Article
Biotechnology & Applied Microbiology
Ruida Wang, Jiaqi Zhao, Lei Chen, Jiang Ye, Haizhen Wu, Huizhan Zhang
Summary: In this study, it was identified that LcbR1 functions as a repressor for lincomycin biosynthesis in Streptomyces lincolnensis. LcbR1 suppresses the transcription of lcbR1 itself and SLINC_1596, resulting in an increase in lincomycin production. The expression of lcbR1 may be regulated by GlnR, SLCG_Lrp, and SLCG_2919.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ruida Wang, Yuan Cao, Fanjing Kong, Bingbing Hou, Jiaqi Zhao, Yajing Kang, Jiang Ye, Haizhen Wu, Huizhan Zhang
Summary: The gene ramR(sl) plays a role in the regulation of morphological development and lincomycin biosynthesis in Streptomyces lincolnensis. Deletion of ramR(sl) led to increased lincomycin production and altered expression of known biosynthesis regulators. These findings suggest that RamR may be a potential target for improving antibiotic production in other Streptomyces strains.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Bingbing Hou, Ruida Wang, Jingyun Zou, Feixue Zhang, Haizhen Wu, Jiang Ye, Huizhan Zhang
Summary: The study identified and characterized the redox-sensing regulator Rex(lin) from Streptomyces lincolnensis, showing its effects on cell growth and lincomycin biosynthesis. Results indicated that Rex(lin) can promote transcription of regulatory and structural genes involved in lincomycin production, despite not binding to their promoter regions. Findings contribute to a better understanding of the regulatory mechanism of lincomycin production and form a basis for strain improvements in antibiotic producers.
JOURNAL OF BASIC MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jingyun Zou, Yue Mao, Bingbing Hou, Yajing Kang, Ruida Wang, Haizhen Wu, Jiang Ye, Huizhan Zhang
Summary: This study found that DeoR negatively regulates lincomycin biosynthesis and cellular growth in S. lincolnensis.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Microbiology
Yanping Zhu, Xinyuan Wang, Jing Zhang, Xue Ni, Xia Zhang, Meifeng Tao, Xiuhua Pang
Summary: This study identified targets of OrrA and provided more insights into the role of the orphan response regulator OrrA in Streptomyces through comparing the phenotypes and transcriptomic profiles of wild-type and Delta orrA strains. The interaction of OrrA with the wblA promoter was validated by a pull-down assay, and overexpression of either sco1375 or wblA in Delta orrA partially restored the wild-type phenotype, indicating that these genes mediate some of the effects of OrrA.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei Wu, Yajing Kang, Bingbing Hou, Jiang Ye, Ruida Wang, Haizhen Wu, Huizhan Zhang
Summary: AtrA-lin, a homolog of AtrA, was found to play an important role in lincomycin biosynthesis in Streptomyces lincolnensis. Disruption of atrA-lin led to decreased lincomycin production, while complementation restored production to wild-type levels. The disruption of atrA-lin did not affect cell growth or morphology. Further studies revealed that atrA-lin disruption hindered the transcription of regulatory and structural genes in the lincomycin biosynthesis gene cluster. Restoration of atrA-lin expression partially rescued the transcription of these genes. Importantly, AtrA-lin was found to directly bind to the promoter region of a regulatory gene, lmbU. This study enhances our understanding of the functional diversity of AtrA homologs and the regulation of lincomycin biosynthesis.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xu Yang, Yiqun Wang, Guiyang Liu, Zixin Deng, Shuangjun Lin, Jianting Zheng
Summary: The molecular mechanism of transcription activation by ScZur in Streptomyces coelicolor has been deciphered through cryo-EM structures and biochemical assays. ScZur binds to Zur-box sequences and interacts with Sc RNA polymerase and promoter DNA. These interactions are crucial for the transcription initiation of housekeeping genes in Streptomyces coelicolor.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Yurong Xu, Jing Yi, Yuanzhong Kai, Binglin Li, Meng Liu, Qihua Zhou, Jingru Wang, Ruihua Liu, Hang Wu
Summary: This study used gSELEX to capture SLCG_2919-targeted sequences and revealed its regulatory role in multiple genes, such as SLCG_6675 and SLCG_6579, expanding the understanding of SLCG_2919's control over lincomycin production.
JOURNAL OF BASIC MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Keiichiro Mukai, Tomoko Shibayama, Yu Imai, Takeshi Hosaka
Summary: Lincomycin can stimulate the growth and secondary metabolism of actinomycetes in a concentration-dependent manner. This study investigates the molecular mechanism underlying these concentration-dependent positive effects by exploring the dynamic changes in the ribosome and related factors in the presence of lincomycin.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ruida Wang, Tianyu Zhou, Fanjing Kong, Bingbing Hou, Jiang Ye, Haizhen Wu, Huizhan Zhang
Summary: By analyzing two-component systems (TCSs) in Streptomyces lincolnensis, we identified six novel regulators related to lincomycin biosynthesis. Among them, AflQ1-Q2 was found to have inhibitory effects on lincomycin biosynthesis. The repression mechanism involved AflQ1 binding to a 16-bp motif and inhibiting the transcription and expression of eight operons in the lincomycin synthetic cluster. This study highlights the potential of targeting the highly conserved TCS AflQ1-Q2 for the construction of antibiotic high-yield strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Haiyan Wang, Yuqing Liu, Xu Cheng, Yanyan Zhang, Shanshan Li, Xiangjing Wang, Wensheng Xiang
Summary: This study deciphered the regulatory function of a previously unidentified regulatory protein KelR on several biosynthetic gene clusters (BGCs) including mil in Streptomyces bingchenggensis and demonstrated titer improvement of MIL A3/A4 through manipulation of KelR and other BGCs.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Microbiology
Caiyun Li, Le Li, Luyao Huang, Aiying Li
Summary: The study reveals that ActVI-ORFA is a pleiotropic regulatory factor that controls multiple physiological pathways, including secondary metabolite production, probably via an indirect mode. The regulatory mechanism of ActVI-ORFA remains unclear, but it is known to positively control the production of actinorhodin in Streptomyces coelicolor.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Organic
Zhi Kai Guo, Yong Chao Wang, Ying Zi Tan, Ailiman Abulaizi, Zi Jun Xiong, Shi Qing Zhang, Yang Yang, La Ying Yang, Jing Shi
Summary: Chemical investigation of Streptomyces sp. NA07423 led to the discovery of two unreported macrolactams, nagimycins A(1) and B (2). Their structures were elucidated by NMR, HRESIMS, X-ray crystallography, and comparison of experimental and theoretical ECD spectra. The nagimycins have a unique butenolide moiety rarely found in ansamycin antibiotics. Genome analysis revealed the putative biosynthetic gene cluster for nagimycins, and a likely biosynthetic pathway was proposed. Notably, compounds 1 and 2 exhibited potent antibacterial activity against two pathogenic Xanthomonas bacteria.
Article
Microbiology
Bingbing Tu, Yue Mao, Ruida Wang, Yajing Kang, Jiang Ye, Huizhan Zhang, Haizhen Wu
Summary: This study identified an alternative sigma factor, sigma(L)(sl), in Streptomyces lincolnensis, which plays a direct regulatory role in lincomycin biosynthesis. Deletion of sigL(sl) leads to increased cell growth but decreased lincomycin production, while sigma(L)(sl) boosts lincomycin biosynthesis by directly stimulating the transcription of specific genes.
JOURNAL OF BASIC MICROBIOLOGY
(2023)
Article
Biology
Ning Liu, Hanye Guan, Guoqing Niu, Lingjuan Jiang, Yue Li, Jihui Zhang, Jine Li, Huarong Tan
Summary: The study found that the exogenous activator SsaA can directly activate MRD biosynthesis in Streptomyces, regulating MRD production by binding to the promoter regions of mrd. Further experiments demonstrated the essential role of the 115-150 amino acid fragment in the SSGG-02995 region for activating MRD biosynthesis.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Review
Chemistry, Analytical
Xiaoran Roger Liu, Richard Y-C Huang, Feifei Zhao, Guodong Chen, Li Tao
Summary: This article discusses the application of mass spectrometry (MS) technology in epitope mapping of protein therapeutics. Through reversible and irreversible labeling methods such as hydrogen/deuterium exchange, the changes in solvent accessibilities during binding interactions can be assessed. These methods have been widely used in characterizing the binding interface within immunocomplexes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Medicine, Research & Experimental
Ziyang Dong, Yang Li, Liyuan Tao, Hua Tian
Summary: The use of tourniquet in total knee arthroplasty is currently a topic of debate. Some scholars argue against its application due to potential quadriceps injury and side effects, while others believe it has no significant adverse impact. This prospective trial aims to investigate the effects of tourniquet application on quadriceps morphology and function, providing evidence and guidance for its clinical use.
Article
Oncology
Xin Liu, Liyuan Tao, Mopei Wang, Haiyan Li, Weixian Xu
Summary: A retrospective study of 212 women with HER2-positive breast cancer treated with chemotherapy and trastuzumab found that the ABSDELL model formed by LASSO-Cox regression analysis can effectively predict the probability of LVEF decline in breast cancer patients treated with trastuzumab.
CLINICAL BREAST CANCER
(2023)
Article
Ophthalmology
Qianqian Lan, Yiyun Liu, Fan Xu, Min Li, Yaxin Li, Tingting Yang, Tong Sun, Gang Yao, Baikai Ma, Liyuan Tao, Xin Xiao, Xing Lin Feng, Siming Zeng, Hong Qi
Summary: This study aimed to evaluate the cost-effectiveness of different strategies for bilateral cataract surgery, providing references for healthcare policymakers and surgeons.
OPHTHALMOLOGY AND THERAPY
(2022)
Article
Nutrition & Dietetics
Min Du, Liyuan Tao, Min Liu, Jue Liu
Summary: The mutual relationship between frailty and healthy behavior and its effect on respiratory diseases mortality were investigated in this study. Using a large sample cohort study, the researchers found that frailty was associated with higher risks of total respiratory diseases mortality, influenza and pneumonia mortality, and chronic lower respiratory diseases mortality. Healthy behavior partially mediated this association.
Article
Endocrinology & Metabolism
Xiaoyan Hao, Honghai He, Liyuan Tao, Peng Wang
Summary: This study explored the relationship between serum homocysteine levels, body composition, and the likelihood of having nonalcoholic fatty liver disease (NAFLD) in Chinese healthcare workers. The results showed that NAFLD patients had higher body mass index, waistline, neck circumference, abdominal visceral fat area, total cholesterol, triglycerides, high-density lipoprotein cholesterol, glucose, and homocysteinemia compared to controls. Additionally, skeletal-muscle content was associated with a lower risk of NAFLD, while factors such as BMI, waistline, neck circumference, and hyperhomocysteinemia were associated with a higher risk of NAFLD. These findings may offer a novel approach for diagnosing NAFLD.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Chang Zhang, Cheng Wang, Xin Guo, Huiyu Xu, Zihao Qin, Liyuan Tao
Summary: This study found that green space within a 500 m buffer around schools is associated with a lower personal myopia risk among adolescents and a lower prevalence of myopia in schools. Therefore, it is suggested to prioritize green space within a 500 m buffer around schools in prevention and control activities.
JMIR PUBLIC HEALTH AND SURVEILLANCE
(2023)
Article
Nutrition & Dietetics
Liyuan Tao, Jiaojiao Liao, Rui Zheng, Xiaoyu Zhang, Hongcai Shang
Summary: The aim of this study was to investigate the positive effects of drinking herbal tea and tea on activities of daily living (ADL) in the elderly. The study used data from the Chinese longitudinal healthy longevity survey (CLHLS) and divided the participants into three groups based on their frequency of consuming herbal tea and tea. The results showed that frequent consumption of herbal tea could effectively reduce the incidence of ADL disability, while the effect of tea drinking was relatively weaker.
Article
Medicine, General & Internal
Wenxin Yan, Chenyuan Qin, Liyuan Tao, Xin Guo, Qiao Liu, Min Du, Lin Zhu, Zhongdan Chen, Wannian Liang, Min Liu, Jue Liu
Summary: Inequalities in human resources for health (HRH) have been decreasing globally from 1990 to 2019, but they still persist. All cause mortality and most types of cause specific mortality were relatively higher in countries and territories with a limited health workforce.
BMJ-BRITISH MEDICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Chenyuan Qin, Min Du, Yaping Wang, Qiao Liu, Wenxin Yan, Liyuan Tao, Min Liu, Jue Liu
Summary: To explore the willingness to receive the fourth dose of COVID-19 vaccine and its influencing factors, a national and anonymous survey was conducted in mainland China. The acceptance rate of the fourth dose was found to be 81.1%, while it was 72.6% for a heterologous booster. Confidence in the current domestic situation and uncertainty about extra protection were the main reasons for vaccine hesitancy. Factors such as perceived benefits, cues to action, perceived barriers, self-efficacy, sex, age, vaccination history, social media usage, and satisfaction with the government's response to COVID-19 also influenced vaccination intention.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Public, Environmental & Occupational Health
Zihao Tao, Yuting Feng, Jue Liu, Liyuan Tao
Summary: This study aimed to investigate trends and disparities in sleep quality and duration among older adults in China between 2008 and 2018. The results showed an increased prevalence of poor sleep quality and short sleep duration among older adults during this period. Factors such as gender, economic status, number of chronic diseases, underweight, and poor self-reported quality of life and health were associated with poor sleep quality and short sleep duration. Therefore, more attention should be paid to sleep problems among older adults and early interventions should be implemented to improve sleep quality and ensure adequate sleep time.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Medicine, General & Internal
Yannan Chen, Jiawei Qin, Liyuan Tao, Zhizhen Liu, Jia Huang, Weilin Liu, Ying Xu, Qiang Tang, Yongguo Liu, Zhuhong Chen, Shangjie Chen, Shengxiang Liang, Cong Chen, Jinjin Xie, Jue Liu, Lidian Chen, Jing Tao
Summary: This study compared the effects of tai chi chuan and fitness walking on cognitive function in older adults with T2D and MCI. The results showed that tai chi chuan was more effective than fitness walking in improving cognitive function, suggesting its potential clinical use as an exercise intervention for older adults with T2D and MCI.
Article
Anesthesiology
Wan-Jie Gu, Xiang-Jie Duan, Xiao-Zhu Liu, Yun Cen, Li-Yuan Tao, Jun Lyu, Hai-Yan Yin
Summary: This retrospective cohort study found that the use of magnesium sulfate in critically ill patients with sepsis was associated with lower mortality rates. This association was observed regardless of the baseline serum magnesium status. The use of magnesium sulfate was also associated with lower ICU mortality, in-hospital mortality, and the need for renal replacement therapy.
BRITISH JOURNAL OF ANAESTHESIA
(2023)
Article
Plant Sciences
Liyuan Tao, Jiaojiao Liao, Xiaoyu Zhang, Rui Zheng, Hongcai Shang
Summary: This study analyzed the effect of consuming medicinal plants such as Panax ginseng on all-cause mortality and cognitive impairment. The results showed that frequent and occasional use of medicinal plants significantly reduced the risk of all-cause mortality. Frequent use of medicinal plants also decreased the risk of cognitive impairment.
Article
Urology & Nephrology
Guohua He, Liyuan Tao, Chenglong Li, Xuhui Zhong, Haibo Wang, Jie Ding
Summary: The study aimed to investigate the spectrum of biopsy-proven kidney disease in Chinese children. The most common pathological findings were immunoglobulin A vasculitis with nephritis (29.17%) and IgA nephropathy (22.70%). The pathological spectrum of kidney biopsy changed over time.
JOURNAL OF NEPHROLOGY
(2023)