期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 1, 页码 367-381出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkw1151
关键词
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资金
- Department of Biotechnology (DBT) [BT/08/IYBA/2014-1 5, BT/406/NE/UEXCEL/2013, BT/PR5511/MED/29/631/2 012, BT/341/NE/TBP/2012]
- Science and Engineering Research Board (SERB) [YSS/2014/000286]
CRISPR-Cas system epitomizes prokaryote-specific quintessential adaptive defense machinery that limits the genome invasion of mobile genetic elements. It confers adaptive immunity to bacteria by capturing a protospacer fragment from invading foreign DNA, which is later inserted into the leader proximal end of CRIPSR array and serves as immunological memory to recognize recurrent invasions. The universally conserved Cas1 and Cas2 form an integration complex that is known tomediate the protospacer invasion into the CRISPR array. However, the mechanism by which this protospacer fragment gets integrated in a directional fashion into the leader proximal end is elusive. Here, we employ CRISPR/dCas9 mediated immunoprecipitation and genetic analysis to identify Integration Host Factor (IHF) as an indispensable accessory factor for spacer acquisition in Escherichia coli. Further, we show that the leader region abutting the first CRISPR repeat localizes IHF and Cas1-2 complex. IHF binding to the leader region induces bending by about 120. that in turn engenders the regeneration of the cognate binding site for protospacer bound Cas1-2 complex and brings it in proximity with the first CRISPR repeat. This appears to guide Cas1-2 complex to orient the protospacer invasion towards the leaderrepeat junction thus driving the integration in a polarized fashion.
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