Crystal structure of the human lysosomal mTORC1 scaffold complex and its impact on signaling

Dynamic relocation of the TORC1–Gtr1/2–Ego1/2/3 complex is regulated by Gtr1 and Gtr2

(2016) Shintaro Kira et al.   MOLECULAR BIOLOGY OF THE CELL

Dynamics of mTORC1 activation in response to amino acids

(2016) Maria Manifava et al.   eLife

Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling

(2015) Katie Powis et al.   CELL RESEARCH

BORC, a Multisubunit Complex that Regulates Lysosome Positioning

(2015) Jing Pu et al.   DEVELOPMENTAL CELL

Amino Acid-Dependent mTORC1 Regulation by the Lysosomal Membrane Protein SLC38A9

(2015) Jennifer Jung et al.   MOLECULAR AND CELLULAR BIOLOGY

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1

(2015) Manuele Rebsamen et al.   NATURE

Gene essentiality and synthetic lethality in haploid human cells

(2015) V. A. Blomen et al.   SCIENCE

Lysosomal amino acid transporter SLC38A9 signals arginine sufficiency to mTORC1

(2015) S. Wang et al.   SCIENCE

Identification and characterization of essential genes in the human genome

(2015) T. Wang et al.   SCIENCE

Disruption of the Rag-Ragulator Complex by c17orf59 Inhibits mTORC1

(2015) Lawrence D. Schweitzer et al.   Cell Reports

The late endosomal p14–MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration

(2014) Natalia Schiefermeier et al.   JOURNAL OF CELL BIOLOGY

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

(2014) Chuan Yu et al.   PLoS One

Regulation of mTORC1 by amino acids

(2014) Liron Bar-Peled et al.   TRENDS IN CELL BIOLOGY

LAMTOR2 regulates dendritic cell homeostasis through FLT3-dependent mTOR signalling

(2014) Julia M. Scheffler et al.   Nature Communications

Stability of the Endosomal Scaffold Protein LAMTOR3 Depends on Heterodimer Assembly and Proteasomal Degradation

(2013) Mariana E. G. de Araújo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Improved low-resolution crystallographic refinement with Phenix and Rosetta

(2013) Frank DiMaio et al.   NATURE METHODS

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

The Protein Interaction Landscape of the Human CMGC Kinase Group

(2013) Markku Varjosalo et al.   Cell Reports

Low-resolution refinement tools inREFMAC5

(2012) Robert A. Nicholls et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1

(2012) Liron Bar-Peled et al.   CELL

Crystal Structure of the Gtr1pGTP-Gtr2pGDPProtein Complex Reveals Large Structural Rearrangements Triggered by GTP-to-GDP Conversion

(2012) Jae-Hee Jeong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The late endosomal adaptor p14 is a macrophage host-defense factor against Salmonella infection

(2012) N. Taub et al.   JOURNAL OF CELL SCIENCE

Ego3 Functions as a Homodimer to Mediate the Interaction between Gtr1-Gtr2 and Ego1 in the EGO Complex to Activate TORC1

(2012) Tianlong Zhang et al.   STRUCTURE

LAMTOR1 depletion induces p53-dependent apoptosis via aberrant lysosomal activation

(2012) M Malek et al.   Cell Death & Disease

The late endosome/lysosome-anchored p18-mTORC1 pathway controls terminal maturation of lysosomes

(2011) Yusuke Takahashi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Interactions between Kinase Scaffold MP1/p14 and Its Endosomal Anchoring Protein p18

(2011) James Magee et al.   BIOCHEMISTRY

Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation

(2011) R. Gong et al.   GENES & DEVELOPMENT

Robots, pipelines, polyproteins: Enabling multiprotein expression in prokaryotic and eukaryotic cells

(2011) Lakshmi Sumitra Vijayachandran et al.   JOURNAL OF STRUCTURAL BIOLOGY

StavroX—A Software for Analyzing Crosslinked Products in Protein Interaction Studies

(2011) Michael Götze et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Ragulator-Rag Complex Targets mTORC1 to the Lysosomal Surface and Is Necessary for Its Activation by Amino Acids

(2010) Yasemin Sancak et al.   CELL

The Crystal Structure of Dynein Intermediate Chain-Light Chain Roadblock Complex Gives New Insights into Dynein Assembly

(2010) Justin Hall et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Characterization of HBXIP: The Protein That Interacts with the Anti-Apoptotic Protein Survivin and the Oncogenic Viral Protein HBx

(2010) I. Garcia-Saez et al.   JOURNAL OF MOLECULAR BIOLOGY

MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments

(2010) José Gabadinho et al.   JOURNAL OF SYNCHROTRON RADIATION

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK–ERK pathway to late endosomes

(2009) Shigeyki Nada et al.   EMBO JOURNAL

Universal sample preparation method for proteome analysis

(2009) Jacek R Wiśniewski et al.   NATURE METHODS

CHAINSAW: a program for mutating pdb files used as templates in molecular replacement

(2008) Norman Stein   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7

(2008) Gerrit Langer et al.   Nature Protocols

The Rag GTPases Bind Raptor and Mediate Amino Acid Signaling to mTORC1

(2008) Y. Sancak et al.   SCIENCE