期刊
ARABIAN JOURNAL OF CHEMISTRY
卷 10, 期 2, 页码 215-225出版社
ELSEVIER
DOI: 10.1016/j.arabjc.2016.01.011
关键词
Copper oxide nanoparticles; Black beans; HeLa cells; In-vitro anticancer
资金
- KU Research Professor Program
- Konkuk University, Seoul, South Korea
- Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Education [2015R1A2A2A01003741]
- National Research Foundation of Korea [2015R1A2A2A01003741] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Copper oxide nanoparticles (CuO NPs) were synthesized by a green route using an aqueous black bean extract and characterized by XRD, FT-IR, XPS, Raman spectroscopy, DLS, TEM, SAED, SEM, and EDX. The synthesized CuO NPs were spherical in shape, and the XRD results show the average size of the NPs was similar to 26.6 nm. The cytotoxic effect of the CuO NPs was determined by sulforhodamine-B assay. Mitochondria-derived reactive oxygen species (ROS) were increased and initiated lipid peroxidation of the liposomal membrane, which regulates several signaling pathways and influences the cytokinetic movements of cells. Mitochondrial fragmentation disruption assay confirmed the alteration in the mitochondrial structure after incubation with nanoparticles. In addition, clonogenic assay confirmed the inability of NPs incubated cancer cells to proliferate well. Our experimental results show that the CuO NPs can induce apoptosis and suppress the proliferation of HeLa cells. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
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