CRISPR-Cas: Adapting to change

Deciphering, Communicating, and Engineering the CRISPR PAM

(2017) Ryan T. Leenay et al.   JOURNAL OF MOLECULAR BIOLOGY

Coupling immunity and programmed cell suicide in prokaryotes: Life-or-death choices

(2016) Eugene V. Koonin et al.   BIOESSAYS

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity

(2016) Rongpeng Li et al.   CELL RESEARCH

Active and adaptiveLegionellaCRISPR-Cas reveals a recurrent challenge to the pathogen

(2016) Chitong Rao et al.   CELLULAR MICROBIOLOGY

Recent Mobility of Casposons, Self-Synthesizing Transposons at the Origin of the CRISPR-Cas Immunity

(2016) Mart Krupovic et al.   Genome Biology and Evolution

Requirements for Pseudomonas aeruginosa Type I-F CRISPR-Cas Adaptation Determined Using a Biofilm Enrichment Assay

(2016) Gary E. Heussler et al.   JOURNAL OF BACTERIOLOGY

Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity

(2016) Chaoyou Xue et al.   MOLECULAR CELL

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

(2016) Adrian G. Patterson et al.   MOLECULAR CELL

CRISPR Immunological Memory Requires a Host Factor for Specificity

(2016) James K. Nuñez et al.   MOLECULAR CELL

CRISPR-Cas Systems Optimize Their Immune Response by Specifying the Site of Spacer Integration

(2016) Jon McGinn et al.   MOLECULAR CELL

Adaptation in CRISPR-Cas Systems

(2016) Samuel H. Sternberg et al.   MOLECULAR CELL

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

(2016) Tim Künne et al.   MOLECULAR CELL

The diversity-generating benefits of a prokaryotic adaptive immune system

(2016) Stineke van Houte et al.   NATURE

Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

(2016) Robert P. Hayes et al.   NATURE

Uncovering Earth’s virome

(2016) David Paez-Espino et al.   NATURE

CRISPR–Cas adaptation: insights into the mechanism of action

(2016) Gil Amitai et al.   NATURE REVIEWS MICROBIOLOGY

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Casposon integration shows strong target site preference and recapitulates protospacer integration by CRISPR-Cas systems

(2016) Pierre Béguin et al.   NUCLEIC ACIDS RESEARCH

DNA motifs determining the accuracy of repeat duplication during CRISPR adaptation inHaloarcula hispanica

(2016) Rui Wang et al.   NUCLEIC ACIDS RESEARCH

Asymmetric positioning of Cas1–2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system

(2016) K.N.R. Yoganand et al.   NUCLEIC ACIDS RESEARCH

Highly efficient primed spacer acquisition from targets destroyed by theEscherichia colitype I-E CRISPR-Cas interfering complex

(2016) Ekaterina Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quorum sensing controls thePseudomonas aeruginosaCRISPR-Cas adaptive immune system

(2016) Nina M. Høyland-Kroghsbo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Continuous genetic recording with self-targeting CRISPR-Cas in human cells

(2016) S. D. Perli et al.   SCIENCE

Molecular recordings by directed CRISPR spacer acquisition

(2016) Seth L. Shipman et al.   SCIENCE

Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein

(2016) S. Silas et al.   SCIENCE

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System

(2016) Donghyun Ka et al.   STRUCTURE

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system

(2016) Raymond H. J. Staals et al.   Nature Communications

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array

(2016) Moran G. Goren et al.   Cell Reports

Anti-cas spacers in orphan CRISPR4 arrays prevent uptake of active CRISPR–Cas I-F systems

(2016) Cristóbal Almendros et al.   Nature Microbiology

Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species

(2016) April Pawluk et al.   Nature Microbiology

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation

(2015) Yunzhou Wei et al.   GENES & DEVELOPMENT

Metagenomic reconstructions of bacterial CRISPR loci constrain population histories

(2015) Christine L Sun et al.   ISME Journal

Two Distinct DNA Binding Modes Guide Dual Roles of a CRISPR-Cas Protein Complex

(2015) Timothy R. Blosser et al.   MOLECULAR CELL

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

CRISPR adaptation biases explain preference for acquisition of foreign DNA

(2015) Asaf Levy et al.   NATURE

Cas9 specifies functional viral targets during CRISPR–Cas adaptation

(2015) Robert Heler et al.   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Mechanism of CRISPR-RNA guided recognition of DNA targets inEscherichia coli

(2015) Paul B.G. van Erp et al.   NUCLEIC ACIDS RESEARCH

DNA targeting by the type I-G and type I-A CRISPR–Cas systems ofPyrococcus furiosus

(2015) Joshua Elmore et al.   NUCLEIC ACIDS RESEARCH

The casposon-encoded Cas1 protein fromAciduliprofundum booneiis a DNA integrase that generates target site duplications

(2015) Alison B. Hickman et al.   NUCLEIC ACIDS RESEARCH

CRISPR interference and priming varies with individual spacer sequences

(2015) Chaoyou Xue et al.   NUCLEIC ACIDS RESEARCH

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

(2015) Daria Vorontsova et al.   NUCLEIC ACIDS RESEARCH

Regulation of the Type I-F CRISPR-Cas system by CRP-cAMP and GalM controls spacer acquisition and interference

(2015) Adrian G. Patterson et al.   NUCLEIC ACIDS RESEARCH

Sequences spanning the leader-repeat junction mediate CRISPR adaptation to phage in Streptococcus thermophilus

(2015) Yunzhou Wei et al.   NUCLEIC ACIDS RESEARCH

Different genome stability proteins underpin primed and naïve adaptation inE. coliCRISPR-Cas immunity

(2015) Ivana Ivančić-Baće et al.   NUCLEIC ACIDS RESEARCH

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

(2015) Clare Rollie et al.   eLife

Casposons: a new superfamily of self-synthesizing DNA transposons at the origin of prokaryotic CRISPR-Cas immunity

(2014) Mart Krupovic et al.   BMC BIOLOGY

Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting

(2014) Gregory W. Goldberg et al.   NATURE

Evolution of adaptive immunity from transposable elements combined with innate immune systems

(2014) Eugene V. Koonin et al.   NATURE REVIEWS GENETICS

CRISPR–Cas systems: beyond adaptive immunity

(2014) Edze R. Westra et al.   NATURE REVIEWS MICROBIOLOGY

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Haloarcula hispanica CRISPR authenticates PAM of a target sequence to prime discriminative adaptation

(2014) M. Li et al.   NUCLEIC ACIDS RESEARCH

Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system

(2014) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer

(2014) Corinna Richter et al.   NUCLEIC ACIDS RESEARCH

Pervasive generation of oppositely oriented spacers during CRISPR adaptation

(2014) Sergey Shmakov et al.   NUCLEIC ACIDS RESEARCH

Degenerate target sites mediate rapid primed CRISPR adaptation

(2014) P. C. Fineran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Adaptation in bacterial CRISPR-Cas immunity can be driven by defective phages

(2014) Alexander P. Hynes et al.   Nature Communications

Remarkable Mechanisms in Microbes to Resist Phage Infections

(2014) Ron L. Dy et al.   Annual Review of Virology

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus

(2013) Sofia Lemak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A CRISPR/Cas system mediates bacterial innate immune evasion and virulence

(2013) Timothy R. Sampson et al.   NATURE

Revenge of the phages: defeating bacterial defences

(2013) Julie E. Samson et al.   NATURE REVIEWS MICROBIOLOGY

Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2

(2013) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process

(2013) Ming Li et al.   NUCLEIC ACIDS RESEARCH

Identification, structural, and biochemical characterization of a group of large Csn2 proteins involved in CRISPR-mediated bacterial immunity

(2013) Kwang-Hoon Lee et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

High-throughput analysis of type I-E CRISPR/Cas spacer acquisition inE. coli

(2013) Ekaterina Savitskaya et al.   RNA Biology

CRISPR-Cas systems preferentially target the leading regions of MOBFconjugative plasmids

(2013) Edze R. Westra et al.   RNA Biology

CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants ofEscherichia coli

(2013) César Díez-Villaseñor et al.   RNA Biology

Strong bias in the bacterial CRISPR elements that confer immunity to phage

(2013) David Paez-Espino et al.   Nature Communications

CRISPR-Cas and restriction–modification systems are compatible and increase phage resistance

(2013) Marie-Ève Dupuis et al.   Nature Communications

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

Live virus-free or die: coupling of antivirus immunity and programmed suicide or dormancy in prokaryotes

(2012) Kira S Makarova et al.   Biology Direct

Characterization of the CRISPR/Cas Subtype I-A System of the Hyperthermophilic Crenarchaeon Thermoproteus tenax

(2012) A. Plagens et al.   JOURNAL OF BACTERIOLOGY

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

The highly dynamic CRISPR1 system ofStreptococcus agalactiaecontrols the diversity of its mobilome

(2012) Maria-José Lopez-Sanchez et al.   MOLECULAR MICROBIOLOGY

Selective and hyperactive uptake of foreign DNA by adaptive immune systems of an archaeon via two distinct mechanisms

(2012) Susanne Erdmann et al.   MOLECULAR MICROBIOLOGY

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

(2012) Joe Bondy-Denomy et al.   NATURE

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

In Vivo Protein Interactions and Complex Formation in the Pectobacterium atrosepticum Subtype I-F CRISPR/Cas System

(2012) Corinna Richter et al.   PLoS One

The CRISPR Associated Protein Cas4 Is a 5′ to 3′ DNA Exonuclease with an Iron-Sulfur Cluster

(2012) Jing Zhang et al.   PLoS One

Memory of viral infections by CRISPR-Cas adaptive immune systems: Acquisition of new information

(2012) Peter C. Fineran et al.   VIROLOGY

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Novel Virulence Gene and Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Multilocus Sequence Typing Scheme for Subtyping of the Major Serovars ofSalmonella entericasubsp.enterica

(2011) Fenyun Liu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Self-targeting by CRISPR: gene regulation or autoimmunity?

(2010) Adi Stern et al.   TRENDS IN GENETICS

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex

(2009) Caryn R. Hale et al.   CELL

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE

Virus Population Dynamics and Acquired Virus Resistance in Natural Microbial Communities

(2008) A. F. Andersson et al.   SCIENCE