Article
Medicine, Research & Experimental
Xiaowei Xing, Shuang Guo, Yusheng Liu, Jiangying Kuang, Zhiwei Huang, Xin Wang, Qinghua Lu
Summary: SAX shows potential therapeutic effects in diabetic nephropathy by reducing renal cell apoptosis and pathological outcomes, likely through downregulating the expression of caspase 3 and PARP-1 in the apoptosis pathway.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sophiko Tsikarishvili, Margarita Karapetian, Nina Kulikova, Giorgi Zaalishvili
Summary: This study evaluated the role of poly(ADP)ribosylation on rubitecan induced caspase dependent cell death in zebrafish embryo. The data suggests that Top1 poisoning by rubitecan induces caspase mediated apoptosis, which can be reduced by PARP inhibitor olaparib. Overall, zebrafish emerges as a valuable model for PARP related biomedical research.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Environmental Sciences
Shanshan Hou, Xiayu Zhang, Xiaofan Ning, Hao Wu, Xinyue Li, Kai Ma, Huifang Hao, Chunping Lv, Chunrui Li, Zhongjun Du, Haiying Du, Minghua Jin
Summary: This study explores the role of ROS-mediated PARP/AIF apoptosis signaling pathway in MeHg-induced cell death. The results suggest that increased levels of ROS and calcium ion, as well as the activation of caspase cascades and PARP, are involved in MeHg-induced apoptosis. The inhibition of ROS production can reduce the apoptosis rate. The PARP/AIF apoptotic pathway is independent of the caspase pathway and regulates it.
ENVIRONMENTAL TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Natalya V. Maluchenko, Alexey V. Feofanov, Vasily M. Studitsky
Summary: PARP-1 plays crucial roles in cell cycle regulation, DNA repair, transcription, and replication, with its hyperactivity promoting chronic pathological processes and moderate activation supporting tumor cell survival. Therefore, PARP inhibitors are considered promising agents for the treatment of tumors and metabolic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nathan P. P. Mirtallo Ezzone, Gerardo D. D. Anaya-Eugenio, Ermias Mekuria Addo, Yulin Ren, A. Douglas Kinghorn, Esperanza J. J. Carcache de Blanco
Summary: The present study investigated the potential anticancer properties of corchorusoside C (1), a cardenolide from Streptocaulon juventas, using a zebrafish model and DU-145 prostate cancer cells. The results showed that compound 1 selectively inhibited the expression of NF-kappa B pathway elements in TNF-alpha induced zebrafish and cell lines. Molecular docking studies revealed the binding interactions of 1 with various proteins involved in cancer pathways. Furthermore, the toxicity profile of 1 in zebrafish was found to be favorable compared to other anticancer drugs. Overall, these findings suggest that compound 1 may be a promising treatment option for prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Junkang Ren, Xu Quan, Ying Liu, Jiani Li, Xiaoyu Zhang, Zhiyu Li, Xiaomeng Zhang
Summary: Inhibition of PARP has been successful in clinical treatment of homologous recombination-deficient malignancy. Recent study shows that both PARP-1 inhibition and DNA trapping contribute to the efficacy in BRCA mutant tumors, and toxicities result from poor selectivity of PARP-1 over PARP-2. A series of compounds were synthesized and compound 8m was identified as a highly potent and selective inhibitor and DNA trapper of PARP-1.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Organic
Mandapati Bhargava Reddy, Kesavan Prasanth, Ramasamy Anandhan
Summary: A metal-free photoredox catalyzed process using proton-coupled electron transfer was developed for the controlled radical cyclization cascade of o-alkynylated benzamides. This reaction allows the addition of amidyl N-centered radical to C-C triple bond under mild conditions and the synthesis of 3-hydroxyisoindolin-1-ones and phthalimides. Additionally, a time tunable synthesis of 3-hydroxyisoindolin-1-ones and phthalimides via beta-carbonyl-C(sp(3)) bond cleavage was achieved under visible light irradiation.
Article
Oncology
Saba Jahangiri, Samideh Khoei, Sepideh Khoee, Majid Safa, Sakine Shirvalilou, Vahid Pirhajati Mahabadi
Summary: The study evaluated the efficacy of chemo-magnetic hyperthermia in inducing apoptosis in human cancer cell lines. Results showed that the combined treatment significantly increased apoptosis compared to chemotherapy or hyperthermia alone. Up-regulation of apoptotic effector proteins and inhibition of cell viability were observed, indicating the synergistic effect of hyperthermia and chemotherapy in damaging cancer cells.
CANCER NANOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huibin Xu, Xiaoman Shen, Xiangke Li, Xiaohui Yang, Chuan Chen, Duqiang Luo
Summary: The natural product DSE2, derived from fungi, has potent anticancer activity but its mechanism is unclear. It effectively inhibits gastric cancer cell growth and induces apoptosis by activating PARP-1 and caspase-3. Inhibition or knockdown of PARP-1 or caspase-3 suppresses DSE2-induced apoptosis.
Article
Biochemistry & Molecular Biology
Shuai Li, Xin-yang Li, Ting-jian Zhang, Ju Zhu, Kai -li Liu, De-pu Wang, Fan-hao Meng
Summary: The novel amide PARP-1 inhibitor R8e exhibited strong anti-tumor activity specifically towards A549 cells. It effectively arrests the cell cycle in the S phase and induces cell apoptosis, showing potential for further development as a lead compound for novel PARP-1 inhibitors.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Applied
Oleh Lukianov, Viktor Tkachuk, Svitlana Shishkina, Leonid Lachmann, Olga Vadzyuk, Petro Borysko, Dmytro Kovalskyy, Isabelle Gillaizeau, Volodymyr Sukach
Summary: In this study, a visible-light-mediated hydroaminoalkylation of pyrimidin-2(1H)-ones via the aza-Giese-type reaction using acridinium dye as a photocatalyst under mild aerobic conditions was reported. Various Biginelli-type 3,4-dihydropyrimidin-2(1H)-ones were prepared in 31-73% yields by using N-Boc protected aminoalkyl trifluoroborates as radical precursors and different pyrimidine-2(1H)-one substrates. The obtained products were further transformed into 3,6,7,7a-tetrahydro-1H-pyrrolo[3,4d]pyrimidine-2,5-dione derivatives, which demonstrated potential as inhibitors of poly (ADP-ribose) polymerase (PARP) enzymes.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Cell Biology
Giovanna De Matteis, Francesco Grandoni, Michele Zampieri, Anna Reale, Maria Carmela Scata
Summary: The study investigated PARP-1 activation in leukocyte subpopulations from bovine milk samples during udder infection, showing a significant increase in PAR content in milk samples from cows with intramammary infection. These results suggest mastitis as a new model for the study of the role of PARP in zoonotic inflammatory diseases, opening a new perspective to the One Health approach.
Article
Biochemistry & Molecular Biology
Shivani Singla, Vinod Kumar, Gopabandhu Jena
Summary: Diabetes and ulcerative colitis are chronic diseases associated with inflammation, dysbiosis, impaired immune function, and infection risk. This study aimed to investigate the role of 3-aminobenzamide (3-AB) in ulcerative colitis-associated diabetes mellitus. The results showed that 3-AB significantly reduced the severity of colitis-associated diabetes mellitus by altering the expression of multiple molecular targets.
Article
Food Science & Technology
David S. Dang, Chandler D. Stafford, Mackenzie J. Taylor, Jared F. Buhler, Kara J. Thornton, Sulaiman K. Matarneh
Summary: The study found that ultrasonication improved beef tenderness by increasing the activities of calpain-1 and caspase-3. These effects may be achieved by increasing cytosolic calcium and inducing mitochondrial dysfunction.
Article
Food Science & Technology
Miwa Takatsuka, Satoru Goto, Kenshiro Kobayashi, Yuta Otsuka, Yohsuke Shimada
Summary: This study investigated the radical scavenging reaction of antioxidants in DPPH and ABTS assays using ESR and developed an analytical method. The results demonstrated that the first component in both assays contributed significantly to the radical scavenging reaction.
Article
Dermatology
Simona Pisanti, Teresa Mencherini, Tiziana Esposito, Valeria D'Amato, Tania Re, Maurizio Bifulco, Rita P. Aquino
Summary: This study critically compares medieval and modern dermocosmetics through the analysis of Trotula de Ruggiero's works. The results highlight Trotula's focus on female care, beauty and well-being, as well as the extraordinary combination of tradition and modernity in her work.
JOURNAL OF COSMETIC DERMATOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Giovanni Falcone, Sonja Kuth, Aldo R. Boccaccini, Rita P. Aquino, Tiziana Esposito, Paola Russo
Summary: To fully utilize the potential of 3D printing, particularly in the case of semisolid extrusion (SSE), the technological challenges need to be addressed. This study focuses on investigating the physicochemical properties of natural polymers, specifically alginate hydrogel, for 3D printing tablets that are easy to swallow. By adding sorbitol as a plasticizer, the aim is to improve the swallowability of the tablets. Different concentrations of sorbitol solution are evaluated to determine the best composition in terms of printing and technological properties. The results highlight a predictable relationship between the amount of sorbitol loaded and the residual mass of the final platforms.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Instruments & Instrumentation
Chiara Amante, Valentina Andretto, Annalisa Rosso, Geraldine Augusti, Stefania Marzocco, Giovanna Lollo, Pasquale Del Gaudio
Summary: This study developed an innovative wound dressing using natural polymers and nanoemulsions. Alginate-pectin in situ gelling powders were spray-dried as carriers for curcumin-loaded nanoemulsions. The nanocomposites formed a gel in a short time upon contact with simulated wound fluid, allowing for good wound transpiration and prolonged release of curcumin.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Michela Pecoraro, Stefania Marzocco, Raffaella Belvedere, Antonello Petrella, Silvia Franceschelli, Ada Popolo
Summary: This study aimed to evaluate the protective effects of Simvastatin on Doxorubicin-induced cardiotoxicity. The results showed that Simvastatin co-treatment significantly reduced oxidative stress, calcium homeostasis disturbance, and apoptosis. Furthermore, Simvastatin co-treatment also affected the expression and cellular localization of Connexin 43, which is involved in cardioprotection. Based on these findings, Simvastatin could be a promising adjuvant in Doxorubicin anticancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michela Pecoraro, Adele Serra, Maria Pascale, Silvia Franceschelli
Summary: Correct protein folding is essential for cellular wellbeing, but accumulation of misfolded proteins in the endoplasmic reticulum (ER) can disrupt cellular homeostasis and cause ER stress. Protein misfolding has been implicated in various human diseases, including cancer, diabetes, and cystic fibrosis. The unfolded protein response (UPR) is activated in response to ER stress and involves the three ER-resident proteins, IRE1 alpha, PERK, and ATF6. This article highlights the importance of correct protein folding and the potential of the drug lumacaftor (Vx-809) in reducing ER stress and inflammation associated with protein misfolding. Rating: 8/10
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Carmela Tommasino, Giulia Auriemma, Carla Sardo, Carmen Alvarez-Lorenzo, Emilia Garofalo, Silvana Morello, Giovanni Falcone, Rita P. Aquino
Summary: In this study, a PCL/INU-PLA hybrid biomaterial was prepared, which showed improved surface properties, swelling ability, and in vitro biodegradation rate compared to PCL alone. The 3D printed scaffolds exhibited dimensional and structural parameters close to the digital model and showed favorable results in biocompatibility and osteogenic activity tests.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Applied
Redouane Lemoui, Patrizia Picerno, Samira Benyahia, Teresa Mencherini, Antonio Vassallo, Maria Francesca Armentano, Labib Noman, Khaled Bouchama, Luca Rastrelli, Rita Patrizia Aquino
Summary: In this study, seven secondary metabolites were isolated and identified from the methanol extract of Stachys brachyclada de Noe ex Coss. leaves. The extract showed inhibition of alpha-glucosidase enzyme, radical scavenging activity, and reduction of oxidative stress level in Human Dermal Fibroblast. Stachysetin was identified as the major compound in the extract.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biology
Chiara Amante, Chiara De Soricellis, Gianni Luccheo, Luigi Luccheo, Paola Russo, Rita Patrizia Aquino, Pasquale Del Gaudio
Summary: The National Institute of Health has found that 80% of chronic infections are linked to biofilms, which contribute to bacterial resistance to antimicrobial agents. A novel mixture composed of N-acetylcysteine (NAC) and various natural ingredients has been developed to enhance NAC's activity against different bacteria and reduce biofilm formation. This mixture has shown increased NAC permeation, fibrinolytic activity, antibiofilm activity, and reduced bacterial adhesion.
Article
Polymer Science
Maria Rosaria Sellitto, Chiara Amante, Rita Patrizia Aquino, Paola Russo, Rosalia Rodriguez-Dorado, Monica Neagu, Carlos A. Garcia-Gonzalez, Renata Adami, Pasquale Del Gaudio
Summary: The use of polysaccharide-based aerogels as drug carriers for wound healing is being explored. Drug-loaded aerogel capsules were produced through prilling in tandem with supercritical extraction. The capsules had a hollow cavity and a thin alginate aerogel layer with good textural properties. These capsules effectively absorbed wound fluid and prolonged drug release due to a formed hydrogel barrier.
Article
Plant Sciences
Tiziana Esposito, Simona Pisanti, Rosanna Martinelli, Rita Celano, Teresa Mencherini, Tania Re, Rita P. Aquino
Summary: This study compared the metabolite profile of Couroupita guianensis bark decoction produced by Amazonian shamans with that obtained under laboratory conditions and investigated their biological properties in wound healing and inflammation. The analysis revealed the presence of polyphenolic compounds and uncommon sulphated derivatives of ellagic acid in the decoction. A new sulphated molecule was identified as the potential active compound responsible for wound healing. The decoction also showed anti-inflammatory effects and potential therapeutic applications in skin diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Patrizia Picerno, Lucia Crasci, Patrizia Iannece, Tiziana Esposito, Silvia Franceschelli, Michela Pecoraro, Virgilio Giannone, Anna Maria Panico, Rita Patrizia Aquino, Maria Rosaria Lauro, Francisca Rodrigues, Ana Margarida Silva
Summary: This study evaluated the biological and technological activity of a dry aqueous extract from Sicilian almond peels, finding significant antioxidant and metalloproteinase inhibition effects. The use of Eudraguard (R) Natural improved the solubility and release of the extract and protected it from degradation and oxidation, prolonging the shelf life of the final product.