Article
Plant Sciences
Yushan Zhai, Quan Yuan, Shiyou Qiu, Saisai Li, Miaomiao Li, Hongying Zheng, Guanwei Wu, Yuwen Lu, Jiejun Peng, Shaofei Rao, Jianping Chen, Fei Yan
Summary: Chloroplast NADH dehydrogenase-like complex M subunit gene (NdhM) plays a crucial role in plant defense against viral infection by regulating chloroplast clustering to resist virus invasion.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Kunxin Wu, Yan Fu, Yanli Ren, Linyu Liu, Xiuchun Zhang, Mengbin Ruan
Summary: This study reveals that Turnip crinkle virus (TCV) activates mRNA decay pathways during infection, and the viral protein interferes with this pathway to facilitate viral replication.
Article
Biochemistry & Molecular Biology
Zhangli Zuo, Milena Roux, Eleazar Rodriguez, Morten Petersen
Summary: The mRNA decapping components LSM1, PAT1, PATH1, and PATH2 are essential for the infection of turnip mosaic virus (TuMV), and TuMV hijacks decapping components via PAT proteins to support viral infection.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Microbiology
Esther O. Bello, Yingshuai Yang, Yue Fang, Mengzhu Chai, Xue Jiang, Yameng Luan, Yuting Wang, Yating Guo, Xiao-Yun Wu, Xiaofei Cheng, Xiao-Xia Wu
Summary: The P1 protein in Potyvirus is crucial for viral infection and adaptation, although its exact mechanism is not fully understood. Through yeast-two-hybrid screening, eight potential P1-interacting proteins in Arabidopsis were identified, with NODULIN 19 (NOD19) being selected for further investigation. Further experiments confirmed the interaction between TuMV P1 and NOD19, which is a membrane-associated protein expressed mainly in plant aerial parts. Knockdown of NOD19 in Arabidopsis and soybean resulted in attenuated infection of TuMV and soybean mosaic virus, respectively. These findings suggest that NOD19 is a host factor required for robust viral infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Ying Zhai, Anirban Roy, Hao Peng, Daniel L. Mullendore, Gurpreet Kaur, Bikash Mandal, Sunil Kumar Mukherjee, Hanu R. Pappu
Summary: This study identifies four silencing suppressors encoded by Croton yellow vein mosaic virus (CYVMV) and its cognate betasatellite, and investigates their subcellular localizations, interaction behavior, and roles in symptom induction and intercellular virus movement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Md Emran Ali, Sumyya Waliullah
Summary: Contrary to popular belief, the 2X35S promoter actually delayed the initiation of turnip crinkle virus (TCV) infection compared to the Core35S promoter. This suggests that the Core35S (or mini35S) promoter may be a better choice for generating infectious cDNA clones of TCV, as the stronger 2X35S promoter may lead to higher accumulation of a TCV protein that inhibits virus replication.
Article
Plant Sciences
Guanwei Wu, Zhaoxing Jia, Penghuan Rui, Hongying Zheng, Yuwen Lu, Lin Lin, Jiejun Peng, Shaofei Rao, Aiming Wang, Jianping Chen, Fei Yan
Summary: This study identified the importance of acidic dileucine motifs in the CI protein of Turnip mosaic virus for interaction with AP2 beta, which is crucial for efficient viral replication.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Linyu Liu, Haiyan Wang, Yan Fu, Wen Tang, Pingjuan Zhao, Yanli Ren, Zhixin Liu, Kunxin Wu, Xiuchun Zhang
Summary: The capsid protein of Turnip crinkle virus interacts with SGS3, enhancing viral accumulation and suppressing RNA silencing-mediated antiviral defense.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Mingzhen Zhang, Pan Gong, Linhao Ge, Zhaoyang Chang, Xiaofei Cheng, Xueping Zhou, Aiming Wang, Fangfang Li
Summary: The study reveals that XPO1 plays a crucial role in TuMV infection by interacting with NIb to facilitate its translocation to viral replication complexes, as well as mediating the nuclear export of sumoylated NIb and host factors tagged with SUMO3 to suppress plant immunity. Deficiencies of XPO1 in Arabidopsis and Nicotiana benthamiana plants inhibit TuMV replication and infection, highlighting the importance of XPO1 as a host factor in potyvirus infections.
Article
Microbiology
Mingzhen Zhang, Pan Gong, Linhao Ge, Yinzi Li, Zhaoyang Chang, Rui Qiao, Xueping Zhou, Aiming Wang, Fangfang Li
Summary: The nuclear localization signal (NLS) and nuclear export signal (NES) play important roles in controlling the import and export of proteins. Mutations in the NLS and NES of the NIb protein of turnip mosaic virus (TuMV) can inhibit viral RNA accumulation and systemic infection. We found that XPO1 interacts with the functional NLS and NES of NIb to regulate its nucleocytoplasmic transport and promote viral infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Lang Qin, Shiwen Ding, Zhilei Wang, Runzhou Jiang, Zhen He
Summary: This study analyzed the compositional bias and codon usage of TuMV and found that there were changes in the genomic composition and codon usage choice. Statistical analysis showed that natural selection was the main factor affecting the codon usage patterns of TuMV. Additionally, the study revealed that TuMV genes were strongly adapted to Brassica oleracea and potentially preferred this host.
Article
Virology
Kaida Ding, Zhaoxing Jia, Penghuan Rui, Xinxin Fang, Hongying Zheng, Jianping Chen, Fei Yan, Guanwei Wu
Summary: The study investigates the role of host factors and cellular protein changes in the early stages of Turnip mosaic virus (TuMV) infection in Nicotiana benthamiana plants. The researchers identified 225 differentially accumulated proteins (DAPs), including four upregulated proteins belonging to the UGT family, that influenced TuMV replication and reactive oxygen species production. This comparative proteomics analysis provides new insights into the mechanisms of plant viral infection.
Article
Biotechnology & Applied Microbiology
F. H. Tan, J. C. Kong, J. F. Ng, N. B. Alitheen, C. L. Wong, C. Y. Yong, K. W. Lee
Summary: The study successfully displayed a 10-residue peptide on the surface of TYMVcHis(6) VLPs, showing high stability under different pH and temperature conditions, and the ability to reassemble into VLPs after chemical denaturation.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Yaqin Wang, Hui Liu, Zhanqi Wang, Yushuang Guo, Tao Hu, Xueping Zhou
Summary: This study found that the P25 and P37 proteins of FLRaV play important roles in overcoming host RNA silencing and aggravating the symptoms and titer of virus infection, providing key insights into the molecular mechanisms underlying FLRaV infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Otulak-Koziel, Edmund Koziel, Edit Horvath, Jolan Csiszar
Summary: This study reveals the importance of the redox state of glutathione in the response of plants to virus inoculation. The transcriptionally regulated GSTU19 and GSTU24 play roles in the susceptible and resistance-like reaction of plants against TuMV, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Biochemistry & Molecular Biology
Martina Wirth, Stephane Mouilleron, Wenxin Zhang, Eva Sjottem, Yakubu Princely Abudu, Ashish Jain, Hallvard Lauritz Olsvik, Jack-Ansgar Bruun, Minoo Razi, Harold B. J. Jefferies, Rebecca Lee, Dhira Joshi, Nicola O'Reilly, Terje Johansen, Sharon A. Tooze
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jia Xuan Leong, Margot Raffeiner, Daniela Spinti, Gautier Langin, Mirita Franz-Wachtel, Andrew R. Guzman, Jung-Gun Kim, Pooja Pandey, Alyona E. Minina, Boris Macek, Anders Hafren, Tolga O. Bozkurt, Mary Beth Mudgett, Frederik Boernke, Daniel Hofius, Suayib Uestuen
Summary: Autophagy plays a crucial role in plant-bacteria interactions, where antimicrobial autophagy degrades intracellular pathogens and reveals a pathogen strategy of escaping elimination by hijacking the autophagy mechanism.
Review
Biochemistry & Molecular Biology
Paul Gouguet, Suayib Uestuen
Summary: Autophagy plays a crucial role in plant cellular homeostasis by recycling cellular components. Recent studies have revealed the interconnection between autophagy and canonical membrane trafficking pathways, leading to a better understanding of the inner workings of both pathways in plant cells.
Article
Biochemistry & Molecular Biology
Gesa Hoffmann, Amir Mahboubi, Heinrich Bente, Damien Garcia, Johannes Hanson, Anders Hafren
Summary: Viral infections cause significant stress on cellular RNA and trigger RNA surveillance pathways. In this study, PB components in Arabidopsis were found to be co-opted by the Cauliflower mosaic virus (CaMV) to facilitate viral replication and translation. This pro-viral role was not associated with RNA decay but instead helped in viral RNA translation. Dysfunctions in PB components exposed the virus to RNA silencing, similar to what has been observed in transgenes. Our study highlights the importance of PB components in maintaining efficient virus translation and their unique dependence on RNA silencing.
Article
Biochemistry & Molecular Biology
Margot Raffeiner, Suayib Uestuen, Tiziana Guerra, Daniela Spinti, Maria Fitzner, Sophia Sonnewald, Susanne Baldermann, Frederik Boernke
Summary: Xanthomonas effector protein XopS enhances disease susceptibility by repressing defense gene expression and suppressing stomatal immunity through interaction with and inhibition of WRKY40, a transcriptional regulator. XopS manipulation of WRKY40 stability alters downstream gene expression and phytohormone crosstalk to promote pathogen proliferation.
Review
Biochemistry & Molecular Biology
Jia Xuan Leong, Gautier Langin, Suayib Uestuen
Summary: Selective autophagy plays a crucial role in regulating cellular homeostasis and immune defense in plants. Although several selective autophagy receptors have been identified, little is known about those involved in plant immunity. We propose an in silico approach to identify novel receptors and investigate their regulatory roles in immune response using transcriptomic data from public databases. This study highlights the importance of selective autophagy in plant immunity and introduces new perspectives for research and applications.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Juncal Garcia-Garcia, Anne Kristin McLaren Berge, Katrine Stange Overa, Kenneth Bowitz Larsen, Zambarlal Bhujabal, Andreas Brech, Yakubu Princely Abudu, Trond Lamark, Terje Johansen, Eva Sjottem
Summary: TRIM27 is a multifunctional protein involved in various cellular functions. It has been found to facilitate mitochondrial clustering via SQSTM1/p62 and promote mitophagy by stabilizing phosphorylated TBK1 on mitochondria.
Review
Cell Biology
Nikoline Lander Rasmussen, Athanasios Kournoutis, Trond Lamark, Terje Johansen
Summary: NBR1 is an important autophagy receptor, serving as the main receptor in plants and co-existing with p62 in most animals. It participates in the selective autophagy of protein aggregates, and its mechanism and substrates are under investigation. Its roles in human diseases have also attracted attention.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Alexis Lebecq, Mehdi Doumane, Aurelie Fangain, Vincent Bayle, Jia Xuan Leong, Frederique Rozier, Maria Del Marques-Bueno, Laia Armengot, Romain Boisseau, Mathilde Laetitia Simon, Mirita Franz-Wachtel, Boris Macek, Suayib Ustun, Yvon Jaillais, Marie-Cecile Caillaud
Summary: Membrane lipids, especially phosphoinositides, are differentially enriched within the eukaryotic endomembrane system. In this study, the authors characterized the functional role of a phosphoinositide phosphatase, SAC9, in Arabidopsis. They found that the depletion of SAC9 led to misplaced accumulation of PI(4,5)P-2 on cortical intracellular compartments, impacting endocytic trafficking.
Editorial Material
Cell Biology
Nikoline Lander Rasmussen, Jianwen Zhou, Hallvard Olsvik, Stephanie Kaeser-Pebernard, Trond Lamark, Joern Dengjel, Terje Johansen
Summary: TNIP1 is degraded through autophagy after infection to allow expression of inflammatory genes and proteins, and its levels rise again later to counteract sustained inflammatory signaling. The phosphorylation of a TNIP1 LIR motif by TBK1 regulates its selective autophagy.
Editorial Material
Cell Biology
Athanasios Kournoutis, Terje Johansen
Summary: The Atg8 family of autophagy proteins is found to have roles in both the cytoplasm and the cell nucleus. In a recent study, Jimenez-Moreno et al. (2023. J. Cell Biol.) discovered that nuclear LC3B interacts with the LIM homeodomain transcription factor LMX1B, serving as a cofactor for LMX1B-mediated transcription of autophagy genes. This interaction provides stress protection and ensures the survival of midbrain dopaminergic neurons.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Plant Sciences
Gesa Hoffmann, Aayushi Shukla, Silvia Lopez-Gonzalez, Anders Hafren
Summary: Natural variation in the Arabidopsis thaliana/cauliflower mosaic virus pathosystem reveals diverse disease outcomes and identifies the ABA biosynthesis gene NCED9 as a novel susceptibility factor. This study is significant for understanding viral pathogenesis and ensuring food production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Gesa Hoffmann, Silvia Lopez-Gonzalez, Amir Mahboubi, Johannes Hanson, Anders Hafren
Summary: The Cauliflower mosaic virus can suppress stress granule assembly in plants by intersecting with components of the stress granule pathway. The virus forms condensates called viral factories, which consist of RNA and protein. The viral protein P6 plays a central role in these condensates, while host proteins show dynamic mobility within them. The study also found that components of stress granules localize to viral factories during infection, and the viral protein P6 can localize to stress granules and reduce their assembly.
Editorial Material
Biochemistry & Molecular Biology
Hallvard Lauritz Olsvik, Terje Johansen
Article
Multidisciplinary Sciences
Viola Nahse, Camilla Raiborg, Kia Wee Tan, Sissel Mork, Maria Lyngaas Torgersen, Eva Maria Wenzel, Mireia Nager, Veijo T. T. Salo, Terje Johansen, Elina Ikonen, Kay Oliver Schink, Harald Stenmark
Summary: DFCP1 is an ATPase located on omegasomes and plays a crucial role in selective autophagy by promoting the constriction of large omegasomes. It is essential for maintaining cellular homeostasis by removing damaged organelles and protein aggregates.
NATURE COMMUNICATIONS
(2023)