期刊
MOLECULAR BIOLOGY OF THE CELL
卷 28, 期 22, 页码 3043-3056出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E17-01-0021
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资金
- VICTR [2ULTR000445]
- NIH [P30DK058404, P50CA095103, R01DK48370, R01DK70856, R01DK075555, R01DK095811]
- AACR-Landon Foundation [15-20-27-LAUK]
- Crohn's and Colitis Foundation CDA [308221]
- VICTR from the NIH [UL1TR000445]
- Burroughs Wellcome Fund
- [R01DK103831]
Flow of fluids through the gut, such as milk from a neonatal diet, generates a shear stress on the unilaminar epithelium lining the lumen. We report that exposure to physiological levels of fluid shear stress leads to the formation of large vacuoles, containing extracellular contents within polarizing intestinal epithelial cell monolayers. These observations lead to two questions: how can cells lacking primary cilia transduce shear stress, and what molecular pathways support the formation of vacuoles that can exceed 80% of the cell volume? We find that shear forces are sensed by actin-rich microvilli that eventually generate the apical brush border, providing evidence that these structures possess mechanosensing ability. Importantly, we identified the molecular pathway that regulates large vacuole formation downstream from mechanostimulation to involve central components of the autophagy pathway, including ATG5 and LC3, but not Beclin. Together our results establish a novel link between the actin-rich microvilli, the macroscopic transport of fluids across cells, and the noncanonical autophagy pathway in organized epithelial monolayers.
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