Article
Agriculture, Multidisciplinary
Xingtao Zhou, Duoduo Zhang, Wucheng Qi, Tao Hong, Tao Xiong, Tingqin Wu, Fang Geng, Mingyong Xie, Shaoping Nie
Summary: EPS116 promotes intestinal epithelial regeneration by increasing the number of intestinal stem cells and modulating the gut microbiota profile, thereby maintaining intestinal barrier and homeostasis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Yunyun Jiang, Min Zhang, Yang Zhang, Justyna Zulewska, Zhennai Yang
Summary: This study investigated the impact of Ca2+ on EPS biosynthesis in the probiotic Lactobacillus plantarum K25. Results showed that Ca2+ increased EPS yield, changed monosaccharide composition, upregulated proteins related to EPS synthesis, and influenced small molecule metabolites related to EPS biosynthesis pathways. The activation of specific signaling pathways by Ca2+ provided insights into the regulation of EPS synthesis genes in L. plantarum K25.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Shilei Huang, Qiuhan Yu, Luyi Xie, Ling Ran, Kai Wang, Yang Yang, Lu Gan, Zhenhui Song
Summary: The study showed that Lactobacillus plantarum metabolites and exopolysaccharides have inhibitory effects on Porcine Epidemic Diarrhea Virus replication, with exopolysaccharides exhibiting strong antiviral properties. Exopolysaccharides can prevent virus adsorption and induce early apoptosis of infected cells.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Jing-Ting Chiou, Liang-Jun Wang, Yuan-Chin Lee, Long-Sen Chang
Summary: This study investigated the mechanism of Naja atra CTX1-induced cell death in leukemia cell lines. Results showed that CTX1 increased Ca2+ and ROS levels, leading to cell death through activation of the NOX4/ROS/p38 MAPK axis and subsequent activation of c-Jun-mediated Fas and ATF-2-mediated FasL transcription. The study also found that only CTX1 induced Fas/FasL expression and the intact Met residues were essential for its activity.
Article
Biochemistry & Molecular Biology
Jia-Jia Wen, Ming-Zhi Li, He Gao, Jie-Lun Hu, Qi-Xing Nie, Hai-Hong Chen, Yan-Li Zhang, Ming-Yong Xie, Shao-Ping Nie
Summary: This study demonstrated that polysaccharides from fermented and non-fermented Momordica charantia L. with Lactobacillus plantarum NCU116 (FP and NFP) can improve serum metabolism and gut microbiota in obese rats, leading to weight loss and reduction in serum lipids through promoting beneficial bacteria growth and reducing harmful bacteria. These findings suggest that probiotic products containing such polysaccharides may be promising for preventing or treating obesity.
Article
Biotechnology & Applied Microbiology
Kun-tai Li, Shuai-ying Peng, Bao Zhang, Wei-fu Peng, Shui-jing Yu, Xin Cheng
Summary: Exopolysaccharides (EPSs) can alleviate the toxic effect of cadmium (Cd) on rice and reduce Cd accumulation in grains. EPSs can facilitate the accumulation of Cd in rice roots and reduce Cd transportation from roots to leaves. Additionally, EPSs can affect the subcellular distribution and chemical forms of Cd in different parts of rice seedlings.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
M. P. Soumya, Reeba Parameswaran, K. Madhavan Nampoothiri
Summary: Glycosyltransferases synthesize exopolysaccharides (EPS) by modifying the properties of the polymers, such as glycosidic linkage, branching, length, mass, and conformation. A study on Lactobacillus plantarum BR2 (Accession No: MN176402) identified twelve glycosyltransferase genes, including BR2gtf (1116 bp), which was cloned into the pNZ8148 vector as an EPS biosynthetic glycosyltransferase. The over-expression of gtf gene in L. plantarum BR2 using a nisin-controlled expression system resulted in a 54.4% increase in EPS production, reaching a maximum of 23.2 ± 0.5 g/L in a 5 L bioreactor study after 72 h of fermentation. This study demonstrates a potential molecular strategy to enhance exopolysaccharide production in lactic acid bacteria.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Fangfang Min, Jielun Hu, Yadong Zhong, Tao Huang, Yousheng Huang, Shaoping Nie, Tao Xiong, Mingyong Xie
Summary: A previous study found that L. plantarum NCU1125, derived from fermented Nanfeng mandarin, survived in vitro simulated gastrointestinal digestion, suggesting potential probiotic properties. The current study demonstrated that L. plantarum NCU1125 could repair the intestinal physical barrier, strengthen the immunologic barrier, and reshape the microbial barrier. It also activated the TLR4/MyD88/NF-kappa B pathway and controlled intestinal inflammation.
Article
Biotechnology & Applied Microbiology
M. P. Soumya, K. Madhavan Nampoothiri
Summary: This study focuses on the production and chemical modifications of EPS produced by Lactobacillus plantarum BR2, and the evaluation of their biological properties. The results show that the modified derivatives have better antioxidant properties compared to the unmodified form. Among the derivatives, acetylated EPS (a-EPS) exhibits the highest DPPH radical scavenging and total antioxidant activity, while sulphated EPS (s-EPS) shows the highest reducing power potential and hydroxyl radical scavenging activities. The improved antioxidant properties open up better avenues for EPS application in the food and pharma sectors.
Article
Cell Biology
Yong-Ping Jian, Ge Yang, Li-Hong Zhang, Ji-Yong Liang, Hong-Lan Zhou, Yi-Shu Wang, Zhi-Xiang Xu
Summary: Abdominal irradiation can disrupt the intestinal mucosal barrier and lead to severe intestinal infection and multiple organ dysfunction syndromes. The role of intestinal microbiota in radiation-induced intestinal injury is still largely unknown. However, studies have shown that alterations in the composition of the microbiota, particularly a reduction in Lactobacillus, play a critical role in the development of intestinal injury following irradiation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiahuan Dong, Lijun Ping, Kangyong Zhang, Hongwei Tang, Jie Liu, Deyu Liu, Li Zhao, Smith Etareri Evivie, Bailiang Li, Guicheng Huo
Summary: Mixed L. plantarum can enhance intestinal immunity, reduce LPS-induced intestinal injury, and improve the integrity of the intestinal mucosal barrier.
Article
Medicine, Research & Experimental
Zhibo Zeng, Zonghao Huang, Wen Yue, Shah Nawaz, Xinzhu Chen, Jing Liu
Summary: The administration of Lactobacillus plantarum has significant immunoenhancing effects in immunosuppressed mice, restoring specific immune markers in the spleen and increasing the number of goblet cells in the intestine. Microbial taxonomic analysis showed changes in the gut microbiota composition, with a decrease in Firmicutes and an increase in Verrucomicrobia and Actinobacteria. Additionally, treatment with Lactobacillus plantarum restored mRNA expression of inflammatory cytokines.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Yangyan Yin, Yuying Liao, Jun Li, Zhe Pei, Leping Wang, Yan Shi, Hongyan Peng, Yizhou Tan, Changting Li, Huili Bai, Chunxia Ma, Yu Gong, Tianchao Wei, Hao Peng
Summary: This study found that Lactobacillus plantarum GX17 can be used as a feed additive to improve the growth performance and intestinal barrier function of yellow-feather chickens. It has more positive effects compared to commercial complex probiotics and can benefit the Chinese poultry industry.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Keying An, Wenwen Gao, Peng Li, Lei Li, Zhaofei Xia
Summary: This study found that dietary Lactobacillus plantarum (LP) improved the growth performance and intestinal health of Pekin ducks, with the 400 mg/kg LP dosage showing better effects.
Article
Biochemistry & Molecular Biology
Renjie Shi, Jin Ye, Hua Fan, Chunxia Xiao, Danna Wang, Bing Xia, Zhenting Zhao, Beita Zhao, Xiaoshuang Dai, Xuebo Liu
Summary: Lactobacillus plantarum LLY-606 can affect the development of hyperuricemia by modulating gut microbiota, downregulating renal inflammation, and regulating uric acid metabolism.
Review
Food Science & Technology
Bing Zheng, Xingtao Zhou, Xiaobo Hu, Yi Chen, Jianhua Xie, Qiang Yu
Summary: Polysaccharides have various biological activities, making them a potential natural drug for treating diseases. Apoptosis and autophagy play important roles in human health, and the regulation of apoptosis and autophagy by polysaccharides varies in different disease models. This review summarizes the relationship between apoptosis/autophagy and common human diseases, and discusses the role of apoptosis/autophagy pathway in regulating human health by polysaccharides.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Xincheng Wu, Xiaojun Huang, Wanning Ma, Mingzhi Li, Jiajia Wen, Chunhua Chen, Liandi Liu, Shaoping Nie
Summary: This study compared the intestinal immunomodulatory mechanisms of nine representative bioactive polysaccharides. The results revealed that these polysaccharides promote intestinal immunity through different ways, such as alleviating immune suppression in intestinal mucosal T cells, improving the intestinal microenvironment, promoting the colonization of beneficial bacteria, inhibiting the colonization of harmful bacteria, and alleviating the accumulation of certain metabolites in the large intestine. These findings provide a guideline for the development of superior intestinal immunomodulatory polysaccharides.
Article
Biochemistry & Molecular Biology
Shuping Chen, Junqiao Wang, Nan Dong, Qiuyue Fang, Yanli Zhang, Chunhua Chen, Steve W. W. Cui, Shaoping Nie
Summary: Polysaccharides from Cordyceps sinensis showed promising effects on experimental acute ulcerative colitis in mice. They alleviated colitis symptoms, inhibited NF-kappa B pathway activation, and modulated the gut microbiota composition. These findings suggest that Cordyceps sinensis polysaccharides have potential as a treatment for ulcerative colitis.
Review
Chemistry, Applied
Yan Shi, Dun Si, Donghong Chen, Zhigang Han, Qiang Yu, Xinfeng Zhang, Jingjing Liu, Jinping Si
Summary: Species in the genus Polygonatum, traditional food and medicinal plants, have an important role in controlling blood glucose. In this review, we summarized the traditional and modern applications of Polygonatum in diabetes mellitus, focusing on the material bases of polysaccharides, flavonoids, and saponins. The mechanisms of action include preventing obese diabetes, improving insulin resistance, promoting insulin secretion, regulating intestinal microecology, inhibiting advanced glycation end products accumulation, suppressing carbohydrate digestion and absorption, and modulating gluconeogenesis. Based on the safety and efficacy of this 'medicinal food' in diabetes prevention and treatment, we proposed a research and development program that includes diet design, medical nutrition therapy, and new drugs, which could provide new pathways for the use of natural plants in diabetes prevention and treatment.
Article
Chemistry, Applied
Ruihong Dong, Jinlong Tian, Ziyan Huang, Qiang Yu, Jianhua Xie, Bin Li, Chang Li, Yi Chen
Summary: This study investigated the protective effect of beta-glucan (BG), konjac glucomannan (KGM), and xanthan gum (XG) on the thermo-stability and antioxidant capacities of blueberry anthocyanins (ACN) and their interactions. The results showed that these three polysaccharides (XG > KGM > BG) can preserve the color, stability, and antioxidant capabilities of ACN. The interaction between ACN and specific zones of these polysaccharides was confirmed through UV-visible spectra, SEM, and DLS. The introduction of the three polysaccharides, especially XG, improved the thermostability of ACN through intermolecular interactions mainly via hydrogen bonding.
Article
Chemistry, Applied
You Long, Yousheng Huang, Mengting Zhu, Yingjie Ma, Bei Gan, YuanXing Wang, Qiang Yu, Jianhua Xie, Yi Chen
Summary: A robust and sensitive UHPLC-MS/MS method was developed for the simultaneous determination of 9 mycotoxins, AA, and 5-HMF. EMR-lipid d-SPE was used for clean-up of the extracts, and a new column phase (C18-PFP) was selected for HPLC separation. The method showed good linear relations and high recovery rates, making it a potential tool for cookies quality control.
Article
Food Science & Technology
Hanyu Lu, Mingyue Shen, Yi Chen, Qiang Yu, Ting Chen, Jianhua Xie
Summary: This study explored the therapeutic effects and underlying mechanisms of Cyclocarya paliurus polysaccharide (CP) and Chinese yam polysaccharide (CY) on dextran sulfate sodium (DSS)-induced mice colitis model. CP and CYP improved colitis symptoms, enhanced IL-10 production, inhibited cytokines (IL-1 beta, TNF-alpha), and reduced MPO activity. They also maintained intestinal integrity, reduced LBP and ET levels, and modulated gut microbiota composition and metabolism. These findings suggest that polysaccharides may alleviate colitis development and have implications for further research.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Tongwen Huang, Ke Zhang, Junqiao Wang, Kaihong He, Xingtao Zhou, Shaoping Nie
Summary: This study found that quercetin can alleviate the hepatotoxicity caused by acrylamide generated in heat-treated foods. Experiments on mice showed that quercetin can reduce the oxidative stress levels induced by acrylamide and protect the liver from damage. The results demonstrated that quercetin suppressed acrylamide-induced ferroptosis by inhibiting oxidative stress-driven autophagy, leading to the mitigation of liver injury.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Jinjin Liu, Junyi Yin, Xiaojun Huang, Cencen Liu, Liuyun Hu, Yansheng Huang, Fang Geng, Shaoping Nie
Summary: This study aimed to remove the controversial anthraquinones from Cassia obtusifolia seed water extracts (CWEs) using baking treatment (BT), stir-frying treatment (ST), and adsorption treatment (AT). Results showed that AT had the best removal effect on total anthraquinones and increased the content of neutral sugars in CWEs. However, AT decreased the antioxidant activity of CWEs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Chunhua Chen, Yanli Zhang, Hongmei Yin, Jielun Hu, Yadong Zhong, Shaoping Nie, Mingyong Xie
Summary: This study comprehensively investigated and compared the fermentation characteristics of indigestible glucans (IGs) with different structural properties and their effects on the gut microbiota. The results showed that different IGs were utilized by the gut microbiota at varying rates, and some remained unfermented after 48 hours. During fermentation, IGs released mono-, di-, or trisaccharides and lowered the pH of the broth, while producing short-chain fatty acids. The analysis also revealed that different IGs selectively promoted the proliferation of specific bacteria in the gut.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Yu Wu, Wengan Zhou, RuTing Feng, Duoduo Zhang, Yongkang Yu, Zuanyuan Huang, Junyi Yin, Xingtao Zhou, Shaoping Nie
Summary: The immunomodulatory effects of glucan from fruit body of Dictyophora rubrovolvata (DRP) were evaluated in this study. It was found that DRP can recover CTX-induced immunosuppression and activate dendritic cells (DCs) to increase cytokine secretion. The study also revealed that DRP regulates the AKT-STAT1 signaling pathway via Mincle in DCs to induce CD4(+) T cell proliferation and differentiation, thereby ameliorating CTX-induced immunosuppression.
Article
Chemistry, Multidisciplinary
Anqi Xie, Haihua Ji, Zheyi Liu, Yiqun Wan, Xuecong Zhang, Huihuang Xiong, Shao-Ping Nie, Hao Wan
Summary: Oral administration of probiotics is a promising method to alleviate inflammatory bowel diseases (IBDs), but sensitivity to the gastrointestinal environment and poor intestinal colonization hinder their effectiveness. In this study, a modified prebiotic-based shield (Fe-TA@mGN) was developed to enhance the stress resistance ability and intestinal colonization of Escherichia coli Nissle 1917 (EcN). The shield improved the survival rate of EcN and its retention in the intestine, leading to efficient alleviation of colitis. Additionally, the shield synergized with EcN to regulate gut microbiota and promote the production of short-chain fatty acids, further enhancing the alleviation of colitis.
Article
Chemistry, Applied
Ziwei Wang, Kunying Chen, Ke Zhang, Kaihong He, Duoduo Zhang, Xiaohan Guo, Tongwen Huang, Jielun Hu, Xingtao Zhou, Shaoping Nie
Summary: ACP inhibits proliferation of colorectal cancer cells by inducing apoptosis and lysosomal dysfunction through regulation of mitochondria, lysosome, and apoptosis-related pathways. It also increases histone H3-lysine-27 acetylation (H3K27ac) on CTSD promoter, facilitating TFEB binding and CTSD expression. ACP has potential as an anti-cancer agent for colorectal cancer treatment.
CARBOHYDRATE POLYMERS
(2023)
Article
Food Science & Technology
Xiaoting Qin, Chaoran Yang, Jingyu Si, Yi Chen, Jianhua Xie, Jie Tang, Xuewen Dong, Yanan Cheng, Xiaobo Hu, Qiang Yu
Summary: Superfine grinding combined with L. paracasei fermentation was used to improve the quality of soluble dietary fibers (SDFs). The modified SDFs showed higher crystallinity, thermal stability, and adsorption capacity. Principal component analysis evaluated FSS-SDF as having superior functional properties. Applying FSS-SDF to yogurt enhanced its gel strength and odor characteristics.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Meeting Abstract
Oncology
Kelly E. Rentscher, Wanting Zhai, Brent J. Small, Jaeil Ahn, Tim A. Ahles, Traci N. Bethea, Elizabeth C. Breen, Harvey J. Cohen, Martine Extermann, Deena M. A. Graham, Paul B. Jacobsen, Heather S. L. Jim, Brenna C. McDonald, Zev M. Nakamura, Sunita K. Patel, James C. Root, Andrew J. Saykin, Danielle B. Tometich, Kathleen M. Van Dyk, Xingtao Zhou, Jeanne S. Mandelblatt, Judith E. Carroll