo, Fahmida Nasrin Soma, Enoch Y. Park
Summary: This study successfully expressed recombinant VP1 of norovirus in silkworms, resulting in the formation of highly purified NoV-LPs. The purified VLPs were shown to be structurally correct, biologically functional, and exhibited potential applications in a vaccine platform. Furthermore, the silkworm-based expression system is known for its robustness, scalability, and cost-effectiveness compared to insect cell systems.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Biotechnology & Applied Microbiology
Doddy Irawan Setyo Utomo, Sabar Pambudi, Enoch Y. Park
Summary: Dengue, a mosquito-borne disease, poses a significant global public health challenge. Researchers have successfully produced dengue virus-like particles using silkworms, which share similar epitopes with the natural dengue virus and elicit a stronger immune response.
Article
Chemistry, Multidisciplinary
Ojodomo J. Achadu, Njemuwa Nwaji, Dongkyu Lee, Jaebeom Lee, Eser M. Akinoglu, Michael Giersig, Enoch Y. Park
Summary: The novel hierarchically porous 3D magnetic molybdenum trioxide-polydopamine-gold functionalized nanosphere (3D mag-MoO3-PDA@Au NS) shows great potential for pathogen detection, overcoming challenges in Raman signal stability and reproducibility, and achieving ultrasensitive detection of SARS-CoV-2 spike protein.
NANOSCALE ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Jian Xu, Takafumi Nakanishi, Tatsuya Kato, Enoch Y. Park
Summary: The Baculovirus expression vector system (BEVS) is an effective protein expression system and has been used to engineer important enzymes and vaccines. The use of fusion tags for protein purification can negatively affect the biological activity of the target protein. This study validated the cleavage activity of the human rhinoviruses (HRV) 3C protease in both Escherichia coli and silkworm-BEVS systems. The findings demonstrate the successful establishment of a tag-free protein engineering platform using a coinfection-based recognition-and-reaction BEVS system.
BIOSCIENCE REPORTS
(2022)
Article
Chemistry, Physical
Oluwasesan Adegoke, Niamh Nic Daeid
Summary: A novel fluorescence aptasensor was developed for ultrasensitive detection of arsenic (As) contamination, with the probe successfully applied in environmental and biological samples. By optimizing the probe composition, selective and quantitative analysis of As(III) was achieved with high sensitivity.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Chemistry, Physical
Oluwasesan Adegoke, Svetlana Zolotovskaya, Amin Abdolvand, Niamh Nic Daeid
Summary: In this study, a novel fluorescence sensor for levamisole was developed using AuZnFeSeS alloyed quantum dots-molecularly imprinted polymer nano composite. Under optimal conditions, quantitative detection of levamisole with high selectivity was achieved.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Biochemistry & Molecular Biology
Jirayu Boonyakida, Indra Memdi Khoris, Fahmida Nasrin, Enoch Y. Park
Summary: This study aimed to develop a modular display platform for protein decoration on norovirus-like particles (NoV-LPs) by combining the NoVLP scaffold with the SpyTag/SpyCatcher bioconjugation system. Results showed that adding an extension linker increased the conjugation efficiency and level of protein decoration on the NoV-LPs. This study provides a strategy for enhancing the conjugation efficiency of NoV-LP and demonstrates the platform's utility for developing vaccines or functional nanoparticles.
Article
Chemistry, Analytical
Akhilesh Babu Ganganboina, Indra Memdi Khoris, Akinori Konno, Tian-Cheng Li, Akihiro Okamoto, Enoch Y. Park
Summary: This article describes the design and construction of a visible light-driven photoelectrochemical (PEC) device based on a CdSe-Co3O4@TiO2 nanoflower. The device demonstrated ultrasensitive detection of viruses including hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complex lysate solution. The enhanced photocurrent response of the PEC device can be attributed to the CdSe quantum dot sensitization effect and strong visible light absorption, improving system stability and energy conversion efficiency. The device achieved considerable sensitivity, good specificity, and acceptable stability, indicating its potential for affordable and portable biosensing capabilities.
Article
Biochemical Research Methods
Fahmida Nasrin, Indra Memdi Khoris, Ankan Dutta Chowdhury, Sjakurrizal El Muttaqein, Enoch Y. Park
Summary: To prevent viral diseases, the development of a disposable, rapid, and convenient biosensor with high sensitivity and reliability is highly desired. In this study, a practical impedimetric biosensor has been created on a disposable electrode for the detection of two mosquito-borne viruses. The biosensor fabrication involves the formation of a conductive film, physical binding of gold nanoparticles-polyaniline, and biofunctionalization. The disposable electrode has been designed to detect Chikungunya virus and Zika virus separately with a wide linear range and low detection limits.
BIOTECHNOLOGY JOURNAL
(2023)
Correction
Chemistry, Physical
Oluwasesan Adegoke, Niamh Nic Daeid
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Engineering, Electrical & Electronic
Oluwasesan Adegoke, M. Laura Nsuamani, Niamh Nic Daeid
Summary: This study reports the development of a novel AuZnCeSeS quantum dots (QDs)-molecular imprinted polymer (MIP) nanocomposite fluorescent probe for methamphetamine (METH) recognition. The probe showed excellent selectivity and sensitivity in detecting METH.
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2023)
Article
Chemistry, Analytical
Oluwasesan Adegoke, Kayode Oyinlola, Ojodomo J. Achadu, Zhugen Yang
Summary: In this study, a novel metal-enhanced fluorescence (MEF) and electrochemical biosensor was developed for the detection of SARS-CoV-2 spike (S) protein. The biosensor utilized SiO2-coated Si-doped ZnSeS quantum dots (QDs) and aptamer-molecular beacon (Apta-MB) as the sensing elements, and cationic gold nanoparticles (AuNPs) as the signal amplifiers. Under optimized experimental conditions, the biosensor demonstrated enhanced sensitivity in detecting the SARS-CoV-2 S protein. The detection was successfully performed in saliva samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Marina Ikegaya, Enoch Y. Park, Takatsugu Miyazaki
Summary: Glycoside hydrolase family 31 (GH31) contains a-glycoside hydrolases with different substrate specificities involved in various physiological functions. This study characterized two a-galactosidases, BsGH31_19 and FpGH31_19, and found that they showed high substrate specificity against a-(1?4)-linkages in a-(1?4)-galactobiose and globotriose, unlike other reported GH31 a-galactosidases PsGal31A and MYORG. Structural analyses revealed differences in amino acid residues and dimer interfaces between BsGH31_19 and FpGH31_19 compared to PsGal31A and MYORG, providing insights into the molecular diversity and evolutionary relationships of GH31 a-galactosidases and other a-galactosidase families.
Article
Chemistry, Physical
Oluwasesan Adegoke, Niamh Nic Daeid
Summary: This work presents the development of a novel rapid, ultrasensitive, and selective immunofluorescence nanobiosensor probe for detecting MDMA in urine samples. The nanoprobe utilizes nanomaterials conjugated with anti-MDMA antibodies to capture the target drug, allowing for early and accurate detection. Experimental analysis shows successful detection of MDMA using this nanoprobe with satisfactory recoveries in urine samples.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Review
Chemistry, Analytical
Sjaikhurrizal El Muttaqien, Indra Memdi Khoris, Sabar Pambudi, Enoch Y. Y. Park
Summary: In the past few decades, nanomaterials have been explored in the sensing research field. However, less attention has been given to their application in biosensors for pathogenic virus detection. This review focuses on 3D nanospheres, a type of nanostructured material, and their extensive use in various sensing techniques. Signal amplification strategies using different types of nanospheres are discussed, along with challenges related to toxicity and commercialization in the development of nanosphere-based biosensors.
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
