Article
Agriculture, Multidisciplinary
Wenxuan Huang, Yan Huo, Shaochen Yang, Mingjia Liu, Han Li, Man Zhang
Summary: In this paper, an improved YOLOv5s algorithm was proposed to implement the recognition and counting system of Laodelphax striatellus (SBPH). The model was enhanced by adding channel attention and spatial attention, as well as incorporating the Convolutional Block Attention Module (CBAM) and Adaptively Spatial Feature Fusion (ASFF). Experimental results showed that the improved model had a similar volume to YOLOv5s, but achieved approximately 4% higher mAP than YOLOv5s and 2% higher mAP than YOLOv5x. Additionally, an APP was developed using Android Studio to enable mobile image recognition and counting.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jun Zhang, Chengling Lai, Xuping Shentu, Peiying Hao, Kun Pang, Xiaoping Yu
Summary: A rapid and accurate detection method for the yeast-like symbionts (YLS) of BPH was established using ddPCR. The limits of detection for the two YLS species were 1.3 copies/μL and 1.2 copies/μL, respectively. This method exhibited good sensitivity, specificity, and repeatability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Shengli Jing, Jingang Xu, Hengmin Tang, Peng Li, Bin Yu, Qingsong Liu
Summary: This review explores the roles of sRNAs in rice-BPH interactions and highlights recent research progress in unraveling the mechanism of cross-kingdom RNA interference between host plants and insects, as well as the application of this interference in pest management of crops including rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Shaoqi Zhan, Zhentao Sheng, Yiming Liu, Ke Xu, Jiahui Wang, Weixing Cao, Yongchao Tian, Jun Ni
Summary: The study found that the trapping rate of Laodelphax striatellus was highest with a linear blue light source at 460nm and significantly affected by light intensity. Compared to monochromatic light sources, combined color light sources significantly increased the trapping rate of Laodelphax striatellus, with blue-green light showing the best improvement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Katherine A. Davies, Stephen R. Welch, Teresa E. Sorvillo, JoAnn D. Coleman-McCray, Maria Laura Martin, Julia M. Brignone, Joel M. Montgomery, Christina F. Spiropoulou, Jessica R. Spengler
Summary: This study investigated the suitability of potential reference genes in laboratory animals commonly used for studying viral hemorrhagic fever infection. The most stable reference gene pairs were identified for each rodent species, and a pan-rodent Ppia assay capable of detecting Ppia across multiple rodent species was developed.
SCIENTIFIC REPORTS
(2023)
Article
Entomology
Yuanyuan Zhang, Gang Xu, Yu Jiang, Chao Ma, Guoqing Yang
Summary: This study investigated the potential effects of sublethal concentrations of imidacloprid on the small brown planthopper (SBPH) and found that it could increase the fecundity of SBPH. Additionally, the impact of insecticides on the transmission of plant viruses by insects should be considered.
Article
Microbiology
Marta Luigi, Ariana Manglli, Antonio Tiberini, Sabrina Bertin, Luca Ferretti, Anna Taglienti, Francesco Faggioli, Laura Tomassoli
Summary: In 2020, a test performance study was conducted in the H2020 Valitest project to detect the specific tomato brown rugose fruit virus (ToBRFV). Five molecular tests were found to be reliable in detecting ToBRFV and included in the EPPO standard PM 7/146-Diagnostics. A total of 34 laboratories from 18 countries participated in the study and evaluated the diagnostic parameters of each test.
Review
Biochemistry & Molecular Biology
Eui-Joon Kil, Donghun Kim
Summary: In this review, the destructive nature of the small brown planthopper, Laodelphax striatellus, and its transmission of viruses causing significant loss in rice production are discussed. Research on vector biology, interactions between vectors and pathogens, as well as omics approaches have been conducted to explore novel strategies for control. The roles of saliva proteins, microbial community, insecticide resistance, RSV classification, host range and symptoms, genome composition and virus-derived proteins, distribution, interactions, resistance, and control are explored to propose future directions for integrated pest management.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agronomy
Chengye Lu, Daoran Jin, Lujie Zhang, Gang Lu, Yinghua Ji, Yijun Zhou, Yunyue Wang, Shuo Li
Summary: This study demonstrates that transgenic rice plants expressing viral glycoproteins can interfere with the acquisition and transmission of Rice stripe virus (RSV) by the small brown planthopper (SBPH). The soluble fraction (NSvs2-N-S) of the viral glycoprotein NSvs2-N was transformed into rice calli, resulting in transgenic plants with the exogenous gene. Feeding SBPH on the transgenic plants before RSV-infected rice or feeding the insects on RSV-infected transgenic plants inhibited virus acquisition by the vectors and reduced subsequent viral titers. Immunofluorescence labeling confirmed the inhibition of viral infection in the insect midgut after SBPH feeding on transgenic plants. In vitro experiments using RSV-infected insect cells further supported the role of NSvc2-N-S in reducing viral infection. The findings suggest that NSvs2-N-S transgenic plants have a negative effect on RSV transmission by insect vectors and provide a novel and effective approach to control plant viral diseases.
PEST MANAGEMENT SCIENCE
(2022)
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Agriculture, Multidisciplinary
Yan Zhi-yong, Zhao Mei-sheng, Ma Hua-yu, Liu Ling-zhi, Yang Guang-ling, Geng Chao, Tian Yan-ping, Li Xiang-dong
Summary: Tomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus that poses a severe threat to the tomato industry, having spread to 10 countries in America, Asia, and Europe. A study in Shandong Province, China showed that ToBRFV-SD induced distinct symptoms on various plants, with all tomato cultivars tested highly sensitive to it. A quadruplex RT-PCR system was developed for efficient detection of ToBRFV and differentiation from other important tomato viruses.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Virology
Lu Tong, Xiaofang Chen, Wei Wang, Yan Xiao, Jinting Yu, Hong Lu, Feng Cui
Summary: Alternative splicing (AS) is a common posttranscriptional regulatory event in eukaryotic organisms, and its effects on insect vectors in response to virus infection are not well understood. In this study, third-generation sequencing technology was used to analyze the AS response of the small brown planthopper to rice stripe virus (RSV). It was found that 31.4% of alternatively spliced genes responded to RSV infection, and one of the c-Jun N-terminal kinase genes showed different isoforms that facilitated viral accumulation in the planthoppers. These results suggest that AS events may play a role in the complex regulatory relationships between viruses and insect vectors.
JOURNAL OF VIROLOGY
(2022)
Article
Agronomy
Kun Zhang, Xinjian Zhuang, Hongmei Xu, Xiao Guo, Zhen He, Kai Xu, Fang Liu
Summary: This study cloned and expressed the capsid protein gene of rice stripe virus and developed multiple serology-based detection methods using the prepared polyclonal antibody. These methods can simultaneously identify RSV-infected rice plants and SBPHs in wild field conditions, contributing to the control of RSV in rice planting regions.
Article
Plant Sciences
Qingqing Chen
Summary: The study found that in the high marsh, herbivores may drive trait changes through nutrients, while in the low marsh, nutrients may be the dominant driver for trait changes. The results suggest that ecologically important abiotic and biotic variables such as nutrients and herbivores may have a substantial evolutionary impact on plant populations.
Article
Ecology
Oliver Carroll, Evan Batzer, Siddharth Bharath, Elizabeth T. Borer, Sofia Campana, Ellen Esch, Yann Hautier, Timothy Ohlert, Eric W. Seabloom, Peter B. Adler, Jonathan D. Bakker, Lori Biederman, Miguel N. Bugalho, Maria Caldeira, Qingqing Chen, Kendi F. Davies, Philip A. Fay, Johannes M. H. Knops, Kimberly Komatsu, Jason P. Martina, Kevin S. McCann, Joslin L. Moore, John W. Morgan, Taofeek O. Muraina, Brooke Osborne, Anita C. Risch, Carly Stevens, Peter A. Wilfahrt, Laura Yahdjian, Andrew S. MacDougall
Summary: Nutrient additions can increase aboveground biomass production but also decrease its stability, with nitrogen and phosphorus disproportionately increasing interannual variability.
Article
Biodiversity Conservation
Qingqing Chen, Shaopeng Wang, Eric W. Seabloom, Andrew S. MacDougall, Elizabeth T. Borer, Jonathan D. Bakker, Ian Donohue, Johannes M. H. Knops, John W. Morgan, Oliver Carroll, Mick Crawley, Miguel N. Bugalho, Sally A. Power, Anu Eskelinen, Risto Virtanen, Anita C. Risch, Martin Schutz, Carly Stevens, Maria C. Caldeira, Sumanta Bagchi, Juan Alberti, Yann Hautier
Summary: Nutrients and herbivores have separate effects on aboveground biomass stability in grasslands, with nutrient addition increasing community composition changes over time and herbivore exclusion decreasing spatial asynchrony. However, their interaction has a weak positive effect on stability by increasing spatial asynchrony. Understanding the processes operating at different spatial scales can contribute to the conservation and management of ecosystems.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Medicine, General & Internal
Yong-Hua Chen, Qing-Qing Chen, Chun-Lin Wang
Summary: This case report presents a rare case of TAIRS in an 11-year-old female patient with an INSR heterozygous mutation treated with a combination of pioglitazone and flutamide for 5 years. The treatment regimen effectively reduced hemoglobin A1c, fasting insulin, and androgen levels.
WORLD JOURNAL OF CLINICAL CASES
(2022)
Article
Public, Environmental & Occupational Health
Jun He, Sai Hou, Yue Chen, Jun-Ling Yu, Qing-Qing Chen, Lan He, Jiang Liu, Lei Gong, Xin-Er Huang, Jia-Bing Wu, Hai-Feng Pan, Rong-Bao Gao
Summary: Influenza-like illness is a global public health problem, and a study in Anhui province, China found that school-aged population is the most vulnerable group and co-infection of influenza A and B viruses was detected.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Oncology
Fang Wang, Qingqing Chen, Yuanyuan Zhang, Yinan Chen, Yajing Zhu, Wei Zhou, Xiao Liang, Yunjun Yang, Hongjie Hu
Summary: This study demonstrates the effectiveness of radiomics signature in predicting the recurrence of hepatocellular carcinoma (HCC) and the benefits of postoperative adjuvant transcatheter arterial chemoembolization (PA-TACE). The newly developed radiomics model is a powerful biomarker for predicting recurrence-free survival (RFS) in HCC patients and can identify high-risk patients who would benefit from PA-TACE.
JOURNAL OF HEPATOCELLULAR CARCINOMA
(2022)
Article
Infectious Diseases
Qingqing Chen, Zhencang Zheng, Qingxin Shi, Huijuan Wu, Yuping Li, Cheng Zheng
Summary: Polymicrobial Acinetobacter baumannii bloodstream infections (AB-BSI), caused by multiple microorganisms, account for a significant proportion among all AB-BSI cases. Although it does not affect mortality, polymicrobial AB-BSI is associated with slightly longer hospital stays. Multidrug resistance is associated with the development of polymicrobial AB-BSI but does not directly cause it, while resistance to piperacillin/tazobactam is highly correlated with polymicrobial AB-BSI.
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY
(2022)
Article
Cell Biology
Jiahao Zhu, Yutian Zhao, Gang Wu, Xiaojun Zhang, Qingqing Chen, Bo Yang, Xinwei Guo, Shengjun Ji, Ke Gu
Summary: Esophageal squamous cell carcinoma (ESCC) is closely associated with poor survival, and ferroptosis, an iron-dependent mode of cell death, plays an important role in the biological behavior of ESCC cells. This study aimed to explore the clinical significance of ferroptosis-related long noncoding RNAs (FRLs) in ESCC. Through RNA sequencing and coexpression analysis, a ten-FRL prediction signature was established and validated using multiple databases. The risk model showed promise as a predictor of prognosis and immunotherapeutic response in patients with ESCC.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Immunology
Yi Miao, Jing Zhang, Qingqing Chen, Lingxiao Xing, Tonglu Qiu, Huayuan Zhu, Li Wang, Lei Fan, Wei Xu, Jianyong Li
Summary: Lymphoid malignancy is the most common underlying cause of adult HLH, followed by EBV infection. Based on the observation of bone marrow morphology and EBV load, we developed a diagnostic flow for rapid determination of the triggers for HLH.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Haitao Wang, Jianhua Zhang, Haoqiu Liu, Man Wang, Yan Dong, Yijun Zhou, Sek-Man Wong, Kai Xu, Qiufang Xu
Summary: The rice black-streaked dwarf virus (RBSDV) utilizes phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P-2) to evade autophagic degradation in insects and promote its survival.
Article
Mathematical & Computational Biology
Qing Ye, Yongkang Lang, Zongqu Zhao, Qingqing Chen, Yongli Tang
Summary: Machine learning and privacy protection are closely related. This study proposes a lattice-based ring signature scheme without trapdoors, which has high computational efficiency and is suitable for machine learning scenarios.
COMPUTATIONAL INTELLIGENCE AND NEUROSCIENCE
(2022)
Article
Plant Sciences
Peter A. Wilfahrt, Eric W. Seabloom, Jonathan D. Bakker, Lori Biederman, Miguel N. Bugalho, Marc W. Cadotte, Maria C. Caldeira, Jane A. Catford, Qingqing Chen, Ian Donohue, Anne Ebeling, Nico Eisenhauer, Sylvia Haider, Robert W. Heckman, Anke Jentsch, Sally E. Koerner, Kimberly J. Komatsu, Ramesh Laungani, Andrew MacDougall, Jason P. Martina, Holly Martinson, Joslin L. Moore, Yujie Niu, Timothy Ohlert, Harry Olde Venterink, Devyn Orr, Pablo Peri, Edwin Pos, Jodi Price, Xavier Raynaud, Zhengwei Ren, Christiane Roscher, Nicholas G. Smith, Carly J. Stevens, Lauren L. Sullivan, Michelle Tedder, Pedro M. Tognetti, Ciska Veen, George Wheeler, Alyssa L. Young, Hillary Young, Elizabeth T. Borer
Summary: This study highlights the importance of both resource availability and historical abundance in determining the persistence of species dominance following environmental perturbations. Fertilization and exclusion of vertebrates influence the rate at which species lose dominance, with initial cover playing a significant role. The ability of plant species to maintain dominance following perturbations affects ecosystem functions and community compositions.
JOURNAL OF ECOLOGY
(2023)
Article
Multidisciplinary Sciences
Qingqing Chen, Shaopeng Wang, Elizabeth T. Borer, Jonathan D. Bakker, Eric W. Seabloom, W. Stanley Harpole, Nico Eisenhauer, Ylva Lekberg, Yvonne M. Buckley, Jane A. Catford, Christiane Roscher, Ian Donohue, Sally A. Power, Pedro Daleo, Anne Ebeling, Johannes M. H. Knops, Jason P. Martina, Anu Eskelinen, John W. Morgan, Anita C. Risch, Maria C. Caldeira, Miguel N. Bugalho, Risto Virtanen, Isabel C. Barrio, Yujie Niu, Anke Jentsch, Carly J. Stevens, Daniel S. Gruner, Andrew S. Macdougall, Juan Alberti, Yann Hautier
Summary: Eutrophication has varying effects on different aspects of grassland ecological stability, and different stability measures are largely uncorrelated. This highlights the high dimensionality of stability and provides insights for predicting grassland responses to global environmental change.
NATURE COMMUNICATIONS
(2023)
Article
Public, Environmental & Occupational Health
Miao Lu, Qingqing Chen, Xincheng Qin, Yong Lyu, Zhongqiu Teng, Kun Li, Liang Yu, Xiaojing Jin, Hongwei Chang, Wen Wang, Dayin Hong, Yong Sung, Biao Kan, Lei Gong, Tian Qin
Article
Cell Biology
Chunlin Wang, Qingqing Chen, Ke Yuan, Minfei He, Jianfang Zhu, Yanlan Fang, Jianhong Hu, Qingfeng Yan
Summary: Central precocious puberty (CPP) is a disease that seriously affects the development of a child and is increasing year by year, with limited treatment options. An analysis revealed altered members of metabolic signaling pathways in CPP may serve as potential novel therapeutic targets.