Article
Cell & Tissue Engineering
Yu-Hsun Chang, V. Bharath Kumar, Yao-Tseng Wen, Chih-Yang Huang, Rong-Kung Tsai, Dah-Ching Ding
Summary: This study demonstrates the successful differentiation of human umbilical cord mesenchymal stem cells (HUCMSCs) into retinal pigment epithelial (RPE)-like cells using a co-culture system. The induced cells showed the characteristic markers and functions of RPE cells, including engulfing photoreceptor outer segments and secreting neurotrophic factors. These findings provide fundamental information for the clinical-scale generation of RPE-like cells from HUCMSCs.
CELL TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Qiaoyun Gong, Dawei Luo, Haiyan Wang, Xun Xu, Ying Fan, Zhi Zheng, Tianwei Qian
Summary: Researchers studying diabetic retinopathy (DR) have become interested in the catabolic process of autophagy, but its role and molecular mechanism in DR are still unclear. In this study, an in vivo diabetic rat model and in vitro hyperglycemic-exposed retinal pigment epithelium (RPE) cell cultures were used to mimic early DR. It was found that miR-19a-3p upregulation inhibits aberrant autophagy by directly targeting PTEN, thus protecting RPE cells against DR damage.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mei Yang, Mingmei Liao, Ruijie Liu, Qi Zhang, Sai Zhang, Yi He, Jin Jin, Pengfei Zhang, Lin Zhou
Summary: This study confirmed the important role of hucMSC-derived EVs loaded with miR-223 in the treatment of MI. MiR-223 can alleviate the pathological process of MI by suppressing fibrosis and promoting angiogenesis, and promote myocardial repair by regulating the P53/S100A9 axis.
Article
Cell Biology
Santhosh Kacham, Tejal Sunil Bhure, Sindhuja D. Eswaramoorthy, Gaurav Naik, Subha Narayan Rath, Sreenivasa Rao Parcha, Sayan Basu, Virender Singh Sangwan, Sachin Shukla
Summary: Umbilical cord-derived mesenchymal stem cells promote the repair of injured corneal epithelium by stimulating the proliferation of corneal epithelial cells, potentially serving as a non-ocular source of stem cells for treating injury-induced bilateral corneal diseases.
Article
Oncology
Litao Yan, Gejun Liu, Xing Wu
Summary: This study found that exosomal lncRNA H19 derived from UMSCs acts as a competing endogenous RNA to enhance osteochondral activity in chondrocytes by sequestering miR-29b-3p. Injection of exosomes overexpressing H19 promoted sustained cartilage repair, highlighting its potential for developing strategies against cartilage defects.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Medicine, Research & Experimental
Yuanfang Zheng, Jiyuan Liu, Ping Chen, Lu Lin, Yinzhu Luo, Xiaoying Ma, Jincai Lin, Ying Shen, Liyan Zhang
Summary: In this study, it was found that exosomal miR-22-3p derived from human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) could alleviate LPS-induced ALI by regulating frizzled class receptor 6 (FZD6), reducing inflammatory reaction and oxidative stress, promoting cell proliferation activity, and inhibiting apoptosis. The results suggest the potential of UCB-MSC-exosomal miR-22-3p in preventing ALI and provide insights into ALI therapy.
Article
Biotechnology & Applied Microbiology
Kanglu Li, Guohua Yan, Hanji Huang, Mingjun Zheng, Ke Ma, Xiaofei Cui, Dejie Lu, Li Zheng, Bo Zhu, Jianwen Cheng, Jinmin Zhao
Summary: This study investigates the immunomodulatory and anti-inflammatory effects of human umbilical cord mesenchymal stem cells-derived extracellular vesicles (hUCMSCs-EVs) on osteoarthritis (OA). The results suggest that hUCMSCs-EVs promote the polarization of M2-type macrophages and the expression of IL-10, inhibit inflammation-associated factors in OA chondrocytes caused by IL-1 beta, and alleviate cartilage degradation during OA progression. The enriched miRNAs in hUCMSCs-EVs and the modulation of the PI3K-Akt signaling pathway may contribute to these therapeutic effects.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jierong Chen, Ziyue Li, Caifeng Yue, Jianhong Ma, Lixue Cao, Jiaxin Lin, Dandan Zhu, Ran An, Jinxin Lai, Yunmiao Guo, Bing Gu
Summary: This study demonstrates that exosomes released from hUC-MSCs carry miR-1827, which inhibits M2 macrophage polarization and liver metastasis in CRC by targeting SUCNR1. It also suppresses the growth, migration, and invasion of CRC cells. These findings provide a theoretical basis for using exosomes as a target therapy for CRC.
Article
Biochemistry & Molecular Biology
Kohei Homma, Eriko Toda, Hideto Osada, Norihiro Nagai, Takumi Era, Kazuo Tsubota, Hideyuki Okano, Yoko Ozawa
Summary: Mitochondrial dysfunction in MELAS patient-derived induced pluripotent stem cells resulted in impaired proliferation and survival when glycolysis was inhibited, which was rescued by taurine treatment. Metabolomic analysis revealed oxidative stress and EMT in the retinal pigment epithelium differentiated from MELAS iPSCs, both of which were alleviated by taurine. Therefore, taurine may serve as a potential therapeutic approach for mitochondrial-related retinal diseases.
Article
Biotechnology & Applied Microbiology
Mohamed A. Faynus, Jeffrey K. Bailey, Britney O. Pennington, Mika Katsura, Duncan A. Proctor, Ashley K. Yeh, Sneha Menon, Dylan G. Choi, Jane S. Lebkowski, Lincoln Johnson, Dennis O. Clegg
Summary: Dry age-related macular degeneration (AMD) is a common eye disease with no current treatment options. Using microcarrier technology for cell culture allows efficient large-scale production of therapeutic retinal pigmented epithelial cells, fulfilling the treatment demands.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Masaaki Ishida, Sunao Sugita, Kenichi Makabe, Shota Fujii, Yoko Futatsugi, Hiroyuki Kamao, Suguru Yamasaki, Noriko Sakai, Akiko Maeda, Michiko Mandai, Masayo Takahashi
Summary: Research has shown that the addition of Y-27632 in vitro can suppress apoptosis, promote cell adhesion and proliferation, and increase the survival rate of transplants. In vivo experiments with human iPS-RPE cell transplantation into monkey eyes showed no obvious retinal toxicity caused by Y-27632.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yang Wang, Xunwei Lai, Depeng Wu, Bin Liu, Nanxiang Wang, Limin Rong
Summary: The study demonstrates that exosomes transferring miR-199a-3p/145-5p into neurons in SCI rats affect TrkA ubiquitination and promote the NGF/TrkA signaling pathway, showing the potential of hUC-MSC-derived exosomes as a promising treatment strategy for SCI.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Jinying Li, Chen Qiu, Yang Wei, Weixin Yuan, Jia Liu, Wenyu Cui, Jiayi Zhou, Cong Qiu, Lihe Guo, Liquan Huang, Zhen Ge, Luyang Yu
Summary: Human amniotic epithelial stem cells can differentiate into RPE like cells with the help of trichostatin A and nicotinamide, showing potential therapeutic effects for age-related macular degeneration. Subretinal transplantation of these cells in RCS rats resulted in rescued visual function and retinal structure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Elke K. Markert, Holger Klein, Coralie Viollet, Werner Rust, Benjamin Strobel, Stefan G. Kauschke, Bar Makovoz, Heike Neubauer, Remko A. Bakker, Timothy A. Blenkinsop
Summary: This study examines the mature state and characteristics of RPE cells derived from different sources. The results show that induced pluripotent stem cell-derived RPE cells are more similar to adult primary RPE cells in terms of gene expression and characteristics, while the ARPE-19 line is different. The study also finds that iPSC-RPE cells are more sensitive to epithelial-mesenchymal transition and aggregate formation compared to ahRPE cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Byung-Chul Lee, Jin Young Lee, Juhee Kim, Je Min Yoo, Insung Kang, Jae-Jun Kim, Nari Shin, Dong Jin Kim, Soon Won Choi, Donghoon Kim, Byung Hee Hong, Kyung-Sun Kang
Article
Engineering, Biomedical
Da-Hyun Kim, Jungho Ahn, Hyun Kyoung Kang, Min-Soo Kim, Nam-Gyo Kim, Myung Geun Kook, Soon Won Choi, Noo Li Jeon, Heung-Myong Woo, Kyung-Sun Kang
Summary: Liver tissue engineering with vascular reconstruction using decellularized liver scaffolds showed enhanced liver-specific functions and perfusable vessel networks, providing a promising strategy for liver failure treatment.
Article
Chemistry, Multidisciplinary
Insung Kang, Je Min Yoo, Donghoon Kim, Juhee Kim, Myung Keun Cho, Seung-Eun Lee, Dong Jin Kim, Byung-Chul Lee, Jin Young Lee, Jae-Jun Kim, Nari Shin, Soon Won Choi, Young-Ho Lee, Han Seok Ko, Seokmin Shin, Byung Hee Hong, Kyung-Sun Kang
Summary: Graphene quantum dots (GQDs) show promise as a potential therapeutic agent for Niemann-Pick disease type C (NPC) by reducing cholesterol aggregation in lysosomes, promoting autophagy, and inhibiting Purkinje cell loss in the cerebellum. Their ability to alleviate impaired functions in NPC highlights the potential of GQDs for treating NPC and related disorders.
Article
Cell Biology
Seung-Eun Lee, Nari Shin, Myung Geun Kook, Dasom Kong, Nam Gyo Kim, Soon Won Choi, Kyung-Sun Kang
CELL DEATH & DISEASE
(2020)
Article
Cell Biology
Min Soo Kim, Da-Hyun Kim, Hyun Kyoung Kang, Myung Geun Kook, Soon Won Choi, Kyung-Sun Kang
Summary: Novel brain organoid model systems have been developed from adult dermal fibroblast-derived neural stem cells, exhibiting characteristics of the human cerebral cortex for studying neuronal proliferation and maturation. When subjected to hypoxic injury, reoxygenation restored neuronal cell proliferation but not maturation in these neural organoids, suggesting new opportunities for drug screening and personalized modeling of neurodegenerative diseases.
Article
Materials Science, Multidisciplinary
Byung-Chul Lee, Jin Young Lee, Juhee Kim, Nari Shin, Je Min Yoo, Insung Kang, Jae-Jun Kim, Seung-Eun Lee, Donghoon Kim, Soon Won Choi, Byung Hee Hong, Kyung-Sun Kang
Summary: Graphene-based nanomaterials, particularly graphene quantum dots (GQDs), have shown promising potential as alternative drugs for rare diseases due to their high biocompatibility and low toxicity. Oral administration of GQDs was demonstrated to effectively ameliorate colitis symptoms without harmful effects, highlighting its potential as a microbiome-friendly treatment option for colitis.
Article
Biochemistry & Molecular Biology
Se-Hwa Jung, Ji-Eun You, Soon-Won Choi, Kyung-Sun Kang, Je-Yeol Cho, Jungmook Lyu, Pyung-Hwan Kim
Summary: The study investigated the biological function of the PKD1 gene in umbilical cord blood-derived mesenchymal stem cells (UCB-MSC), finding that it can enhance differentiation ability and maintain long-term stemness, providing new possibilities for stem cell therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Nari Shin, Namhee Jung, Seung-Eun Lee, Dasom Kong, Nam Gyo Kim, Myung Geun Kook, Hwanhee Park, Soon Won Choi, Seunghee Lee, Kyung-Sun Kang
Summary: This study revealed that coadministration of pimecrolimus interfered with the therapeutic efficacy of hUCB-MSCs in atopic dermatitis, highlighting the interaction of hMSCs with other drugs in cell therapy for AD.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Hyun Kyoung Kang, Madina Sarsenova, Da-Hyun Kim, Min Soo Kim, Jin Young Lee, Eun-Ah Sung, Myung Geun Kook, Nam Gyo Kim, Soon Won Choi, Vyacheslav Ogay, Kyung-Sun Kang
Summary: The incorporation of spinning condition and continuous media stimuli in bioprinted hepatic constructs enhances HepG2 cell proliferation and functionality, leading to improved drug effect prediction in vitro. HepG2 cells under spinning conditions exhibit increased sensitivity to hepatotoxicity and enhanced drug-induced signals and responses, making them a valuable tool for toxicological evaluation.
Article
Biotechnology & Applied Microbiology
Nari Shin, Youngtaek Kim, Jihoon Ko, Soon Won Choi, Sujin Hyung, Seung-Eun Lee, Seunghyuk Park, Jiyoung Song, Noo Li Jeon, Kyung-Sun Kang
Summary: A three-dimensional vascularized spheroid cell model was developed in this study, demonstrating the significant role of vascular networks in enhancing differentiation and reducing apoptosis in neural spheroids. This platform enables further exploration of the functional and structural interactions between blood vessels and neural spheroids, with the ultimate goal of simulating brain development and diseases.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Cell Biology
Seung-Eun Lee, Daekee Kwon, Nari Shin, Dasom Kong, Nam Gyo Kim, Hee-Yeong Kim, Min-Ji Kim, Soon Won Choi, Kyung-Sun Kang
Summary: Mitochondrial dysfunction and failure of mitophagy in familial Alzheimer's disease (fAD) are associated with the accumulation of amyloid precursor protein-derived C-terminal fragments (APP-CTFs). This study demonstrates that the accumulation of APP-CTFs within mitochondrial domains is both a cause and consequence of mitochondrial dysfunction, leading to impaired mitophagy function.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Seung-Eun Lee, Hanul Choi, Nari Shin, Dasom Kong, Nam Gyo Kim, Hee-Yeong Kim, Min-Ji Kim, Soon Won Choi, Young Bong Kim, Kyung-Sun Kang
Summary: Alzheimer's disease is a progressive neurodegenerative disease with unclear mechanisms. Viral infection is one of the risk factors for this disease, triggering the expression of AD-related features in the brain. Inhibitors of PERK can alleviate the pathological features induced by infection.
CELL DEATH DISCOVERY
(2022)
Article
Materials Science, Biomaterials
Hyungwon Moon, Kihwan Hwang, Kyung Mi Nam, Yoon-Seok Kim, Min Jung Ko, Hyun Ryoung Kim, Hak Jong Lee, Mi Jeong Kim, Tae Ho Kim, Kyung-Sun Kang, Nam Gyo Kim, Soon Won Choi, Chae-Yong Kim
Summary: This study developed sonosensitive liposome encapsulating doxorubicin (DOX) combined with focused ultrasound (FUS) and microbubbles to enhance the penetration of glioblastoma, overcoming the challenge posed by the blood-brain barrier (BBB).
BIOMATERIALS ADVANCES
(2022)
Article
Cell & Tissue Engineering
Myung Geun Kook, Seung-Eun Lee, Nari Shin, Dasom Kong, Da-Hyun Kim, Min-Soo Kim, Hyun Kyoung Kang, Soon Won Choi, Kyung-Sun Kang
Summary: This study developed vascularized cerebral organoids through the assembly of brain organoids with vascular spheroids, providing a model for studying human cortical development and an opportunity to explore neurological diseases.
INTERNATIONAL JOURNAL OF STEM CELLS
(2022)
Article
Veterinary Sciences
Soon-Won Choi, Yoon-Hwan Kim, Min Soo Kang, Yunho Jeong, Jin-Ok Ahn, Jung Hoon Choi, Jin-Young Chung
Summary: This study compared cytokine levels between healthy dogs and dogs suspected of acute pancreatitis, finding significant increases in IL-8, MCP-1, RAGE, and SCF in dogs with suspected acute pancreatitis, while IL-2, IL-6, IL-10, GM-CSF, and TNF-alpha showed no significant differences.
VETERINARY SCIENCES
(2021)