Article
Nanoscience & Nanotechnology
Wenhao Cheng, Masahiro Fukuda, Sundol Kim, Yuan Liu, Xingchi Chen, Christina Holmes, Yan Li, Hoyong Chung, Yi Ren, Jingjiao Guan
Summary: This study presents a robust engineering method for rupturing phagosomes based on a well-defined mechanism. The method uses microfabricated microparticles composed of uncrosslinked linear poly-(N-isopropylacrylamide) (PNIPAM) as phagocytic objects. By exposing the cells to a cold shock, the microparticle-containing phagosomes rupture. The osmotic pressure generated by dissolved microparticles is the probable cause of phagosomal rupture, as supported by modeling and experimental results.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Review
Immunology
Charneal L. Dixon, Katrina Mekhail, Gregory D. Fairn
Summary: Phagocytosis is a process used by cells to engulf particles, with proteins modified by lipids to regulate signal transduction and immune functions. S-acylation, specifically S-palmitoylation, is a reversible modification that plays a role in regulating phagocytosis and phagosome biology in macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Hailong Wang, Haiying Jiang, Xian Wu Cheng
Summary: This study identified CTSS as the main target molecule in the CTS family involved in atherosclerosis, mediating the disease progression by facilitating phagosome via macrophage. Through gene expression analysis and protein interaction network, CTSS was found to play a crucial role in atherosclerosis development.
Review
Cell Biology
Keren B. Turton, Rebecca J. Ingram, Miguel A. Valvano
Summary: Mutations in the CFTR gene disrupt chloride ion flux, affecting cellular homeostasis, particularly in respiratory epithelial cells where mucus accumulation occurs. Research indicates that CFTR mutations not only impact macrophage function, but also have direct effects on immune cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Editorial Material
Cell Biology
Jenny A. Nguyen, Catherine J. Greene, Robin M. Yates
Summary: Macrophages release partially digested immunostimulatory molecules extracellularly via a process called eructophagy, amplifying local inflammation.
Article
Pharmacology & Pharmacy
Chenpei Zhao, Huan Chen, Hao Liang, Xiaoyu Zhao, Wenli Tang, Maolian Wei, Youzhi Li, Jianlong Zhang, Xin Yu, Guozhong Chen, Hongwei Zhu, Linlin Jiang, Xingxiao Zhang
Summary: Probiotic Lactobacillus plantarum RS-09 has been found to modulate M1 macrophage polarization and induce TLR2-linked NF-kappa B signaling activity, enhancing the immune response against Salmonella Typhimurium infection. Additionally, RS-09 relieves splenomegaly, body weight loss, and death rate associated with the infection.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Li Tang, Bo Tang, Yuanyuan Lei, Min Yang, Sumin Wang, Shiping Hu, Zhuo Xie, Yaojiang Liu, Israel Vlodavsky, Shiming Yang
Summary: Chronic gastritis caused by H. pylori infection is associated with up-regulation of heparanase, which facilitates the colonization of H. pylori in the gastric mucosa and exacerbates gastritis. Heparanase sustains a vicious cycle of inflammation and tumor progression by activating macrophages and promoting cytokine release, potentially increasing the risk of gastric cancer. Inhibition of heparanase may be a potential therapeutic strategy for reducing the risk of gastritis and gastric cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Felix Breyer, Anetta Hartlova, Teresa Thurston, Helen R. Flynn, Probir Chakravarty, Julia Janzen, Julien Peltier, Tiaan Heunis, Ambrosius P. Snijders, Matthias Trost, Steven C. Ley
Summary: TPL-2 kinase mediates TLR activation of MAP kinases to modulate cytokine expression in myeloid cells, and also plays a crucial role in regulating phagosome maturation for efficient killing of phagocytosed microbes.
Article
Biochemistry & Molecular Biology
Seung-U Son, Sue Jung Lee, Kwang-Soon Shin
Summary: This study elucidated the intracellular signaling pathways involved in macrophage activation by REP-I polysaccharide purified from radish leaves. REP-I enhanced the expression and secretion of immune-related factors in macrophages. The effects were mediated by phosphorylation of MAPK and NF-κB pathways, with different pathways involved in the secretion of different factors. The study also revealed the involvement of pattern recognition receptors in the effects of REP-I.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Jinhui Shang, Qian Yang, Wenjun Fan, Yancao Chen, Decui Tang, Haowei Guo, Bin Xiong, Shuangyan Huang, Xiao-Bing Zhang
Summary: The multilayered Au@MnOx@SiO2 nanoparticle was developed as a robust pH-sensitive plasmonic nanosensor for real-time monitoring of dynamic acidification features during phagosome maturation process in macrophages. The color changes of nanoprobes indicated a slow-to-fast acidification translation over time, which was related to V-ATPase activation. This nanosensor shows promise for studying phagosome dysfunctions and related disease pathogenesis.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Haiqi He, Kenneth J. Genovese, Ryan J. Arsenault, Christina L. Swaggerty, Casey N. Johnson, J. Allen Byrd, Michael H. Kogut
Summary: This study found that Salmonella infection can alter host metabolism to increase energy and metabolites for intracellular replication. Infection reduced glycolysis and enhanced mitochondrial oxidative phosphorylation in chicken macrophages, while promoting M2 polarization. These results suggest that modulation of host cell metabolism and macrophage polarization contribute to the survival of S. enteritidis.
Review
Cell Biology
Johannes Westman, Sergio Grinstein
Summary: The ability of phagosomes to halt microbial growth is closely linked to their ability to acidify their luminal pH, but certain pathogens can survive and replicate inside phagosomes by targeting the pH-regulatory machinery of host cells to survive or escape.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Ankit Pandeya, Yan Zhang, Jian Cui, Ling Yang, Jeffery Li, Guoying Zhang, Congqing Wu, Zhenyu Li, Yinan Wei
Summary: Inflammasome activation is crucial in defending against bacterial infection. In this study, we discovered that inflammasome activation plays a critical role in the pathogenesis of Salmonella systemic infection. Deficiency of caspase-1 or gasdermin-D prolonged survival, reduced proinflammatory cytokine concentrations, and protected against coagulopathy during Salmonella infection. The activation of NAIP/NLRC4 inflammasome and SPI1 was found to trigger Salmonella-induced coagulopathy, while the caspase-11/NLRP3 pathway was also involved.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Developmental Biology
Fan Zeng, Julia Wunderer, Willi Salvenmoser, Michael W. Hess, Peter Ladurner, Ute Rothbaecher
DEVELOPMENTAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Julia Wunderer, Birgit Lengerer, Robert Pjeta, Philip Bertemes, Leopold Kremser, Herbert Lindner, Thomas Ederth, Michael W. Hess, David Stock, Willi Salvenmoser, Peter Ladurner
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Neurosciences
Barbara Hausott, Jong-Whi Park, Taras Valovka, Martin Offterdinger, Michael W. Hess, Stephan Geley, Lars Klimaschewski
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Cardiac & Cardiovascular Systems
Can Gollmann-Tepekoeylue, Leo Poelzl, Michael Graber, Jakob Hirsch, Felix Naegele, Daniela Lobenwein, Michael W. Hess, Michael J. Blumer, Elke Kirchmair, Johannes Zipperle, Carina Hromada, Severin Muehleder, Hubert Hackl, Martin Hermann, Hemse Al Khamisi, Martin Foerster, Michael Lichtenauer, Rainer Mittermayr, Patrick Paulus, Helga Fritsch, Nikolaos Bonaros, Rudolf Kirchmair, Joost P. G. Sluijter, Sean Davidson, Michael Grimm, Johannes Holfeld
CARDIOVASCULAR RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Giorgia Lamberti, Cedric H. De Smet, Mihaela Angelova, Leopold Kremser, Nicole Taub, Caroline Herrmann, Michael W. Hess, Johannes Rainer, Ivan Tancevski, Ruerdiger Schweigreiter, Reinhard Kofler, Thomas Schmiedinger, Ilja Vietor, Zlatko Trajanoski, Christer S. Ejsing, Herbert H. Lindner, Lukas A. Huber, Taras Stasyk
Article
Biology
Robert Pjeta, Julia Wunderer, Philip Bertemes, Teresa Hofer, Willi Salvenmoser, Birgit Lengerer, Stefan Coassin, Gertraud Erhart, Christian Beisel, Daniel Sobral, Leopold Kremser, Herbert Lindner, Marco Curini-Galletti, Claus-Peter Stelzer, Michael W. Hess, Peter Ladurner
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Review
Cell Biology
Mariana E. G. de Araujo, Gudrun Liebscher, Michael W. Hess, Lukas A. Huber
Article
Medicine, General & Internal
Michael W. Hess, Iris M. Krainer, Przemyslaw A. Filipek, Barbara Witting, Karin Gutleben, Ilja Vietor, Heinz Zoller, Denise Aldrian, Ekkehard Sturm, James R. Goldenring, Andreas R. Janecke, Thomas Mueller, Lukas A. Huber, Georg F. Vogel
Summary: The study thoroughly characterized the ultrastructural and immuno-cytochemical phenotype of hepatocytes and duodenal enterocytes from a unique case of an adult MYO5B-PFIC patient. Advanced methods were used in combination with standard procedures to reveal various abnormalities related to this rare disease.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Viktoria Zaderer, Wilfried Posch, Ronald Gstir, Przemyslaw A. Filipek, Guenther K. Bonn, Pornanong Aramwit, Lukas A. Huber, Doris Wilflingseder
Summary: The natural essence P80 has been shown to affect the function of DCs, with high concentrations leading to increased cell death and moderate concentrations showing effective antiviral effects. P80 natural essence significantly enhances DC maturation and co-stimulatory capacity through p38 MAPK phosphorylation, providing a defense against HIV-1 virus.
Article
Cell Biology
Cecilia Cattelani, Dominik Lesiak, Gudrun Liebscher, Isabel I. Singer, Taras Stasyk, Moritz H. Wallnoefer, Alexander M. Heberle, Corrado Corti, Michael W. Hess, Kristian Pfaller, Marcel Kwiatkowski, Peter P. Pramstaller, Andrew A. Hicks, Kathrin Thedieck, Thomas Muller, Lukas A. Huber, Mariana Eca Guimaraes de Araujo
Summary: The study reveals the role of SZT2 in the amino acid-sensing branch of mTORC1, with mutations in this gene causing severe neurodevelopmental and epileptic encephalopathy. Further analysis indicates enhanced mTORC1 signaling activation and higher levels of autophagic components in SZT2 ablated cells.
Article
Multidisciplinary Sciences
Zhicheng Cui, Gennaro Napolitano, Mariana E. G. de Araujo, Alessandra Esposito, Jlenia Monfregola, Lukas A. A. Huber, Andrea Ballabio, James H. H. Hurley
Summary: The transcription factor TFEB is phosphorylated by mTORC1, which is unique in its mechanism and dependent on the activation of FLCN6,7. The cryogenic-electron microscopy analysis reveals that two Rag-Ragulator complexes present each TFEB molecule to mTOR active site. The non-canonical Rag dimer binds the first helix of TFEB with a RagC(GDP)-dependent aspartate clamp and plays a crucial role in TFEB phosphorylation.
Article
Biology
Katharina M. C. Klee, Michael W. Hess, Michael Lohmueller, Sebastian Herzog, Kristian Pfaller, Thomas Mueller, Georg F. Vogel, Lukas A. Huber, Michel Bagnat
Summary: In this study, we conducted a genome-wide CRISPR/Cas9 screen combined with FACS to identify novel regulators of epithelial polarization and protein secretion in human polarized epithelial cells. Through high-resolution microscopy, we confirmed the role of TM9SF4, anoctamin 8, and ARHGAP33 in epithelial polarization and apical cargo secretion. This study provides a powerful tool for studying these processes and offers a dataset for the investigation of novel congenital diseases associated with epithelial polarization and polarized transport.
Article
Genetics & Heredity
Ferda O. Hosnut, Andreas R. Janecke, Gulseren Sahin, Georg F. Vogel, Naz G. Lafci, Paul Bichler, Thomas Mueller, Lukas A. Huber, Taras Valovka, Aysel U. Aksu
Summary: Congenital glucose-galactose malabsorption is a rare autosomal recessive disorder caused by mutations in SLC5A1 gene. Our study reports clinical and molecular data from 11 affected individuals in four unrelated Turkish families. Two novel SLC5A1 missense variants, p.Gly43Arg and p.Ala92Val, were identified in two families and linked to the disease. Our findings expand the mutational spectrum of this rare disorder.