Article
Multidisciplinary Sciences
Zhuoyi Liang, Vipul Kumar, Marie Le Bouteiller, Jeffrey Zurita, Josefin Kenrick, Sherry G. Lin, Jiangman Lou, Jianqiao Hu, Adam Yongxin Ye, Cristian Boboila, Frederick W. Alt, Richard L. Frock
Summary: The study demonstrates that in Lig4-deficient G1-arrested progenitor B cells, Ku-dependent blockage of A-EJ leads to functional endjoining suppression. However, joining of RAG-generated DSBs in Ku70-deficient or Ku70/Lig4 double-deficient cells occurs through a translocation-like A-EJ mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Haibo Yang, Emily M. Lachtara, Xiaojuan Ran, Jessica Hopkins, Parasvi S. Patel, Xueping Zhu, Yao Xiao, Laiyee Phoon, Boya Gao, Lee Zou, Michael S. Lawrence, Li Lan
Summary: This study reveals the crucial role of RNA m5C modification in R-loops in regulating DNA damage repair pathways, promoting TC-HR and suppressing Alt-NHEJ. Simultaneous disruption of TC-HR and Alt-NHEJ exhibits synergistic cytotoxic effects on cancer cells, suggesting a potential strategy for cancer therapy.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Xikui Sun, Jingning Bai, Jiejie Xu, Xiaoli Xi, Mingyu Gu, Chengming Zhu, Hongman Xue, Chun Chen, Junchao Dong
Summary: The study demonstrates the critical roles of CtIP and Mre11 in mediating A-EJ CSR, while DNA2 and BLM are involved in long S region DSB resection. Additionally, ATM and its kinase activity can function partly independently of CtIP/Mre11 to mediate A-EJ switching.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yijang Xu, Hang Zhou, Ginell Post, Hong Zan, Paolo Casali
Summary: The study demonstrates that Rad52 mediates IgD CSR through a microhomology-based DNA repair pathway, leading to the expression of IgD in B cells.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Jana Koers, Casper Marsman, Juulke Steuten, Simon Tol, Ninotska I. L. Derksen, Anja ten Brinke, S. Marieke van Ham, Theo Rispens
Summary: In this study, the researchers used optimized in vitro culture conditions and in-depth assessment of B cell characteristics and signaling pathways to investigate the role of oxygen in regulating human naive B cell differentiation and class switch recombination. They found that normoxia promotes differentiation into functional antibody secreting cells, while a unique population of CD27(++) B cells is generated under hypoxia. Additionally, time-dependent transitions between hypoxic and normoxic oxygen levels during culture can direct different trajectories of human B cell differentiation and IgG class switch recombination.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tamara Murmann-Konda, Aashish Soni, Martin Stuschke, George Iliakis
Summary: The study demonstrates that low doses of ionizing radiation primarily repair DSBs through HR, while high doses mainly utilize c-NHEJ. In G(2)-phase cells, DSBs causing chromatid breaks are also repaired in a similar fashion. Furthermore, a pathway switch from HR to c-NHEJ occurs with increasing radiation dose in the processing of these specific DSBs.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2021)
Article
Immunology
Clayton A. White, Egest J. Pone, Tonika Lam, Connie Tat, Ken L. Hayama, Guideng Li, Hong Zan, Paolo Casali
JOURNAL OF IMMUNOLOGY
(2014)
Article
Surgery
Trinidad Cisneros, Danielle W. Dillard, Xiumei Qu, Justin Arredondo-Guerrero, Martha Castro, Steven Schaffert, Renata Martin, Carlos O. Esquive, Sheri M. Krams, Olivia M. Martinez
AMERICAN JOURNAL OF TRANSPLANTATION
(2019)
Article
Immunology
Hui Yan, Maria Fernandez, Jingwei Wang, Shuai Wu, Rui Wang, Zheng Lou, Justin B. Moroney, Carlos E. Rivera, Julia R. Taylor, Huoqun Gan, Hong Zan, Dmytro Kolvaskyy, Dongfang Liu, Paolo Casali, Zhenming Xu
JOURNAL OF IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Helia N. Sanchez, Justin B. Moroney, Huoqun Gan, Tian Shen, John L. Im, Tianbao Li, Julia R. Taylor, Hong Zan, Paolo Casali
NATURE COMMUNICATIONS
(2020)
Article
Immunology
Paolo Casali, Tian Shen, Yijiang Xu, Zhifang Qiu, Daniel P. Chupp, John Im, Zhenming Xu, Hong Zan
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Huoqun Gan, Tian Shen, Daniel P. Chupp, Julia R. Taylor, Helia N. Sanchez, Xin Li, Zhenming Xu, Hong Zan, Paolo Casali
Article
Multidisciplinary Sciences
Justin B. Moroney, Anusha Vasudev, Alexander Pertsemlidis, Hong Zan, Paolo Casali
NATURE COMMUNICATIONS
(2020)