Article
Cardiac & Cardiovascular Systems
Kuo-Tzu Sung, Jen-Yuan Kuo, Chun-Ho Yun, Yueh-Hung Lin, Jui-Peng Tsai, Chi-In Lo, Chih-Chung Hsiao, Yau-Huei Lai, Cheng-Ting Tsai, Charles Jia-Yin Hou, Cheng-Huang Su, Hung- Yeh, Chen-Yen Chien, Ta-Chuan Hung, Chung-Lieh Hung
Summary: Region-specific visceral adiposity may not equally discriminate baseline dysglycemia or diabetes, and showed differential predictive performance in new-onset diabetes. Our data suggested that interatrial fat may serve as a potential marker for new-onset diabetes.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Review
Biochemistry & Molecular Biology
Jorge Valencia-Ortega, Renata Saucedo, Martha A. Sanchez-Rodriguez, Jose G. Cruz-Duran, Edgar G. Ramos Martinez
Summary: Gestational diabetes mellitus is the most common metabolic complication in pregnancy, involving various factors in its pathophysiology. Studies have observed that alterations in DNA methylation patterns and certain microRNA levels are related to the disease characteristics, highlighting the need for further research in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Kevin Roth, Louis Coussement, Elena V. Knatko, Maureen Higgins, Sandra Steyaert, Charlotte M. Proby, Tim de Meyer, Albena T. Dinkova-Kostova
Summary: The study found increased average methylation levels in mouse cSCC, especially in stem-cell like human cSCC. There were striking similarities in differentially methylated genes between mouse and human cSCC, with locus specific methylation changes occurring in potential regulatory regions. Dysregulation of DNA methylation, particularly in the Filip1l gene, appears to contribute to the silencing of tumor suppressor genes in both murine and human cSCC.
Article
Medical Laboratory Technology
Farizky Martriano Humardani, Lisa Thalia Mulyanata, Sulistyo Emantoko Dwi Putra
Summary: Cancer-associated necrosis and dietary exposure can both lead to the release of cfDNA, which contains specific methylation patterns in adipose tissue that can be used as a marker for future risk of developing T2DM. Analyzing cfDNA from adipose tissue is superior to using PBL and whole blood samples for reflecting tissue pathology.
CLINICA CHIMICA ACTA
(2023)
Article
Cell Biology
Kevin A. Murach, Cory M. Dungan, Ferdinand von Walden, Yuan Wen
Summary: Research reveals that satellite cell-derived myonuclei have distinct epigenetic characteristics and contributions during muscle adaptation. By comparing myonucleus-specific methylation profiling, it is found that satellite cell-derived myonuclei may preferentially supply specific ribosomal proteins to growing myofibers and retain an epigenetic memory of prior stem cell identity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Geriatrics & Gerontology
Hao Xie, Xin Liu, Qing Zhou, Teng Huang, Lu Zhang, Jia Gao, Yuhan Wang, Yanjun Liu, Tong Yan, Shu Zhang, Cong-Yi Wang
Summary: Aging is a complex health problem associated with various chronic diseases. In adipose tissue, age-related changes result in metabolic dysfunction, inflammation, and cellular senescence. DNA methylation plays a role in regulating metabolic function and the beiging process in adipocytes. Understanding the impact of DNAm on adipose aging is important for developing epigenetic therapeutic strategies in clinical settings.
Review
Geriatrics & Gerontology
Haihua Tian, Zhenyu Hu, Chuang Wang
Summary: Aging is a complex health problem associated with a high risk of chronic diseases, with age-related turnover in adipose tissue being a major contributor to metabolic syndromes and shortened lifespan. DNA methylation regulates cellular senescence, metabolic function and inflammation in adipose tissue, suggesting potential for epigenetic therapeutic strategies against aging-related diseases in clinical settings.
Article
Biochemistry & Molecular Biology
Amber M. Helliwell, Peter A. Stockwell, Christina D. Edgar, Aniruddha Chatterjee, Warren P. Tate
Summary: This study maps the genomic changes in ME/CFS patients during relapse recovery cycles and finds functionally important changes in their DNA methylomes that are associated with metabolic, immune, and inflammatory dysfunctions. These findings provide potential practical applications for strategies to decrease relapse frequency in ME/CFS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Evolutionary Biology
Hannah Watson, Daniel Powell, Pablo Salmon, Arne Jacobs, Caroline Isaksson
Summary: Urbanization drives phenotypic changes with differences in DNA methylation patterns between urban and forest-dwelling birds, particularly in the liver. Differentially methylated genomic sites in the liver are associated with regulatory regions and expressed genes, while blood shows under-representation of regulatory regions and nonexpressed genes in differentially methylated sites.
EVOLUTIONARY APPLICATIONS
(2021)
Article
Cell Biology
Dongxue Ding, Chunrong Wang, Zhao Chen, Kun Xia, Beisha Tang, Rong Qiu, Hong Jiang
Summary: This study investigated the perturbation of DNA methylation status and its impact on gene expression in ATXN3 with (CAG)(n) expansion by analyzing DNA methylation patterns in SCA3/MJD and WT mouse cerebellum. The research identified differentially methylated regions (DMRs) involved in various pathways, and highlighted En1 and Nkx2-1 as DNA methylation-regulated genes associated with the pathogenesis of SCA3/MJD. These findings suggest potential targets for further understanding the molecular mechanisms underlying this disease.
Review
Biochemistry & Molecular Biology
Fabio Coppede, Utsa Bhaduri, Andrea Stoccoro, Vanessa Nicoli, Eleonora Di Venere, Giuseppe Merla
Summary: The central objective of combining discovery science with biomedical applications is to benefit patients and reduce the global disease burden. The conversion of DNA methylation (DNAm) from a biological process to a diagnostic tool is a prime example of this transformation, which took nearly a century. In recent years, DNAm studies have been standardized in clinical applications more than ever before, offering great potential for diagnosing various diseases associated with epigenetic alterations. DNAm detection plays a role in noninvasive prenatal testing and indicates prenatal risk factors in very preterm infants. In the field of cancer, liquid biopsy-based DNAm profiling provides valuable epigenetic biomarkers for early-stage diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Qiu Chen, Yan Wang, Zinan Li, Cong Long, Qian Zhou, Suwen Li
Summary: Obesity is a common metabolic disease caused by the interplay of genetic and environmental factors. Insulin resistance and obesity are closely related, and DNA methylation modification is an important epigenetic regulation method associated with their emergence. Adipose tissue plays a role in obesity and insulin resistance and affects their development through its DNA methylation. Understanding the research on DNA methylation, insulin resistance, and obesity can help identify new targets for clinical prevention and treatment of obesity.
Review
Endocrinology & Metabolism
Sun H. Kim, Ipsa Arora, Daniel S. Hsia, William C. Knowler, Erin LeBlanc, Eleftherios Mylonakis, Richard Pratley, Anastassios G. Pittas
Summary: There is evidence suggesting that infection with SARS-CoV-2 can lead to long-term sequelae, including diabetes. This mini-review examines the evolving literature on new-onset diabetes after COVID-19 (NODAC). Current evidence suggests that COVID-19 increases the risk of developing diabetes, but the exact causality is uncertain due to study limitations and the evolving nature of the pandemic. COVID-19 may have direct and indirect effects on pancreatic beta-cell function and insulin sensitivity.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Cell Biology
Zhiguang Ping, Zhaoyan Guo, Ming Lu, Yanzi Chen, Li Liu
Summary: The methylation level of the CIDEB gene promoter in abdominal subcutaneous adipose tissue and omental adipose tissue may be associated with overweight or obesity in adults. However, there were no statistical differences in the methylation levels of the 16 CpG sites in the CIDEB gene promoter sequence between the normal-weight group and overweight/obesity group.
Article
Biotechnology & Applied Microbiology
Miriama Stiavnicka, Aurelie Chaulot-Talmon, Jean-Philippe Perrier, Petr Hosek, David A. Kenny, Patrick Lonergan, Helene Kiefer, Sean Fair
Summary: This study found that sperm DNA methylation status at specific CpG sites is related to bull fertility and identified seven differently methylated genes in the sperm of subfertile bulls that may lead to altered gene expression and potentially influence embryo development.
Article
Clinical Neurology
Emily W. Flanagan, Jasper Most, Nicholas T. Broskey, Abby D. Altazan, Robbie A. Beyl, Sarah K. Keadle, Kimberly L. Drews, Prachi Singh, Leanne M. Redman
Summary: Increasing sleep duration during pregnancy is associated with improved fasting glucose levels and lower energy intake, leading to potential benefits in cardiometabolic health. However, changes in sleep duration did not significantly affect plasma markers, energy intake, or weight gain.
Review
Immunology
Pooja Budhiraja, Bruce Kaplan, Mohamad Kalot, Abdallah El Alayli, Ahmad Dimassi, Harini A. Chakkera, Raymond Heilman, Audrene S. Edwards, Reem A. Mustafa
Summary: The study examines the strength of evidence from randomized controlled trials (RCTs) on kidney transplantation published in the last decade using the fragility index (FI). The findings suggest that the interpretation of results based solely on a significance level of 0.05 may be insufficient due to methodological limitations in the included trials.
Article
Genetics & Heredity
Xianfeng Chen, Zhifu Sun
Summary: This study utilized a novel lncRNA discovery pipeline and RNA-seq data resources to systematically discover novel lincRNAs in 30 major human tissue types, which defined the characteristics of each tissue type and demonstrated their reliability and tissue-specific expression. Tissue-specific genes were identified for each major tissue type, providing insight into each respective tissue's unique function and expanding interpretation of non-coding SNPs from genome-wide association studies.
Article
Surgery
Harini A. Chakkera, Bruce Kaplan, Pooja Budhiraja, Senaida Behmen, Dennis Fitz-Patrick, Richard J. Butterfield
Summary: This study evaluated the association and discriminative capacity of VO2peak and EPTS score on all-cause mortality post-kidney transplant. Results showed that lower pre-transplant VO2peak and higher EPTS score increased the risk of post-transplant mortality; and among low-risk patients, those with lower VO2peak had significantly higher mortality risk.
CLINICAL TRANSPLANTATION
(2022)
Article
Transplantation
Matthew R. D'Costa, Massini A. Merzkani, Aleksandar Denic, Aidan F. Mullan, Joseph J. Larson, Walter K. Kremers, Walter D. Park, Mariam P. Alexander, Harini A. Chakkera, Sandra J. Taler, Stephen J. Erickson, Mark D. Stegall, Naim Issa, Andrew D. Rule
Summary: Nephrolithiasis in living kidney donors can be evaluated for risk based on baseline clinical, imaging, and biopsy characteristics. Long-term follow-up shows that there is no increased risk of kidney complications in select stone formers after donation.
TRANSPLANTATION DIRECT
(2022)
Article
Transplantation
Massini A. Merzkani, Andrew J. Bentall, Byron H. Smith, Xiomara Benavides Lopez, Matthew R. D'Costa, Walter D. Park, Walter K. Kremers, Naim Issa, Andrew D. Rule, Harini Chakkera, Kunam Reddy, Hasan Khamash, Hani M. Wadei, Martin Mai, Mariam P. Alexander, Hatem Amer, Aleksandra Kukla, Mireille El Ters, Carrie A. Schinstock, Manish J. Gandhi, Raymond Heilman, Mark D. Stegall
Summary: This study aimed to assess risk factors for specific causes of graft loss after kidney transplantation. The results identified two distinct high-risk populations: a younger group with graft failure (GF) due to alloimmunity, and an older, commonly diabetic group with death with a functioning graft (DWFG) and GF due to a mixture of causes, many of which were nonalloimmune. Individualized management is needed to improve long-term renal allograft survival in the latter group.
TRANSPLANTATION DIRECT
(2022)
Article
Cardiac & Cardiovascular Systems
Naima Covassin, Prachi Singh, Shelly K. McCrady-Spitzer, Erik K. St Louis, Andrew D. Calvin, James A. Levine, Virend K. Somers
Summary: Sleep deficiency combined with ad libitum food intake promotes excess energy intake without affecting energy expenditure. Weight gain, especially central accumulation of fat, indicates that sleep loss predisposes individuals to abdominal visceral obesity.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2022)
Article
Physiology
Jacqueline K. Limberg, Sarah E. Baker, Humphrey G. Petersen-Jones, Winston Guo, An Huang, Michael D. Jensen, Prachi Singh
Summary: This study found that intermittent hypoxia increases plasma ET-1 and FFA concentrations, but bosentan can attenuate the increase in FFA. Bosentan does not affect cellular receptors and proteins involved in lipolysis in adipose tissue.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Yun Zhang, Hao Sun, Aishwarya Mandava, Brian D. Aevermann, Tobias R. Kollmann, Richard H. Scheuermann, Xing Qiu, Yu Qian
Summary: In this study, we developed a novel computational immunology analysis pipeline called FastMix, which integrates flow cytometry, bulk transcriptomics, and clinical covariates to identify cell type-specific gene expression signatures and biomarker genes. FastMix addresses the challenges of integrating gene expression and flow cytometry analysis through a linear mixed effects model (LMER) and a moment-based estimator, which reduces bias in parameter estimation and improves efficiency. The FastMix pipeline also includes an advanced flow cytometry data analysis method called DAFi for identifying cell populations of interest and their characteristics. Simulation studies and validation using real data showed that FastMix outperformed competing methods in terms of smaller type I/II errors and producing additional interesting findings.
Article
Endocrinology & Metabolism
Prachi Singh, Robbie A. Beyl, Jacqueline M. Stephens, Robert C. Noland, Allison J. Richard, Anik Boudreau, R. Caitlin Hebert, Eric Ravussin, Josiane L. Broussard, Marie-Pierre St-Onge, Kara L. Marlatt
Summary: The aim of this study was to investigate the effect of sleep restriction on insulin sensitivity and energy metabolism in postmenopausal women. The results showed that four nights of sleep restriction reduced insulin sensitivity and fat oxidation in postmenopausal women, highlighting the significant role of insufficient sleep in metabolic dysfunction following menopause.
Review
Biochemistry & Molecular Biology
Sanah S. Munir, Fatima H. Sert H. Kuniyoshi, Prachi Singh, Naima Covassin
Summary: Obstructive sleep apnea (OSA) is a common sleep disorder and a known risk factor for cardiovascular diseases, including hypertension. The mechanisms involved in OSA-induced hypertension are multifactorial, and the gut microbiome is gaining attention as a potential factor. Perturbations in the gut microbiota have been linked to various disorders, and evidence suggests that gut dysbiosis may promote hypertension development in OSA. This review highlights the current research on the implications of altered gut microbiota for hypertension risk in OSA and potential therapeutic considerations.
Article
Biochemistry & Molecular Biology
Jiaxin Hu, Prachi Singh, Jingrui Li, Jing Zhang, Fei Li, Hehe Zhang, Jiang Xie
Summary: The imbalance between Smad3 and MRTF is associated with cellular senescence and pulmonary arterial remodeling induced by PI hypoxia, providing a potential therapeutic target for patients with sleep apnea and chronic obstructive pulmonary disease.
Article
Genetics & Heredity
Zhifu Sun, Saurabh Behati, Panwen Wang, Aditya Bhagwate, Samantha McDonough, Vivian Wang, William Taylor, Julie Cunningham, John Kisiel
Summary: Whole-genome methylation sequencing provides both DNA methylation and structural variant information. However, there is limited data on the reliability of obtaining this information simultaneously from low-input DNA using different library preparation and sequencing protocols. This study compared the performance of three protocols (EM-sequencing, QIA-sequencing, and Swift-sequencing) on CpG methylation measurement, SNV, and CNV detection. Results showed that at low DNA input, EM-sequencing was superior in most metrics except for CNV detection. EM-sequencing captured the highest number of CpGs and true SNVs, making it suitable for detecting methylation, SNVs, and CNVs from low-input DNA in a single sequencing.
Review
Medicine, General & Internal
Edward C. Mader, Annie Cielo L. Mader, Prachi Singh
Summary: Chronic sleep deficiency (CSD) poses a threat to physical health, mental well-being, and social functioning. Behaviorally induced CSD, now called insufficient sleep syndrome (ISS), is considered a form of central hypersomnolence disorder, with a global rise in voluntary sleep curtailment potentially making ISS the leading cause of CSD. However, ISS is not getting enough attention in contemporary medical practice and public health programs, and it is crucial to prioritize ISS as a public health issue in order to develop effective educational programs and regulations.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Physiology
Prachi Singh, Naima Covassin, Kara Marlatt, Kishore M. Gadde, Steven B. Heymsfield
Summary: This article discusses the role of obesity and sex hormones in determining cardiovascular disease risk, emphasizing the importance of gender differences in cardiovascular risk and the differences in body fat distribution between men and women.
COMPREHENSIVE PHYSIOLOGY
(2022)
