Article
Chemistry, Analytical
Kangkang Wang, Tingting Yao, Jiayu Xue, Yanqiu Guo, Xiaowei Xu
Summary: Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is closely related to various pathological and physiological processes in living organisms. Excessive H2O2 can lead to diseases such as cancer, diabetes, and cardiovascular diseases, necessitating the detection of H2O2 in living cells. This study designed a new fluorescent probe that effectively detects H2O2 with high selectivity and measures cellular ROS levels, providing a potential monitoring tool for various diseases caused by H2O2 excess.
Article
Chemistry, Inorganic & Nuclear
Shuyue Ma, Kang-Nan Wang, Miaomiao Xing, Fei Feng, Qiling Pan, Duxia Cao
Summary: A coumarin derivative CBE fluorescent chemosensor was developed for efficient detecting H2O2 in living cells through boronic ester elimination. The probe can specifically recognize H2O2 with high sensitivity and selectivity, providing a rapid response and detection within 16 minutes.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Jia Kong, Jinyao Hu, Jia Li, Jiaxing Zhang, Yuhe Shen, Tianli Yue, Xihui Shen, Yuefei Wang, Zhonghong Li, Yinqiang Xia
Summary: Reconstructing miniaturized peptides to mimic specific protein functions is appealing but challenging. In this study, we propose a practical approach to construct a mimetic peptide of redox-sensitive green fluorescent protein-2 (roGFP2) by rethreading aromatic residues adjacent to the chromophore fragment. By fine-tuning the residues, we designed a mini tetrapeptide (Cys-Phe-Phe-His) that acts as a hydrogen peroxide sensor using ratiometric fluorescence. The roGFP2 mimetic tetrapeptide, with its assembly-induced emissions, is biocompatible, photostable, and exhibits competitive fluorescent properties compared to roGFP2. We demonstrated the ability of the ratiometric tetrapeptide to sense hydrogen peroxide in acidic chambers, providing a promising approach for artificially designing miniaturized peptides with desired functions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jing Li, Xueqing Ma, Wei Yang, Chao Guo, Jingying Zhai, Xiaojiang Xie
Summary: This article investigates a sulfite-sensitive nanoreactor that exhibits high selectivity, rapid response, excellent detection limit, and very high sensitivity. The nanoreactor shows negligible toxicity in cellular studies and can be used to observe the responses of different types of cells to external stimuli.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Ling Wang, Xuben Hou, Hao Fang, Xinying Yang
Summary: Fluorescent probes have been widely used as important tools for detecting H2O2 to reflect the physiological and pathological conditions of biological systems, with boronate ester and boronic acid groups serving as sensitive responsive parts in these probes. This review summarizes fluorescent probes based on boronate ester/boronic acid groups reported from 2012 to 2020, and classifies the fluorophores into six categories to elaborate the design strategy and systematic performance comprehensively.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Environmental Sciences
Zhuohang Li, Liyan Xiao, Xiaoqian Sun, Chenyao Luo, Rencheng Li, Wenbo Zhang, Zicheng Wang, Haibin Xiao, Wei Shu
Summary: A novel ESIPT ratio fluorescent probe (probe 1) has been proposed for detecting H2O2 in water environment and biosystems. Probe 1 exhibits short response time, high sensitivity, and a large Stokes shift to H2O2. It has been successfully used to detect H2O2 in actual water samples as well as endogenous and exogenous H2O2 in living cells, with wide applications in environmental and biological systems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Soumen Ghosh, Mohd Avais, Subrata Chattopadhyay
Summary: A responsive fluorescent polyaminoamide nanogel (NANO-PAMAM) was synthesized via a water-based reaction, and then it was covalently linked with doxorubicin to form a ratiometric nanosensor for temperature and pH sensing. The biocompatibility of the nanogel and nanosensor was confirmed by MTT assay.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Lan Guo, Shuai Chen, Yong-Liang Yu, Jian-Hua Wang
Summary: POCT with advantages of simplicity, rapidity, portability, and low-cost is crucial for improving healthcare, while quantitative POCT of multiplexed biomarkers poses a challenge. A smartphone optical device has been designed for accurate testing of glucose and cholesterol using a ratiometric fluorescent sensor, showing great potential for portable detection of numerous targets in personalized healthcare and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Polymer Science
Yeoju Yoon, Seonyoung Jo, Dong Hoon Lee, Taek Seung Lee
Summary: A conjugated polymer containing the o-AN group was synthesized and showed distinct color and fluorescence in solution. The polymer demonstrated excellent F-ion detection ability and ratiometric color change properties.
Article
Chemistry, Inorganic & Nuclear
Xinyue Zhu, Xinru Wu, Jiaojiao Liu, Lina Zou, Baoxian Ye, Gaiping Li
Summary: A dual-emission lanthanide coordination polymer was synthesized, which enabled the development of a simple, highly selective, and sensitive ratiometric glucose assay.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Tuanwei Li, Kaili Cao, Xiaohu Yang, Yongyang Liu, Xingyu Wang, Feng Wu, Guangcun Chen, Qiangbin Wang
Summary: This study presents a single-component NIR-II ratiometric molecular nanoprobe (LC-1250 NP) for early monitoring of gastrointestinal diseases via oral administration. LC-1250 NP shows stable fluorescence in the 1250 nm long-pass channel before and after disease detection, but exhibits enhanced fluorescence in the 1150 nm short-pass channel in the diseased gastrointestinal environment due to the activation of LC-1250 molecules by H2O2. The ratio of fluorescence signals (F1150SP/F1250LP) linearly increases with the concentration of H2O2, providing accurate mapping of the diseased areas and reliable evaluation for the progress of gastroenteritis.
Article
Chemistry, Multidisciplinary
Rongrong Zhou, Qiyao Peng, Dan Wan, Chao Yu, Yuan Zhang, Yi Hou, Quan Luo, Xiong Li, Shuihan Zhang, Lin Xie, Pinghua Ou, Yongbo Peng
Summary: A new lysosome-targeting fluorescent probe NPT-H2O2 was developed for ratiometric detection and imaging of H2O2, especially in lysosomes and inflammation models. The probe exhibited rapid response, high selectivity and sensitivity towards H2O2, along with low cytotoxicity. The results suggest that this probe can serve as a powerful tool to investigate the physiological and pathological relationship between inflammation and lysosomal H2O2.
Article
Chemistry, Applied
Zhongyong Xu, Conghui Zeng, Yutian Zhao, Mei Zhou, Taoyuze Lv, Chao Song, Tianyi Qin, Lei Wang, Bin Liu, Xiaojun Peng
Summary: This study reports a novel supramolecular strategy that converts the typical on-off response to H2O2 into a ratiometric response, enabling on-site detection of H2O2 with a smartphone-based miniaturized testing system. The method shows acceptable sensitivity, good anti-interference ability, and desirable accuracy compared to standard methods, and can be used to detect H2O2 residue in commercial milk samples with portable testing apparatuses.
Article
Chemistry, Physical
Liqing Li, Minghao Zheng, Xiaoyang Yan, Hui Huang, Shuxin Cao, Kunming Liu, Jin-Biao Liu
Summary: We reported a reaction-based composite fluorescent probe for the detection and quantification of hydrogen peroxide in water samples.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Spectroscopy
Linlin Yang, Nan Yang, Pengli Gu, Chuang Wang, Beining Li, Yihua Zhang, Liguo Ji, Guangjie He
Summary: In this study, a simple ratiometric fluorescent probe based on a flavone skeleton was designed for highly selective detection of H2Sn. The probe exhibited a significant red-shift fluorescence emission in the presence of H2Sn, showing good linear relationship and low detection limit. It demonstrated high specificity and anti-interference ability for other related sulfur species, and showed promising application in the confocal imaging of H(2)S(n)in Hela cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
Article
Biochemistry & Molecular Biology
Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biophysics
Hiroshi Aoki, Masaki Torimura, Tetsuya Nakazato
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biophysics
Hiroshi Aoki, Robert M. Corn, Brandon Matthews
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Instruments & Instrumentation
Hiroshi Aoki, Takeshi Sukegawa, Masaki Torimura, Tetsuya Nakazato
SENSORS AND MATERIALS
(2020)
Article
Instruments & Instrumentation
Hiroshi Aoki, Tetsuya Nakazato
SENSORS AND MATERIALS
(2020)
Article
Instruments & Instrumentation
Yasumasa Kanekiyo, You Mitani, Miho Suda, Hiroshi Aoki, Hironobu Minai
SENSORS AND MATERIALS
(2020)
Article
Pharmacology & Pharmacy
Hiroshi Aoki, Hidenori Tani, Kaoru Nakamura, Hiroaki Sato, Masaki Torimura, Tetsuya Nakazato
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2020)
Correction
Chemistry, Physical
Haoyi Wu, Xiao-Li Wu, Zheng-Ming Wang, Hiroshi Aoki, Shuzo Kutsuna, Keiko Jimura, Shigenobu Hayashi
APPLIED CATALYSIS B-ENVIRONMENTAL
(2020)
Article
Chemistry, Analytical
Hiroshi Aoki, Masaki Torimura, Hiroshi Habe
Summary: This study explores a method to discriminate microplastics by analyzing the change in fluorescent properties after adsorption of fluorescent dyes onto microplastic surfaces. Fluorescein and Methylene Blue showed increased fluorescence intensity, while Rhodamine 6G showed negligible changes. This research demonstrates the potential to identify and estimate individual plastic materials by investigating appropriate fluorescent dyes.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Hiroshi Aoki
Summary: A novel technique for microscopic observation of single microplastic particles stained with fluorescent dyes has been developed, enabling fluorescence-based determination of the materials of these particles as a first screening of material determination. Commercially available microplastic particles and fluorescent dyes were used to observe the degree of fluorescent intensity for various combinations, revealing statistically significant differences in fluorescent intensity for different materials.
Article
Pharmacology & Pharmacy
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Masaki Torimura, Hiroaki Sato
DRUG DISCOVERIES AND THERAPEUTICS
(2017)
Article
Chemistry, Multidisciplinary
Xinping Liu, Zhengqing Yan, Yuhuan Sun, Jinsong Ren, Xiaogang Qu
CHEMICAL COMMUNICATIONS
(2017)
Article
Engineering, Biomedical
Hirokazu Nosato, Hidenori Sakanashi, Eiichi Takahashi, Masahiro Murakawa, Hiroshi Aoki, Ken Takeuchi, Yasuo Suzuki
INTERNATIONAL JOURNAL OF BIOMEDICAL IMAGING
(2017)
