Article
Biochemical Research Methods
Manuela Grimaldi, Carmen Marino, Michela Buonocore, Angelo Santoro, Eduardo Sommella, Fabrizio Merciai, Emanuela Salviati, Arianna De Rosa, Tommaso Nuzzo, Francesco Errico, Pietro Campiglia, Alessandro Usiello, Anna Maria D'Ursi
Summary: Originally believed to be present only in bacteria and invertebrates, D-amino acids have also been found in mammalian tissues. Depletion of D-Asp in the brain has been shown to influence amino acid pathways and metabolites related to brain development and function.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biology
Jason T. Lambert, Linda Su-Feher, Karol Cichewicz, Tracy L. Warren, Iva Zdilar, Yurong Wang, Kenneth J. Lim, Jessica L. Haigh, Sarah J. Morse, Cesar P. Canales, Tyler W. Stradleigh, Erika Castillo Palacios, Viktoria Haghani, Spencer D. Moss, Hannah Parolini, Diana Quintero, Diwash Shrestha, Daniel Vogt, Leah C. Byrne, Alex S. Nord
Summary: Enhancers are crucial regulatory elements in modulating gene expression for specific cell types and environmental conditions. High-throughput screening of candidate DNA sequences for enhancer activity has been made possible with the development of massively parallel reporter assays (MPRAs). Tissue-specific screening of enhancer function at scale has the potential to enhance our understanding of the roles of non-coding sequences in development, evolution, and disease.
Review
Neurosciences
Daria Andreeva, Lada Murashova, Nikita Burzak, Vyacheslav Dyachuk
Summary: Satellite glial cells (SGCs) surrounding neurons in ganglia are altered after nerve injury, affecting pain development and maintenance. SGCs in different ganglia exhibit heterogeneity and changes. Further research is needed to understand their role in chronic pain and other diseases.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Lecoq Lucie, Ernoult Aude, Fabre Elodie, Rapinel Sebastien, Carbonne Benjamin, Benoit Maube, Mony Cendrine
Summary: Agricultural intensification has caused the destruction of semi-natural habitats and simplified landscapes in North-Western Europe, leading to negative effects on plant assemblages and ecosystem functions. The relationship between biodiversity and productivity at the landscape scale is still not well understood in real-world systems. Conservation strategies, such as maintaining hedgerows, can help preserve plant functional diversity and ensure future fodder production.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2023)
Article
Neurosciences
Yanhao Chu, Shilin Jia, Ke Xu, Qing Liu, Lijia Mai, Jiawei Liu, Wenguo Fan, Fang Huang
Summary: This study reveals the transcript characteristics and heterogeneity of satellite glial cells (SGCs) in trigeminal ganglion (TG) and investigates the interactions between SGCs and neurons. The results show that TG consists of various cell clusters including neurons, SGCs, myeloid Schwann cells, non-myeloid Schwann cells, and immune cells, with corresponding markers identified. The findings provide valuable insights into studying SGCs in the TG.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Oncology
Rama Soundararajan, Paul Viscuse, Patrick Pilie, Jingjing Liu, Souzana Logotheti, Caddie Laberiano Fernandez, Daniele Lorenzini, Anh Hoang, Wei Lu, Luisa Maren Solis Soto, Ignacio I. Wistuba, Mingchu Xu, Xingzhi Song, Peter D. A. Shepherd, Nora M. Navone, Rebecca S. S. Tidwell, Guillermina Lozano, Christopher Logothetis, Jianhua Zhang, James P. Long, Marcos R. Estecio, Vasiliki Tzelepi, Ana M. Aparicio
Summary: Prostate cancer behaves differently in different patients, and understanding the molecular profiles of aggressive variants can inform targeted treatments. Alterations in the tumor suppressors TP53, RB1, and PTEN characterize the more virulent prostate cancers and predict their response to combination chemotherapies. Staining tumor tissues or examining their DNA can determine these molecular profiles.
Article
Agriculture, Dairy & Animal Science
Shu Wang, Haizhen Wang, Juan Liu, Xiaona Zhang, Yang Yang, Chang Lu, Chunbo Cai, Yan Zhao, Guoming Liang, Xiaohong Guo, Bugao Li, Guoqing Cao, Pengfei Gao
Summary: The study investigated the role of lnc-34015 in muscle development and immune regulation, revealing its associations with microRNAs and specific genes. Bioinformatics analysis showed that lnc-34015 is involved in important biological processes such as cell proliferation and stress response. The findings suggest that lnc-34015 plays a significant role in regulating porcine inflammatory responses.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hendrik J. Kuiken, Sabin Dhakal, Laura M. Selfors, Chandler M. Friend, Tian Zhang, Maurizio Callari, Ron C. J. Schackmann, G. Kenneth Gray, Jett Crowdis, Hyo-Eun C. Bhang, Timour Baslan, Frank Stegmeier, Steven P. Gygi, Carlos Caldas, Joan S. Brugge
Summary: Intratumoral heterogeneity exists in different tumor types and models, affecting tumor progression and drug resistance. In this study, subclonal populations derived from a triple-negative breast cancer cell line showed unique genetic alterations and varied in growth and tumor forming ability. Clonal dynamics analysis revealed selection for distinct populations in different environments, with one subclone showing selective advantage and decreased sensitivity to interferon signaling in xenograft tumors, suggesting potential implications for cancer and xenograft models.
Article
Pediatrics
Serdar Beken, Saygin Abali, Neslihan Yildirim Saral, Bengisu Guner, Taha Dinc, Eda Albayrak, Melike Ersoy, Meltem Kilercik, Muge Halici, Ezgi Bulbul, Didem Kaya, Melis Karabay, Zeynep Alize Ay, Gulten Zeynep Eksi, Fehime Benli Aksungar, Ayse Korkmaz, Mustafa Serteser
Summary: This study revealed differences in metabolomics profile in newborn infants with intrauterine growth problems in the early postnatal period, possibly reflecting distinct energy metabolism. These differences may lead to metabolic disturbances in future adulthood.
FRONTIERS IN PEDIATRICS
(2021)
Article
Agriculture, Dairy & Animal Science
Jinbi Zhang, Xinxin Qin, Caixia Wang, Yuge Zhang, Yizhe Dou, Shiyong Xu, Jingge Liu, Zengxiang Pan
Summary: This study used high-throughput RNA sequencing to identify differentially expressed genes in granulosa cells from healthy and early atretic ovarian follicles in pigs. GO analysis highlighted hormone metabolism, plasma membrane localization, and transporter activity, while pathway analysis revealed the involvement of steroidogenesis, cell adhesion molecules, and TGF-beta signaling pathways in atresia.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Elizabeth A. Rondini, Vanesa D. Ramseyer, Rayanne B. Burl, Roger Pique-Regi, James G. Granneman
Summary: The study revealed 3 major ASC subtypes in subcutaneous white adipose tissue (iWAT) in mice during perinatal and adult periods, with dynamic changes in transcriptomes and enhancer landscapes. Additionally, differences in gene expression were associated with developmental changes in chromatin accessibility and predicted transcription factor motifs in both stromal cells and adipocytes.
MOLECULAR METABOLISM
(2021)
Article
Genetics & Heredity
Zhuang Chai, Jing Wu, Zicheng Qi, Yan Liu, Yanjiao Lv, Yuting Zhang, Zhuoran Yu, Chaoqian Jiang, Zhonghua Liu
Summary: This study analyzed the gene structure and sequence characteristics of porcine NANOG, and investigated its expression patterns in early embryos. The porcine NANOG gene was found to be located on chromosome 5 and exhibited two transcripts, playing a crucial role in the development of early-stage embryos.
Article
Pediatrics
Marta Diaz, Alberto Mestres-Arenas, Carles Lerin, Ruben Cereijo, Abel Lopez-Bermejo, Francis de Zegher, Francesc Villarroya, Lourdes Ibanez
Summary: This study longitudinally assessed the impact of feeding patterns on PGRN concentrations in infants born with appropriate or small weight for gestational age. The results showed that PGRN levels were low at birth and increased at 4 and 12 months, with a smaller increase in small for gestational age infants. PGRN was associated with markers of obesity, inflammation, and insulin resistance, especially in formula-fed small for gestational age infants.
PEDIATRIC RESEARCH
(2023)
Article
Immunology
Jiamin Huang, Zhechen Zhu, Dongdong Ji, Ran Sun, Yunxi Yang, Lu Liu, Yiming Shao, Yi Chen, Linbin Li, Binwei Sun
Summary: This study analyzed circulating neutrophils (PMNs) in healthy donors and early burned patients using single-cell RNA sequencing, revealing the heterogeneity and functional multiplicity of PMNs in early stages of severe burn injuries. PMN activation, degranulation, chemotaxis, phagocytosis, and reactive oxygen species production significantly differed between different days in burned patients. Previously undescribed transcription factors were also identified.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Ellis Michiels, Hediel Madhloum, Silke Van Lint, Nouredin Messaoudi, Rastislav Kunda, Sandrina Martens, Philippe Giron, Catharina Olsen, Pierre Lefesvre, Nelson Dusetti, Leila EL Mohajer, Richard Tomasini, Lukas J. A. C. Hawinkels, Farah Ahsayni, Remy Nicolle, Tatjana Arsenijevic, Christelle Bouchart, Jean-Luc Van Laethem, Ilse Rooman
Summary: This study identifies two subtypes of pancreatic cancer and reveals spatial phenotypes within tumors through analysis of mRNA panels. These findings are important for understanding tumor heterogeneity and guiding therapeutic approaches.
JOURNAL OF PATHOLOGY
(2023)
Article
Genetics & Heredity
Katja Stange, Claus-Eric Ott, Mareen Schmidt-von Kegler, Gabriele Gillesen-Kaesbach, Stefan Mundlos, Katarina Dathe, Petra Seemann
JOURNAL OF HUMAN GENETICS
(2015)
Article
Genetics & Heredity
Katja Stange, Julie Desir, Naseebullah Kakar, Thomas D. Mueller, Birgit S. Budde, Christopher T. Gordon, Denise Horn, Petra Seemann, Guntram Borck
ORPHANET JOURNAL OF RARE DISEASES
(2015)
Article
Cell Biology
Claudia Miersch, Katja Stange, Monika Roentgen
Article
Cell Biology
Claudia Miersch, Katja Stange, Monika Roentgen
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2018)
Article
Biochemistry & Molecular Biology
Julia Haupt, Alexandra Deichsel, Katja Stange, Cindy Ast, Renata Bocciardi, Roberto Ravazzolo, Maja Di Rocco, Paola Ferrari, Antonio Landi, Frederick S. Kaplan, Eileen M. Shore, Carsten Reissner, Petra Seemann
HUMAN MOLECULAR GENETICS
(2014)
Article
Biochemistry & Molecular Biology
Katja Stange, Tino Thieme, Karen Hertel, Silke Kuhfahl, Andreas R. Janecke, Hildegunde Piza-Katzer, Maila Penttinen, Marja Hietala, Katarina Dathe, Stefan Mundlos, Elisabeth Schwarz, Petra Seemann
JOURNAL OF MOLECULAR BIOLOGY
(2014)
Article
Anatomy & Morphology
Laura Hildebrand, Mareen Schmidt-von Kegler, Maria Walther, Petra Seemann, Katja Stange
DEVELOPMENTAL DYNAMICS
(2019)
Article
Multidisciplinary Sciences
K. Stange, C. Miersch, G. Sponder, M. Roentgen
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Katja Stange, Hellen Elisa Ahrens, Julia von Maltzahn, Monika Roentgen
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2020)
Article
Multidisciplinary Sciences
Bianka Grunow, Katja Stange, Ralf Bochert, Katrin Toenissen
Summary: The growth and muscle metabolism of rainbow trout and maraena whitefish at slaughter size were examined, revealing significant interspecies differences, such as smaller diameter cells and higher stem cell proportion in red muscle of rainbow trout. These differences suggest a higher degree of hypertrophic growth in rainbow trout compared to maraena whitefish.
Article
Multidisciplinary Sciences
Yaolu Zhao, Elke Albrecht, Katja Stange, Zeyang Li, Johannes Schregel, Quentin L. Sciascia, Cornelia C. Metges, Steffen Maak
Summary: This study found that glutamine supplementation can improve muscle growth in low birth weight piglets by stimulating cell proliferation both in muscle tissue and in vitro myogenic cell culture, with minimal impact on the expression of muscle growth regulatory genes.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Katja Stange, Amir Keric, Andreas Friese, Monika Roentgen
Summary: This study used a mid-scale incubator and bioreactor system to produce spheroids from primary porcine muscle cells and C2C12 cells, and compared their characteristics. The results showed that both cell types formed spheroids after 24 hours, but C2C12 cells had larger spheroid diameter and lower number of aggregates. After dissociation and differentiation, both cell types were able to proliferate and differentiate into the myogenic lineage. This suggests that the mid-scale incubator and bioreactor system is suitable for spheroid cultivation of primary muscle cells.
Article
Biochemistry & Molecular Biology
Katja Stange, Toni Schumacher, Claudia Miersch, Rose Whelan, Martina Kluenemann, Monika Roentgen
Summary: Optimal supply of L-methionine promotes muscle growth, whereas over-supplementation has adverse effects. This study examines the effects of different methionine supplements on cell growth, viability, ROS production, and mitochondrial bioenergetics in mouse and quail myoblasts. Results show that all supplements stimulate cell growth, but high levels of L-methionine or DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA) retard growth and increase extracellular H2O2 levels, indicating oxidative stress. DL-HMTBA oversupplementation also leads to adaptive changes in mitochondrial functionality. Quail cells are better able to cope with methionine oversupplementation than mouse cells.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Laura Hildebrand, Katja Stange, Alexandra Deichsel, Manfred Gossen, Petra Seemann
CELLULAR SIGNALLING
(2017)