Article
Biochemistry & Molecular Biology
Wei Li, Junwei Wang, Wenjing Hao, Cuifang Yu
Summary: miR-543-3p plays a suppressive role in inflammation, alleviating LPS-induced inflammatory damage by targeting KLF6, which may be beneficial for the treatment of periodontitis.
BIOSCIENCE REPORTS
(2021)
Article
Multidisciplinary Sciences
Lasse K. Markussen, Elizabeth A. Rondini, Olivia Sveidahl Johansen, Jesper G. S. Madsen, Elahu G. Sustarsic, Ann-Britt Marcher, Jacob B. Hansen, Zachary Gerhart-Hines, James G. Granneman, Susanne Mandrup
Summary: This study uncovers the transcriptional network controlled by lipolysis in brown adipocytes and shows that lipolysis is required for the activation of the thermogenic gene program by beta-adrenergic signals. PPARs are identified as key mediators of lipolysis-induced activation of lipid metabolism and thermogenic genes. Additionally, lipolysis also activates the unfolded protein response and regulates the core circadian transcriptional machinery independently of PPARs.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Hyeon-Ji Kang, Byong-Keol Min, Won-Il Choi, Jae-Han Jeon, Dong Wook Kim, Sungmi Park, Yun-Kyung Lee, Hwa-jin Kim, Ju-Eun Byeon, Younghoon Go, Hye Jin Ham, Yong Hyun Jeon, Mi-Jin Kim, Jung Yi Lee, Adam R. Wende, Sung Hee Choi, Robert A. Harris, In-Kyu Lee
Summary: The study revealed that PDK1 and PDK2 are novel regulators of adipogenesis and play critical roles in obesity, with deficiencies offering protection against diet-induced obesity in mice. The enzymes play important roles in the development and growth of fat cells, suggesting that targeting PDK enzymes could be a potential approach to combat obesity.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Medicine, Research & Experimental
Alicia J. Little, Ping-Min Chen, Matthew D. Vesely, Rahanna N. Khan, Jacob Fiedler, James Garritano, Fahrisa I. Maisha, Jennifer M. Mcniff, Joe Craft
Summary: Cutaneous lupus erythematosus (CLE) is an autoimmune skin disease characterized by inflammatory infiltrate of T cells in the skin, however, the mechanism of how these cells adapt and promote tissue inflammation and damage is not known.
Article
Physiology
Claes Fryklund, Bjorn Moren, Shrenika Shah, Mario Grossi, Eva Degerman, Claudia Matthaeus, Karin G. Stenkula
Summary: The study highlights the importance of EHD2 for lipid metabolism, showing that knockout of EHD2 leads to decreased expression of FABP4 and caveolin-1, as well as reduced lipolysis in adipocytes. These findings emphasize differences in mechanisms regulating lipid handling in adipose tissue depots and suggest that impaired lipolysis in EHD2 knockout primary adipocytes likely occurs at the level of, or downstream of, protein kinase A (PKA).
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Timothy M. Moore, Lijing Cheng, Dane M. Wolf, Jennifer Ngo, Mayuko Segawa, Xiaopeng Zhu, Alexander R. Strumwasser, Yang Cao, Bethan L. Clifford, Alice Ma, Philip Scumpia, Orian S. Shirihai, Thomas Q. de Aguiar Vallim, Markku Laakso, Aldons J. Lusis, Andrea L. Hevener, Zhenqi Zhou
Summary: This study reveals the role of Parkin in regulating mitochondrial homeostasis in white adipocytes, suggesting a potential therapeutic target for combating obesity.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Miguel H. Santamaria, Luisa Delgado Rios, Ricardo S. Corral
Summary: Upon infection by Trypanosoma cruzi, adipocytes exhibit an inflammatory phenotype with decreased adiponectin expression, influencing the pathogenesis of Chagas heart disease. T. cruzi interferes with transcriptional regulation of adiponectin production in mouse adipocytes by activating the Ca2+/calcineurin/NFATc4 signaling pathway.
MICROBES AND INFECTION
(2021)
Article
Cardiac & Cardiovascular Systems
Weike Liu, Huajing Song, Jing Xu, Yuqi Guo, Chunju Zhang, Yanli Yao, Hua Zhang, Zhendong Liu, Yue-Chun Li
Summary: This study investigates the mechanism underlying the inhibition of endothelial mitophagy by low blood flow shear stress (SS) through the Cav-1/miR-7-5p/SQSTM1 signaling pathway. The results show that low SS suppresses endothelial mitophagy, leading to impaired mitochondrial homeostasis and endothelial dysfunction.
Article
Multidisciplinary Sciences
Elena Zoico, Nicole Nori, Elena Darra, Maela Tebon, Vanni Rizzatti, Gabriella Policastro, Annamaria De Caro, Andrea Petronio Rossi, Francesco Fantin, Mauro Zamboni
Summary: This study demonstrated the senolytic effect of quercetin on pre-adipocytes and adipocytes in vitro, highlighting its ability to reduce cellular senescence by decreasing beta-galactosidase activity, suppressing ROS, and inhibiting inflammatory cytokines. Additionally, quercetin treatment led to a downregulation of miR-155-5p expression, modulating NF-kappa B activity and showing a trend towards upregulation of SIRT-1, suggesting a potential key mechanism of action for quercetin in reducing adipose tissue aging.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jungin Kwon, Yu-Sheng Yeh, Satoko Kawarasaki, Hiroto Minamino, Yoshihito Fujita, Yuko Okamatsu-Ogura, Haruya Takahashi, Wataru Nomura, Shigenobu Matsumura, Rina Yu, Kazuhiro Kimura, Masayuki Saito, Nobuya Inagaki, Kazuo Inoue, Teruo Kawada, Tsuyoshi Goto
Summary: This study demonstrates the role of the mevalonate (MVA) biosynthesis pathway in regulating brown adipocyte development and survival. Inhibiting the rate-limiting enzyme in the MVA pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), suppresses brown adipocyte differentiation and leads to apoptosis of mature brown adipocytes. The study reveals that geranylgeranyl pyrophosphate (GGPP) generated from the MVA pathway is essential for brown adipocyte development and survival.
Article
Biochemistry & Molecular Biology
Veronika te Boekhorst, Liying Jiang, Marius Mahlen, Maaike Meerlo, Gina Dunkel, Franziska C. Durst, Yanjun Yang, Herbert Levine, Boudewijn M. T. Burgering, Peter Friedl
Summary: This article studies the molecular mechanisms of cancer cell invasion and metastasis under hypoxic conditions and identifies calpain-2 as a key regulator of the transition from collective to amoeboid dissemination in breast and head and neck carcinoma cells. The study reveals that hypoxia induces calpain-2-mediated amoeboid conversion by deactivating beta 1 integrins. Targeted intervention of calpain-2 activity can restore normal cell phenotype and prevent lung metastasis.
Article
Biochemistry & Molecular Biology
Guang-Liang Chen, Rui Li, Xiao-Xiang Chen, Juan Wang, Shan Cao, Rui Song, Ming-Chun Zhao, Li-Ming Li, Nicole Hannemmann, Georg Schett, Cheng Qian, Aline Bozec
Summary: Metastatic melanoma is a challenging disease, and understanding the molecular mechanisms behind its metastasis is crucial. The downregulation of AP-1 transcription factor member Fra-2 in melanoma cells is associated with a more aggressive phenotype both in vitro and in vivo. Further research revealed that the Fra-2-Fam212b axis plays a key role in promoting melanoma metastasis by affecting cell migration, invasion, cell cycle transition, and tissue angiogenesis.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Charlotte Zoe Angel, Mei Yu Cynthia Stafford, Christopher J. J. McNally, Heather Nesbitt, Declan J. J. McKenna
Summary: This study investigates the link between hypoxia and microRNA-21 (miR-21) in prostate cancer cells. It is found that hypoxia can induce the up-regulation of miR-21 expression in prostate cells, which promotes the progression of prostate cancer by suppressing the expression of the tumor suppressor gene RHOB. Additionally, miR-21 shows potential as a biomarker of hypoxia that can aid in the diagnosis and prognosis of prostate cancer.
Article
Cell Biology
Gwan-Jun Lee, Youn Ju Kim, Bongju Park, Sujin Yim, Chansang Park, Hyunsoo Roh, Yunwon Moon, Je Kyung Seong, Hyunsung Park
Summary: This study found that adipocytes are primarily responsible for the increase in Wnt5a levels in obesity. Adipocytes in subcutaneous white adipose tissues are more sensitive to high-fat diet and have a positive correlation between cell size and Wnt5a levels. YAP and TAZ activity responds to mechanical changes in hypertrophic adipocytes to promote Wnt5a expression.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Zhaoxing Dong, Ting Li, Cenli Wang, Yong Zhou, Zhongkai Tong, Xuekui Du
Summary: Sestrin2 alleviates pulmonary fibrosis and endoplasmic reticulum stress-dependent ferroptosis through the NRF2/ATF4 pathway, improving inflammation and promoting cell proliferation.
JOURNAL OF IMMUNOLOGY RESEARCH
(2023)
Article
Urology & Nephrology
Julie Contenti, Matthieu Durand, Sandor Vido, Serge Declemy, Juliette Raffort, Joseph Carboni, Sophie Bonnet, Christophe Koelsch, Reda Hassen-Khodja, Philippe Gual, Nathalie M. Mazure, Nirvana Sadaghianloo
Summary: The study found that the level of pOPN in hemodialysis patients was associated with the presence of AVF stenosis requiring intervention, suggesting its potential as a diagnostic biomarker for evaluation in a vascular access surveillance program.
JOURNAL OF NEPHROLOGY
(2022)
Article
Gastroenterology & Hepatology
Carmelo Luci, Elodie Vieira, Manon Bourinet, Deborah Rousseau, Stephanie Bonnafous, Stephanie Patouraux, Lauren Lefevre, Frederic Larbret, Virginie Prod'homme, Antonio Iannelli, Albert Tran, Rodolphe Anty, Beatrice Bailly-Maitre, Marcel Deckert, Philippe Gual
Summary: The SYK-3BP2 pathway plays an important role in the pathogenesis of chronic liver inflammatory diseases. Targeting the SYK-3BP2 pathway in myeloid cells and hepatocytes can effectively control liver inflammation and fibrosis, which may be a potential strategy for treating metabolic steatohepatitis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Simon Lecoutre, Karine Clement, Isabelle Dugail
Summary: Adipose tissue dysfunction is closely associated with obesity and its metabolic complications. It has been discovered that adipocytes have a tendency to extrude damaged mitochondria into the extracellular space, which can then be transported through the blood and taken up by other cells. This phenomenon has significant implications in the context of obesity-related adipose tissue dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Laura Sormani, Henri Montaudie, Lauriane Blot, Marjorie Heim, Nathalie Cardot Leccia, Rana Mhaidly, Els Verhoeyen, Claire Regazzetti, Nicolas Nottet, Yann Cheli, Gian Marco De Donatis, Anne Sophie Dabert Gay, Delphine Debayle, Helene Taquin Martin, Franck Gesbert, Stephane Rocchi, Meri K. Tulic, Thierry Passeron
Summary: Pigmentation of human skin is regulated by multiple genes, and this study reveals the importance of CLEC12B gene in melanocytes and its regulatory mechanism. CLEC12B activates SHP1 and SHP2 proteins, leading to the degradation of CRE-binding protein, ultimately controlling melanin production and pigmentation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Cell Biology
Simona Cataldi, Marianna Aprile, Daniela Melillo, Ines Mucel, Sophie Giorgetti-Peraldi, Mireille Cormont, Paola Italiani, Matthias Blueher, Jean-Francois Tanti, Alfredo Ciccodicola, Valerio Costa
Summary: In this study, it was found that inflammatory factors secreted by metabolically-activated macrophages can modulate the expression and splicing of PPARG, leading to impaired adipocyte differentiation and exacerbating the defective PPAR gamma activity in hypertrophic adipose tissue.
Article
Pathology
Mathieu Roumiguie, David Esteve, Cecile Manceau, Aurelie Toulet, Jerome Gilleron, Chloe Belles, Yiyue Jia, Cynthia Houel, Sarah Pericart, Sophie LeGonidec, Philippe Valet, Mireille Cormont, Jean-Francois Tanti, Bernard Malavaud, Anne Bouloumie, Delphine Milhas, Catherine Muller
Summary: This study characterized the structural and functional features of periprostatic adipose tissue (PPAT) and found that it has a sparse vascular network compared to abdominopelvic adipose tissue (APAT), suggesting hypoxia in PPAT. The gene expression analysis revealed high levels of hypoxia-related genes in PPAT, confirming its adaptive response to hypoxia. This chronic hypoxic state is associated with inflammation and fibrosis, explaining why PPAT does not expand in obesity.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Gastroenterology & Hepatology
Agathe Beaulant, Maya Dia, Bruno Pillot, Marie-Agnes Chauvin, Jingwei Ji-cao, Christine Durand, Nadia Bendridi, Stephanie Chanon, Aurelie Vieille-Marchiset, Claire Crola Da Silva, Stephanie Patouraux, Rodolphe Anty, Antonio Iannelli, Albert Tran, Philippe Gual, Hubert Vidal, Ludovic Gomez, Melanie Paillard, Jennifer Rieusset
Summary: The study reveals that ER-mitochondria miscommunication is an early trigger of hepatic insulin resistance and steatosis in obese mice, which can be improved by switching to a healthy diet. Manipulating organelle communication in the liver can impact hepatic metabolic functions.
JOURNAL OF HEPATOLOGY
(2022)
Article
Cell Biology
Romain Donne, Maeva Saroul-Ainama, Pierre Cordier, Adel Hammoutene, Christelle Kabore, Mira Stadler, Ivan Nemazanyy, Isabelle Galy-Fauroux, Mounia Herrag, Tobias Riedl, Marie Chansel-Da Cruz, Stefano Caruso, Stephanie Bonnafous, Rupert Ollinger, Roland Rad, Kristian Unger, Albert Tran, Jean-Pierre Couty, Philippe Gual, Valerie Paradis, Severine Celton-Morizur, Mathias Heikenwalder, Patrick Revy, Chantal Desdouets
Summary: This study investigated the mechanisms of DNA damage response during NAFLD and found that nucleotide pool imbalance is the key driver of replication stress (RS), and DNA damage also activates the cGAS/STING pathway, promoting disease progression.
DEVELOPMENTAL CELL
(2022)
Article
Endocrinology & Metabolism
Simon Lecoutre, Fatiha Merabtene, Elie-Julien El Hachem, Camille Gamblin, Christine Rouault, Nataliya Sokolovska, Hedi Soula, Wi S. Lai, Perry J. Blackshear, Karine Clement, Isabelle Dugail
Summary: The study reveals the potential of beta-hydroxybutyrate to limit adipose tissue matrix deposition, which is significant in the context of obesity-related tissue dysfunction.
MOLECULAR METABOLISM
(2022)
Review
Cell Biology
Simon Lecoutre, Melanie Lambert, Krzysztof Drygalski, Isabelle Dugail, Salwan Maqdasy, Mathieu Hautefeuille, Karine Clement
Summary: Adipose tissue expansion is an adaptive mechanism that increases nutrient buffering capacity. However, remodeling of adipose tissue, characterized by adipocyte hypertrophy, immune cells infiltration, fibrosis, and changes in vascular architecture, generates mechanical stress on adipose cells. This mechanical stimulus alters adipose function through mechanotransduction, leading to obesity-associated dysfunction in adipose tissue.
Article
Cell Biology
Pierre-Jean Cornejo, Bastien Vergoni, Mickael Ohanna, Brice Angot, Teresa Gonzalez, Jennifer Jager, Jean-Francois Tanti, Mireille Cormont
Summary: This study identifies miR-34a as a new mediator of adipocyte insulin resistance. Upregulation of miR-34a inhibits insulin signaling and decreases glucose transport, accompanied by a reduced expression of VAMP2 and an increased expression of PTP1B. Mechanistically, miR-34a decreases NAD(+) levels, thereby inhibiting Sirtuin-1 and promoting the upregulation of PTP1B. The mRNA expression of Nampt and Naprt is also decreased in adipose tissue of obese mice.
Editorial Material
Endocrinology & Metabolism
Isabelle Dugail, Soazig Le Lay
Article
Endocrinology & Metabolism
Krzysztof Drygalski, Simon Lecoutre, Karine Clement, Isabelle Dugail
Summary: Hyaluronic acid, a hydrophilic substance used as a dermal filler, has recently been recognized for its role in inflammatory response, insulin resistance, adipogenesis, and autoimmunity. Despite its importance, the understanding of its potential role in the extracellular environment is still limited. This article emphasizes the underestimated implications of hyaluronic acid in adipose tissue function, adipogenesis, and obesity-related dysfunction based on its unique physical property as a regulator of osmotic pressure.
Article
Biochemistry & Molecular Biology
Ainara Cano, Mercedes Vazquez-Chantada, Javier Conde-Vancells, Aintzane Gonzalez-Lahera, David Mosen-Ansorena, Francisco J. J. Blanco, Karine Clement, Judith Aron-Wisnewsky, Albert Tran, Philippe Gual, Carmelo Garcia-Monzon, Joan Caballeria, Azucena Castro, Maria Luz Martinez-Chantar, Jose M. Mato, Huiping Zhu, Richard H. H. Finnell, Ana M. M. Aransay
Summary: Low serum folate levels are associated with metabolic associated fatty liver disease (MAFLD). The role of the folate transporter gene (SLC19A1) in lipid accumulation during the onset of MAFLD was assessed through genomic, transcriptomic, and metabolomic techniques. SNPs rs1051266 and rs3788200 were significantly associated with fatty liver development, and the lack of functional SLC19A1 affected gene regulation and lipid metabolism, leading to lipid droplet accumulation in hepatocytes.