Article
Multidisciplinary Sciences
Dechamma Pandyanda Nanjappa, Hanna De Saffel, Krithika Kalladka, Srividya Arjuna, Nishith Babu, Kishan Prasad, Patrick Sips, Anirban Chakraborty
Summary: Poly(A)-specific ribonuclease (PARN) is a crucial 3'-5' exonuclease involved in various biological processes, including mRNA deadenylation. Recent studies have revealed that PARN also plays important roles in telomere biology, non-coding RNA maturation, miRNA trimming, and ribosome biogenesis. Furthermore, PARN expression is dysregulated in many cancers, such as solid tumors and hematopoietic malignancies. Using a zebrafish model, researchers found that the loss of PARN function led to the development of only male zebrafish, highlighting its involvement in oogenesis.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Yefan Han, Anni Hu, Yi Qu, Qianqian Xu, Hang Wang, Yin Feng, Yili Hu, Li He, Hao Wu, Xinzhi Wang
Summary: In this study, a specific targeted therapy called HEN-463 was discovered for AML patients with NPM1 gene mutation. HEN-463 inhibits the interaction of LAS1-NOL9 by binding to the C264 site of LAS1, thereby disrupting 28 S rRNA maturation and stabilizing p53. Combining HEN-463 with the XPO1 inhibitor Selinexor enhances the efficacy and overcomes drug resistance.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Engineering, Aerospace
Sergey Rozhkov, Kristina A. Sharlo, Timur M. Mirzoev, Boris S. Shenkman
Summary: The study found that during hindlimb unloading in rat soleus muscle, the expression of regulators/markers of ribosome biogenesis significantly decreased during inactivity, which was accompanied by a progressive decrease in MPS. This suggests that ribosome synthesis plays a crucial role in skeletal muscle inactivity.
Article
Biochemistry & Molecular Biology
Mitsuru Okuwaki, Shoko Saito, Hiroko Hirawake-Mogi, Kyosuke Nagata
Summary: This study revealed that the nucleolar localization of NPM1 and the large ribosomal subunit precursors are mutually dependent. NPM1 plays a crucial role in maintaining the structure of large ribosomal subunits and the accumulation of late processing machinery in the nucleolus.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Plant Sciences
Kaiwei Liu, Keun Pyo Lee, Jianli Duan, Eun Yu Kim, Rahul Mohan Singh, Minghui Di, Zhuoling Meng, Chanhong Kim
Summary: In this study, researchers identified AtRsmD and AtRimM as important factors involved in the 16S rRNA maturation in chloroplasts. AtRsmD catalyzes the m(2)G915 modification of 16S rRNA, while AtRimM is involved in ribosome assembly. These two factors interact with each other and cooperatively regulate the maturation of 16S rRNA and ribosome assembly, contributing to chloroplast proteostasis.
Article
Biochemistry & Molecular Biology
Leonid Anikin, Dimitri G. Pestov
Summary: Aminoacridines, historically used as antiseptic and antiparasitic agents, could be repurposed for therapeutic use and new drug development. In this study, the effects of 9-aminoacridine on pre-rRNA metabolism in mammalian cells were investigated. The results showed that 9-aminoacridine inhibits both pre-rRNA transcription and processing, leading to the disruption of ribosome biogenesis. The ability of 9-aminoacridine to bind to RNA in vitro suggests its potential as a new ribosome biogenesis inhibitor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sohail Khoshnevis, R. Elizabeth Dreggors-Walker, Virginie Marchand, Yuri Motorin, Homa Ghalei
Summary: Protein synthesis by ribosomes is crucial for gene expression in cells, and 2'-O-methylation is a common modification on ribosomal RNAs. This study reveals that changes in the biogenesis of snoRNPs, which guide 2'-O-methylation, lead to the production of ribosomes with distinct translational properties. The hypo-2'-O-methylated ribosomes show translational defects and affect ribosome dynamics and ligand binding. This highlights the importance of 2'-O-methylation in regulating cellular translation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Sergey V. Rozhkov, Kristina A. Sharlo, Boris S. Shenkman, Timur M. Mirzoev
Summary: It has been established that prolonged exposure to microgravity/disuse conditions can lead to a decrease in muscle protein synthesis and muscle mass. This study investigated the effects of inhibiting GSK-3 activity on the downregulation of ribosome biogenesis and muscle protein synthesis in rat soleus muscle during hindlimb suspension. The results showed that inhibition of GSK-3 partially attenuated the reductions in translational capacity and muscle protein synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Zhiyong Liu, Yanan Pang, Yin Jia, Qin Qin, Rui Wang, Wei Li, Jie Jing, Haidong Liu, Shanrong Liu
Summary: This study found that SNORA23 regulates ribosome biogenesis in hepatocellular carcinoma (HCC) by impairing the 2'-O-ribose methylation of 28S rRNA. SNORA23 inhibits the proliferation, migration, and invasion of HCC cells in vitro and in vivo. It is regulated by the PI3K/Akt/mTOR signaling pathway and inhibits the phosphorylation of 4E binding protein 1.
CANCER BIOLOGY & MEDICINE
(2022)
Article
Microbiology
Emma S. V. Andrews, Wayne M. Patrick
Summary: Ribosomes are protein production machines in cells, and the process of ribosome biogenesis is not fully understood. YbeY and YbeZ are two genes involved in RNA-related functions, and they participate together in the maturation of 16S rRNA and protein synthesis. A model is proposed to explain the function of YbeZ in 16S rRNA maturation.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Cell Biology
Claudia Schneider, Katherine E. Bohnsack
Summary: Ribosomes are responsible for translating genetic information into proteins and are composed of rRNAs and RPs. Recent advances in cryo-electron microscopy have allowed the visualization of pre-ribosomal particles, providing new insights into the processing of rRNA and the regulation of ribonucleases. This understanding enhances our knowledge of ribosome assembly and target recognition by ribonucleases.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Plant Sciences
Monika Zakrzewska-Placzek, Anna Golisz-Mocydlarz, Michal Krzyszton, Justyna Piotrowska, Malgorzata Lichocka, Joanna Kufel
Summary: This study demonstrates the involvement of AtNOL12 in rRNA biogenesis and its correlation with stress response in plants. The absence of AtNOL12 alters the expression of ribosomal protein and ribosome biogenesis genes, potentially impacting ribosome function.
Article
Biochemistry & Molecular Biology
Xiao Li, Janice M. Zengel, Lasse Lindahl
Summary: This article describes a novel pathway to the long 5.8S rRNA formation via degradation of ITS1 from its 5' end by exonuclease Xrn1. Mutations in RNase MRP lead to an increase in the accumulation of long 5.8S rRNA relative to short 5.8S rRNA, even when the A3 site is deleted. The link between RNase MRP and 5.8S 5' end formation may involve cleavage at unknown sites elsewhere in pre-rRNA or other RNA molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Rachel McNamar, Katrina Rothblum, Lawrence Rothblum
Summary: Pol I is responsible for transcribing ribosomal DNA genes in eukaryotes, and abnormalities in its activity are linked to cancer and genetic diseases. Studies show differences in Pol I transcription between yeast and mammals, which may affect the function and regulation of PAFs.
Article
Parasitology
Tomas Nepomuceno-Mejia, Luis E. Florencio-Martinez, Isabel Pineda-Garcia, Santiago Martinez-Calvillo
Summary: In this study, a comprehensive analysis was conducted to identify ribosome assembly factors in Leishmania major. By using a combination of purification techniques, mass spectrometry assays, and in silico analysis, the researchers identified several factors involved in the synthesis of ribosomal subunits, including previously unidentified putative orthologs. Additionally, novel candidates for the assembly of the atypical 60S subunit were also discovered. These findings provide important insights into ribosomal biogenesis in L. major and offer potential targets for anti-leishmanial drug development.
Article
Biochemistry & Molecular Biology
Ramtin Shayan, Dana Rinaldi, Natacha Larburu, Laura Plassart, Stephanie Balor, David Bouyssie, Simon Lebaron, Julien Marcoux, Pierre-Emmanuel Gleizes, Celia Plisson-Chastang
Article
Biochemistry & Molecular Biology
Rita Pinto, Cathrine B. Vagbo, Magnus E. Jakobsson, Yeji Kim, Marijke P. Baltissen, Marie-Francoise O'Donohue, Ulises H. Guzman, Jedrzej M. Malecki, Jie Wu, Finn Kirpekar, Jesper Olsen, Pierre-Emmanuel Gleizes, Michiel Vermeulen, Sebastian A. Leidel, Geir Slupphaug, Pal O. Falnes
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Marco Lezzerini, Marianna Penzo, Marie-Francoise O'donohue, Carolina Marques dos Santos Vieira, Manon Saby, Hyung L. Elfrink, Illja J. Diets, Anne-Marie Hesse, Yohann Coute, Marc Gastou, Alexandra Nin-Velez, Peter G. J. Nikkels, Alexandra N. Olson, Evelien Zonneveld-Huijssoon, Marjolijn C. J. Jongmans, GuangJun Zhang, Michel van Weeghel, Riekelt H. Houtkooper, Marcin W. Wlodarski, Roland P. Kuiper, Marc B. Bierings, Jutte van der Werff ten Bosch, Thierry Leblanc, Lorenzo Montanaro, Jonathan D. Dinman, Lydie Da Costa, Pierre-Emmanuel Gleizes, Alyson W. MacInnes
NUCLEIC ACIDS RESEARCH
(2020)
Article
Hematology
Salome Le Goff, Ismael Boussaid, Celia Floquet, Anna Raimbault, Isabelle Hatin, Charlotte Andrieu-Soler, Mohammad Salma, Marjorie Leduc, Emilie-Fleur Gautier, Boris Guyot, Diane d'Allard, Nathalie Montel-Lehry, Sarah Ducamp, Amandine Houvert, Francois Guillonneau, Stephane Giraudier, Elisabeth Cramer-Borde, Francois Morle, Jean-Jacques Diaz, Olivier Hermine, Naomi Taylor, Sandrina Kinet, Frederique Verdier, Rose-Ann Padua, Mohandas Narla, Pierre-Emmanuel Gleizes, Eric Soler, Patrick Mayeux, Michaela Fontenay
Summary: Ribosome biogenesis plays a crucial role in erythroid development, with premature termination impacting erythroid expansion by activating p53 and preventing normal proliferation of erythroid progenitors.
Article
Biochemistry & Molecular Biology
Jedrzej M. Malecki, Marie-Francoise Odonohue, Yeji Kim, Magnus E. Jakobsson, Luca Gessa, Rita Pinto, Jie Wu, Erna Davydova, Anders Moen, Jesper Olsen, Bernd Thiede, Pierre-Emmanuel Gleizes, Sebastian A. Leidel, Pal O. Falnes
Summary: Protein methylation mainly occurs on lysine and arginine, but METTL18, a histidine-specific MTase, plays a crucial role in ribosome biogenesis and function by methylating RPL3. Its knockout results in altered pre-rRNA processing and changes in mRNA translation, highlighting the functional relevance of METTL18 in human cells.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biology
Laura Plassart, Ramtin Shayan, Christian Montellese, Dana Rinaldi, Natacha Larburu, Carole Pichereaux, Carine Froment, Simon Lebaron, Marie-Francoise O'donohue, Ulrike Kutay, Julien Marcoux, Pierre-Emmanuel Gleizes, Celia Plisson-Chastang
Summary: The research shows that the dual key lock mechanism involving RIO1 and RPS26 ensures the precise timing of pre-40S particle conversion into translation-competent ribosomal subunits by displacing DIM2 from the 18S rRNA 30 end and triggering final cleavage by NOB1.
Article
Genetics & Heredity
Simon Lebaron, Marie-Francoise O'donohue, Scott C. Smith, Kendra L. Engleman, Jane Juusola, Nicole P. Safina, Isabelle Thiffault, Carol J. Saunders, Pierre-Emmanuel Gleizes
Summary: This study reports an atypical clinical case of Diamond-Blackfan anemia (DBA) associated with a missense variant in the RPL8 gene. Functional studies showed that these variants affect ribosome production and are likely pathogenic. The study suggests including RPL8 in the list of DBA-associated genes.
Review
Medicine, Research & Experimental
Dechamma Pandyanda Nanjappa, Nishith Babu, Arati Khanna-Gupta, Marie-Francoise O'Donohue, Patrick Sips, Anirban Chakraborty
Summary: PARN is a crucial enzyme in the process of mRNA degradation, with roles beyond regulating mRNA stability in various biological processes. Dysregulated PARN activity is associated with specific disease conditions.
Article
Multidisciplinary Sciences
Gerald Ryan R. Aquino, Philipp Hackert, Nicolai Krogh, Kuan-Ting Pan, Mariam Jaafar, Anthony K. Henras, Henrik Nielsen, Henning Urlaub, Katherine E. Bohnsack, Markus T. Bohnsack
Summary: Early pre-60S ribosomal particles are complex structures undergoing dynamic changes, with RNA helicase Dbp7 playing a key role in regulating the assembly process. Dbp7 influences compaction of domain V/VI and impacts binding and activity of factors like ribosomal protein uL3 in early ribosomal assembly.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Marie-Francoise O'donohue, Lydie Da Costa, Marco Lezzerini, Sule Unal, Clement Joret, Marije Bartels, Eva Brilstra, Marijn Scheijde-Vermeulen, Ludivine Wacheul, Kim De Keersmaecker, Stijn Vereecke, Veerle Labarque, Manon Saby, Sophie D. Lefevre, Jessica Platon, Nathalie Montel-Lehry, Nathalie Laugero, Eric Lacazette, Koen van Gassen, Riekelt H. Houtkooper, Pelin Ozlem Simsek-Kiper, Thierry Leblanc, Nese Yarali, Arda Cetinkaya, Nurten A. Akarsu, Pierre-Emmanuel Gleizes, Denis L. J. Lafontaine, Alyson W. MacInnes
Summary: The study reports that individuals with biallelic HEATR3 variants exhibit bone marrow failure, short stature, dysmorphic features, and intellectual disability, similar to Diamond-Blackfan anemia. These variants destabilize a protein important for nuclear import, impacting ribosomal subunit formation and p53 tumor suppressor stabilization.
Article
Biochemistry & Molecular Biology
Julia Contreras, Oscar Ruiz-Blanco, Carine Dominique, Odile Humbert, Yves Henry, Anthony K. Henras, Jesus de la Cruz, Eduardo Villalobo
Summary: Ribosome synthesis involves various protein trans-acting factors, including DEx(D/H)-box helicases, such as Dbp7. Dbp7 is an RNA helicase that regulates the base-pairing between snR190 small nucleolar RNA and ribosomal RNA during early ribosome biogenesis. The N- and C-terminal domains of Dbp7 are important for its nuclear import, normal growth, and 60S ribosomal subunit synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)