期刊
JOURNAL OF BIOPHOTONICS
卷 11, 期 4, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201700207
关键词
cancer cell uptake; diatomite; drug delivery; nanomaterials; porous biosilica nanoparticles; Raman imaging
资金
- Associazione Italiana per la Ricerca sul Cancro [1154]
- Ministero dell'Istruzione, dell'Universita e della Ricerca [FIRB RBFR12WAPY]
- Regione Campania
Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non-toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution of functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up to 72hours are investigated by Raman imaging. The label-free Raman results are compared with confocal fluorescence microscopy and photoluminescence (PL) data. Raman bands specifically assigned to DNPs and cellular components provide evidence that the nanovectors are internalized and co-localize with lipid environments. A considerable DNPs uptake in cells is observed within 6hours, with equilibrium being achieved after 18hours. The obtained data show the presence of DNPs up to 72hours, without damage to cell viability or morphology. The PL measurements performed on DNPs not penetrating the cells at different incubation times are strongly correlated with the results obtained by Raman imaging and confocal microscopy analyses.
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