Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

Whole cell maps chart a course for 21st-century cell biology

(2017) Rick Horwitz et al.   SCIENCE

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

(2017) Carl W. White et al.   Scientific Reports

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions

(2016) William T. Hendriks et al.   Cell Stem Cell

Induced Pluripotent Stem Cells Meet Genome Editing

(2016) Dirk Hockemeyer et al.   Cell Stem Cell

Organization of Non-centrosomal Microtubules in Epithelial Cells

(2016) Mika Toya et al.   CELL STRUCTURE AND FUNCTION

Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives

(2016) Fabian Oceguera-Yanez et al.   METHODS

Distinct patterns of Cas9 mismatch tolerancein vitroandin vivo

(2016) Becky X.H. Fu et al.   NUCLEIC ACIDS RESEARCH

A scalable strategy for high-throughput GFP tagging of endogenous human proteins

(2016) Manuel D. Leonetti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Versatile protein tagging in cells with split fluorescent protein

(2016) Daichi Kamiyama et al.   Nature Communications

Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration

(2016) Zhuo Gan et al.   Cell Systems

A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells

(2016) Scott D. Findlay et al.   PLoS One

Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases

(2016) Diane Yang et al.   Scientific Reports

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

(2016) Yuichiro Miyaoka et al.   Scientific Reports

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

(2016) Won-Ki Cho et al.   Scientific Reports

Inhibition of  -catenin signaling respecifies anterior-like endothelium into beating human cardiomyocytes

(2015) N. J. Palpant et al.   DEVELOPMENT

Chemically defined, albumin-free human cardiomyocyte generation

(2015) Xiaojun Lian et al.   NATURE METHODS

CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells

(2015) Michael Ratz et al.   Scientific Reports

Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus

(2015) Florian T. Merkle et al.   Cell Reports

cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications

(2015) Behnam Ahmadian Baghbaderani et al.   Stem Cell Reports

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Inherited desmosomal disorders

(2014) Liat Samuelov et al.   CELL AND TISSUE RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Genome maintenance in pluripotent stem cells

(2014) Uri Weissbein et al.   JOURNAL OF CELL BIOLOGY

Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis

(2014) Alexandre Grassart et al.   JOURNAL OF CELL BIOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

SOX17 links gut endoderm morphogenesis and germ layer segregation

(2014) Manuel Viotti et al.   NATURE CELL BIOLOGY

Advances in fluorescence labeling strategies for dynamic cellular imaging

(2014) Kevin M Dean et al.   Nature Chemical Biology

Isolation of single-base genome-edited human iPS cells without antibiotic selection

(2014) Yuichiro Miyaoka et al.   NATURE METHODS

International Coordination of Large-Scale Human Induced Pluripotent Stem Cell Initiatives: Wellcome Trust and ISSCR Workshops White Paper

(2014) Filipa A.C. Soares et al.   Stem Cell Reports

Mechanistic Basis of Desmosome-Targeted Diseases

(2013) Caezar Al-Jassar et al.   JOURNAL OF MOLECULAR BIOLOGY

The transience of transient overexpression

(2013) Toby J Gibson et al.   NATURE METHODS

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Reconsidering pluripotency tests: Do we still need teratoma assays?

(2013) Christiane Buta et al.   Stem Cell Research

Repair of Strand Breaks by Homologous Recombination

(2013) M. Jasin et al.   Cold Spring Harbor Perspectives in Biology

Repair of Double-Strand Breaks by End Joining

(2013) K. K. Chiruvella et al.   Cold Spring Harbor Perspectives in Biology

Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1

(2013) QING CHEN et al.   Experimental and Therapeutic Medicine

The Presenilin-1 ΔE9 Mutation Results in Reduced γ-Secretase Activity, but Not Total Loss of PS1 Function, in Isogenic Human Stem Cells

(2013) Grace Woodruff et al.   Cell Reports

STAR: ultrafast universal RNA-seq aligner

(2012) Alexander Dobin et al.   BIOINFORMATICS

Alzheimer's disease in a dish: promises and challenges of human stem cell models

(2012) J. E. Young et al.   HUMAN MOLECULAR GENETICS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations

(2011) Frank Soldner et al.   CELL

Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells

(2011) Jeffrey B. Doyon et al.   NATURE CELL BIOLOGY

Microtubule nucleation by γ-tubulin complexes

(2011) Justin M. Kollman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Targeted Genome Editing Across Species Using ZFNs and TALENs

(2011) Andrew J. Wood et al.   SCIENCE

Pax6 Is a Human Neuroectoderm Cell Fate Determinant

(2010) Xiaoqing Zhang et al.   Cell Stem Cell

Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation

(2010) Cole Trapnell et al.   NATURE BIOTECHNOLOGY

Aurora B-Mediated Abscission Checkpoint Protects against Tetraploidization

(2009) Patrick Steigemann et al.   CELL

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Differentiation of Embryonic Stem Cells to Clinically Relevant Populations: Lessons from Embryonic Development

(2008) Charles E. Murry et al.   CELL

The Reticulon and Dp1/Yop1p Proteins Form Immobile Oligomers in the Tubular Endoplasmic Reticulum

(2008) Yoko Shibata et al.   JOURNAL OF BIOLOGICAL CHEMISTRY