Article
Multidisciplinary Sciences
Marie Pariollaud, Lara H. Ibrahim, Emanuel Irizarry, Rebecca M. Mello, Alanna B. Chan, Brian J. Altman, Reuben J. Shaw, Michael J. Bollong, R. Luke Wiseman, Katja A. Lamia
Summary: Chronic circadian disruption increases tumor burden in a mouse model of lung cancer through enhanced expression and nuclear accumulation of HSF1.
Article
Cell Biology
Mooud Amirkavei, Flavia Plastino, Anders Kvanta, Kai Kaarniranta, Helder Andre, Ari Koskelainen
Summary: Cells have stress-response pathways to cope with stress factors for homeostasis maintenance; low doses of stress may be beneficial; HHS was found to enhance autophagy gene expression and activation through HSF1.
Review
Medicine, Research & Experimental
Yeh Chin, Khanisyah E. Gumilar, Xing-Guo Li, Brahmana A. Tjokroprawiro, Chien-Hsing Lu, Jianrong Lu, Ming Zhou, Robert W. Sobol, Ming Tan
Summary: HSF1 is a master regulator of heat shock responsive signaling and also regulates a non-heat shock responsive transcriptional network. It plays important roles in cellular transformation and cancer development. Research on HSF1 has been active due to its critical functions in handling stressful cellular states. New functions and molecular mechanisms have been continuously discovered, providing new targets for cancer treatment strategies. This article reviews the essential roles and mechanisms of HSF1 in cancer cells, focusing on recently discovered functions and their underlying mechanisms, as well as advances in HSF1 inhibitors for cancer drug development.
Article
Biochemistry & Molecular Biology
Pratibha Srivastava, Ryosuke Takii, Mariko Okada, Mitsuaki Fujimoto, Akira Nakai
Summary: The CKM module plays a crucial role in heat-shock transcription, promoting HSP70 expression through the kinase activity of CDK8 and CDK19, and maintaining proteostasis capacity to protect cells against proteotoxic stress.
Article
Oncology
Natalia Vydra, Agnieszka Toma-Jonik, Patryk Janus, Katarzyna Mrowiec, Tomasz Stokowy, Magdalena Glowala-Kosinska, Damian Robert Sojka, Magdalena Olbryt, Wieslawa Widlak
Summary: Epithelial-to-mesenchymal transition (EMT) is important for development and wound healing, but can also contribute to organ fibrosis and cancer metastasis. This study suggests that the heat shock transcription factor 1 (HSF1) may be involved in EMT in human mammary epithelial cells, and inhibiting HSF1 could potentially be a treatment for breast cancer.
Article
Multidisciplinary Sciences
Roger S. Smith, Seesha R. Takagishi, David R. Amici, Kyle Metz, Sitaram Gayatri, Milad J. Alasady, Yaqi Wu, Sonia Brockway, Stephanie L. Taiberg, Natalia Khalatyan, Mikko Taipale, Sandro Santagata, Luke Whitesell, Susan Lindquist, Jeffrey N. Savas, Marc L. Mendillo
Summary: Heat shock factor 1 (HSF1) plays a crucial role in the heat shock response and tumorigenesis. This study reveals that HSF2 interacts with HSF1 in various types of cancer. HSF1 and HSF2 have similar chromatin occupancy and regulate a common set of genes, including HSPs and noncanonical transcriptional targets critical for supporting malignancy.
Review
Plant Sciences
Yunzhuan Zhou, Fuxiang Xu, Yanan Shao, Junna He
Summary: As global warming intensifies, the earth's average temperature continues to rise. Plants have developed two main mechanisms, the heat stress response and the thermomorphogenesis response, to cope with elevated temperatures. This review summarizes recent progress in understanding these heat-responsive molecular regulatory pathways in plants and discusses future perspectives in this field.
Article
Medicine, Research & Experimental
Mingyuan Wang, Jiang Zou, Jinjin Wang, Meidong Liu, Ke Liu, Nian Wang, Kangkai Wang
Summary: This study investigated the protective effect of metformin on myocardial tissue and identified HSF1 as a key target. Metformin stimulated the activation of HSF1, which up-regulated AMPKa2 and activated the downstream AMPK/mTOR signaling pathway. HSF1 exhibited a higher affinity for metformin than AMPK, suggesting that it may be a more sensitive target.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Thomas MacVicar, Thomas Langer
Summary: Mechanical forces influence extracellular matrix stiffness, which in turn alters mitochondrial shape and function, enhancing resistance to oxidative stress and promoting cell survival.
Article
Biochemistry & Molecular Biology
Eileen T. Burchfiel, Anniina Vihervaara, Michael J. Guertin, Rocio Gomez-Pastor, Dennis J. Thiele
Summary: Studies have identified changes in HSF1 interacting proteins under normal growth and various stress conditions, as well as new HSF1 interacting proteins in a Huntington's disease cell model, showing involvement in cellular functions such as DNA repair and mRNA processing.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tamara Isermann, Ozge Cicek Sener, Adrian Stender, Luisa Klemke, Nadine Winkler, Albrecht Neesse, Jinyu Li, Florian Wegwitz, Ute M. Moll, Ramona Schulz-Heddergott
Summary: The discovery of the WT P53-HSF1 axis in cancer reveals a mechanism driving p53LOH, where the WT p53 allele suppresses HSF1, preventing mutant p53 stabilization and gain-of-function activities, leading to selective pressure for p53LOH.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Aysenur Akkulak, Emre Yesiloren, Abdullah Yalcin, Gizem Donmez Yalcin
Summary: Heat shock response (HSR) regulated by heat shock factor 1 (HSF1) is the most important mechanism to prevent protein aggregation in neurodegenerative diseases. Excitotoxicity, characterized by excess glutamate accumulation, is observed in various neurodegenerative diseases and acute brain injuries. This study investigates the molecular mechanism linking excitotoxicity and HSR and suggests that heat shock preconditioning can protect against excitotoxicity-related cell death and degeneration.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mariko Okada, Mitsuaki Fujimoto, Pratibha Srivastava, Akanksha Pandey, Ryosuke Takii, Akira Nakai
Summary: Upon heat shock, HSF1 binds to HSEs in the promoters of HSP-encoding genes and recruits Mediator. The transcriptional regulators may be concentrated in phase-separated condensates around the promoters. Using an experimental system, it was demonstrated that MED12 is concentrated in HSF1 condensates and plays an important role in their formation.
Article
Oncology
Weikun Qian, Ke Chen, Tao Qin, Ying Xiao, Jie Li, Yangyang Yue, Cancan Zhou, Jiguang Ma, Wanxing Duan, Jianjun Lei, Liang Han, Li Li, Xin Shen, Zheng Wu, Qingyong Ma, Zheng Wang
Summary: In this study, pharmacological inhibition of HSF1 was found to slow pancreatic cancer initiation and suppress pancreatitis-induced formation of pancreatic precancerous lesions. Bioinformatics analysis revealed a close link between HSF1 and the EGFR pathway, with their parallel activation in pancreatic precancerous lesions. Additionally, inhibition of EGFR was shown to suppress pancreatic cancer initiation and HSF1 activation in vivo, indicating a partly HSF1-dependent mechanism of EGFR-mediated pancreatic cancer tumorigenesis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Plant Sciences
Abdulhakim A. Aldubai, Abdullah A. Alsadon, Hussein H. Migdadi, Salem S. Alghamdi, Sulieman A. Al-Faifi, Muhammad Afzal
Summary: This study aimed to determine the response of tomato to heat stress under different temperature conditions and characterized the heat shock genes in tomato genotypes. The results showed that heat shock factor genes play an important role in plant heat tolerance pathways, and different genotypes have different responses to high-temperature stress.
Article
Engineering, Biomedical
Silvia Sanchez-Casanova, Francisco M. Martin-Saavedra, Clara Escudero-Duch, Maria I. Falguera Uceda, Martin Prieto, Manuel Arruebo, Paloma Acebo, Mario L. Fabiilli, Renny T. Franceschi, Nuria Vilaboa
Review
Immunology
Richard Voellmy, David C. Bloom, Nuria Vilaboa
Editorial Material
Biotechnology & Applied Microbiology
Clara Escudero-Duch, Nuria Vilaboa
Review
Chemistry, Multidisciplinary
Michael T. Cook, Peter Haddow, Stewart B. Kirton, William J. McAuley
Summary: Materials with smart behavior that can change their properties in response to external stimuli have enabled innovative technologies for healthcare applications. Thermoreversible gelators, which undergo reversible phase changes triggered by temperature, are being used for drug delivery, tissue engineering, and 3D printing. Challenges in translating these technologies include the lack of rigorous toxicological evaluation.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Gema Valles, Eduardo Garcia-Rey, Laura Saldana, Eduardo Garcia-Cimbrelo, Nuria Vilaboa
Summary: Aseptic loosening involves inflammation and bone resorption processes. The study found higher serum IGFBP-1 levels in revision surgery patients compared to primary total hip arthroplasty patients, possibly due to increased local IGFBP-1 production in the inflammatory environment of the periprosthetic bed.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Omaima N. Najib, Gary P. Martin, Stewart B. Kirton, Michelle J. Botha, Al-Sayed Sallam, Darragh Murnane
Summary: The study found that certain oils were selectively absorbed and distributed in the model barrier membrane, altering its barrier properties and enhancing the permeation of caffeine, methyl paraben, and butyl paraben.
Article
Chemistry, Medicinal
Daniel Fernandez-Villa, Gerardo Asensio, Manuel Silva, Rosa Ana Ramirez-Jimenez, Laura Saldana, Nuria Vilaboa, Ana Leite-Oliveira, Julio San Roman, Blanca Vazquez-Lasa, Luis Rojo
Summary: The study presented novel vitamin B9 derivatives with divalent cations, which demonstrated bioactivity in modulating osteogenic-like or fibrocartilaginous-like phenotypes. These compounds show potential for further in vivo studies for musculoskeletal regeneration applications.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Rosemond A. Mensah, Seung Bin Jo, Hoon Kim, Sung-Min Park, Kapil D. Patel, Kyong J. Cho, Michael T. Cook, Stewart B. Kirton, Victoria Hutter, Laura E. Sidney, Decio Alves-Lima, Hungyen Lin, Jung-Hwan Lee, Hae-Won Kim, David Y. S. Chau
Summary: The eggshell membrane (ESM) is a natural resource with a complex structure and composition, with potential as a biomaterial for wound dressing applications. This study aimed to identify a reproducible extraction protocol for ESM and assess its properties for wound dressing, demonstrating no calcium residue in the extraction process and excellent biocompatibility with corneal cells, indicating its potential as a novel biomaterial for ophthalmic wounds and other biomedical therapies.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Marcelo Alves da Silva, Peter Haddow, Stewart B. Kirton, William J. McAuley, Lionel Porcar, Cecile A. Dreiss, Michael T. Cook
Summary: Thermoreversible gels transition from liquid to gel upon warming and vice versa upon cooling. A library of ABA copolymers was prepared to evaluate the effect of A chemistry and A/B block molecular weight on thermally-induced phase changes. Rheometry coupled to Small-Angle Neutron Scattering elucidated temperature-dependent hierarchical self-assembly processes and bulk rheology, identifying design principles for next-generation thermoreversible gels.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Peter J. Haddow, Marcelo A. da Silva, Daulet B. Kaldybekov, Cecile A. Dreiss, Ewelina Hoffman, Victoria Hutter, Vitaliy V. Khutoryanskiy, Stewart B. Kirton, Najet Mahmoudi, William J. McAuley, Michael T. Cook
Summary: Thermoreversible gels transitioning between liquid-like and solid-like states have enabled breakthroughs in novel healthcare technologies. This study synthesized ABA copolymers with varying molecular weights of PDEA and PEG blocks, revealing the necessity of high molecular weight PEG blocks for forming solid-like thermoreversible gels. Optimization led to a mucoadhesive, stable, and non-toxic thermoreversible gel with controlled release of hydrophobic drugs, showcasing high promise for healthcare applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Polymer Science
Maria Aguado, Laura Saldana, Eduardo Perez del Rio, Judith Guasch, Marc Parera, Alba Cordoba, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Esther Vazquez, Antonio Villaverde, Jaume Veciana, Imma Ratera, Nuria Vilaboa, Nora Ventosa
Summary: This study demonstrates for the first time the use of Freon R134a for generating porous polymer matrices with larger pore sizes and better mechanical properties compared to conventional scCO(2) processing. MSCs attached to PLA scaffolds processed with Freon R134a exhibited good metabolic activity and displayed spread morphology with a well-organized actin cytoskeleton. Functionalization of Freon R134a-processed PLA scaffolds with protein nanoparticles significantly enhanced the scaffolds' cytocompatibility.
Review
Pharmacology & Pharmacy
Maria I. Falguera Uceda, Silvia Sanchez-Casanova, Clara Escudero-Duch, Nuria Vilaboa
Summary: This review provides an overview of current developments in cell-based strategies for enhancing the surgical armamentarium to regenerate cranial vault defects. The review focuses on cell-based approaches, delivery strategies for extracellular matrix, conditioned media, and extracellular vesicles, as well as recent advances in 3D printing and bioprinting techniques for cranial reconstruction. It also discusses cell-based gene therapy approaches with regulated and unregulated gene switches.
Review
Biochemical Research Methods
Giorgia Vaccaro, Anna Massariol, Amira Guirguis, Stewart B. Kirton, Jacqueline L. Stair
Summary: This paper presents a systematic literature review on the detection of new psychoactive substances (NPS) in prison settings. It provides information on the most frequently reported NPS classes, smuggling routes and forms, and analysis methods for biological and non-biological samples. The study identified synthetic cannabinoids as the most prevalent NPS class in prison settings.
DRUG TESTING AND ANALYSIS
(2022)
Meeting Abstract
Respiratory System
B. J. A. Thorne, S. B. Kirton, M. Knowles, K. C. Lee, D. Murnane, A. I. Sapsford, A. D. Wright
JOURNAL OF AEROSOL MEDICINE AND PULMONARY DRUG DELIVERY
(2021)
Meeting Abstract
Respiratory System
B. J. A. Thorne, S. B. Kirton, D. Murnane, A. I. Sapsford, M. Knowles
JOURNAL OF AEROSOL MEDICINE AND PULMONARY DRUG DELIVERY
(2020)