Letter
Plant Sciences
Xiaojing Liu, Weijia Cheng, Peng Yao, Kexin Ren, Yu Wang, Yingnan Sun, Xin Hou, Li Lu, Xiangsong Chen
Summary: Conserved serine phosphorylation regulates histone deacetylase activity in Arabidopsis and humans.
Review
Genetics & Heredity
Gaelle Pennarun, Julien Picotto, Pascale Bertrand
Summary: The nuclear envelope plays essential roles in protecting the genome and also in various important functions, including chromatin organization and DNA replication and repair. Changes in the nuclear envelope are associated with multiple human diseases and cancer cells. Telomeres, which are crucial for genome stability, interact with the nuclear envelope in yeast, where their tethering to the envelope is critical for their maintenance. Recent studies have revealed close ties between mammalian telomeres and the nuclear envelope, highlighting their important roles in maintaining genome integrity.
Article
Multidisciplinary Sciences
Yukti Hari-Gupta, Natalia Fili, Alia dos Santos, Alexander W. Cook, Rosemarie E. Gough, Hannah C. W. Reed, Lin Wang, Jesse Aaron, Tomas Venit, Eric Wait, Andreas Grosse-Berkenbusch, J. Christof M. Gebhardt, Piergiorgio Percipalle, Teng-Leong Chew, Marisa Martin-Fernandez, Christopher P. Toseland
Summary: This study reveals the crucial role of nuclear myosin VI (MVI) in the spatial organization of RNA polymerase II (RNAPII). MVI acts as a molecular anchor to maintain high-density clusters of RNAPII. Perturbation of MVI disrupts RNAPII localization, chromatin organization, and gene expression.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Tadayuki Komori, Tomoki Kuwahara, Tetta Fujimoto, Maria Sakurai, Ikuko Koyama-Honda, Mitsunori Fukuda, Takeshi Iwatsubo
Summary: Recent studies have identified Rab29 as a Rab protein phosphorylated by LRRK2 and found that it is also phosphorylated under lysosomal overload stress. The phosphorylation site is Ser185 and this phosphorylation is involved in counteracting lysosomal enlargement. PKCα and PKCδ are involved in this phosphorylation and control the lysosomal localization of Rab29.
JOURNAL OF CELL SCIENCE
(2023)
Article
Andrology
Kun Li, Peibei Sun, Yayan Wang, Tian Gao, Dongwang Zheng, Ajuan Liu, Ya Ni
Summary: This study revealed the interaction between Hsp90 and Cdc37 in human spermatozoa and showed that PKA and PKC signaling pathways regulate total protein threonine phosphorylation and Src phosphorylation during sperm capacitation. The results help to understand the mechanism underlying Hsp90 regulation of sperm function.
Article
Biochemistry & Molecular Biology
Laura A. Murray-Nerger, Joshua L. Justice, Pranav Rekapalli, Josiah E. Hutton, Ileana M. Cristea
Summary: Acetylation of lamin B1 at K134 is a molecular switch that controls nuclear periphery stability, cell cycle progression, and DNA repair. This modification prevents lamin disruption during HSV-1 infection, delays DNA damage resolution, and sustains activation of the G1/S checkpoint. Acetylation of LMNB1 negatively regulates canonical nonhomologous end joining and impacts DNA repair pathway choice.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yohei Mizoguchi, Kaoru Nakashima, Ayato Sato, Asako Shindo
Summary: This study reveals the important contribution of ADRB2 to the extensibility of epithelial tissues and body elongation during development, as well as its impact on the physiology of mature epithelial organs.
Article
Multidisciplinary Sciences
Svetlana Minakhina, Vanessa De Oliveira, Sun Young Kim, Haiyan Zheng, Fredric E. Wondisford
Summary: Fasting induces significant changes in the HPT axis, with THRB2 S101 phosphorylation playing a key role in the repression of the axis by TH and AMPK activation. CDK2 is proposed as a direct kinase phosphorylating THRB2 S101, suggesting a common mechanism for acute suppression of the HPT axis by nutritional deprivation and TH.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Yingying Zou, Jiayao Pei, Haizhen Long, Liting Lan, Kejian Dong, Tingting Wang, Ming Li, Zhexuan Zhao, Lirun Zhu, Gangxuan Zhang, Xin Jin, Yang Wang, Zengqi Wen, Min Wei, Yunpeng Feng
Summary: Using proteomics, molecular biology and sequencing, the authors demonstrate the importance of OGT-dependent O-GlcNAcylation of H4S47 in the activation of replication origins. This occurs through regulation of DDK-induced phosphorylation of the MCM complex. The findings suggest that H4S47 O-GlcNAcylation enhances replication initiation by facilitating MCM phosphorylation, providing insight into the control of replication efficiency by the chromatin environment.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Simona Graziano, Nuria Coll-Bonfill, Barbara Teodoro-Castro, Sahiti Kuppa, Jessica Jackson, Elena Shashkova, Urvashi Mahajan, Alessandro Vindigni, Edwin Antony, Susana Gonzalo
Summary: Lamin A/C plays a crucial role in DNA replication by binding to nascent DNA and recruiting replication fork protective factors. Depletion of Lamin A/C leads to replication fork instability, characterized by degradation of nascent DNA, DNA damage, and sensitivity to replication inhibitors. This instability is rescued by exogenous overexpression of RPA or RAD51, emphasizing the importance of Lamin A/C in maintaining genome stability.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Matteo Lulli, Laura Del Coco, Tommaso Mello, Caecilia Sukowati, Stefania Madiai, Laura Gragnani, Paolo Forte, Francesco Paolo Fanizzi, Antonio Mazzocca, Krista Rombouts, Andrea Galli, Vinicio Carloni
Summary: This study reveals that CHK2, a central effector of DNA damage response, regulates cellular energy production by affecting glycolysis and mitochondrial functions. Patients with hepatocellular carcinoma (HCC) showed increased CHK2 mRNA levels in blood, which correlated with elevated TCA metabolites, indicating the role of CHK2 in controlling mitochondrial functions.
Article
Biochemistry & Molecular Biology
Christoph Schmal, Bert Maier, Reut Ashwal-Fluss, Osnat S. Bartok, Anna-Marie Finger, Tanja Bange, Stella Koutsouli, Maria S. Robles, Sebastian Kadener, Hanspeter Herzel, Achim Kramer
Summary: A defining property of circadian clocks is temperature compensation, which refers to the resilience of their near-24-hour free-running periods against changes in environmental temperature. This study investigates the molecular mechanisms underlying temperature compensation by knocking down CPSF6 in human cells. The researchers use RNA sequencing and proteomics to identify changes in gene and protein expression, as well as 3' UTR length, in response to temperature and reveal candidate genes involved in circadian temperature compensation.
Article
Biochemistry & Molecular Biology
Iram Fatima, Susmita Barman, JayaPrakash Uppada, Shailender Chauhan, Sanchita Rauth, Satyanarayana Rachagani, Moorthy Palanimuthu Ponnusamy, Lynette Smith, Geoffrey Talmon, Amar B. Singh, Surinder K. Batra, Punita Dhawan
Summary: This study reveals a critical role of MASTL in promoting pancreatic cancer malignancy and therapy resistance by modulating protein stability and kinase signaling. Combinatorial therapy targeting MASTL enhances the cell-killing effects of Gemcitabine, demonstrating its potential as a novel target to improve sensitivity to anti-pancreatic cancer therapies.
Article
Biochemistry & Molecular Biology
Zhi-Da Zhang, Bing Wen, Da-Jia Li, Dan-Xia Deng, Xiao-Dong Wu, Yin-Wei Cheng, Lian-Di Liao, Lin Long, Geng Dong, Li-Yan Xu, En-Min Li
Summary: In this study, we identified a novel phosphorylation site in Fascin and confirmed AKT2 as the kinase for this site. Phosphorylation of Fascin Threonine 403 suppresses the behavior of esophageal cancer cells, actin-bundling activity, and filopodia formation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dhirendra K. Sharma, Subhash C. Bihani, Mohammad Q. Siddiqui, Hari S. Misra, Yogendra S. Rajpurohit
Summary: RqkA is a DNA damage responsive serine/threonine kinase with a unique structure in D. radiodurans. The WD40 domain in RqkA plays a crucial role in regulating its kinase signaling functions, interaction, and activation of kinase activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Cell Biology
Daniel L. Levy, Rebecca Heald
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2016)
Review
Developmental Biology
Predrag Jevtic, Ana Milunovic-Jevtic, Matthew R. Dilsaver, Jesse C. Gatlin, Daniel L. Levy
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2016)
Article
Cell Biology
Ana Milunovic-Jevtic, Predrag Jevtic, Daniel L. Levy, J. C. Gatlin
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Multidisciplinary Sciences
Predrag Jevtic, Daniel L. Levy
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Matthew R. Dilsaver, Pan Chen, Trey A. Thompson, Traci Reusser, Richik N. Mukherjee, John Oakey, Daniel L. Levy
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Biochemistry & Molecular Biology
Predrag Jevtic, Andria C. Schibler, Chase C. Wesley, Gianluca Pegoraro, Tom Misteli, Daniel L. Levy
Article
Cell Biology
Richik Nilay Mukherjee, Daniel L. Levy
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Multidisciplinary Sciences
Predrag Jevtic, Richik N. Mukherjee, Pan Chen, Daniel L. Levy
Article
Cell Biology
Pan Chen, Miroslav Tomschik, Katherine M. Nelson, John Oakey, Jesse C. Gatlin, Daniel L. Levy
JOURNAL OF CELL BIOLOGY
(2019)
Review
Developmental Biology
Chase C. Wesley, Sampada Mishra, Daniel L. Levy
WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY
(2020)
Article
Biology
Predrag Jevtic, K. Wade Elliott, Shelby E. Watkins, Jonathan A. Sreter, Katarina Jovic, Ian B. Lehner, Paul W. Baures, John G. Tsavalas, Daniel L. Levy, Krisztina Varga
Summary: Cryoprotection is important in various research fields. Antifreeze proteins, as an evolutionary adaptation, can provide cryoprotection to certain organisms. By investigating the effects of an antifreeze protein from the desert beetle in Xenopus laevis, it was found to reduce damage and increase survival.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Cell Biology
Sampada Mishra, Daniel L. Levy
Summary: Nuclear shape influences various cellular processes and is altered in certain diseases. This study explores the factors and forces that determine nuclear shape. The findings suggest that the interaction between nuclear F-actin and Lamin A contributes to the regulation of nuclear shape, and targeting nuclear actin dynamics may be a potential approach for rescuing disease-associated defects in nuclear shape.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Andria C. Schibler, Predrag Jevtic, Gianluca Pegoraro, Daniel L. Levy, Tom Misteli
Summary: The size and shape of the human cell nucleus varies greatly among different cell types and tissues. Changes in nuclear morphology are associated with diseases including cancer, as well as aging. However, the factors that determine nuclear shape and size are not well understood. Through a comprehensive screening, we identified a set of novel regulators of nuclear morphology and found that the interaction between lamin A and histone H3 plays an important role in nuclear shape.
Article
Cell Biology
Chase C. Wesley, Daniel L. Levy
Summary: The nuclear lamina plays important roles in chromatin organization and structural support. However, its structural changes during cellular differentiation and its impact on gene regulation are not well understood. In this study, we investigated the dynamics of lamin B1 in human-induced pluripotent stem cells (iPSCs) and differentiated cells derived from different germ layers. We found that lamin B1 dynamics increase during iPSCs differentiation, especially in mesoderm and ectoderm, and that lamin B receptor (LBR) redistributes from the nucleus to cytoplasm in mesoderm. Knocking down LBR in iPSCs led to increased lamin B1 dynamics and altered expression of differentiation markers. These findings suggest that differentiation-dependent tethering of lamin B1, mediated by LBR or LBR-chromatin associations, affects gene expression.
MOLECULAR BIOLOGY OF THE CELL
(2023)