Article
Agriculture, Dairy & Animal Science
Rita Benitez, Yolanda Nunez, Miriam Ayuso, Beatriz Isabel, Miguel A. Fernandez-Barroso, Eduardo De Mercado, Emilio Gomez-Izquierdo, Juan M. Garcia-Casco, Clemente Lopez-Bote, Cristina ovilo
Summary: The study investigated the effects of developmental stage, breed, and diet energy source on the genome-wide expression, meat quality traits, and tissue composition of biceps femoris muscle in growing pure Iberian and Duroc pigs. Results showed significant transcriptome changes along time in Iberian animals related to growth and tissue development, while breed differences were observed in functions related to muscle development and cytoskeleton organization.
Article
Cell Biology
Mualla Ozcan, Zhen Guo, Carla Valenzuela Ripoll, Ahmed Diab, Antonino Picataggi, David Rawnsley, Aynaz Lotfinaghsh, Carmen Bergom, Jeff Szymanski, Daniel Hwang, Aarti Asnani, Mikhail Kosiborod, Jie Zheng, Robert J. Hayashi, Pamela K. Woodard, Attila Kovacs, Kenneth B. Margulies, Joel Schilling, Babak Razani, Abhinav Diwan, Ali Javaheri
Summary: Fasting strategies are being investigated in patients on chemotherapy. Alternate-day fasting was found to aggravate doxorubicin cardiotoxicity and increase mortality and impaired cardiac function in mice. The study also showed that TFEB and GDF15 pathways contribute to doxorubicin-induced heart failure.
Article
Physiology
Pere Carulla, Miriam Badia-Villanueva, Sergi Civit, Montserrat Carrascal, Joaquin Abian, David Ricart-Jane, Miquel Llobera, Albert Casanovas, M. Dolores Lopez-Tejero
Summary: This study reveals the distribution and regulation of LPL proteoforms in different rat tissues, as well as the reversible regulation during fasting and refeeding. The results indicate that LPL proteoforms are functionally diverse and play a crucial role in the regulation of LPL under physiological conditions.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Chen Sun, Shuyu Mao, Siyu Chen, Wenxiang Zhang, Chang Liu
Summary: PPARs have been identified as crucial regulators of lipid and glucose homeostasis and have the potential to improve metabolic syndromes. Adipose tissue plays a significant role in insulin resistance and metabolic syndromes, with PPARs playing essential roles in adipocyte biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Junli Feng, Xi Chen, Shitong Wang, Jian Zhang, Qingcheng Wang, Shunyuan Guo, Qing Shen
Summary: In this study, the metabolic effects of seafood-derived plasmalogens (Pls) on high fat diet-induced hyperlipidemia in zebrafish were evaluated. It was found that Pls supplementation effectively alleviated obesity symptoms and reduced levels of total hepatic cholesterol and triglycerides. The study also revealed that Pls mainly altered lipid metabolism pathways and the TCA cycle, induced the overexpression of anti-oxidation enzymes, and reduced disease biomarkers and gut microbiota-derived metabolites.
Article
Endocrinology & Metabolism
Aaron R. Cox, Natasha Chernis, Kang Ho Kim, Peter M. Masschelin, Pradip K. Saha, Shawn M. Briley, Robert Sharp, Xin Li, Jessica B. Felix, Zheng Sun, David D. Moore, Stephanie A. Pangas, Sean M. Hartig
Summary: The research shows that the expression of Ube2i in mature adipocytes is crucial for the expansion of white adipose tissue. Deletion of Ube2i in fat cells compromises and diminishes adipocyte function, leading to WAT inflammation and ectopic lipid accumulation in the liver.
MOLECULAR METABOLISM
(2021)
Article
Agriculture, Dairy & Animal Science
Madison N. Myers, Ursula Abou-Rjeileh, Miguel Chirivi, Jair Parales-Giron, Adam L. Lock, Joseph Tam, Maya Zachut, Andres Contreras
Summary: Amplified adipose tissue (AT) lipolysis and suppressed lipogenesis characterize the periparturient period of dairy cows. Activation of cannabinoid-1 receptor (CB1R) can reduce lipolysis and enhance lipogenesis in nonlactating non-gestating (NLNG) cows, but it has no direct effect on lipolysis in periparturient cows. CB1R activation can enhance adipogenesis and lipogenesis in the AT of NLNG cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Michelle Berenice Vega Joubert, Paola Ingaramo, Maria Eugenia Oliva, Maria Eugenia D'Alessandro
Summary: The study revealed that chronic consumption of a sucrose-rich diet can lead to dyslipidemia, hyperglycemia, hepatic lipid accumulation, and liver injury in rats. However, incorporating chia seed into the diet for 3 months showed beneficial effects in reversing these issues and improving liver health by reducing oxidative stress.
Article
Physiology
Atefeh Berenjian, Mohammad Reza Bakhtiarizadeh, Abdollah Mohammadi-Sangcheshmeh, Seyed Davood Sharifi
Summary: Supplementing with ?-3 fatty acids can reduce the harmful effects of stress. This study used RNA-sequencing technology to analyze the liver transcriptome of laying hens fed a diet supplemented with ?-3, revealing different gene expression profiles and potential pathways involved in response to stress. The results showed that the differentially expressed genes were enriched in processes such as steroid and cholesterol biosynthesis, fatty acid metabolism, AMPK signaling pathway, fatty acid biosynthesis, and immune response. Downregulation of UNC13B and ADRA1B genes in the stress_?-3 group may play a role in facilitating the response to stress. This study enhances our understanding of the molecular responses to stress in laying hens fed a diet supplemented with ?-3 fatty acids.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Lingyu Zhang, Fengna Li, Qiuping Guo, Yehui Duan, Wenlong Wang, Yuhuan Yang, Yunju Yin, Saiming Gong, Mengmeng Han, Yulong Yin
Summary: The supplementation of BCAAs (leucine/isoleucine/valine = 2:1:1 or 2:1:2) in protein-restricted diets can improve growth performance and carcass characteristics in finishing pigs. It also modulates lipid metabolism by accelerating the secretion of adipokines and fatty acid oxidation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jilong Han, Tingting Guo, Yaojing Yue, Zengkui Lu, Jianbin Liu, Chao Yuan, Chune Niu, Min Yang, Bohui Yang
Summary: Tail adipose tissue plays a crucial role in enhancing environmental tolerance for sheep, and this study aimed to explore the development mechanisms of this trait. Proteomic analysis revealed differentially expressed proteins in the tail adipose tissues between fat-tailed and thin-tailed sheep, mainly related to metabolism pathways and the peroxisome proliferator activated receptor signaling pathway. Up-regulated proteins like ACS L1, HSD17 beta 4, FABP4 may contribute to tail fat deposition by promoting adipocyte proliferation and fat accumulation.
Review
Food Science & Technology
Lei Wang, Rong Wang, Xiaoyan Yu, Yuhuan Shi, Shengnan Li, Yongfang Yuan
Summary: This review summarizes the effects of energy restriction on macrophages in various diseases, offering valuable guidance for future research and insights into the clinical applications of calorie restriction and fasting.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Hao Gu, Mailin Gan, Linghui Wang, Yiting Yang, Jinyong Wang, Lei Chen, Shunhua Zhang, Ye Zhao, Lili Niu, Dongmei Jiang, Yiwu Chen, Anan Jiang, Linyuan Shen, Li Zhu
Summary: This study investigates the expression differences of tsRNA in subcutaneous adipose tissue of obese and lean pigs, and reveals that tsRNA may participate in the regulation of energy metabolism in porcine adipocytes by influencing MAPK, AMPK, and other signaling pathways, thus promoting lipid deposition and contributing to obesity development.
Article
Cell Biology
Nadav Kislev, Shira Eidelheit, Shaked Perlmutter, Dafna Benayahu
Summary: In this study, a novel machine learning-based method was developed for analyzing lipid droplets (LDs) by segmenting phase-contrast images. This method enables accurate and label-free quantification of LD-related parameters and analysis of LD dynamics. It can be applied in different metabolic conditions to study LD biogenesis and function.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Genetics & Heredity
Isabel Reinisch, Ingeborg Klymiuk, Helene Michenthaler, Elisabeth Moyschewitz, Markus Galhuber, Jelena Krstic, Magnus Domingo, Fangrong Zhang, Michael Karbiener, Nemanja Vujic, Dagmar Kratky, Renate Schreiber, Michael Schupp, Georgia Lenihan-Geels, Tim J. Schulz, Roland Malli, Tobias Madl, Andreas Prokesch
Summary: This study reveals a fasting-induced pathway involving p53 that activates the transcription of miR-92a-1-5p, which in turn decreases Slc2a5 expression in thermogenic adipocytes. It suggests fructose as an energy substrate in thermogenic adipocytes.
FRONTIERS IN GENETICS
(2022)
Article
Agriculture, Dairy & Animal Science
Federico Melenchon, Eduardo de Mercado, Hector J. Pula, Gabriel Cardenete, Fernando G. Barroso, Dmitri Fabrikov, Helena M. Lourenco, Maria-Fernanda Pessoa, Leidy Lagos, Pabodha Weththasinghe, Marcos Cortes, Cristina Tomas-Almenar
Summary: Reducing dependence on fishmeal is a major challenge for sustainable aquaculture, and insects are one of the most promising alternatives. This study found that yellow mealworm is the best alternative for rainbow trout, possibly due to changes in protein utilization and intestinal histology. Insect meals also showed potential as functional ingredients in preventing tissue damage.
Article
Biology
Juan M. Vazquez-Mosquera, Aitor Fernandez-Novo, Martin Bonet-Bo, Natividad Perez-Villalobos, Jose L. Pesantez-Pacheco, Maria Luz Perez-Solana, Eduardo de Mercado, Juan Carlos Gardon, Arantxa Villagra, Francisco Sebastian, Sonia Salome Perez-Garnelo, Daniel Martinez, Susana Astiz
Summary: This study presents the results of embryo transfer procedures in Japanese Black cattle under Spanish management and environmental conditions. The study demonstrates that adequate embryo productivity can be achieved in this breed, and the recipients' metabolites play a crucial role in achieving pregnancy. Heat stress negatively impacts conception rates, while larger parity and ET number correspond to higher conception rates.
Article
Multidisciplinary Sciences
Ana Heras-Molina, Yolanda Nunez, Rita Benitez, Jose Luis Pesantez-Pacheco, Consolacion Garcia-Contreras, Marta Vazquez-Gomez, Susana Astiz, Beatriz Isabel, Antonio Gonzalez-Bulnes, Cristina Ovilo
Summary: The hypothalamic transcriptome of pure Iberian and crossbred piglets at 60 days-old was evaluated in this study. The results showed that gender and genotype have an impact on the hypothalamic transcriptome. The gene expression differences between genotypes were more significant in males, with a higher expression of genes related to mitochondrial activity in pure Iberian males and higher expression of SIRT3 regulator in crossbred males. Such differences were not found in females, except for some genes related to the tricarboxylic acid cycle.
Article
Agriculture, Dairy & Animal Science
Juan M. Vazquez-Mosquera, Eduardo de Mercado, Aitor Fernandez-Novo, Juan C. Gardon, Jose L. Pesantez-Pacheco, Maria Luz Perez-Solana, Angel Revilla-Ruiz, Daniel Martinez, Arantxa Villagra, Francisco Sebastian, Sonia S. Perez-Garnelo, Susana Astiz
Summary: Cattle growth is crucial for beef production. This study shows that purebred and crossbred Wagyu steers raised in Spain with high welfare standards and a local diet high in olein content exhibited appropriate growth and fattening rates. Wagyu and Wangus steers had similar growth rates, but purebred Wagyu may be more profitable.
Article
Multidisciplinary Sciences
C. Ovilo, N. Trakooljul, Y. Nunez, F. Hadlich, E. Murani, M. Ayuso, C. Garcia-Contreras, M. Vazquez-Gomez, A. I. Rey, F. Garcia, J. M. Garcia-Casco, C. Lopez-Bote, B. Isabel, A. Gonzalez-Bulnes, K. Wimmers, M. Munoz
Summary: This study evaluated a panel of DNA markers associated with traits related to muscle growth, fatness, meat quality and metabolism, and performed an association study in a commercial pig population. The results confirmed known SNP effects and revealed interesting effects of new SNPs in less known genes. These findings provide a valuable basis for future marker-assisted selection strategies in swine.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Ana Heras-Molina, Rosa Escudero, Jose L. Pesantez-Pacheco, Consolacion Garcia-Contreras, Marta Vazquez-Gomez, Susana Astiz, Cristina Ovilo, Antonio Gonzalez-Bulnes, Beatriz Isabel
Summary: The study investigated the effects of maternal dietary supplementation with hydroxytyrosol and n3 polyunsaturated fatty acids (n3-PUFA) on the fatty acid composition of offspring tissues in Iberian pigs. The results showed higher levels of n3-PUFA in the supplemented fetuses' plasma and tissues, which may have implications for piglets' health and human dietary recommendations.
Article
Agriculture, Dairy & Animal Science
Cristina Tomas-Almenar, Eduardo de Mercado
Summary: Limited attention has been given to sperm thawing protocols, but it is important for endangered species or breeds. This study aimed to determine the most optimal thawing protocol for Iberian boar sperm. The findings suggest that thawing at 70 degrees C for 8 s with the inclusion of cyclodextrins loaded with cholesterol (CLC) in the extender is the best protocol.
Article
Agriculture, Dairy & Animal Science
Juan M. Vazquez-Mosquera, Aitor Fernandez-Novo, Eduardo de Mercado, Marta Vazquez-Gomez, Juan C. Gardon, Jose L. Pesantez-Pacheco, Angel Revilla-Ruiz, Raquel Patron-Collantes, Maria L. Perez-Solana, Arantxa Villagra, Daniel Martinez, Francisco Sebastian, Sonia S. Perez-Garnelo, Susana Astiz
Summary: This study compared the fat profile, health-related indexes, and metabolic biomarkers of Wagyu, Wagyu-by-Angus (Wangus), and Angus-by-Charolaise-Limousine (ACL) beef produced in a Spanish fattening system with high-olein diets. The results showed that blood lipid-related metabolites were higher in Wagyu and Wangus beef compared to ACL beef, while glucose was lower. Wagyu and Wangus beef had higher fat infiltration and healthier fat profiles compared to ACL beef.
Article
Biochemistry & Molecular Biology
Hernan D. Laviano, Gerardo Gomez, Maria Munoz, Juan M. Garcia-Casco, Yolanda Nunez, Rosa Escudero, Ana Heras Molina, Antonio Gonzalez-Bulnes, Cristina Ovilo, Clemente Lopez-Bote, Ana I. Rey
Summary: Modifying the composition of a sow's milk, specifically by supplementing with vitamin E and/or hydroxytyrosol, can improve the intestinal health and growth of piglets. The study found that vitamin E supplementation increased the levels of C18:1n-7 in colostrum, while hydroxytyrosol increased the levels of polyunsaturated fatty acids. Vitamin E supplementation also decreased certain fatty acids and increased ?-6-desaturase activity in 7-day milk. The lowest levels of milk lipid oxidation were observed in the vitamin E supplemented group, while hydroxytyrosol supplementation increased oxidation.
Article
Biochemistry & Molecular Biology
Hernan D. Laviano, Gerardo Gomez, Rosa Escudero, Yolanda Nunez, Juan M. Garcia-Casco, Maria Munoz, Ana Heras-Molina, Clemente Lopez-Bote, Antonio Gonzalez-Bulnes, Cristina Ovilo, Ana I. Rey
Summary: This study evaluates the effects of dietary supplementation of vitamin E, hydroxytyrosol, or the combination of the two on gut health and piglet growth. The results suggest that vitamin E supplementation is superior to hydroxytyrosol supplementation in improving intestinal homeostasis and piglet growth.
Article
Biochemistry & Molecular Biology
Gerardo Gomez, Hernan D. Laviano, Juan M. Garcia-Casco, Rosa Escudero, Maria Munoz, Ana Heras-Molina, Antonio Gonzalez-Bulnes, Cristina Ovilo, Clemente Lopez-Bote, Ana I. Rey
Summary: Different feeding strategies are being applied to sows in order to obtain homogeneous piglets' weights and improved health status. This study evaluated the effects of dietary supplementation of vitamin E (VE), hydroxytyrosol (HXT), or their combination on the growth pattern and oxidative status of piglets, as well as the factors contributing to these effects. The results showed that both VE and HXT improved the oxidative status of sows and piglets, and modified the growth pattern of the piglets depending on the growing period. The study also found that colostrum composition played a significant role in the growth patterns of the piglets.
Article
Agriculture, Dairy & Animal Science
Sylwia Dudkiewicz, Patricia Peris-Frau, Helena Nieto-Cristobal, Julian Santiago-Moreno, Eduardo de Mercado, Manuel Alvarez-Rodriguez
Summary: This study investigated the capacitation process of boar sperm and found that the combination of bicarbonate and BSA contributed to increasing the proportion of capacitated sperm and mitochondrial membrane potential. However, caffeine did not significantly influence sperm capacitation in boars.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Armando Caballero, Almudena Fernandez, Beatriz Villanueva, Miguel A. Toro
Summary: This study investigates the accuracy of estimators of inbreeding based on molecular markers in different mating systems and artificially selected populations. The results show that when the allele frequencies of the base population are known, all estimators based on marker allele frequencies provide accurate estimates of inbreeding coefficients and inbreeding depression. When the allele frequencies are unknown, F-LH1 is the most correlated estimator with the actual values, and F-YA2 provides the most accurate estimates of inbreeding depression.
GENETICS SELECTION EVOLUTION
(2022)
Article
Genetics & Heredity
L. Gomez-Raya, E. Gomez Izquierdo, E. de Mercado de la Pena, F. Garcia-Ruiz, W. M. Rauw
Summary: This study applies the concept of genetic exclusion to estimate the rate of rejection of parent-offspring relationships and genotyping errors in animal experiments and genomic selection. The results show that it is possible to establish or verify first-degree relationships and estimate genotyping errors using this method.