Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Orthogonal Cysteine Protection Enables Homogeneous Multi-Drug Antibody-Drug Conjugates

(2016) Matthew R. Levengood et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

(2016) Dona York et al.   BMC BIOTECHNOLOGY

Cutting-edge mass spectrometry methods for the multi-level structural characterization of antibody-drug conjugates

(2016) Alain Beck et al.   Expert Review of Proteomics

Insights from native mass spectrometry and ion mobility-mass spectrometry for antibody and antibody-based product characterization

(2016) Guillaume Terral et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Antibodies to watch in 2017

(2016) Janice M. Reichert   mAbs

Pharmacokinetics and pharmacodynamics of DSTA4637A: A novel THIOMAB™ antibody antibiotic conjugate against Staphylococcus aureus in mice

(2016) Chenguang Zhou et al.   mAbs

RN927C, a Site-Specific Trop-2 Antibody-Drug Conjugate (ADC) with Enhanced Stability, Is Highly Efficacious in Preclinical Solid Tumor Models

(2016) P. Strop et al.   MOLECULAR CANCER THERAPEUTICS

Efficient Payload Delivery by a Bispecific Antibody-Drug Conjugate Targeting HER2 and CD63

(2016) B. E. C. G. de Goeij et al.   MOLECULAR CANCER THERAPEUTICS

Mechanisms of Resistance to Antibody–Drug Conjugates

(2016) Frank Loganzo et al.   MOLECULAR CANCER THERAPEUTICS

Site-Specific Tandem Knoevenagel Condensation–Michael Addition To Generate Antibody–Drug Conjugates

(2016) Romas A. Kudirka et al.   ACS Medicinal Chemistry Letters

Next-Generation Antibody-Drug Conjugates (ADCs) for Cancer Therapy

(2016) Jagath Reddy Junutula et al.   ACS Medicinal Chemistry Letters

Collision Induced Unfolding of Intact Antibodies: Rapid Characterization of Disulfide Bonding Patterns, Glycosylation, and Structures

(2015) Yuwei Tian et al.   ANALYTICAL CHEMISTRY

CIUSuite: A Quantitative Analysis Package for Collision Induced Unfolding Measurements of Gas-Phase Protein Ions

(2015) Joseph D. Eschweiler et al.   ANALYTICAL CHEMISTRY

MAPN: First-in-Class Reagent for Kinetically Resolved Thiol-to-Thiol Conjugation

(2015) Oleksandr Koniev et al.   BIOCONJUGATE CHEMISTRY

CBTF: New Amine-to-Thiol Coupling Reagent for Preparation of Antibody Conjugates with Increased Plasma Stability

(2015) Sergii Kolodych et al.   BIOCONJUGATE CHEMISTRY

Field Guide to Challenges and Opportunities in Antibody–Drug Conjugates for Chemists

(2015) Mallory R. Gordon et al.   BIOCONJUGATE CHEMISTRY

Generating Site-Specifically Modified Proteins via a Versatile and Stable Nucleophilic Carbon Ligation

(2015) Romas Kudirka et al.   CHEMISTRY & BIOLOGY

Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

(2015) Patrick G. Holder et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugate

(2015) Julien Marcoux et al.   PROTEIN SCIENCE

Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment

(2014) François Debaene et al.   ANALYTICAL CHEMISTRY

Dynamics of Intact Immunoglobulin G Explored by Drift-Tube Ion-Mobility Mass Spectrometry and Molecular Modeling

(2014) Kamila J. Pacholarz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic Fc Glycosylation via Engineered Aldehyde Tags

(2014) Elizabeth L. Smith et al.   BIOCONJUGATE CHEMISTRY

Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes

(2014) Penelope M. Drake et al.   BIOCONJUGATE CHEMISTRY

Exploring the effects of linker composition on site-specifically modified antibody–drug conjugates

(2014) Aaron E. Albers et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Site-Specific Trastuzumab Maytansinoid Antibody–Drug Conjugates with Improved Therapeutic Activity through Linker and Antibody Engineering

(2014) Thomas H. Pillow et al.   JOURNAL OF MEDICINAL CHEMISTRY

Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates

(2013) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

Mass Spectrometric Characterization of Transglutaminase Based Site-Specific Antibody–Drug Conjugates

(2013) Santiago E. Farias et al.   BIOCONJUGATE CHEMISTRY

Site-specific antibody drug conjugates for cancer therapy

(2013) Siler Panowski et al.   mAbs

Antibody-drug conjugates

(2013) Alain Beck et al.   mAbs

Considerations for the nonclinical safety evaluation of antibody drug conjugates for oncology

(2013) Stanley A. Roberts et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Fourth World Antibody-Drug Conjugate Summit

(2012) Alain Beck et al.   mAbs

Site-specific chemical protein conjugation using genetically encoded aldehyde tags

(2012) David Rabuka et al.   Nature Protocols

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Collision Cross Sections of Proteins and Their Complexes: A Calibration Framework and Database for Gas-Phase Structural Biology

(2010) Matthew F. Bush et al.   ANALYTICAL CHEMISTRY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

Ion mobility–mass spectrometry analysis of large protein complexes

(2008) Brandon T Ruotolo et al.   Nature Protocols