Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

Inhibition of phospho-MurNAc-pentapeptide translocase (MraY) by nucleoside natural product antibiotics, bacteriophage ϕX174 lysis protein E, and cationic antibacterial peptides

(2016) Timothy D.H. Bugg et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Structural insights into inhibition of lipid I production in bacterial cell wall synthesis

(2016) Ben C. Chung et al.   NATURE

High-resolution mass spectrometry of small molecules bound to membrane proteins

(2016) Joseph Gault et al.   NATURE METHODS

Nanodiscs for structural and functional studies of membrane proteins

(2016) Ilia G Denisov et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis of lipoprotein signal peptidase II action and inhibition by the antibiotic globomycin

(2016) L. Vogeley et al.   SCIENCE

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

(2016) Aisha Laguerre et al.   STRUCTURE

Nanodiscs for structural and functional studies of membrane proteins

(2016) Ilia G Denisov et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Probing the Lipid Annular Belt by Gas-Phase Dissociation of Membrane Proteins in Nanodiscs

(2015) Michael T. Marty et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Membrane protein production inEscherichia colicell-free lysates

(2015) Erik Henrich et al.   FEBS LETTERS

Lipid Requirements for the Enzymatic Activity of MraY Translocases andin VitroReconstitution of the Lipid II Synthesis Pathway

(2015) Erik Henrich et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR

(2015) Jakob Maciejko et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A subset of annular lipids is linked to the flippase activity of an ABC transporter

(2015) Chérine Bechara et al.   Nature Chemistry

Resting state of the human proton channel dimer in a lipid bilayer

(2015) Qufei Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The emerging age of cell-free synthetic biology

(2014) Mark Thomas Smith et al.   FEBS LETTERS

Membrane proteins bind lipids selectively to modulate their structure and function

(2014) Arthur Laganowsky et al.   NATURE

Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins

(2013) Franz Hagn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Detergent-free mass spectrometry of membrane protein complexes

(2013) Jonathan T S Hopper et al.   NATURE METHODS

Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis

(2013) B. C. Chung et al.   SCIENCE

Massign: An Assignment Strategy for Maximizing Information from the Mass Spectra of Heterogeneous Protein Assemblies

(2012) Nina Morgner et al.   ANALYTICAL CHEMISTRY

Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes

(2012) Michael T. Marty et al.   ANALYTICAL CHEMISTRY

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase

(2012) Christian Roos et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Solution NMR Structure of Proteorhodopsin

(2011) Sina Reckel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Advances in the Mass Spectrometry of Membrane Proteins: From Individual Proteins to Intact Complexes

(2011) Nelson P. Barrera et al.   Annual Review of Biochemistry

Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes

(2011) Yi Ma et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

Antiparallel EmrE exports drugs by exchanging between asymmetric structures

(2011) Emma A. Morrison et al.   NATURE

Positioning cell wall synthetic complexes by the bacterial morphogenetic proteins MreB and MreD

(2010) Courtney L. White et al.   MOLECULAR MICROBIOLOGY

Membrane protein assembly into Nanodiscs

(2009) Timothy H. Bayburt et al.   FEBS LETTERS

Three-dimensional Structure of A1A0ATP Synthase from the Hyperthermophilic ArchaeonPyrococcus furiosusby Electron Microscopy

(2009) Janet Vonck et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell-free expression and stable isotope labelling strategies for membrane proteins

(2009) Solmaz Sobhanifar et al.   JOURNAL OF BIOMOLECULAR NMR

pH-, temperature- and ion-dependent oligomerization ofSulfolobus solfataricusrecombinant amidase: a study with site-specific mutants

(2009) Laura Politi et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

Lipids and membrane protein structures

(2008) C HUNTE et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Micelles Protect Membrane Complexes from Solution to Vacuum

(2008) N. P. Barrera et al.   SCIENCE

Folding and Assembly of Proteorhodopsin

(2007) Adriana L. Klyszejko et al.   JOURNAL OF MOLECULAR BIOLOGY