Article
Cell & Tissue Engineering
Salma Alasmar, Jez Huang, Karishma Chopra, Ewa Baumann, Amy Aylsworth, Melissa Hewitt, Jagdeep K. Sandhu, Joseph S. Tauskela, Robert N. Ben, Anna Jezierski
Summary: This study investigated the role of ice recrystallization inhibitors (IRIs) as cryoprotectants for iPSCs and iPSC-derived neurons (iPSC-Ns). The results showed that IRIs, specifically 2FA, increased the post-thaw viability and recovery of iPSCs without affecting pluripotency. Although 2FA supplementation did not significantly improve iPSC-N cell post-thaw viability, 2FA cryopreserved iPSC-Ns exhibited better restoration of neuronal network activity and synaptic function compared to controls. This study highlights the importance of optimizing cryopreservation media formulations with IRIs to improve functional cryopreservation of iPSCs and post-mitotic iPSC-Ns.
Article
Neurosciences
Christian Schinke, Valeria Fernandez Vallone, Andranik Ivanov, Yangfan Peng, Peter Koertvelyessy, Luca Nolte, Petra Huehnchen, Dieter Beule, Harald Stachelscheid, Wolfgang Boehmerle, Matthias Endres
Summary: Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect with no predictive biomarkers or preventive treatments available. Using sensory neurons derived from induced pluripotent stem cells as an experimental model for CIPN, researchers found differential responses to neurotoxic drugs between cell lines from different healthy donors, showcasing potential for studying CIPN pathogenesis and neuroprotective treatments.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Li-Jie Hsu, Chao-Lin Liu, Ming-Ling Kuo, Chia-Ning Shen, Chia-Rui Shen
Summary: Cell therapy involves using cells selected, manipulated, and treated outside the body to treat or prevent human disease. iPSCs are ideal cell sources due to their unique characteristics. NK cells are effective tumor killers without causing GVHD, making them excellent candidates for cell therapy.
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Tae-Kyung Hong, Jae-Hoon Song, So-Been Lee, Jeong-Tae Do
Summary: Assisted reproductive technologies (ARTs) have limitations in rectifying germ cell aplasia such as non-obstructive azoospermia (NOA) and oocyte maturation failure syndrome. In vitro gametogenesis shows promise in overcoming infertility, with research focusing on deriving male and female germ cells from pluripotent stem cells (PSCs). This technique not only helps understand the biological mechanism of gamete development but also offers insights into its application in infertility treatments.
Article
Environmental Sciences
Miodrag Stojkovic, Francisco Manuel Ortuno Guzman, Dongjun Han, Petra Stojkovic, Joaquin Dopazo, Konstantina M. Stankovic
Summary: Plastic pollution is rapidly increasing, but its impact on human health is poorly understood. This study demonstrates the use of human cellular models to identify molecular biomarkers of environmental pollution and potentially decipher the origins of environmental diseases.
ENVIRONMENTAL POLLUTION
(2023)
Article
Cell Biology
T. Hartung, M. Rhein, N. Kalmbach, N. Thau-Habermann, M. Naujock, L. Mueschen, H. Frieling, J. Sterneckert, A. Hermann, F. Wegner, S. Petri
Summary: ALS is a rapidly progressive disease affecting motor neurons, and epigenetic modifications of gene expression have been identified as potential mechanisms. This study found significant differences in FUS gene expression and promotor methylation in mutant FUS motor neurons compared to healthy controls, suggesting that targeting disturbed methylation could potentially be a novel therapeutic strategy for ALS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jennifer Imm, Ehsan Pishva, Muhammadd Ali, Talitha L. Kerrigan, Aaron Jeffries, Joe Burrage, Enrico Glaab, Emma L. Cope, Kimberley M. Jones, Nicholas D. Allen, Katie Lunnon
Summary: This study investigated dynamic changes in DNA methylation during neuronal differentiation using induced pluripotent stem cell-based models. Significant loci with progressive alterations in DNA methylation were identified during the differentiation process. A network analysis revealed a set of densely connected genes, with STAT3 showing the highest connectivity and influencing neuronal differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Peter Chovanec, Amanda J. Collier, Christel Krueger, Csilla Varnai, Claudia I. Semprich, Stefan Schoenfelder, Anne E. Corcoran, Peter J. Rugg-Gunn
Summary: The authors generated a high-resolution atlas of gene regulatory interactions, chromatin profiles, and transcription factor occupancy in naive and primed human pluripotent stem cells. The study provides insights into the mechanisms underlying the transition between naive and primed pluripotency states.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Maki Takeda, Emiko Ito, Kazumasa Minami, Akima Harada, Noriko Mochizuki-Oda, Yoshiki Sawa, Shigeru Miyagawa
Summary: Irradiation of hiPSC-CMs can reduce the number of undifferentiated hiPSCs and tumor formation risk, without affecting the growth and morphology of cardiomyocytes. Tumorigenicity tests in mice showed lower tumor formation frequency in the irradiation group compared to the control group.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Fatma Dogan, Rakad M. Kh Aljumaily, Mark Kitchen, Nicholas R. Forsyth
Summary: Pluripotent stem cells are sensitive to oxygen concentration, and a low oxygen environment can enhance their functional profile and epigenetic regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xiangyu Wang, Mingju Sun, Jingyu Li, Xuexiong Song, Hongbin He, Yanjun Huan
Summary: Exogenous factor EB1 was found to have detrimental effects on porcine early embryos, while melatonin showed effectiveness in rescuing the defects caused by EB1. This study provides new insights into enhancing embryo quality and promoting human and animal reproduction.
Review
Cell Biology
Mourad A. M. Aboul-Soud, Alhusain J. Alzahrani, Amer Mahmoud
Summary: The discovery of induced pluripotent stem cells (iPSCs) has revolutionized biological research, providing valuable models for drug screening and disease modeling in regenerative medicine. iPSCs have the potential to circumvent ethical concerns surrounding embryonic stem cells and offer a promising avenue for clinical translation research.
Review
Cell Biology
Benjamin Theisen, Austin Holtz, Viswanathan Rajagopalan
Summary: Brugada Syndrome, a cardiac channelopathy, is associated with gene mutations and noncoding RNAs, leading to life-threatening arrhythmias. Studies have shown that the majority of loci associated with electrophysiological traits are located in noncoding regulatory regions, affecting gene expression dosage and cardiac ion channel function.
Article
Cell & Tissue Engineering
Marco Schmidt, Kira Zeevaert, Mohamed H. Elsafi Mabrouk, Roman Goetzke, Wolfgang Wagner
Summary: Quality control of induced pluripotent stem cells is challenging, and determining trilineage differentiation potential is crucial for validating their pluripotent state. In this study, GermLayerTracker, a combination of site-specific DNA methylation assays, was developed as a biomarker for early germ layer specification. Specific CG dinucleotides (CpGs) with characteristic DNA methylation patterns were identified in the pluripotent state and during differentiation into endoderm, mesoderm, and ectoderm. The GermLayerTracker can be used for quality control of pluripotent cells and to estimate lineage-specific commitment during initial differentiation events.
Article
Obstetrics & Gynecology
Laila Noli, Caroline Ogilvie, Yacoub Khalaf, Dusko Ilic
HUMAN REPRODUCTION UPDATE
(2017)
Editorial Material
Cell & Tissue Engineering
Dusko Ilic, Oliver Ball
REGENERATIVE MEDICINE
(2016)
Editorial Material
Cell & Tissue Engineering
Dusko Ilic
REGENERATIVE MEDICINE
(2016)
Editorial Material
Cell & Tissue Engineering
Cristian Miere, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
Liani Devito, Laureen Jacquet, Anastasia Petrova, Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Editorial Material
Cell & Tissue Engineering
Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
STEM CELL RESEARCH
(2016)
Review
Cell & Tissue Engineering
Dusko Ilic, Caroline Ogilvie
Article
Multidisciplinary Sciences
Marta N. Shahbazi, Antonio Scialdone, Natalia Skorupska, Antonia Weberling, Gaelle Recher, Meng Zhu, Agnieszka Jedrusik, Liani G. Devito, Laila Noli, Iain C. Macaulay, Christa Buecker, Yakoub Khalaf, Dusko Ilic, Thierry Voet, John C. Marioni, Magdalena Zernicka-Goetz
Article
Multidisciplinary Sciences
Florian T. Merkle, Sulagna Ghosh, Nolan Kamitaki, Jana Mitchell, Yishai Avior, Curtis Mello, Seva Kashin, Shila Mekhoubad, Dusko Ilic, Maura Charlton, Genevieve Saphier, Robert E. Handsaker, Giulio Genovese, Shiran Bar, Nissim Benvenisty, Steven A. McCarroll, Kevin Eggan
Article
Cell & Tissue Engineering
Dusko Ilic, Sharon Yau
REGENERATIVE MEDICINE
(2017)
Article
Multidisciplinary Sciences
Heba Badraiq, Aleksandra Cvoro, Antonio Galleu, Marisa Simon, Cristian Miere, Carl Hobbs, Reiner Schulz, Richard Siow, Francesco Dazzi, Dusko Ilic
SCIENTIFIC REPORTS
(2017)
