Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Microbiology
Andras Kallai, Marta Kelemen, Noemi Molnar, Adrienn Tropotei, Balazs Hauser, Zsolt Ivanyi, Janos Gal, Erzsebet Ligeti, Katalin Kristof, Akos M. Lorincz
Summary: The study developed a flow cytometry-based antibiotic susceptibility testing (AST) method, which can produce reliable results a workday earlier than the traditional microdilution method. Results showed that the method is able to detect bacterial growth accurately after 4 hours of incubation, with high consistency compared to the microdilution method.
MICROBIOLOGY SPECTRUM
(2021)
Review
Immunology
Jeroen Bok, Regina W. Hofland, Carlton A. Evans
Summary: Whole blood mycobacterial growth assays (WBMGA) have the potential for evaluating tuberculosis vaccines and identifying individuals at risk of tuberculosis. Studies have shown significant associations between WBMGA results and correlates of tuberculosis susceptibility, but no direct evidence on whether WBMGA results can predict actual susceptibility to tuberculosis infection or disease is available. Further optimization, standardization, and prospective studies are recommended to determine if WBMGA can predict susceptibility to tuberculosis disease.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Kamal Singh, Swati Sharma, Tuhina Banerjee, Ankush Gupta, Shampa Anupurba
Summary: This study aimed to determine and compare the minimum inhibitory concentration (MIC) of linezolid and clofazimine in confirmed extensively drug-resistant tuberculosis (XDR-TB) clinical isolates using agar dilution and resazurin microtiter assay (REMA) methods. The results showed that REMA method was more suitable due to its shorter turnaround time. Mutations in rplC and Rv0678 genes were identified as the reasons for drug resistance against linezolid and clofazimine, respectively.
Article
Microbiology
Rundong Tan, Anqi Yu, Ziming Liu, Ziqi Liu, Rongfeng Jiang, Xiaoli Wang, Jialin Liu, Junhui Gao, Xinjun Wang
Summary: MIC is crucial for bacterial detection in infections, and predicting MIC accurately using genome sequencing and machine learning algorithms can improve clinical efficacy and antibiotic use.
FRONTIERS IN MICROBIOLOGY
(2021)
Editorial Material
Obstetrics & Gynecology
Margaux Jegaden, Elodie Debras, Anne-Gaelle Pourcelot, Perrine Capmas, Herve Fernandez
Summary: This article introduces a new minimally invasive surgical technique, vNOTES ovarian drilling, for fertility diagnosis in patients with polycystic ovary syndrome. This technique is characterized by its minimally invasive nature, absence of scarring, and good tolerance, allowing simultaneous ovarian drilling and fertility diagnosis. The new technique is convenient to perform and associated with minimal adverse effects on patients.
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2022)
Article
Infectious Diseases
Radka Hulankova
Summary: There is currently no internationally accepted, standardized method for determining the minimum inhibitory concentration (MIC) of natural substances like essential oils. This study aimed to compare MIC values obtained from different studies that used various culture media. The results showed that the MIC values for cinnamon essential oil varied depending on the culture media used, with the median values ranging from 465 to 517 μg/mL. The MIC values for oregano essential oil were significantly lower in Mueller-Hinton Broth (MHB) compared to highly nutritious media. Additionally, the lag phase of bacterial growth in the presence of oregano essential oil was much shorter in MHB compared to other media. Therefore, MHB is not recommended for comparing the antimicrobial properties of different essential oils.
Article
Chemistry, Analytical
Aleksandr Gorst, Kseniya Zavyalova, Aleksandr Mironchev
Summary: The article describes a near-field sensor model for non-invasive glucose monitoring, with specific design and high penetration capabilities. Through experimental verification and numerical modeling, the sensor's high accuracy in measuring glucose concentration is demonstrated.
Article
Medicine, General & Internal
Khayal Gasimli, Lisa Wilhelm, Sven Becker, Rudy Leon De Wilde, Morva Tahmasbi Rad
Summary: This article reviews the available data and different opinions on the ideal management of early-stage cervical cancer, highlighting the controversy and biases involved.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Wei Feng, Lei Zhang, Qian Yuan, Yu Wang, Pu Yao, Peiyuan Xia, Fengjun Sun
Summary: The study demonstrated that sub-minimal inhibitory concentration of ceftazidime can reduce the pathogenicity of UPEC by inhibiting adhesion ability, biofilm formation, swimming and swarming motilities, and decreasing hemolytic activity and cytotoxicity. CAZ also affects UPEC virulence and biofilm formation by downregulating important genes, leading to reduced adhesion to human bladder epithelial cells and less severe UTI symptoms.
MICROBIAL PATHOGENESIS
(2021)
Article
Food Science & Technology
Joana Gomes, Joana Barbosa, Paula Teixeira
Summary: This study aimed to evaluate the minimum inhibitory concentrations of three natural antimicrobial compounds against 37 microorganisms using agar dilution and drop diffusion methods. The results showed that lower pH values and the agar dilution method generally led to lower minimum inhibitory concentrations. However, the inhibition of different compounds varied under different conditions.
Article
Critical Care Medicine
Nakwon Kwak, Jake Whang, Jeong Seong Yang, Taek Soo Kim, Sung A. Kim, Jae-Joon Yim
Summary: Clofazimine MIC values varied widely in clinical isolates from NTM-PD patients, and negative conversion of sputum culture with clofazimine use was associated with lower MIC values. Clofazimine use could be considered in patients with NTM-PD when the MIC value is <= 0.25 mg/L.
Article
Health Care Sciences & Services
Clara Grazian
Summary: Antimicrobial resistance poses a significant threat to global public health. Researchers are using whole-genome sequencing to develop personalized treatments and improve prediction of resistance and treatment recommendations. Robust methods are needed to identify resistance-associated mutations.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Renjie Du, Yuejun Qu, Min Zhao, Yanhong Liu, Phoebe X. Qi, Xingbin Sun
Summary: This study utilizes logistic modeling to predict the minimum inhibitory concentration (MIC) of olive leaf extract (OLE) against Listeria monocytogenes. The MIC value estimated through logistic modeling, called MIC-PDA, shows a potential to reduce the concentration and has been validated. The logistic modeling method is also verified by inhibition data of OLE against the growths of Escherichia coli O157:H7 and Salmonella enteritidis, demonstrating its potential in the food industry as an antimicrobial.
Article
Multidisciplinary Sciences
Azarmidokht Sheini, Marzieh Dadkhah Aseman, Mohammad Mahdi Bordbar
Summary: A simple origami based microfluidic device was introduced for point of need detection of blood cyanide concentration in fire victims, offering a cost-effective and time-saving alternative to conventional laboratory methods. The device was successfully applied to determine cyanide ions in the blood samples of fire survivors with high accuracy.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Martina Avbelj, Jure Zupan, Luka Kranjc, Peter Raspor
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2015)
Article
Infectious Diseases
Zorica Tomicic, Jure Zupan, Tadeja Matos, Peter Raspor
Article
Biotechnology & Applied Microbiology
Ruzica Tomicic, Zorica Tomicic, Peter Raspor
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2017)
Article
Multidisciplinary Sciences
Miha Tome, Jure Zupan, Zorica Tomicic, Tadeja Matos, Peter Raspor
Article
Biotechnology & Applied Microbiology
Neza Cadez, Jure Zupan, Peter Raspor
FEMS YEAST RESEARCH
(2010)
Article
Food Science & Technology
Matjaz Dezelak, Mekonnen M. Gebremariam, Neza Cadez, Jure Zupan, Peter Raspor, Martin Zarnkow, Thomas Becker, Iztok Joze Kosir
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2014)
Article
Agriculture, Multidisciplinary
Jure Zupan, Martina Avbelj, Bojan Butinar, Janez Kosel, Matej Sergan, Peter Raspor
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2013)
Article
Biochemical Research Methods
Jure Zupan, Peter Raspor
JOURNAL OF MICROBIOLOGICAL METHODS
(2008)
Article
Biochemical Research Methods
Jure Zupan, Jan Mavri, Peter Raspor
JOURNAL OF MICROBIOLOGICAL METHODS
(2009)
Article
Biochemistry & Molecular Biology
Jure Zupan, Peter Raspor
Article
Forestry
Ruzica Tomicic, Zorica Tomicic, Nejc Thaler, Miha Humar, Peter Raspor
WOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Environmental Sciences
Jasmina Lazarevic, Ivana Cabarkapa, Sladana Rakita, Maja Banjac, Zorica Tomicic, Dubravka Skrobot, Goran Radivojevic, Bojana Kalenjuk Pivarski, Dragan Tesanovic
Summary: This study evaluated the safety parameters, nutritional value, and sensory profile of spiny-cheek crayfish meat from the Danube River in Serbia. The results showed that the meat was microbiologically safe and had high nutritional quality, with a good fatty acid and amino acid profile, as well as high levels of essential minerals.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Zorica Tomicic, Ruzica Tomicic, Sonja Smole Mozina, Franz Bucar, Ivana Turek, Peter Raspor
Summary: This study evaluated the antifungal and anti-adhesion potential of 15 plant extracts, essential oils, and chemical compounds against Candida and Pichia species. The results showed that cinnamon and oregano essential oils had the highest inhibitory effect, while Sedum roseum extract exhibited the strongest anti-adhesion effect. Additionally, the essential oils of cinnamon, oregano, savory, and thyme showed promising activity against the formation of biofilm by Candida species.
JOURNAL OF FOOD AND NUTRITION RESEARCH
(2022)