Article
Cell Biology
Lothar Baltruschat, Luigi Prisco, Philipp Ranft, J. Scott Lauritzen, Andre Fiala, Davi D. Bock, Gaia Tavosanis
Summary: This study focuses on the calyx of the Drosophila mushroom body and shows that appetitive long-term memory leads to an increase in functional microglomeruli responding to the conditioned odor.
Article
Genetics & Heredity
Runa Hamid, Hitesh Sonaram Sant, Mrunal Nagaraj Kulkarni
Summary: The study demonstrates the crucial role of Choline Transporter (ChT) in habituation in Drosophila, showing that it regulates habituation and incoming stimuli through interactions between excitatory and inhibitory neurons at multiple circuit loci. The lack of ChT in MBs may lead to characteristics similar to Autism spectrum disorders (ASD), suggesting a potential link between ChT dysfunction and neuropsychiatric disorders.
Article
Biochemistry & Molecular Biology
Ziyu Lu, Melissa Zhang, Jasper Lee, Andras Sziraki, Sonya Anderson, Zehao Zhang, Zihan Xu, Weirong Jiang, Shaoyu Ge, Peter T. Nelson, Wei Zhou, Junyue Cao
Summary: Progenitor cells in the aged brain play a critical role in maintaining organismal homeostasis, but their diversity and dynamics have been overlooked. A new single-cell genomic method called TrackerSci was introduced to characterize the transcriptome and chromatin landscape of proliferating progenitor cells in vivo. The study revealed diverse progenitor cell types in the brain and identified aging-associated shifts in cell-type-specific proliferation and differentiation. Conserved genetic signatures across species were also discovered in the progenitor cells of the aged human brain, along with region-specific cellular dynamics.
Article
Developmental Biology
Sophie E. Keegan, Julie Haskins, Andrew J. Simmonds, Sarah C. Hughes
Summary: Coordinated spatio-temporal regulation of the determination and differentiation of neural stem cells is crucial for brain development. Snr1, an ATP-dependent chromatin remodelling protein, plays a key role in the transition of neuroepithelial cells to neural stem cells and the subsequent differentiation of neural stem cells. Loss of Snr1 leads to premature neural stem cell formation and inappropriate persistence of neural stem cells into adulthood. Snr1 is associated with actively transcribed chromatin region and likely regulates chromatin state for proper brain development.
Article
Biochemistry & Molecular Biology
Yoko Suzuki-Horiuchi, Henning Schmitz, Carlotta Barlassina, David Eccles, Martina Sinn, Claudia Ortmeier, Soren Moritz, Luca Gentile
Summary: Regeneration is common in the animal kingdom, but humans have limited regenerative abilities. Advances in bioengineering, stem cells, and regenerative biology have led to the development of regenerative medical treatments. Planarians, with their unique regenerative capabilities, serve as an ideal model for studying transcriptional networks involved in regeneration.
Article
Plant Sciences
Yixiong Bai, Xiaohong Zhao, Xiaohua Yao, Youhua Yao, Xin Li, Lu Hou, Likun An, Kunlun Wu, Zhonghua Wang
Summary: In this study, we analyzed the gene expression of lodging-resistant and lodging-sensitive varieties of hulless barley and found that the lodging-resistant variety had significantly higher lignin concentration, lignin monomer contents, and neutral detergent fibre compared to the lodging-sensitive variety. Through transcriptome sequencing analysis, we identified 72 differentially expressed genes, including 17 genes related to lignin, cellulose, and hemicellulose synthesis or regulation, providing a basis for further exploring the molecular mechanism of stem lodging resistance in hulless barley and valuable gene resources for stem lodging resistance molecular breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Cell Biology
Leo Machado, Frederic Relaix, Philippos Mourikis
Summary: The rapid advancement of single-cell RNA-sequencing technology has made it possible to generate comprehensive transcriptional maps of nearly all cells in multicellular tissues. Key elements such as scalability, spatial information, and accuracy of individual cell transcriptomes are critical in this process. Traditional cell-isolation protocols and new methods have been discussed in terms of capturing the transcriptional signatures of cells in their native tissue context, revealing new insights into biological processes.
TRENDS IN CELL BIOLOGY
(2021)
Article
Neurosciences
Adelya Albertovna Galiakberova, Olga Igorevna Brovkina, Nikolay Vitalyevich Kondratyev, Alexander Sergeevich Artyuhov, Ekaterina Dmitrievna Momotyuk, Olga Nikolaevna Kulmukhametova, Alexey Aleksandrovich Lagunin, Boris Vladimirovich Shilov, Anton Dmitrievich Zadorozhny, Igor Sergeevitch Zakharov, Larisa Sergeevna Okorokova, Vera Evgenievna Golimbet, Erdem Bairovich Dashinimaev
Summary: Culturing human neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSC) is a promising area of research, but the stability of these cells during long-term culturing remains a challenge. This study investigated the spontaneous differentiation profile of iPSC-derived NSCs during long-term cultivation. Results showed that different NSC lines generate significantly different spectrums of differentiated neural cells, which can also change significantly during long-term cultivation. These findings have important implications for the development of optimal NSCs culturing protocols and highlight the need for further investigation into the factors influencing the stability of these cells in vitro.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yan-Rong Feng, Sayed Haidar Abbas Raza, Cheng-Cheng Liang, Xiao-Yu Wang, Jian-Fang Wang, Wen-Zheng Zhang, Linsen Zan
Summary: CREB1 plays an important role in Qinchuan bovine skeletal myoblasts, promoting cell proliferation and differentiation by mediating the transcription of CCNA2 and MyoG. It is involved in cell proliferation, myogenic differentiation, and skeletal muscle repair pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Zachary Johnson, Yu Lin, Hehuang Xie, Xiguang Xu
Summary: Post-transcriptional RNA modifications play a crucial role in neuronal differentiation and synapse development in the mammalian brain. In this study, the researchers characterized the methylation profiles of mRNA in the developing brain, identifying m5C sites that are consistently methylated across different conditions. They found that the majority of these methylated sites are hypermethylated in neurons compared to NSCs, and are associated with genes involved in positive transcriptional regulation and axon extension. Furthermore, there were significant changes in RNA cytosine methylation and the expression of related genes during early postnatal brain development, with differentially methylated transcripts enriched for genes regulating synaptic plasticity.
Article
Virology
Alejandro Casco, Akansha Gupta, Mitchell Hayes, Reza Djavadian, Makoto Ohashi, Eric Johannsen
Summary: Herpesviruses employ extensive bidirectional transcription of overlapping genes to overcome length constraints. Traditional methods struggle to accurately measure this complexity. The study introduces UCDS and UTS methods to estimate gene abundance from RNA-seq data, with UTS showing the highest accuracy and compatibility with overlapping genes.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tian Lan, Gyorgy Hutvagner, Xuan Zhang, Tao Liu, Limsoon Wong, Jinyan Li
Summary: This article introduces a density-based trajectory inference method that can accurately construct various complex cell differentiation trajectories and validates the practicality of the method on real data.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Entomology
Gabriele Rondoni, Alessandro Roman, Camille Meslin, Nicolas Montagne, Eric Conti, Emmanuelle Jacquin-Joly
Summary: Limited knowledge exists regarding the mechanisms underlying the sense of smell and taste in the predatory harlequin ladybird Harmonia axyridis. This study describes the first chemosensory gene repertoire expressed in the antennae of male and female H. axyridis, paving the way for future studies aiming at increasing the efficacy of biological control or reducing its populations in invasive areas.
Article
Fisheries
Baohua Duan, Tongxu Kang, Chen Zhang, Haifu Wan, Shumei Mu, Yueqiang Guan, Weibiao Liu, Zejian Li, Yang Tian, Yuqin Ren, Xianjiang Kang
Summary: This study combined BSA-seq and RNA-seq to identify potential candidate genes related to the body weight of the swimming crab. BSA-seq successfully identified five candidate intervals, while RNA-seq analysis yielded 1581 differentially expressed genes. Enrichment analysis showed that these genes were involved in proteolysis, nucleus, and molecular function. Eight critical differentially expressed genes were also identified.
AQUACULTURE REPORTS
(2023)
Article
Engineering, Biomedical
Jong-Tae Kim, Sung Min Cho, Dong Hyuk Youn, Eun Pyo Hong, Chan Hum Park, Younghyurk Lee, Harry Jung, Jin Pyeong Jeon
Summary: This study evaluated the therapeutic effect of a new delivery method of mouse neural stem cell (mNSC) spheroids using a hydrogel, in terms of improvement in damaged cortical lesions and cognitive impairment after mild traumatic brain injury (TBI). The results showed that transplantation of hydrogel-based mNSC spheroids improved cognitive function through reconstruction of the damaged cortex.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Qingzhong Ren, Takeshi Awasaki, Yu-Fen Huang, Zhiyong Liu, Tzumin Lee
Article
Multidisciplinary Sciences
Lisha Shao, Mathias Saver, Phuong Chung, Qingzhong Ren, Tzumin Lee, Clement F. Kent, Ulrike Heberlein
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Developmental Biology
Qingzhong Ren, Takeshi Awasaki, Yu-Chun Wang, Yu-Fen Huang, Tzumin Lee
Article
Developmental Biology
Yu-Chun Wang, Jacob S. Yang, Rebecca Johnston, Qingzhong Ren, Ying-Jou Lee, Haojiang Luan, Thomas Brody, Ward F. Odenwald, Tzumin Lee
Article
Neurosciences
Qingzhong Ren, Hao Li, Yanying Wu, Jing Ren, Aike Guo
JOURNAL OF NEUROSCIENCE
(2012)
Article
Neurosciences
Xiaonan Zhang, Qingzhong Ren, Aike Guo
JOURNAL OF NEUROSCIENCE
(2013)
Article
Neurosciences
Yanying Wu, Qingzhong Ren, Hao Li, Aike Guo
Article
Multidisciplinary Sciences
Jing Ren, Jinghan Sun, Yunpeng Zhang, Tong Liu, Qingzhong Ren, Yan Li, Aike Guo
Article
Developmental Biology
Hui-Min Chen, Xiaohao Yao, Qingzhong Ren, Chuan-Chie Chang, Ling-Yu Liu, Rosa Linda Miyares, Tzumin Lee
Article
Biology
Ying-Jou Lee, Ching-Po Yang, Rosa L. Miyares, Yu-Fen Huang, Yisheng He, Qingzhong Ren, Hui-Min Chen, Takashi Kawase, Masayoshi Ito, Hideo Otsuna, Ken Sugino, Yoshi Aso, Kei Ito, Tzumin Lee
Article
Biochemistry & Molecular Biology
Qingzhong Ren, Ching-Po Yang, Zhiyong Liu, Ken Sugino, Kent Mok, Yisheng He, Masayoshi Ito, Aljoscha Nern, Hideo Otsuna, Tzumin Lee