Article
Agriculture, Dairy & Animal Science
Yang Liu, Xiaomin Wang, Xiaoping Luo, Rui Wang, Bintao Zhai, Penglong Wang, Junyan Li, Xiaoye Yang
Summary: In this study, transcriptomic and proteomic differences in ivermectin-resistant and -susceptible strains of H. contortus were examined before and after Ivermectin treatment. The results showed that genes such as UDP-glycosyltransferases (UGT), glutathione S-transferase (GST), cytochrome P450 (CYP), and p-glycoprotein (Pgp) play important roles in H. contortus drug resistance. The findings provide useful information for understanding the resistance response of H. contortus.
Article
Agriculture, Dairy & Animal Science
Waresi Tuersong, Xin Liu, Yifan Wang, Simin Wu, Peixi Qin, Shengnang Zhu, Feng Liu, Chunqun Wang, Min Hu
Summary: This study explored the metabolome of an IVM-resistant H. contortus strain and analyzed the role of metabolism in IVM resistance. The results showed that amino acid metabolism may be mediated by the uptake of IVM and related to IVM resistance in H. contortus. This study contributes to our understanding of the mechanisms of IVM resistance and may provide effective approaches to manage infection by resistant strains of H. contortus.
Article
Parasitology
Paulius Baltrusis, Stephen R. Doyle, Peter Halvarsson, Johan Hoglund
Summary: The study reveals that in a field population from Sweden, despite an 89% reduction in parasites after ivermectin treatment, the surviving population remained highly genetically similar to the population before treatment, indicating that resistance likely evolved over time and is present on diverse haplotypes. The data provide new insights into the genome-wide effects of ivermectin selection in a field population and confirm the role of a previously identified quantitative trait locus in the development of resistance to ivermectin.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2022)
Article
Parasitology
Waresi Tuersong, Caixian Zhou, Simin Wu, Peixi Qin, Chunqun Wang, Wenda Di, Lu Liu, Hui Liu, Min Hu
Summary: This study identified differentially expressed genes (DEGs) in Ivermectin (IVM)-resistant Haemonchus contortus compared to susceptible strains, providing insights into the molecular mechanisms of IVM resistance. The study revealed potential new target genes related to lipid metabolism, cuticle structure, and important pathways such as antigen processing, lysosome, autophagy, apoptosis, and NOD1-like receptor signaling pathways. These findings contribute to a better understanding of IVM resistance in H. contortus.
PARASITES & VECTORS
(2022)
Article
Evolutionary Biology
Marielle Babineau, Eliza Collis, Angela Ruffell, Rowan Bunch, Jody McNally, Russell E. Lyons, Andrew C. Kotze, Peter W. Hunt
Summary: This study presents a new method for monitoring the ovine nematode parasite Haemonchus contortus using a SNP selection approach. The method can be used as a management assessment tool to identify drug-treatment failure, population incursions, and inbreeding signatures. It demonstrates high stability and accuracy and can be applied to other parasite species.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Multidisciplinary Sciences
Christina D. Moon, Luis Carvalho, Michelle R. Kirk, Alan F. McCulloch, Sandra Kittelmann, Wayne Young, Peter H. Janssen, Dave M. Leathwick
Summary: Anthelmintic treatments, including a controlled release capsule (CRC) and a long-acting moxidectin injection (LAI), were found to significantly impact the rumen microbiota composition in adult ewes. The CRC treatment increased the relative abundances of specific archaeal species, while the LAI treatment led to minor changes in the bacterial and fungal communities. Overall, the anthelmintic treatments resulted in few but highly significant changes to the rumen microbiota composition.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Sasha Hayes, Aya C. Taki, Kah Yean Lum, Joseph J. Byrne, Jonathan M. White, Merrick G. Ekins, Robin B. Gasser, Rohan A. Davis
Summary: This study identified a marine extract derived from an Australian sponge with activity against an economically important parasitic nematode. Further analysis led to the purification of four known sesterterpenes and the revision of their chemical structures. The findings suggest that these sesterterpenes deserve further investigation for their activity against nematodes.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Agriculture, Dairy & Animal Science
Xiaoping Luo, Shuyi Wang, Ying Feng, Penglong Wang, Gaowa Gong, Tianlong Guo, Xingang Feng, Xiaoye Yang, Junyan Li
Summary: Haemonchus contortus, a parasite affecting sheep, is becoming resistant to ivermectin due to the widespread and uncontrolled use of the drug. P-glycoprotein (P-gp) is involved in the resistance mechanism, and changes in P-gp expression can be used as a marker for ivermectin resistance in H. contortus. This study analyzes the effects of ivermectin on P-gp expression in H. contortus larvae and confirms that P-gp expression varies among different strains and geographic environments.
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Environmental Sciences
Yu Li, Jianhua Chen, Guangpeng Feng, Qingyun Wang, Rulong Xia, Chao Song, Haihua Wang, Yanping Zhang
Summary: In this study, the genetic diversity of Coilia nasus was investigated using 2b-RAD simplified genome sequencing technology. The results showed that the genetic purity of the populations was low and needs to be improved. The populations exhibited high genetic diversity, indicating strong adaptability and potential for distribution and resource growth. Moderate genetic differentiation was observed among the populations, suggesting a common spawning ground for these migratory species and the need to protect the gene purity of C. nasus.
Article
Public, Environmental & Occupational Health
Valeria Nayara Gomes Mendes De Oliveira, Luciana Werneck Zuccherato, Talita Rodrigues Dos Santos, Elida Mara Leite Rabelo, Luis Fernando Viana Furtado
Summary: Preventive chemotherapy is the main strategy recommended by WHO to control nematode infections in humans, but drug resistance in worm populations is a concern. This study identified a mutation associated with benzimidazole resistance in Trichuris trichiura populations in Brazil, highlighting the need for regular monitoring of drug resistance-related single-nucleotide polymorphisms.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Multidisciplinary Sciences
Jeba R. J. Jesudoss Chelladurai, Katy A. Martin, Pam Vardaxis, Craig Reinemeyer, Paramasivan Vijayapalani, Alan P. Robertson, Matthew T. Brewer
Summary: In this study, the researchers investigated the role of permeability glycoproteins (P-gps) in Toxocara canis drug tolerance. They found that T. canis has 13 annotated P-gp genes, with at least 10 expressed in adults and hatched larvae, and at least 8 expressed in somatic larvae. However, treatment with macrocyclic lactones did not significantly increase P-gp expression. Further studies are needed to understand the contribution of P-gps to macrocyclic lactone tolerance in T. canis.
SCIENTIFIC REPORTS
(2023)
Article
Parasitology
Ekta Singh, Dinesh Chandra, Arvind Prasad, Navneet Kaur
Summary: The study was conducted in small ruminant farms in the western region of Uttar Pradesh, India. Two in vitro tests were performed to assess ivermectin resistance in field isolates of H. contortus, showing the susceptibility of the isolates in the area under study. The results of the two tests had statistically non-significant standard deviations, concluding that both in vitro tests are reliable for detecting resistance against different anthelmintics including ivermectin.
ACTA PARASITOLOGICA
(2021)
Article
Microbiology
David Emanuel Reyes-Guerrero, Veronica Jimenez-Jacinto, Rogelio Alejandro Alonso-Morales, Miguel Angel Alonso-Diaz, Jocelyn Maza-Lopez, Rene Camas-Pereyra, Agustin Olmedo-Juarez, Rosa Isabel Higuera-Piedrahita, Maria Eugenia Lopez-Arellano
Summary: In this study, the transcriptome of Hc was assembled and annotated to investigate the differential gene expression between two Mexican Hc strains with different anthelmintic resistance statuses. The study identified differentially expressed genes and enriched gene ontology terms related to anthelmintic resistance and nematode biology.
Article
Parasitology
Zaira M. Estrada-Reyes, D. Owen Rae, Raluca G. Mateescu
Summary: Florida Native sheep is an ancient breed well-adapted to humid and hot climates, with superior ability to regulate nematode burdens. Both additive and non-additive genetic effects play important roles in resistance to natural Haemonchus contortus infections in Florida Native sheep, with potential genetic markers identified.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)