4.7 Article

Comparison of liquid hot water (LHW) and high boiling alcohol/water (HBAW) pretreatments for improving enzymatic saccharification of cellulose in bamboo

期刊

INDUSTRIAL CROPS AND PRODUCTS
卷 107, 期 -, 页码 139-148

出版社

ELSEVIER
DOI: 10.1016/j.indcrop.2017.05.035

关键词

High-boiling point alcohol/water (HBAW) pretreatment; Liquid hot water (LHW); Substrate enzymatic digestibility; Non-effective adsorption; Glucose yield

资金

  1. National Natural Science Foundation of China [31300495]
  2. Fujian Provincial Department of Education [JB13033, JA15181, JA14098]
  3. New Century Excellent Talents Supporting Plan [MM [2015]54]
  4. Department of Forestry
  5. Department of Science and Technology [2014J05028]
  6. Postdoctoral Science Foundation of China [2015M571955]
  7. National College Students Innovation and Entrepreneurship Training Program [201610389020]

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Liquid hot water (LHW) and high-boiling point alcohol/water (HBAW) pretreatments were compared to improve enzymatic saccharification of cellulose in moso bamboo. When pretreatments were conducted at 200 degrees C for 40 min, HBAW (20% (v/v) 1,4- butanediol (BDO)) induced substrate enzymatic digestibilities (SEDs) at low (5 FPU g(-1) glucan) and high (20 FPU g(-1) glucan) cellulase dosages were 47.1% and 82.3%, which were higher than that of LHW by about 46% and 14%, respectively. The analyses of solid substrates and hydrolysates demonstrated that deposited lignin played a dominating role over other factors including total pore volume, cellulose crystallinity and molecular weight in affecting SED during LHW pretreatment. After optimizing the ratio of BDO to water to be 10% (v/v), a SED and glucose yield of 91.6% and 81.5%, respectively, were obtained by HBAW pretreatment conducted at 200 degrees C for 40 min while cellulase dosage was charged at 20 FPU g(-1) glucan. Under the same conditions but without BDO addition, LHW only resulted in a SED and glucose yield of 72.2% and 62.3%, respectively. Due to no mineral acid addition and low loadings of BDO, HBAW pretreatment has the potential to overcome the drawbacks of LHW pretreatment and become a green, non-expensive and high efficient pretreatment.

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