Article
Chemistry, Multidisciplinary
Ruoxian Deng, Changwei Li, Xiao Wang, Leilei Chang, Shuangfei Ni, Weixin Zhang, Peng Xue, Dayu Pan, Mei Wan, Lianfu Deng, Xu Cao
Summary: The research indicates that myeloid-lineage cells differentiate into subgroups and regulate periosteal bone formation in response to mechanical loading. Macrophages, a subtype of myeloid-lineage cells, play a crucial role in this process by activating TGF-beta 1 to recruit osteoprogenitors.
Review
Endocrinology & Metabolism
Mackenzie L. Hagan, Vanshika Balayan, Meghan E. McGee-Lawrence
Summary: Mammalian cells use various mechanisms to detect and respond to mechanical loading, including the formation of plasma membrane disruptions (PMD) for tissue adaptation. Repair of PMD is crucial for cell survival and can impact downstream cellular signaling, affecting a wide range of organ systems. The review focuses on PMD formation and repair processes across mechanosensitive tissues, highlighting implications for skeletal mechanobiology and potential therapeutic applications.
Article
Multidisciplinary Sciences
Marcela P. Bernardo, Bruna C. R. da Silva, Ahmed E. Hamouda, Marcelo A. S. de Toledo, Carmen Schalla, Stephan Rutten, Roman Goetzke, Luiz H. C. Mattoso, Martin Zenke, Antonio Sechi
Summary: Bone defects are a major challenge in reconstructive surgery, and 3D-printed scaffolds using fused deposition modelling (FDM) can provide a tailored solution. This study developed 3D-printed porous scaffolds made of polylactic acid/hydroxyapatite (PLA/HA) composites with high ceramic contents, which exhibited mechanical properties similar to trabecular bone and showed no immune-stimulatory properties. Additionally, these PLA/HA scaffolds were shown to promote osteogenic differentiation of human mesenchymal stem cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Jun Li, Ting Zhang, Chenglong Huang, Min Xu, Wenhua Xie, Qilin Pei, Xinxin Xie, Bin Wang, Xi Li
Summary: The study revealed the role of chemerin in bone metabolism, demonstrating its ability to promote osteogenic differentiation and bone formation. This provides a new theoretical basis for chemerin as a therapeutic strategy for osteoporosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hyung-Mun Yun, Mi Hyeon Cho, Hoibin Jeong, Soo Hyun Kim, Yun Hee Jeong, Kyung-Ran Park
Summary: This study investigated the effects of a flavonoid called trifolirhizin (trifol) derived from Sophora flavescens on osteogenic processes during osteoblast differentiation. It was found that trifol promoted osteoblast differentiation markers and stimulated major osteogenic signaling proteins. Trifol also increased osteoblast-mediated bone-forming phenotypes. These findings suggest that trifol may be an effective phytomedicine for bone diseases such as osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yani He, Xiaoyue Guan, Yang Du, Guanzhi Liu, Yingxue Li, Zhichen Wei, Chen Shi, Jianmin Yang, Tiezhou Hou
Summary: The study found that mechanical stress promoted the proliferation of hDPSCs, with key gene expressions increasing on day 7 but decreasing on day 14. A total of eight miRNAs were differentially expressed in response to mechanical stress, affecting various signaling pathways in the development of hDPSCs.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Shaowei Zheng, Haobo Zhong, Hao Cheng, Xu Li, Guowei Zeng, Tianyu Chen, Yucong Zou, Weile Liu, Chunhan Sun
Summary: In this study, a TEOS-hydrogel was designed with self-healing property, low swelling rate, degradability, and good biocompatibility, and capable of inducing osteogenic differentiation of bone marrow mesenchymal stem cells. In rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, demonstrating its potential for treating critical-size segmental bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Martin Montecino, Margarita E. Carrasco, Gino Nardocci
Summary: Genomic DNA in the eukaryotic nucleus is organized into chromatin through stable interactions with histone proteins and other nuclear components, contributing to differential control of access to key genetic information. Epigenetic mechanisms, such as DNA methylation and chromatin remodeling, play critical roles in regulating gene expression by modifying chromatin compaction levels for transcription activation and repression. The review discusses evidence supporting the role of these mechanisms in the transcriptional control of osteoblast-related genes, particularly at the Runx2 and Sp7 genes, which encode master regulators of osteogenic lineage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Mi Eun Kim, Jong Keun Seon, Ju Yeon Kang, Taek Rim Yoon, Jun Sik Lee, Hyung Keun Kim
Summary: BFP-4 induces stronger osteogenic differentiation of BMSCs compared to BMP-7, as well as enhancing ALP activity, calcium concentration, and osteogenic factor production. This suggests that BFP-4-stimulated cell therapy may be a more efficient and cost-effective approach to bone regeneration and formation than BMP-7-based therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Marcel Rodrigues Ferreira, Willian Fernando Zambuzzi
Summary: By analyzing the role of miRNAs in osteogenic differentiation, this study identified potential therapeutic miRNAs and targets in bone healing and tissue engineering through examining miRNAseq expression profiles and constructing an ePPI network.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Anatomy & Morphology
Celio Junior da Costa Fernandes, Ana Flavia Balestrero Cassiano, Flavio Henrique-Silva, Joni Augusto Cirelli, Eduardo Pereira de Souza, Hernan Coaguila-Llerena, Willian Fernando Zambuzzi, Gisele Faria
Summary: Cysteine protease inhibitors, specifically sugarcane-derived cystatin (CaneCPI-5), were shown to negatively modulate adhesion and cytoskeleton remodeling, while also promoting cell migration and matrix metalloproteinase activation in osteoblasts. This study suggests that CaneCPI-5 can trigger mechanisms related to osteoblast differentiation, providing new perspectives for biotechnological approaches to bone disorders.
Article
Cell Biology
Tianyi Wu, Fuli Yin, Nan Wang, Xin Ma, Chaolai Jiang, Lihui Zhou, Yang Zong, Haojie Shan, Wenyang Xia, Yiwei Lin, Zubin Zhou, Xiaowei Yu
Summary: This study found a significant increase in osteogenic markers in BMSCs strained at 8%. The expression of osteogenic markers and mechanosensitive ion channels were significantly enhanced in BMSCs cultured under 8% strain. However, the loss of mechanosensitive ion channel function resulted in the inhibition of osteogenic marker expression.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Xiao Han, Shijia Tang, Lin Wang, Xueqin Xu, Ruhan Yan, Sen Yan, Zhaobin Guo, Ke Hu, Tingting Yu, Mengping Li, Yuqin Li, Feimin Zhang, Ning Gu
Summary: DPSCs cultured in three-dimensional spheroids exhibited enhanced odontogenic differentiation ability, with a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation. Furthermore, DPSCs spheroids showed a stronger osteogenic differentiation tendency than conventionally cultured two-dimensional DPSCs under the stimulation of magnetic iron oxide nanoparticles (IONPs). This suggests that a composite viscoelastic material-based 3D spheroid culture combined with mechanical/magnetic stimulation may provide new insights for dentin or bone regeneration technology.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Cell Biology
Xiaoying Chen, Wei Xie, Ming Zhang, Yuhan Shi, Shaofen Xu, Haoyu Cheng, Lihong Wu, Janak L. Pathak, Zhichao Zheng
Summary: This review summarizes the regulatory role of non-coding RNAs in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and discusses their prospects in clinical bone tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Physical
Cheng-Hu Hu, Bing-Dong Sui, Jin Liu, Lei Dang, Ji Chen, Chen-Xi Zheng, Songtao Shi, Na Zhao, Min-Yan Dang, Xiao-Ning He, Li-Qiang Zhang, Ping-Ping Gao, Nan Chen, Hui-Juan Kuang, Kai Chen, Xiao-Lin Xu, Xiao-Rui Yu, Ge Zhang, Yan Jin
Summary: Sympathetic neurostimulation through beta(1/2)-adrenergic receptor signaling triggers the transcription of miR-21 in osteoblasts, which is then transferred to osteoclast progenitors via exosomes to dictate osteoclastogenesis. Pharmacological inhibition of exosome release can suppress this miR-21 transfer and ameliorate bone loss under stress-induced depression conditions.
Review
Pharmacology & Pharmacy
Hewen Jiang, Zongkang Zhang, Yuanyuan Yu, Hang Yin Chu, Sifan Yu, Shanshan Yao, Ge Zhang, Bao-Ting Zhang
Summary: This article reviews the currently available or under study DKK1 inhibitors and discusses future research prospects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Xiao Lin, Fang Xu, Ke-Wen Zhang, Wu-Xia Qiu, Hui Zhang, Qiang Hao, Meng Li, Xiao-Ni Deng, Ye Tian, Zhi-Hao Chen, Ai-Rong Qian
Summary: In this study, the function of acacetin on bone remodeling and H type vessel formation was explored. Acacetin was found to inhibit osteoclast formation and bone resorption, as well as promote angiogenesis and migration of endothelial progenitor cells (EPCs). Furthermore, acacetin was shown to prevent bone loss in mice through inhibition of osteoclast function and promotion of type H vessel formation via Akt/GSK3 beta and NF-kappa B signaling pathway, making it a potential therapeutic agent for the treatment of osteoporosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Bingdong Sui, Jin Liu, Chenxi Zheng, Lei Dang, Ji Chen, Yuan Cao, Kaichao Zhang, Lu Liu, Minyan Dang, Liqiang Zhang, Nan Chen, Tao He, Kun Xuan, Fang Jin, Ge Zhang, Yan Jin, Chenghu Hu
Summary: This study reveals the crucial role of sympathetic cues in regulating bone homeostasis and neurostress-induced bone loss. The research focuses on the impact of sympatho-adrenergic activation on osteoclastogenesis and identifies miR-21 as a key regulator in this process. Targeted inhibition of miR-21 or suppression of osteoclastogenesis using drugs can protect bone against stress-induced osteopenias.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yuanyuan Yu, Luyao Wang, Shuaijian Ni, Dijie Li, Jin Liu, Hang Yin Chu, Ning Zhang, Meiheng Sun, Nanxi Li, Qing Ren, Zhenjian Zhuo, Chuanxin Zhong, Duoli Xie, Yongshu Li, Zong-Kang Zhang, Huarui Zhang, Mei Li, Zhenlin Zhang, Lin Chen, Xiaohua Pan, Weibo Xia, Shu Zhang, Aiping Lu, Bao-Ting Zhang, Ge Zhang
Summary: This study demonstrates that sclerostin has different mechanisms in protecting the cardiovascular system and inhibiting bone formation. By targeting sclerostin loop3, a drug can promote bone formation without affecting the cardiovascular system.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Lihui Chen, Gao Gao, Li Shen, Hua Yue, Ge Zhang, Zhenlin Zhang
Summary: Sclerostin, a secreted inhibitor of Wnt/beta-catenin signaling, is mainly produced by osteocytes and plays an important role in bone remodeling. This study investigated the association between serum sclerostin levels and bone turnover marker in patients with osteogenesis imperfecta (OI), X-linked hypophosphatemia (XLH), and Paget's disease of bone (PDB). The results suggest that sclerostin may serve as a biomarker for OI, XLH, and PDB.
Review
Cell Biology
Zhihao Chen, Ying Huai, Wenjing Mao, Xuehao Wang, Kang Ru, Airong Qian, Hong Yang
Summary: Liquid-liquid phase separation plays a vital role in the formation of membraneless organelles in eukaryotic cells and the precise regulation of cellular functions. It is also associated with the development of metabolic diseases such as type 2 diabetes mellitus, Alzheimer's disease, and metabolic bone diseases.
Article
Biochemistry & Molecular Biology
Jiangfan Han, Ren Na, Ningning Zhao, Xiaofeng Yuan, Linke Fu, Jianmei Jing, Airong Qian, Weiliang Ye
Summary: In this study, dextran sulfate-cisaconitic anhydride-dexamethasone (DXM@DS-cad-DXM) micelles were prepared to selectively target activated macrophages for the treatment of rheumatoid arthritis (RA). DXM@DS-cad-DXM micelles demonstrated potent targeting ability and anti-inflammatory activity, with effective accumulation observed in an adjuvant-induced arthritis (AIA) rat model. Overall, DXM@DS-cad-DXM micelles show promise as a nanomedicine for targeted therapy of RA.
Article
Engineering, Biomedical
Yipu Zhao, Xudong Deng, Shenxing Tan, Jie Zhang, Jiangfan Han, Xue Wang, Jiawei Pei, Hui Li, Xiaoni Deng, Chong Yin, Dachuan Yin, Ye Tian, Airong Qian
Summary: In this study, a cartilage-targeting nanoparticle was designed to deliver therapeutic agents into cartilage for the treatment of osteoarthritis (OA). The results showed that the nanoparticle had significant deposition in cartilage and subchondral bone, reducing cartilage degeneration and inflammation in OA mice, and improving the microarchitecture of subchondral bone.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yipu Zhao, Ye Tian, Weiliang Ye, Xue Wang, Ying Huai, Qian Huang, Xiaohua Chu, Xudong Deng, Airong Qian
Summary: This study presents a novel approach for treating alopecia by using a combination of hyaluronic acid-based dissolving microneedles and lipid polymer hybrid nanoparticles for localized transdermal miRNA delivery, promoting hair regrowth.
BIOMATERIALS SCIENCE
(2022)
Editorial Material
Cell Biology
Ye Tian, Ai-Ming Yu, Chong Yin, Airong Qian
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Zixiang Wu, Lifang Hu, Kang Ru, Wenjuan Zhang, Xia Xu, Shuyu Liu, Hua Liu, Yunxia Jia, Shujing Liang, Zhihao Chen, Airong Qian
Summary: This study investigates the role of cyclin-dependent kinase 12 (CDK12) in osteoporosis induced by simulated microgravity and the therapeutic effect of CDK12-targeted Ellagic Acid (EA) on osteoporosis. The results demonstrate that CDK12-targeted EA efficiently improves osteoporosis by promoting osteoblast differentiation.
Article
Orthopedics
Kewen Zhang, Wuxia Qiu, Hui Li, Jun Li, Pai Wang, Zhihao Chen, Xiao Lin, Airong Qian
Summary: MACF1 plays a positive regulatory role in osteogenesis and bone formation in MSCs through the TCF4/miR-335-5p signaling pathway in SOP, suggesting it as a potential therapeutic target for SOP.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Veterinary Sciences
Teng Tu, Maonan Pang, Dike Jiang, You Zhou, Xulong Wu, Xueping Yao, Yan Luo, Zexiao Yang, Meishen Ren, Aiping Lu, Ge Zhang, Yuanyuan Yu, Yin Wang
Summary: It is important for scientists to be able to communicate their work in a simple and concise manner, especially in an open-access online journal. The simple summary should contain a clear statement of the problem addressed, the aims and objectives, pertinent results, conclusions from the study, and their societal value. It should be written for a general audience, without technical terms or abbreviations. Submissions without a simple summary will be rejected.
VETERINARY SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Anna Hau-Yee Kong, Aston Jiaxi Wu, Olivia Ka-Yi Ho, Maggie Ming-Ki Leung, Alexis Shiying Huang, Yuanyuan Yu, Ge Zhang, Aiping Lyu, Min Li, King-Ho Cheung
Summary: Neuroinflammation is a precursor to neurodegenerative diseases, and targeting it with aptamers offers potential diagnostic and therapeutic applications. This review summarizes the use of aptamers for diagnosing and treating inflammatory biomolecules and cells associated with neurodegenerative diseases. It also discusses the potential of short nucleotides for targeted brain delivery and the challenges of using aptamers as therapeutic agents. Overall, this review assesses the potential of aptamers as a pioneering approach for CNS target delivery and the treatment of neuroinflammation and neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
