Article
Immunology
Emily Fraser, Laura Denney, Agne Antanaviciute, Karl Blirando, Chaitanya Vuppusetty, Yuejuan Zheng, Emmanouela Repapi, Valentina Iotchkova, Stephen Taylor, Neil Ashley, Victoria St Noble, Rachel Benamore, Rachel Hoyles, Colin Clelland, Joseph M. D. Rastrick, Clare S. Hardman, Nasullah K. Alham, Rachel E. Rigby, Alison Simmons, Jan Rehwinkel, Ling-Pei Ho
Summary: Monocytes in idiopathic pulmonary fibrosis (IPF) patients exhibit a distinct immunophenotype from age-matched controls, with an activated type I interferon pathway that may contribute to driving chronic inflammation and fibrosis. These findings strengthen the potential role of monocytes in the pathogenesis of IPF.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Carole Y. Perrot, Theodoros Karampitsakos, Jose D. Herazo-Maya
Summary: Pulmonary fibrosis is a disease caused by a variety of abnormal events. In idiopathic pulmonary fibrosis (IPF), inhalational, environmental, or occupational exposures in genetically and epigenetically predisposed individuals trigger a series of recurrent abnormal events, leading to alveolar epithelial cell injury, activation of coagulation pathways, and differentiation of monocytes into monocyte-derived alveolar macrophages (Mo-AMs). Immune dysregulation plays an important role in the onset and progression of IPF, and understanding the role of monocytes and Mo-AMs is crucial for potential targeted therapies.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Swee Heng Milon Pang, Joshua D'Rozario, Senora Mendonca, Tejasvini Bhuvan, Natalie L. Payne, Di Zheng, Assifa Hisana, Georgia Wallis, Adele Barugahare, David Powell, Jai Rautela, Nicholas D. Huntington, Grant Dewson, David C. S. Huang, Daniel H. D. Gray, Tracy S. P. Heng
Summary: Multipotent mesenchymal stromal cells (MSCs) have therapeutic benefits in preclinical models, with their apoptosis and immunosuppressive effects playing a crucial role in reducing disease severity and highlighting implications for cell-based therapies.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Karin Heidbreder, Katrin Sommer, Maximilian Wiendl, Tanja M. Mueller, Imke Atreya, Kai Hildner, Markus F. Neurath, Sebastian Zundler
Summary: This study investigates the role of non-classical monocytes in large intestinal wound healing. By depleting non-classical monocytes in mice deficient for the transcription factor Nr4a1, the researchers found that wound healing was significantly delayed and there was a reduced presence of macrophages with a wound healing phenotype. The findings suggest that non-classical monocytes are biased towards wound healing macrophages, providing insights into the modulation of intestinal macrophage functions through monocyte recruitment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Steven Droho, Amrita Rajesh, Carla M. Cuda, Harris Perlman, Jeremy A. Lavine
Summary: By performing single-cell RNA-Seq on immune cells from WT and Ccr2-/- mice, we identified macrophage heterogeneity in patients with nAMD. Spp1+ macrophages expressed a proangiogenic transcriptome through multiple pathways and CD11c+ macrophages were increased and present in CNV lesions. Depletion of CD11c+ macrophages reduced CNV size, suggesting their potential as therapeutic targets for treatment-resistant patients with nAMD.
Article
Immunology
Siddharth Krishnan, Kelly Wemyss, Ian E. Prise, Flora A. McClure, Conor O'Boyle, Hayley M. Bridgeman, Tovah N. Shaw, John R. Grainger, Joanne E. Konkel
Summary: HSPCs were found to reside and differentiate in healthy gingiva, a key oral barrier. Gingival HSPCs exhibited similar characteristics to bone marrow HSPCs but responded differently to inflammatory insults, only responding to oral rather than systemic inflammation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Critical Care Medicine
Steven L. Brody, Sean P. Gunsten, Hannah P. Luehmann, Debbie H. Sultan, Michelle Hoelscher, Gyu Seong Heo, Jiehong Pan, Jeffrey R. Koenitzer, Ethan C. Lee, Tao Huang, Cedric Mpoy, Shuchi Guo, Richard Laforest, Amber Salter, Tonya D. Russell, Adrian Shifren, Christophe Combadiere, Kory J. Lavine, Daniel Kreisel, Benjamin D. Humphreys, Buck E. Rogers, David S. Gierada, Derek E. Byers, Robert J. Gropler, Delphine L. Chen, Jeffrey J. Atkinson, Yongjian Liu
Summary: The Cu-64-DOTA-ECL1i PET uptake correlated with CCR2(+) cell infiltration in the lung associated with fibrosis, with inhibition of fibrosis by IL1 beta blockade or pirfenidone reducing CCR2(+) macrophage accumulation. CCR2(+) cells concentrated in perifibrotic regions in lung tissues from patients with IPF, exhibiting intensive signals in fibrotic zones compared to healthy volunteers. These findings suggest a role for imaging CCR2(+) cells within the fibrogenic niche in IPF for personalized therapy and monitoring.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Anna Alwani, Aneta Andreasik, Rafal Szatanek, Maciej Siedlar, Monika Baj-Krzyworzeka
Summary: Monocytes, as a type of blood cells, serve as a link between innate and adaptive immunity, being both precursors of macrophages and effector cells like phagocytes or cytotoxic cells. At the tumor site, monocytes/macrophages are important indicators for cancer recovery. The role of small regulatory miRNA in monocyte formation, maturation, and function is crucial.
Article
Cell Biology
Anna B. Montgomery, Shang Yang Chen, Yidan Wang, Gaurav Gadhvi, Maximilian G. Mayr, Carla M. Cuda, Salina Dominguez, Hadijat-Kubura Moradeke Makinde, Miranda G. Gurra, Alexander V. Misharin, Arthur M. Mandelin, Eric M. Ruderman, Anjali Thakrar, Simran Brar, Mary Carns, Kathleen Aren, Mahzad Akbarpour, Andrew Filer, Saba Nayar, Ana Teososio, Triin Major, Ankit Bharat, G. R. Scott Budinger, Deborah R. Winter, Harris Perlman
Summary: This study identifies and characterizes distinct populations of monocytes in RA, including a synovial monocyte population resembling circulating non-classical monocytes and a tissue-resident monocyte-lineage cell population. These tissue-resident monocytes are long lived and embryonically derived, independent of NR4A1 and CCR2, and crucial for the development of RA-like disease. They undergo increased proliferation and reverse diapedesis in response to arthrogenic stimuli, and their activation pathways overlap with those downregulated in LFA1-/- TR-MCs.
Article
Engineering, Biomedical
Bita Nasiri, Tai Yi, Yulun Wu, Randall J. Smith, Ashis Kumar Podder, Christopher K. Breuer, Stelios T. Andreadis
Summary: A strategy to recruit monocytes (MCs) from blood to regenerate vascular tissue from unseeded tissue engineered vascular grafts is presented. The fusion protein, H2R5, can capture blood-derived MC under static or flow conditions on the surface of vascular grafts. This bound MC then transforms into macrophages (M phi) expressing specific genes. When these functionalized grafts are implanted, they form a continuous endothelium expressing specific proteins and multiple cell layers expressing specific markers. Lineage tracing analysis provides direct evidence of MC/M phi recruitment to the graft lumen. Circulating MCs may potentially contribute to the endothelialization and vascular wall formation of acellular vascular grafts.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Neurosciences
Leena Ali Ibrahim, Shuhan Huang, Marian Fernandez-Otero, Mia Sherer, Yanjie Qiu, Spurti Vemuri, Qing Xu, Robert Machold, Gabrielle Pouchelon, Bernardo Rudy, Gord Fishell
Summary: The study reveals that the establishment of top-down connectivity in the primary visual cortex critically depends on early thalamic inputs during postnatal development. Thalamic inputs predominantly target neurogliaform cells in layer 1, facilitating the subsequent strengthening of top-down inputs from the anterior cingulate cortex onto these cells. Sensory deprivation or removal of thalamic afferents blocks this phenomenon, highlighting the importance of bottom-up inputs for the development of top-down connections in the visual cortex.
Article
Reproductive Biology
Luba Sominsky, Simin Younesi, Simone N. De Luca, Sophie M. Loone, Kylie M. Quinn, Sarah J. Spencer
Summary: The study reveals that ovarian follicles are resilient against short-term disturbances in circulating and ovarian monocytes, making them able to withstand acute changes in immune balance.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Di Cui, Alexandra Franz, Sophie A. Fillon, Linda Jannetti, Timo Isambert, Katrin Fundel-Clemens, Heinrich J. Huber, Coralie Viollet, Alexander Ghanem, Akira Niwa, Bernd Weigle, Stefan Pflanz
Summary: This study presents a fully optimized differentiation protocol of hiPSC-derived myeloid lineage cells and highlights their applications in research, drug screening, and cell therapies in functional verification.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Fang Niu, Ke Liao, Guoku Hu, Shamsudheen Moidunny, Sabita Roy, Shilpa Buch
Summary: This study demonstrates for the first time that exposure of human monocytes to HIV Tat protein upregulates CXCR3 expression, leading to increased monocyte transmigration across the blood-brain barrier. This process involves activation of TLR4, TBK1 phosphorylation, IRF3 nuclear translocation, ultimately enhancing CXCR3 expression in human monocytes. These findings suggest a novel molecular mechanism underlying HIV Tat-mediated increase in monocyte transmigration across the BBB, implicating a new role of CXCR3-dependent monocyte transmigration in HIV Tat-mediated neuroinflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xiaoqian Wei, Shushan Pan, Zirui Wang, Jieru Chen, Li Lu, Qizhi Cao, Shuling Song, Huachang Zhang, Xiaohui Liu, Xianjun Qu, Xiukun Lin, Huanli Xu
Summary: This study uncovered the critical role of LAIR1 in facilitating the malignant progression of glioma and demonstrated its support of an immunosuppressive glioma microenvironment through CCL5-mediated cell polarization.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Molly H. Jenkins, Walburga Croteauz, David W. Mullins, Constance E. Brinckerhoff
Article
Gastroenterology & Hepatology
Brian Bressler, Kevin P. Bethel, Ralf Kleef, Sophie L. Reynolds, Simon Sutcliffe, DavidW. Mullins, Hal Gunn
GASTROENTEROLOGY RESEARCH AND PRACTICE
(2015)
Article
Oncology
Eleanor Clancy-Thompson, Thomas J. Perekslis, Walburga Croteau, Matthew P. Alexander, Tamer B. Chabanet, Mary Jo Turk, Yina H. Huang, David W. Mullins
CANCER IMMUNOLOGY RESEARCH
(2015)
Article
Oncology
Ileana S. Mauldin, Nolan A. Wages, Anne M. Stowman, Ena Wang, Mark E. Smolkin, Walter C. Olson, Donna H. Deacon, Kelly T. Smith, Nadedja V. Galeassi, Kimberly A. Chianese-Bullock, Lynn T. Dengel, Francesco M. Marincola, Gina R. Petroni, David W. Mullins, Craig L. Slingluff
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2016)
Article
Oncology
Ileana S. Mauldin, Nolan A. Wages, Anne M. Stowman, Ena Wang, Walter C. Olson, Donna H. Deacon, Kelly T. Smith, Nadedja Galeassi, Jessica E. Teague, Mark E. Smolkin, Kimberly A. Chianese-Bullock, Rachael A. Clark, Gina R. Petroni, Francesco M. Marincola, David W. Mullins, Craig L. Slingluff
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2016)
Review
Immunology
Frederick S. Varn, David W. Mullins, Hugo Arias-Pulido, Steven Fiering, Chao Cheng
Article
Oncology
Chery A. Whipple, Andrea Boni, Jan L. Fisher, Thomas H. Hampton, Gregory J. Tsongalis, Diane L. Mellinger, Shaofeng Yan, Laura J. Tafe, Constance E. Brinckerhoff, Mary J. Turk, David W. Mullins, Camilo E. Fadul, Marc S. Ernstoff
Article
Multidisciplinary Sciences
Frederick S. Varn, Erik H. Andrews, David W. Mullins, Chao Cheng
NATURE COMMUNICATIONS
(2016)
Article
Oncology
Mark Bazett, Amanda M. Costa, Momir Bosiljcic, Rebecca M. Anderson, Matthew P. Alexander, Stephanie W. Y. Wong, Salim Dhanji, Jenny M. H. Chen, Jim Pankovich, Stephen Lam, Simon Sutcliffe, Hal Gunn, Shirin Kalyan, David W. Mullins
Article
Oncology
Matthew P. Alexander, Steven N. Fiering, Gary R. Ostroff, Robert A. Cramer, David W. Mullins
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2018)
Article
Oncology
Molly H. Jenkins, Shannon M. Steinberg, Matthew P. Alexander, Jan L. Fisher, Marc S. Ernstoff, Mary Jo Turk, David W. Mullins, Constance E. Brinckerhoff
PIGMENT CELL & MELANOMA RESEARCH
(2014)
Article
Multidisciplinary Sciences
Molly H. Jenkins, Constance E. Brinckerhoff, David W. Mullins
Article
Multidisciplinary Sciences
Shirin Kalyan, Mark Bazett, Ho Pan Sham, Momir Bosiljcic, Beryl Luk, Salim Dhanji, Amanda M. Costa, Stephanie W. Y. Wong, Mihai G. Netea, David W. Mullins, Hal Gunn
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Frederick S. Varn, Yue Wang, David W. Mullins, Steven Fiering, Chao Cheng