Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability

Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells

(2017) Bochao Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stem cell divisions, somatic mutations, cancer etiology, and cancer prevention

(2017) Cristian Tomasetti et al.   SCIENCE

Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1

(2017) Fahad Rashid et al.   eLife

DNA and Protein Requirements for Substrate Conformational Changes Necessary for Human Flap Endonuclease-1-catalyzed Reaction

(2016) Sana I. Algasaier et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site

(2016) Jack C Exell et al.   Nature Chemical Biology

Direct observation of DNA threading in flap endonuclease complexes

(2016) Faizah A AlMalki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation

(2016) H Sun et al.   ONCOGENE

Capture of a third Mg2+ is essential for catalyzing DNA synthesis

(2016) Y. Gao et al.   SCIENCE

Direct observation of DNA threading in flap endonuclease complexes

(2016) Faizah A AlMalki et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A new paradigm of DNA synthesis: three-metal-ion catalysis

(2016) Wei Yang et al.   Cell and Bioscience

Okazaki fragment maturation involves  -segment error editing by the mammalian FEN1/MutS  functional complex

(2015) S. Liu et al.   EMBO JOURNAL

FANCD2-associated Nuclease 1, but Not Exonuclease 1 or Flap Endonuclease 1, Is Able to Unhook DNA Interstrand Cross-linksin Vitro

(2015) Julia Pizzolato et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

End of cancer-genome project prompts rethink

(2015) Heidi Ledford   NATURE

Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA

(2015) Yijin Liu et al.   Cell Reports

Flap Endonuclease Activity of Gene 6 Exonuclease of Bacteriophage T7

(2014) Hitoshi Mitsunobu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Visualizing phosphodiester-bond hydrolysis by an endonuclease

(2014) Rafael Molina et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding

(2014) Michał Miętus et al.   NUCLEIC ACIDS RESEARCH

Flap Endonuclease 1

(2013) Lata Balakrishnan et al.   Annual Review of Biochemistry

Observing a DNA Polymerase Choose Right from Wrong

(2013) Bret D. Freudenthal et al.   CELL

The balancing act of DNA repeat expansions

(2013) Jane C Kim et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Expression levels of DNA replication and repair genes predict regional somatic repeat instability in the brain but are not altered by polyglutamine disease protein expression or age

(2013) Amanda G. Mason et al.   HUMAN MOLECULAR GENETICS

Proline Scanning Mutagenesis Reveals a Role for the Flap Endonuclease-1 Helical Cap in Substrate Unpairing

(2013) Nikesh Patel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Observation of unpaired substrate DNA in the flap endonuclease-1 active site

(2013) L. David Finger et al.   NUCLEIC ACIDS RESEARCH

Sequential and Multistep Substrate Interrogation Provides the Scaffold for Specificity in Human Flap Endonuclease 1

(2013) Mohamed A. Sobhy et al.   Cell Reports

An Evolutionarily Conserved Synthetic Lethal Interaction Network Identifies FEN1 as a Broad-Spectrum Target for Anticancer Therapeutic Development

(2013) Derek M. van Pel et al.   PLoS Genetics

Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

(2012) Amanda Beddows et al.   CHEMICAL COMMUNICATIONS

Somatic instability of the expanded CTG triplet repeat in myotonic dystrophy type 1 is a heritable quantitative trait and modifier of disease severity

(2012) Fernando Morales et al.   HUMAN MOLECULAR GENETICS

Double strand binding–single strand incision mechanism for human flap endonuclease: Implications for the superfamily

(2012) Susan E. Tsutakawa et al.   MECHANISMS OF AGEING AND DEVELOPMENT

Genome-wide Screen Identifies Pathways that Govern GAA/TTC Repeat Fragility and Expansions in Dividing and Nondividing Yeast Cells

(2012) Yu Zhang et al.   MOLECULAR CELL

Watching DNA polymerase η make a phosphodiester bond

(2012) Teruya Nakamura et al.   NATURE

Flap endonucleases pass 5′-flaps through a flexible arch using a disorder-thread-order mechanism to confer specificity for free 5′-ends

(2012) Nikesh Patel et al.   NUCLEIC ACIDS RESEARCH

Role of DNA Polymerases in Repeat-Mediated Genome Instability

(2012) Kartik A. Shah et al.   Cell Reports

Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily

(2011) Susan E. Tsutakawa et al.   CELL

Structures of Human Exonuclease 1 DNA Complexes Suggest a Unified Mechanism for Nuclease Family

(2011) Jillian Orans et al.   CELL

Unpairing and gating: sequence-independent substrate recognition by FEN superfamily nucleases

(2011) Jane A. Grasby et al.   TRENDS IN BIOCHEMICAL SCIENCES

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Flap Endonuclease 1 Mechanism Analysis Indicates Flap Base Binding Prior to Threading

(2010) Jason W. Gloor et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The 3′-Flap Pocket of Human Flap Endonuclease 1 Is Critical for Substrate Binding and Catalysis

(2009) L. David Finger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Coordination between Polymerase β and FEN1 Can Modulate CAG Repeat Expansion

(2009) Yuan Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Large-Scale Expansions of Friedreich's Ataxia GAA Repeats in Yeast

(2009) Alexander A. Shishkin et al.   MOLECULAR CELL

Active site substitutions delineate distinct classes of eubacterial flap endonuclease

(2008) Lee M. Allen et al.   BIOCHEMICAL JOURNAL

Three Metal Ions Participate in the Reaction Catalyzed by T5 Flap Endonuclease

(2008) Karl Syson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY