Review
Biochemistry & Molecular Biology
Ashley J. Ovens, John W. Scott, Christopher G. Langendorf, Bruce E. Kemp, Jonathan S. Oakhill, William J. Smiles
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in adaptive responses to exercise, and its post-translational modifications extensively influence its activity, subcellular localization, nutrient interactions, and protein stability. Areas for further research include novel regulatory modifications of AMPK and improving characterization of these modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Malgorzata Tokarska-Schlattner, Laurence Kay, Pascale Perret, Raffaella Isola, Stephane Attia, Frederic Lamarche, Cindy Tellier, Cecile Cottet-Rousselle, Amjad Uneisi, Isabelle Hininger-Favier, Marc Foretz, Herve Dubouchaud, Catherine Ghezzi, Christian Zuppinger, Benoit Viollet, Uwe Schlattner
Summary: AMPK, a key regulator of energy homeostasis, plays important roles in maintaining cardiac function under increased workload conditions. Deletion of AMPK genes in cardiomyocytes did not affect heart function under physiological conditions, but led to alterations in response to increased workload. AMPK deletion also resulted in decreased basal metabolic rate and locomotor activity, along with changes in cardiac mitochondrial function and bioenergetics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Iskander Madhi, Ji-Hee Kim, Ji Eun Shin, Younghee Kim
Summary: Ginsenoside Re (G-Re) inhibits the production of inflammatory mediators in microglial cells and protects hippocampal cells from inflammatory and neurotoxic factors. This suggests G-Re may be a potential treatment option for neuroinflammatory disorders and various neurodegenerative diseases.
MOLECULAR MEDICINE REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Hiroshi Tokumitsu, Hiroyuki Sakagami
Summary: CaMKK acts as the activating kinase for multiple downstream kinases, playing important roles in various Ca2+-dependent pathways as well as pathophysiological pathways. Its molecular mechanism and pharmacological inhibitors are areas of focus in research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Fiona M. Russell, David Grahame Hardie
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in regulating cellular energy balance by promoting ATP production and inhibiting cell growth. It may restrain aberrant growth before tumorigenesis, but support the survival of cancer cells once cancer has arisen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yu-Hsu Chen, Kuang-Kai Hsueh, Pei-Wen Chu, Shau-Kwaun Chen
Summary: AMP-activated protein kinase (AMPK) is a critical metabolic regulator that affects immune cell functions such as macrophages and osteoclasts. AMPK plays a key role in promoting anti-inflammatory responses in macrophages and negatively regulating osteoclastogenesis.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yusei Fukumoto, Yuhei Harada, Satomi Ohtsuka, Naoki Kanayama, Masaki Magari, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu
Summary: Ca2+/calmodulin-dependent protein kinase kinases (CaMKK alpha and beta) form oligomers in cells, with CaMKK alpha self-associating to create a catalytically active complex that is crucial for efficient intracellular signaling activation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Marie-Sophie Nguyen-Tu, Joseph Harris, Aida Martinez-Sanchez, Pauline Chabosseau, Ming Hu, Eleni Georgiadou, Alice Pollard, Pablo Otero, Livia Lopez-Noriega, Isabelle Leclerc, Kei Sakamoto, Dieter Schmoll, David M. Smith, David Carling, Guy A. Rutter
Summary: The role of AMP-activated protein kinase (AMPK) in the control of insulin secretion is still debatable. AMPK activation exerts complex, time-dependent effects on insulin secretion. These observations should inform the design and future clinical use of AMPK modulators.
Article
Neurosciences
Xin Wang, Xueyan Zhou, Beth Uberseder, Jingyun Lee, Caitlin S. Latimer, Cristina M. Furdui, C. Dirk Keene, Thomas J. Montine, Thomas C. Register, Suzanne Craft, Carol A. Shively, Tao Ma
Summary: The study on NHP model demonstrated an increased activity of AMPK alpha 2 in AD-like neuropathology, unrelated to AMPK alpha 1; CSF Abeta(42) showed a negative correlation with cerebral amyloid plaque density; A beta plaque burden correlated with brain A beta measurement levels.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Pharmacology & Pharmacy
Gang Zhong, Huiping Long, Fei Chen, Yin Yu
Summary: In this study, oxoglaucine was found to attenuate progression of deterioration and pathological changes in osteoarthritis by activating autophagy and inhibiting calcium influx. It suppressed expression of proinflammatory and apoptosis-related proteins, prevented matrix degradation in OA chondrocytes, and blocked Ca2+ influx while up-regulating autophagic markers. Oxoglaucine shows potential as a candidate drug for OA treatment.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Xijin Zhu, Xiangying Kong, Lei Zang, Nan Sun, Qunli Yu, Ling Han
Summary: This study investigated the effects of oxidative stress on early postmortem glycolysis and water-holding capacity (WHC) in yak meat. The results showed that oxidative stress promoted AMPK activation and glycolysis, reducing the WHC of yak meat. These effects were likely mediated by the CaMKK beta/AMPK signaling pathway.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Cell Biology
Li Zhang, Xinyang Yu, Yue Wu, Huijia Fu, Ping Xu, Yangxi Zheng, Li Wen, Xiaotao Yang, Fumei Zhang, Mingyu Hu, Hao Wang, Xiyao Liu, Juan Qiao, Chuan Peng, Rufei Gao, Richard Saffery, Yong Fu, Hongbo Qi, Chao Tong, Mark D. Kilby, Philip N. Baker
Summary: The study found that GDM is associated with impaired glycolysis, AMPK activity, GLUT3 expression, and cell survival in the placenta. Hyperglycemia induced similar changes in trophoblasts, and these changes were rescued by AMPK activation. AMPK activation upregulates trophoblast glucose uptake by stimulating GLUT3 translocation, which is beneficial for viability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Bin Wang, Xiaoying Zhou, Scott A. Gerber, Jennifer J. Loros, Jay C. Dunlap
Summary: The study reveals that changes in intracellular calcium levels in the Neurospora clock affect the phosphorylation of core clock components, leading to a shortened circadian period length.
Article
Dentistry, Oral Surgery & Medicine
Oliver Pieles, Maria Hartmann, Christian Morsczeck
Summary: This study found that AMPK and autophagy markers are regulated during osteogenic differentiation of Dental Follicle Cells (DFCs). Activation of AMPK inhibits ALP activity and other osteogenic markers, while AMPK inhibition and autophagy activation increased expression of some osteogenic markers. Both activation and inhibition of AMPK significantly inhibited biomineralization of DFCs in long-term cultures. Autophagy inhibition enhanced biomineralization while autophagy activation diminished mineralization capability of DFCs. AMPK plays a role in regulating osteogenic differentiation, indirectly affecting biomineralization partly via autophagy.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dawei Yang, Rong Rong, Rongliang Yang, Mengling You, Mengxiao Wang, Haibo Li, Dan Ji
Summary: This study elucidated the molecular mechanism of retinal cell damage caused by blue light, revealing that the CaMKII-Drp1 pathway plays a significant role in mediating this damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Maho Yamamoto, Rina Kondo, Haruka Hozumi, Seita Doi, Miwako Denda, Masaki Magari, Naoki Kanayama, Naoya Hatano, Ryo Morishita, Hiroshi Tokumitsu
Summary: This study identified HMG20A as a novel binding partner of S100A6, showing Ca2+-dependent interaction in various experimental settings. The specific C-terminal region of HMG20A (residues 311-342) was highlighted as essential for the interaction with S100A6.
Article
Cell Biology
Toshio Kitazawa, Toshiyasu Matsui, Shuichi Katsuki, Akira Goto, Kai Akagi, Naoya Hatano, Hiroshi Tokumitsu, Kosuke Takeya, Masumi Eto
Summary: In this study, it was found that Ca2+-induced Ca2+ desensitization of LC20 phosphorylation in phasic smooth muscles is caused by MLCK inactivation. Inhibition of CaMKK beta attenuated both the phasic response of contraction and MLCK phosphorylation, while inhibition of protein phosphatase 2A amplified the phasic response.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Seita Doi, Naoki Fujioka, Satomi Ohtsuka, Rina Kondo, Maho Yamamoto, Miwako Denda, Masaki Magari, Naoki Kanayama, Naoya Hatano, Ryo Morishita, Takafumi Hasegawa, Hiroshi Tokumitsu
Summary: The study identified TPPP as a novel binding partner for S100A2 and confirmed their interaction through various experimental methods. Additionally, S100A6 and S100B were also found to potentially interact with TPPP and regulate its physiological function.
Article
Biochemistry & Molecular Biology
Yusei Fukumoto, Yuhei Harada, Satomi Ohtsuka, Naoki Kanayama, Masaki Magari, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu
Summary: Ca2+/calmodulin-dependent protein kinase kinases (CaMKK alpha and beta) form oligomers in cells, with CaMKK alpha self-associating to create a catalytically active complex that is crucial for efficient intracellular signaling activation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Satomi Ohtsuka, Taisei Okumura, Yuna Tabuchi, Tomoyuki Miyagawa, Naoki Kanayama, Masaki Magari, Naoya Hatano, Hiroyuki Sakagami, Futoshi Suizu, Teruhiko Ishikawa, Hiroshi Tokumitsu
Summary: This study developed an inhibitor, TIM-063, that interacts with CaMKK, and the interaction is dependent on the concentration of intracellular Ca2+.
Article
Biochemistry & Molecular Biology
Riku Kaneshige, Satomi Ohtsuka, Yuhei Harada, Issei Kawamata, Masaki Magari, Naoki Kanayama, Naoya Hatano, Hiroyuki Sakagami, Hiroshi Tokumitsu
Summary: This study reveals that the RP-domain is required for CaMKK isoforms to recognize and phosphorylate downstream kinases, potentially providing a promising target for specific CaMKK inhibitors.
Article
Biochemistry & Molecular Biology
Masaki Magari, Miku Nishioka, Tomomi Hari, Sayaka Ogawa, Kaho Takahashi, Naoya Hatano, Naoki Kanayama, Junichiro Futami, Hiroshi Tokumitsu
Summary: The homophilic interaction of SLAMF8 promotes FDMC differentiation and SLAMF8 might act as a novel regulator of GC responses by regulating FDMC differentiation.
Article
Biochemistry & Molecular Biology
Kento Nlandu Nakamura, Haruki Yamauchi, Hiroyuki Mima, Yerun Chen, Satomi Ohtsuka, Masaki Magari, Ryo Morishita, Hiroshi Tokumitsu
Summary: In this study, a fusion molecule of AirID and rat CaM (AirID-CaM) was used to examine the physical interactions between CaM and its target proteins. The results showed that AirID-CaM can catalyze the Ca2+-dependent biotinylation of target proteins, indicating interaction-dependent biotinylation. It was also observed that AirID-CaM can detect CaM/target interactions with relatively high sensitivity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)