Article
Oncology
Simrit Safarulla, Ankit Madan, Fei Xing, Arvind Chandrasekaran
Summary: This study reveals the mechanisms by which CXCR2 affects neutrophils in brain metastasis of breast cancer. Activation of CXCR2 receptors by brain metastatic tumors promotes neutrophil migration to tumor sites and formation of NETs, thereby facilitating tumor progression. Inhibiting CXCR2 and NETs formation can limit the interaction between neutrophils and tumors, slowing down the development of brain metastatic breast cancer.
Article
Multidisciplinary Sciences
Boris Simonetti, James L. Daly, Lorena Simon-Gracia, Katja Klein, Saroja Weeratunga, Carlos Anton-Plagaro, Allan Tobi, Lorna Hodgson, Philip A. Lewis, Kate J. Heesom, Deborah K. Shoemark, Andrew D. Davidson, Brett M. Collins, Tambet Teesalu, Yohei Yamauchi, Peter J. Cullen
Summary: Endosomal sorting is important for maintaining cellular homeostasis. The study identified NRP1 as a cargo protein bound and trafficked by the ESCPE-1 complex, which may play a role in intracellular membrane trafficking of NRP1-interacting viruses such as SARS-CoV-2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Noura Al-Zeheimi, Yan Gao, Peter A. A. Greer, Sirin A. A. Adham
Summary: Breast cancer metastasis is a leading cause of female mortality, and Neuropilin-1 (NRP-1) plays a ligand-dependent role in breast cancer. While clinical studies have shown a link between NRP-1 and metastatic disease, its exact role in breast cancer metastasis remains uncertain. In this study, the NRP-1 gene was knocked out in MDA-MB-231 breast cancer cells using CRISPR-Cas9, and the effects on metastasis were investigated using a mouse engraftment model. Knockout cells showed reduced metastasis to the lungs, and transcriptome analysis revealed altered pathways and changes in genes associated with metastasis. These findings suggest a functional role for NRP-1 in breast cancer metastasis and support further exploration of NRP-1 and the identified genes as potential targets for treating metastatic breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jessica Basso, Jimin Zhang, Daniel Lasala, Sasha J. Rose, Kuan-Ju Chen, David Cipolla
Summary: This study compares two methods for extracting neutrophil serine proteases (NSPs) and finds that the NP40 cell pellet extraction method is more effective in extracting active NSPs compared to the zymosan stimulation method.
Article
Biotechnology & Applied Microbiology
Brandon Cieniewicz, Ankit Bhatta, Damoun Torabi, Priya Baichoo, Mike Saxton, Alexander Arballo, Linh Nguyen, Sunil Thomas, Harini Kethar, Phanidhar Kukutla, Omolola Shoaga, Bi Yu, Zhuo Yang, Maria Fate, Edson Oliveira, Hongxiu Ning, Lawrence Corey, Daniel Corey
Summary: In order to improve cancer cell removal, a novel cell engineering and therapeutic strategy was developed, combining phagocytic clearance and antigen presentation activity into T cells. This was achieved by engineering a chimeric receptor that enhances phagocytosis and T cell cytotoxic function. The engineered T cells demonstrated target-dependent phagocytic function and induced transcriptional signatures of key regulators responsible for phagocytic recognition and uptake. In pre-clinical models, these T cells were able to elicit immune responses and showed potential for achieving tumor control.
Article
Cell Biology
Yutaro Mori, Amrendra K. Ajay, Jae-Hyung Chang, Shan Mou, Huiping Zhao, Seiji Kishi, Jiahua Li, Craig R. Brooks, Sheng Xiao, Heung-Myong Woo, Venkata S. Sabbisetti, Suetonia C. Palmer, Pierre Galichon, Li Li, Joel M. Henderson, Vijay K. Kuchroo, Julie Hawkins, Takaharu Ichimura, Joseph Bonventre
Summary: Tubulointerstitial abnormalities can predict the progression of diabetic kidney disease, with KIM-1 playing a crucial role in this process. Targeting KIM-1 could be a potential therapeutic approach to alleviate renal damage.
Article
Pharmacology & Pharmacy
Nidhi Sharma, Taslim B. Shaikh, Abhisheik Eedara, Madhusudhana Kuncha, Ramakrishna Sistla, Sai Balaji Andugulapati
Summary: Dehydrozingerone (DHZ) attenuates liver fibrosis by reducing fibrotic markers and their activities through modulation of the MAPK pathway. These findings suggest that DHZ may be a potential therapeutic molecule for treating liver fibrosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Mauricio Campos-Mora, Javiera De Solminihac, Carolina Rojas, Cristina Padilla, Monica Kurte, Rodrigo Pacheco, Thilo Kaehne, Ursula Wyneken, Karina Pino-Lagos
Summary: This study demonstrates that Treg cells secrete sEV containing Nrp1, which play a crucial role in regulating the phenotype and proliferation of effector CD4+ T cells, as well as promoting skin transplantation tolerance.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Oncology
Tilman D. Rachner, Sabine Kasimir-Bauer, Andy Goebel, Kati Erdmann, Oliver Hoffmann, Martina Rauner, Lorenz C. Hofbauer, Rainer Kimmig, Ann-Kathrin Bittner
Summary: sNRP-1 levels are weakly correlated with age in breast cancer patients, with higher levels in peri- and postmenopausal patients compared to premenopausal patients; low sNRP-1 levels are associated with a significant survival benefit.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiao Liu, Kevin Vanvarenberg, Kobenan Guy Wilfried Kouassi, Sohaib Mahri, Rita Vanbever
Summary: In this study, a PEGylated version of AAT was produced and characterized in vitro, showing potential for prolonged body residence time and improved therapeutic efficacy via intravenous and inhalation routes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Yaqiong Yu, Yoko Uchida-Fukuhara, Yao Weng, Yuhan He, Mika Ikegame, Ziyi Wang, Kaya Yoshida, Hirohiko Okamura, Lihong Qiu
Summary: In this study, the roles of NRP1 and GAG-modifiable NRP1 in adipogenesis were explored in C3H10T1/2 cells. The expression of full-length NRP1 and GAG-modifiable NRP1 increased during adipogenic differentiation, and NRP1 knockdown repressed adipogenesis. JIP4 was found to be involved in adipogenesis by interacting with NRP1. Moreover, the non-GAG-modifiable NRP1 mutant greatly promoted adipogenic differentiation by activating the Akt and ERK1/2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Austin H. Williams, Adrien M. Hebert, Robert C. Boehm, Mary E. Huddleston, Meghan R. Jenkins, Orlin D. Velev, M. Tyler Nelson
Summary: Highly porous and ultrasoft polymeric mats mimicking human tissues were formed from novel polyurethane soft dendritic colloids, which have a unique fibrillar morphology. The mechanical properties of the mats can be tuned for physiological relevance, and they were found to support cellular growth and viability, with implications for nanoparticle uptake and cytokine secretion.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Ik Soo Lee, Yu-Ri Lee, Jea Heon Sim, Ki Mo Kim, Young Sook Kim
Summary: 1-O-acetylbritannilactone (ABL), a natural sesquiterpene lactone, was isolated from the flowers of Inula britannica during a search for natural inflammatory inhibitors. ABL exhibited significant inhibitory effects on human neutrophil elastase (HNE) and the production of nitric oxide and prostaglandin E-2. The anti-inflammatory effects of ABL were confirmed using a zebrafish larval model.
Article
Cell Biology
Chinchu Jagadan Ushakumari, Qiong L. Zhou, Yu-Hua Wang, Sijia Na, Michael C. Rigor, Cindy Y. Zhou, Max K. Kroll, Benjamin D. Lin, Zhen Y. Jiang
Summary: In this study, it was found that neutrophil elastase (NE) activates protease-activated receptor-2 (PAR2) signaling pathway and promotes changes in actomyosin and VE-cadherin, leading to increased vascular permeability and leukocyte infiltration in obesity-related inflammation. Inhibition of NE may be a potential approach to mitigate vascular injury and leukocyte infiltration in obesity-related systemic inflammation.
Article
Spectroscopy
Qi Sun, Xiang Li, Yun Guo, Yuan Qiu, Xiaogang Luo, Genyan Liu, Yunfeng Han
Summary: Researchers have designed a novel fluorescent probe HNCOU-NE for visualizing NE activity in living cells and zebrafish, which can be used for qualitative and quantitative detection of NE activity in vitro and in vivo.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Oncology
Cai Xu, Jian-Yue Jin, Ming Zhang, Amy Liu, Jun Wang, Radhe Mohan, Fengming (Spring) Kong, Steven H. Lin
RADIOTHERAPY AND ONCOLOGY
(2020)
Editorial Material
Biochemistry & Molecular Biology
Satyendra Chandra Tripathi, Mohit Kumar Jolly, Sendurai A. Mani, Herbert Levine
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Oncology
Ichidai Tanaka, Delphine Dayde, Mei Chee Tai, Haruki Mori, Luisa M. Solis, Satyendra C. Tripathi, Johannes F. Fahrmann, Nese Unver, Gargy Parhy, Rekha Jain, Edwin R. Parra, Yoshiko Murakami, Clemente Aguilar-Bonavides, Barbara Mino, Muge Celiktas, Dilsher Dhillon, Julian Phillip Casabar, Masahiro Nakatochi, Francesco Stingo, Veera Baladandayuthapani, Hong Wang, Hiroyuki Katayama, Jennifer B. Dennison, Philip L. Lorenzi, Kim-Anh Do, Junya Fujimoto, Carmen Behrens, Edwin J. Ostrin, Jaime Rodriguez-Canales, Tetsunari Hase, Takayuki Fukui, Taisuke Kajino, Seiichi Kato, Yasushi Yatabe, Waki Hosoda, Koji Kawaguchi, Kohei Yokoi, Toyofumi F. Chen-Yoshikawa, Yoshinori Hasegawa, Adi F. Gazdar, Ignacio I. Wistuba, Samir Hanash, Ayumu Taguchi
Summary: SRGN is markedly overexpressed in TTF-1-negative LUAD cell lines, associated with poor clinical outcome, and influences PD-L1 expression and immune cell infiltration. SRGN expression is regulated by DNA demethylation and methionine metabolism.
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2022)
Article
Multidisciplinary Sciences
Beatrice Biferali, Valeria Bianconi, Daniel Fernandez Perez, Sophie Poehle Kronawitter, Fabrizia Marullo, Roberta Maggio, Tiziana Santini, Federica Polverino, Stefano Biagioni, Vincenzo Summa, Carlo Toniatti, Diego Pasini, Sigmar Stricker, Romano Di Fabio, Fulvio Chiacchiera, Giovanna Peruzzi, Chiara Mozzetta
Summary: The study identified Prdm16 as a nuclear envelope protein that anchors H3K9-methylated chromatin in a cell-specific manner and regulates developmental capacities of fibro-adipogenic progenitors (FAPs). Prdm16 orchestrates lamina-associated domain organization and heterochromatin sequestration at the nuclear periphery, affecting gene expression and cell fate in FAPs.
Article
Oncology
Arvind Pandey, Satyendra C. Tripathi, Junhua Mai, Samir M. Hanash, Haifa Shen, Sankar Mitra, Robert C. Rostomily
Summary: New strategies are urgently needed to improve the dismal median survivals of only ~15-20 months for glioblastoma (GBM) patients after the current standard of care treatment. This study demonstrates the potential importance of PLK1 inhibitors as an adjunct to GBM therapy, with novel mechanisms of action identified for volasertib.
Review
Cell Biology
Satyendra Chandra Tripathi, Disha Vedpathak, Edwin Justin Ostrin
Summary: Cell-mediated immunity is driven by antigenic peptide presentation on major histocompatibility complex (MHC) molecules. Immunoproteasomes, with inducible activity by inflammatory stimuli, play central roles in cancer, autoimmunity, and inflammation by altering the catalytic activity of the proteasome.Understanding the roles of immunoproteasome in different cancers offers insights into developing targeted therapies.
Article
Biochemistry & Molecular Biology
Stefania Colarusso, Federica Ferrigno, Simona Ponzi, Francesca Pavone, Immacolata Conte, Luigi Abate, Elisa Beghetto, Antonino Missineo, Jerome Amaudrut, Alberto Bresciani, Giacomo Paonessa, Licia Tomei, Christian Montalbetti, Elisabetta Bianchi, Carlo Toniatti, Jesus M. M. Ontoria
Summary: By conducting a structure-activity relationship (SAR) study, researchers have discovered compounds with high selectivity and potential anti-Zika virus activity.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Yi-Jia Li, Johannes Francois Fahrmann, Maryam Aftabizadeh, Qianqian Zhao, Satyendra C. Tripathi, Chunyan Zhang, Yuan Yuan, David Ann, Samir Hanash, Hua Yu
Summary: This study demonstrates a critical mechanism underlying chemoresistance in tumors, showing that elevated fatty acid β-oxidation (FAO) activates STAT3, leading to upregulation of ACSL4 and increased phospholipid synthesis, resulting in enhanced mitochondrial integrity and overcoming chemotherapy-induced tumor cell apoptosis.
Article
Oncology
Johannes F. Fahrmann, Ichidai Tanaka, Ehsan Irajizad, Xiangying Mao, Jennifer B. Dennison, Eunice Murage, Julian Casabar, Jeffrey Mayo, Qian Peng, Muge Celiktas, Jody V. Vykoukal, Soyoung Park, Ayumu Taguchi, Oliver Delgado, Satyendra C. Tripathi, Hiroyuki Katayama, Luisa Maren Solis Soto, Jaime Rodriguez-Canales, Carmen Behrens, Ignacio Wistuba, Samir Hanash, Edwin J. Ostrin
Summary: Activation of the NRF2 pathway can lead to upregulation of KYNU in lung cancer, which is associated with tumor immunosuppression.
Correction
Cell Biology
Yi-Jia Li, Johannes Francois Fahrmann, Maryam Aftabizadeh, Qianqian Zhao, Satyendra C. Tripathi, Chunyan Zhang, Yuan Yuan, David Ann, Samir Hanash, Hua Yu
Article
Biology
Giacomo Paonessa, Giulia Siciliano, Rita Graziani, Cristiana Lalli, Ottavia Cecchetti, Cristina Alli, Roberto La Valle, Alessia Petrocchi, Alessio Sferrazza, Monica Bisbocci, Mario Falchi, Carlo Toniatti, Alberto Bresciani, Pietro Alano
Summary: High-throughput screening and validation assays have identified potential antimalarial drugs that can kill both sexual and asexual blood stages of Plasmodium falciparum, thus blocking parasite transmission through mosquitoes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Sung-Eun Lee, Feng Wang, Maison Grefe, Abel Trujillo-Ocampo, Wilfredo Ruiz-Vasquez, Koichi Takahashi, Hussein A. Abbas, Pamella Borges, Dinler Amaral Antunes, Gheath Al-Atrash, Naval Daver, Jeffrey J. Molldrem, Andrew Futreal, Guillermo Garcia-Manero, Jin S. Im
Summary: The purpose of this study is to find immune-related biomarkers to predict effective antitumor immunity in myelodysplastic syndrome (MDS) during immunotherapy and/or hypomethylating agent treatment. Peripheral blood samples from 55 MDS patients were analyzed for immune subsets, T-cell receptor repertoire, gene mutations, and immune-related gene expression profiling. The results showed that clinical responders to immunotherapy exhibited expansion of central memory CD8+ T cells, diverse TCR repertoire, higher mutation burden, and favorable changes in immune landscape.
CLINICAL CANCER RESEARCH
(2023)
Article
Hematology
Uday R. Popat, Oren Pasvolsky, Roland Bassett Jr, Rohtesh S. Mehta, Amanda Olson, Julianne Chen, Amin M. Alousi, Gheath Al-Atrash, Qaiser Bashir, Alison M. Gulbis, Chitra M. Hosing, Jin S. Im, Partow Kebriaei, Issa Khouri, David Marin, Yago Nieto, Betul Oran, Neeraj Saini, Terri Lynn Shigle, Samer A. Srour, Jeremy L. Ramdial, Katayoun Rezvani, Muzaffar H. Qazilbash, Borje S. Andersson, Richard E. Champlin, Elizabeth J. Shpall
Summary: A fractionated myeloablative busulfan regimen was found to be effective in reducing nonrelapse mortality without increasing relapse rate for patients undergoing allogeneic hematopoietic cell transplantation, especially for older patients and those with comorbidities.
Review
Biochemistry & Molecular Biology
Anushree Malviya, Ashok Kumar Ahirwar, Satyendra Chandra Tripathi, Priyanka Asia, Niranjan Gopal, Kirti Kaim
Summary: The global outbreak of COVID-19 has posed a serious health emergency, with many unknown and underestimated risk factors contributing to adverse outcomes, particularly for older individuals or those with comorbidities. Survivors of the Bhopal gas tragedy, due to long-term exposure to harmful substances, are at higher risk of developing adverse outcomes related to COVID-19.
HORMONE MOLECULAR BIOLOGY AND CLINICAL INVESTIGATION
(2021)
Review
Biochemistry & Molecular Biology
Meghana Muthuvattur Pallath, Ashok Kumar Ahirwar, Satyendra Chandra Tripathi, Priyanka Asia, Apurva Sakarde, Niranjan Gopal
Summary: COVID-19 infection primarily affects the respiratory, cardiovascular, and gastrointestinal systems, leading to a cytokine storm; elderly patients or those with chronic underlying diseases are mainly hospitalized, with malnutrition and complications weakening their immune response; appropriate nutritional supplements like Vitamin D and Vitamin C can treat micronutrient deficiencies in COVID-19 patients, enhancing immunity.
HORMONE MOLECULAR BIOLOGY AND CLINICAL INVESTIGATION
(2021)