Article
Cell Biology
Bruno Jorge de Andrade Silva, Tamiris Lameira Bittencourt, Thyago Leal-Calvo, Mayara Abud Mendes, Rhana Berto da Silva Prata, Mayara Garcia de Mattos Barbosa, Priscila Ribeiro Andrade, Suzana Corte-Real, Gilberto Marcelo Sperandio da Silva, Milton Ozorio Moraes, Euzenir Nunes Sarno, Roberta Olmo Pinheiro
Summary: The study suggests that IFN-gamma may induce a TPR-mediated autophagy transcriptional program, similar to that observed in skin cells during T1R, and involves the production of IL-15, indicating the potential involvement of this cytokine in the pathogenesis of T1R.
Article
Cell Biology
Carles Recasens-Alvarez, Cyrille Alexandre, Joanna Kirkpatrick, Hisashi Nojima, David J. Huels, Ambrosius P. Snijders, Jean-Paul Vincent
Summary: Ribosomes are essential molecular machines for protein synthesis, and deficiencies in ribosomal proteins can lead to proteotoxic stress and cell apoptosis. Modulating the integrated stress response and autophagy can affect the severity of ribosomal protein deficiency-induced harm, suggesting potential cytoprotective strategies for ribosomopathies.
NATURE CELL BIOLOGY
(2021)
Article
Medicine, General & Internal
Changqing Ma, Chad E. Storer, Uma Chandran, William A. LaFramboise, Patricia Petrosko, Madison Frank, Douglas J. Hartman, Liron Pantanowitz, Talin Haritunians, Richard D. Head, Ta-Chiang Liu
Summary: Individuals with the ATG16L1 T300A genotype in gastric carcinoma show suppressed EGFR and PPAR pathways, increased tumor apoptosis, and improved overall survival. Genotyping gastric cancer patients may offer additional insights for clinical stratification.
Article
Medicine, Research & Experimental
Xiaoxia Bai, Jinshi Ran, Xianlei Zhao, Yun Liang, Xiaohang Yang, Yongmei Xi
Summary: Mother-to-child transmission is the major cause of chronic HBV infection, and this study suggests that HBV may exploit an unconventional protein secretion pathway dependent on autophagy to complete intracellular transport, potentially leading to intrauterine transmission via AnxA2-S100A10-mediated exocytosis in trophoblasts.
LABORATORY INVESTIGATION
(2022)
Article
Medicine, Research & Experimental
Hamza Malik Okuyan, Serdar Dogan, Tayibe Bal, Mehmet Cabalak
Summary: The study reveals that serum BECN1 levels are elevated in COVID-19 patients compared to healthy controls, with significantly higher concentrations in severe cases. BECN1 levels show significant positive correlations with coagulation markers, inflammation markers, and biochemical markers.
Article
Multidisciplinary Sciences
Yun Guo, Fei Gao, Xin Wang, Zhenzhen Pan, Qian Wang, Shiyao Xu, Shanshan Pan, Ling Li, Deyu Zhao, Jun Qian
Summary: The study found that the increase in spontaneous NETosis is time-dependent, and autophagy plays a crucial role in NET formation, with trehalose treatment significantly increasing NET formation but rapamycin not affecting it.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Wanglong Zheng, Nengwen Xia, Jiajia Zhang, Nanhua Chen, Francois Meurens, Zongping Liu, Jianzhong Zhu
Summary: The cGAS-STING pathway is involved in triggering both canonical and non-canonical autophagy, which plays crucial roles in balancing innate immune responses, maintaining intracellular environmental homeostasis, alleviating liver injury, and limiting tumor growth and transformation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Tao Wang, Rui Luo, Jing Zhang, Zhanhao Lu, Lian-Feng Li, Yong-Hui Zheng, Li Pan, Jing Lan, Huanjie Zhai, Shujian Huang, Yuan Sun, Hua-Ji Qiu
Summary: In this study, MGF300-2R was identified as a key factor for replication and pathogenicity of African swine fever virus (ASFV). MGF300-2R promotes the K27-linked ubiquitination of IKKα and IKKβ for TOLLIP-dependent autophagic degradation, suppressing the activation of the NF-κB signaling pathway. These findings highlight the relevance of the inflammatory response in ASFV virulence and suggest MGF300-2R as a potential target for vaccine development.
Article
Oncology
Katrin Bankov, Falko Schulze, Steffen Gretser, Henning Reis, Nada Abedin, Fabian Finkelmeier, Jorg Trojan, Stefan Zeuzem, Andreas A. Schnitzbauer, Dirk Walter, Peter J. Wild, Maximilian N. Kinzler
Summary: Limited data on the prevalence and impact of autophagy in primary cholangiocarcinoma (CCA) tissue have been reported. This study found that active autophagy was present in a minority of CCA patients and its absence was associated with impaired overall survival. Furthermore, patients with active autophagy exhibited higher levels of pan-acetylation. These findings highlight the importance of active autophagy as a prognostic marker and potential therapeutic target in CCA patients.
Article
Medicine, Research & Experimental
Ellesandra C. Noye, Siroon Bekkering, Albert P. Limawan, Maria U. Nguyen, Lisa K. Widiasmoko, Hui Lu, Salvatore Pepe, Michael M. Cheung, Trevelyan R. Menheniott, Megan J. Wallace, Timothy J. Moss, David P. Burgner, Kirsty R. Short
Summary: The study found that postnatal LPS exposure in atherosclerosis-prone mice resulted in a trained phenotype, which was not enhanced by prenatal inflammation. Additionally, combined prenatal and postnatal LPS exposure was associated with a reduction in populations of myeloid progenitor cells in the bone marrow.
Article
Engineering, Biomedical
Rou Xie, Shaobo Ruan, Jiaqi Liu, Lin Qin, Chuanyao Yang, Fan Tong, Ting Lei, Maxim Shevtsov, Huile Gao, Yi Qin
Summary: The study developed a furin-responsive aggregated nanoplatform to address the challenges of insufficient drug accumulation and chemoresistance in cancer chemotherapy. By loading doxorubicin and hydroxychloroquine, the platform enhanced tumor cell sensitivity to chemotherapy drugs and exerted anti-tumor effects through macrophage polarization.
Article
Biotechnology & Applied Microbiology
Michelle Miranda Lopes Falcao, Johelle Santana Passos-Soares, Paulo Roberto Lima Machado, Isaac Suzart Gomes-Filho, Lucas Pedreira de Carvalho, Elisangela Jesus de Campos, Mariana Costa Calheira, Patricia Mares de Miranda, Rebeca Pereira Bulhosa Santos, Jose Tadeu Raynal Rocha Filho, Antonio Pedro Froes de Farias, Taiana Peixoto, Roberto Meyer Nascimento, Gregory John Seymour, Soraya Castro Trindade
Summary: The aim of the study was to evaluate the association between salivary anti-Porphyromonas gingivalis IgA antibodies and the leprosy reaction. Salivary levels of anti-P. gingivalis IgA antibodies were measured in individuals diagnosed with leprosy, and a positive association with the leprosy reaction was found. Individuals with high levels of salivary anti-P. gingivalis IgA were approximately twice as likely to develop the leprosy reaction.
Article
Food Science & Technology
Yilu Xu, Lancheng Wei, Shen Tang, Qianqian Shi, Bin Wu, Xiaobo Yang, Yunfeng Zou, Xinhang Wang, Qingqing Ao, Ling Meng, Xuejing Wei, Ning Zhang, Yunqing Li, Chunhua Lan, Muting Chen, Xiyi Li, Cailing Lu
Summary: This study found that upregulating the methylation level of PP2Ac can ameliorate autophagy dysfunction caused by manganese in N2a cells, reducing cytotoxicity and oxidative stress.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Immunology
Godfrey S. Temba, Vesla Kullaya, Tal Pecht, Blandina T. Mmbaga, Anna C. Aschenbrenner, Thomas Ulas, Gibson Kibiki, Furaha Lyamuya, Collins K. Boahen, Vinod Kumar, Leo A. B. Joosten, Joachim L. Schultze, Andre J. van der Ven, Mihai G. Netea, Quirijn de Mast
Summary: The study on healthy Tanzanians found that urban living is associated with a pro-inflammatory immune phenotype compared to rural living, with differences in food-derived and endogenous circulating metabolites identified. Urban dwellers showed reprogramming of innate immune cells inducing higher tumor necrosis factor production, indicating shifts towards an inflammatory phenotype associated with urban lifestyle. This suggests potential implications for disease epidemiology in Sub-Saharan African countries undergoing rapid urbanization.
Review
Pharmacology & Pharmacy
Siya Kamat, Madhuree Kumari
Summary: Chloroquine and its derivatives have been long used to treat malaria and autoimmune diseases, but their potential in fighting against SARS-CoV-2 requires further research and evaluation. The drug has multiple mechanisms of action, including inhibiting viral replication and cytokine storm, but large-scale, unbiased clinical trials are needed.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Immunology
Tamiris Lameira Bittencourt, Rhana Berto da Silva Prata, Bruno Jorge de Andrade Silva, Mayara Garcia de Mattos Barbosa, Margareth Pretti Dalcolmo, Roberta Olmo Pinheiro
Summary: Pathogenic mycobacteria can manipulate the immune system in the skin, leading to different clinical presentations and societal stigma. Understanding the immunopathogenic mechanisms is crucial for developing new therapeutic strategies, with a focus on the activation of autophagy as a potential target for drug development against cutaneous mycobacterial infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Otto Castro Nogueira, Mariana Gandini, Natasha Cabral, Vilma de Figueiredo, Rodrigo Nunes Rodrigues-da-Silva, Josue da Costa Lima-Junior, Roberta Olmo Pinheiro, Geraldo Moura Batista Pereira, Maria Cristina Vidal Pessolani, Cristiana Santos de Macedo
Summary: This study analyzed the profile of B cell subpopulations in leprosy patients and found that Mycobacterium leprae infection could alter the proportions of B cell subpopulations. The modulation in B cell subpopulations was associated with an increase in total IgG levels and the patient's clinical condition, indicating that circulating B cells may be involved in modulating the immune response to M. leprae in patients with leprosy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Bruno Jorge de Andrade Silva, Tamiris Lameira Bittencourt, Thyago Leal-Calvo, Mayara Abud Mendes, Rhana Berto da Silva Prata, Mayara Garcia de Mattos Barbosa, Priscila Ribeiro Andrade, Suzana Corte-Real, Gilberto Marcelo Sperandio da Silva, Milton Ozorio Moraes, Euzenir Nunes Sarno, Roberta Olmo Pinheiro
Summary: The study suggests that IFN-gamma may induce a TPR-mediated autophagy transcriptional program, similar to that observed in skin cells during T1R, and involves the production of IL-15, indicating the potential involvement of this cytokine in the pathogenesis of T1R.
Article
Medicine, General & Internal
Kyssia Karen de Paiva e Silva, Erick Esteves de Oliveira, Carolina Martins Moreira Elias, Ingrid Estevam Pereira, Roberta Olmo Pinheiro, Euzenir Nunes Sarno, Malcolm Scott Duthie, Henrique Couto Teixeira
Summary: This study found that multibacillary leprosy patients had elevated serum IgA responses, while paucibacillary patients showed increased IgA reactivity mainly to NDO-HSA. Household contacts also exhibited higher IgA responses to NDO-HSA, suggesting that measuring serum IgA against NDO-HSA could be a useful tool for diagnosis and classification of leprosy.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Marcia Jardim, Robson T. Vital, Ximena Illarramendi, Mariana Hacker, Beatriz Junqueira, Izabela J. R. Pitta, Roberta O. Pinheiro, Euzenir N. Sarno
Summary: The diagnosis of pure neural leprosy is challenging as patients share characteristics with other pathologies, such as ulnar compression. This study identified ulnar nerve conduction characteristics and putative markers to aid in differential diagnosis. Clinical signs and electrodiagnostic criteria can help determine the best course of treatment for patients with ulnar neuropathy.
Editorial Material
Immunology
Fatima Conceicao-Silva, Fernanda N. Morgado, Roberta O. Pinheiro, Fabienne Tacchini-Cottier
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Geriatrics & Gerontology
Julia T. Oliveira, Vanja Dakic, Gabriela Vitoria, Carolina da S. G. Pedrosa, Mayara Mendes, Luiz Guilherme H. S. Aragao, Thyago R. Cardim-Pires, Damien Lelievre, Daniel Rodrigues Furtado, Roberta O. Pinheiro, Debora Foguel, Lionel Breton, Charbel Bouez, Rodrigo De Vecchi, Marilia Zaluar P. Guimaraes, Stevens Rehen
Summary: This study reveals that oligomeric alpha-Synuclein (O alpha-Syn) induces inflammation and degeneration in the skin, leading to a decrease in keratinocyte proliferation and thickness of the epidermis. These findings shed light on the potential role of O alpha-Syn in the aging process of the skin.
NEUROBIOLOGY OF AGING
(2022)
Article
Multidisciplinary Sciences
Rhana Berto da Silva Prata, Mayara Abud Mendes, Vinicius Cardoso Soares, Jaqueline Franca-Costa, Anna Maria Sales, Nadia Cristina Duppre, Valeria de Matos Borges, Tatiana Pereira da Silva, Patricia Torres Bozza, Marcelo Torres Bozza, Euzenir Nunes Sarno, Milton Ozorio Moraes, Gilberto Marcelo Sperandio da Silva, Roberta Olmo Pinheiro
Summary: Household contacts of leprosy patients have a higher risk of developing the disease, with genes, antibodies, and arginase activity playing important roles. Evaluating arginase activity can be a predictive marker for deciding whether contacts should receive preventive treatment in leprosy control programs.
SCIENTIFIC REPORTS
(2022)
Review
Immunology
Jessica Araujo da Paixao de Oliveira, Mariana Martins de Athaide, Atta Ur Rahman, Mayara Garciade de Mattos Barbosa, Marcia Maria Jardim, Milton Ozorio Moraes, Roberta Olmo Pinheiro
Summary: The review discusses how modulation of the kynurenine pathway (KP) may help control the damage to peripheral nerves in leprosy and COVID-19, and speculates its role during co-infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Thiara Manuele Alves de Souza, Caroline Fernandes-Santos, Jessica Araujo da Paixao de Oliveira, Larissa Cristina Teixeira Tome, Victor Edgar Fiestas-Solorzano, Priscila Conrado Guerra Nunes, Gabriel Macedo Costa Guimaraes, Juan Camilo Sanchez-Arcila, Iury Amancio Paiva, Luis Jose de Souza, Paulo Vieira Damasco, Valber da Silva Frutuoso, Manoela Heringer, Luzia Maria De Oliveira-Pinto, Roberta Olmo Pinheiro, Flavia Barreto dos Santos, Elzinandes Leal de Azeredo
Summary: This study evaluated the activity of IDO-1 and the patterns of cytokines/chemokines in CHIKV patients. It was found that IDO-1 activity increased during the early acute phase of infection and decreased in the chronic phase. In the acute phase, concentrations of TNF-alpha, IL-6, IFN-gamma, CCL2/MCP-1, and CXCL10/IP-10 were increased. In the chronic phase, concentrations of CCL4/MIP-1 beta were increased, especially in patients with arthritis.
Article
Medicine, General & Internal
Beatriz Junqueira de Souza, Mayara Abud Mendes, Gilberto Marcelo Sperandio da Silva, Patricia Sammarco-Rosa, Milton Ozorio de Moraes, Marcia Rodrigues Jardim, Euzenir Nunes Sarno, Roberto Olmo Pinheiro, Bruno Siqueira Mietto
Summary: Peripheral neuropathy is the main cause of physical disability in leprosy patients. The interaction between viable and dead Mycobacterium leprae and Schwann cells plays a crucial role in the development of leprosy and neuropathy. Understanding the gene regulation and response of Schwann cells to viable and dead bacteria is essential for elucidating the progress and pathology of leprosy.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Gilberto Marcelo Sperandio da Silva, Mauro Felippe Felix Mediano, Michele Ferreira Murgel, Patricia Mello Andrade, Marcelo Teixeira de Holanda, Claudia Maria Valete Rosalino, Andrea Silvestre de Sousa, Fernanda de Souza Nogueira Sardinha Mendes, Roberta Olmo Pinheiro, Valdilea Goncalves Veloso, Roberto Magalhaes Saraiva, Alejandro Marcel Hasslocher-Moreno
Summary: The COVID-19 infection had a significant impact on the mortality risk of in-hospital CD patients at INI-Fiocruz, accounting for one-third of deaths overall.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Tran H. Do, Feiyang Ma, Priscila R. Andrade, Rosane Teles, Bruno J. de Andrade Silva, Chanyue Hu, Alejandro Espinoza, Jer-En Hsu, Chun-Seok Cho, Myungjin Kim, Jingyue Xi, Xianying Xing, Olesya Plazyo, Lam C. Tsoi, Carol Cheng, Jenny Kim, Bryan D. Bryson, Alan M. O'Neill, Marco Colonna, Johann E. Gudjonsson, Eynav Klechevsky, Jun Hee Lee, Richard L. Gallo, Barry R. Bloom, Matteo Pellegrini, Robert L. Modlin
Summary: Acne, a common skin condition affecting 1 in 10 people globally, is characterized by disfiguring facial lesions. This study explored the mechanisms underlying the disease using single-cell and spatial RNA sequencing, as well as ultrahigh-resolution Seq-Scope analysis. The researchers identified TREM2 macrophages expressing lipid metabolism and proinflammatory genes near the hair follicle epithelium, which expressed squalene epoxidase. Through in vitro experiments, it was discovered that squalene induced differentiation of TREM2 macrophages, impairing their ability to kill Cutibacterium acnes. Additionally, the addition of squalene to macrophages inhibited oxidative enzymes and scavenged oxygen free radicals, providing insights into the efficacy of benzoyl peroxide in acne treatment. These findings contribute to our understanding of the role of TREM2 macrophages and unsaturated lipids in the pathogenesis of acne, as well as their similarity to atherosclerosis.
SCIENCE IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Thabatta Leal Silveira Andrezo Rosa, Mayara Abud Mendes, Natasha Ribeiro Cardoso Linhares, Thais Fernanda Rodrigues, Andre Alves Dias, Thyago Leal-Calvo, Mariana Gandini, Helen Ferreira, Fabricio da Mota Ramalho Costa, Anna Maria Sales, Thais Porto Amadeu, Veronica Schmitz, Roberta Olmo Pinheiro, Luciana Silva Rodrigues, Milton Ozorio Moraes, Maria Cristina Vidal Pessolani
Summary: This study investigated the activation of the type I interferon (IFN-I) pathway in leprosy patients with erythema nodosum leprosum (ENL). The results showed higher activation of the IFN-I pathway in ENL patients, which was reduced during thalidomide treatment. Peripheral pDCs were found to be involved in the production of IFN-I during ENL. These findings contribute to the identification of biomarkers for early diagnosis and the discovery of new therapeutic targets for the management of ENL reactions.
FRONTIERS IN MEDICINE
(2022)
Review
Parasitology
Carmen Phang Romero, Rodolfo Castro, Pedro Emmanuel A. do Brasil, Daniella R. Pereira, Roberta Olmo Pinheiro, Cristiana M. Toscano, Maria Regina Fernandes de Oliveira
Summary: This systematic review evaluates the accuracy of rapid point-of-care (POC) tests for diagnosis of leprosy. The results show that these tests have high sensitivity and specificity in multibacillary and paucibacillary cases.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2022)